Several factors influence women's perception of their genitalia. Understanding the elements associated with genital self‑image (GSI) can significantly contribute to enhancing women's sexual experiences. Therefore, the present study aimed to identify the predictors of GSI in Iranian women. This online cross-sectional study was conducted on 722 healthy married reproductive-aged women with records in Amol-Iran health centers during 2022. Participants were selected using a random sampling method from all the healthcare centers. Data were collected through a checklist of socio-demographic and obstetrics information, the Female Genital Self-Image Scale (FGSI), the 6-item Female Sexual Function Index (FSFI-6), the Female Sexual Distress Scale - revised (FSDS-R), and the Multidimensional Sexual Self-Concept Questionnaire (MSSCQ) for assessing sexual esteem and sexual satisfaction. The general linear model was employed to assess the impact of each independent variable (socio-demographic and obstetrics characteristics, sexual function, sexual distress, sexual esteem, and sexual satisfaction) on the dependent variable (GSI). The mean score of GSI was 21.70 ± 3.82. Based on the Pearson correlation test, there were significant correlations between GSI and sexual function, sexual esteem, sexual satisfaction, and sexual distress (r = 0.20, r = 0.43, r = 0.42, r = -0.28 respectively; p < 0.001). Using the adjusted general linear model, with the increasing scores of sexual esteem and sexual satisfaction, the GSI score also increased (β = 0.19, 95% CI: 0.10 to 0.27, β = 0.14, 95% CI: 0.06 to 0.22, respectively; p < 0.001). Additionally, age, abnormal vaginal discharge, genital feedback from partners, and satisfaction with body image were identified as other predictors of GSI. Based on the study results, it was observed that sexual esteem, sexual satisfaction, and certain socio-demographic variables served as predictors of GSI. Consequently, considering the potential impact of GSI on various aspects of women's lives, healthcare providers can enhance their counseling techniques to improve GSI by gaining an appropriate understanding of the factors that influence it.