Within-subject Comparisons Research Articles

No difference between slow oscillation up- and down-state cueing for memory consolidation during sleep.

The beneficial effects of sleep for memory consolidation are assumed to rely on the reactivation of memories in conjunction with the coordinated interplay of sleep rhythms like slow oscillations and spindles. Specifically, slow oscillations are assumed to provide the temporal frame for spindles to occur in the slow oscillations up-states, enabling a redistribution of reactivated information within hippocampal-neocortical networks for long-term storage. Memory reactivation can also be triggered externally by presenting learning-associated cues (like odours or sounds) during sleep, but it is presently unclear whether there is an optimal time-window for the presentation of such cues in relation to the phase of the slow oscillations. In the present within-subject comparison, participants (n=16) learnt word-pairs visually presented with auditory cues of the first syllable. These syllables were subsequently used for real-time cueing either in the up- or down-state of endogenous slow oscillations. Contrary to our hypothesis, we found differences in memory performance neither between up- and down-state cueing, nor between word-pairs that were cued versus uncued. In the up-state cueing condition, higher amounts of rapid eye movement sleep were associated with better memory for cued contents, whereas higher amounts of slow-wave sleep were associated with better memory for uncued contents. Evoked response analyses revealed signs of cue processing in both conditions. Interestingly, both up- and down-state cueing evoked a similar spindle response with the induced slow oscillations up-state at ~1000ms post-cue. We speculate that our cueing procedure triggered generalised reactivation processes that facilitated the consolidation of both cued and uncued memories irrespective of the slow oscillation phase.

Prisoner knowledge about head injury is Improvedby brief psychoeducation

ABSTRACT Introduction The high prevalence of head injury (HI) in prisoners and its association with offending indicates a need for interventions. However, there is little evidence and none for the effectiveness of psychoeducation in improving prisoner knowledge about HI and its effects. Methods Small groups of males in two Scottish prisons underwent a 1 hour psychoeducation session delivered by PowerPoint and combined with question and answer, video clips and a booklet about HI. A pre-post intervention design was used to assess knowledge about HI from vignettes. Participants indicated effects of HI using unprompted free recall and then with a questionnaire (the Symptom Checklist; SCL), pre-education (n = 34), post-education (n = 19) and at 4-week follow-up (n = 11). Free recall was scored using symptom lists from national guidelines (FR-SIGN) or the SCL (FR-SCL). Within-subject comparisons were made between pre-intervention, post-intervention and follow-up scores. Results Knowledge about HI significantly increased pre- to post-education for FR-SIGN (d = 0.91; 95% CI 0.62, 2.53) and FR-SCL (d = 0.99; 95% CI 0.95, 4.00) without decrement at follow-up (FR-SIGN d = 1.27; 95% CI 0.53, 2.56; FR-SCL r = 0.60). Scores on the SCL did not change over time (p > .05). Conclusion Prisoner knowledge about HI was improved by brief psychoeducation suitable for delivery in prisons.

Emergency department consultations for respiratory symptoms revisited: exploratory investigation of longitudinal trends in patients\u2019 perspective on care, health care utilization, and general and mental health, from a multicenter study in Berlin, Germany

BackgroundOnly few studies of emergency department (ED) consulters include a longitudinal investigation. The EMACROSS study had surveyed 472 respiratory patients in eight inner-city EDs in Berlin in 2017/2018 for demographic, medical and consultation-related characteristics. This paper presents the results of a follow-up survey at a median of 95 days post-discharge. We aimed to explore the post hoc assessment of ED care and identify potential longitudinal trends.MethodsThe follow-up survey included items on satisfaction with care received, benefit from the ED visit, potential alternative care, health care utilization, mental and general health, and general life satisfaction. Univariable between-subject and within-subject statistical comparisons were conducted. Logistic regression was performed for multivariable investigations of determinants of dropout and of retrospectively rating the ED visit as beneficial.ResultsFollow-up data was available for 329 patients. Participants of lower education status, migrants, and tourists were more likely to drop out. Having a general practitioner (GP), multimorbidity, and higher general life satisfaction were determinants of response. Retrospective satisfaction ratings were high with no marked longitudinal changes and waiting times as the most frequent reason for dissatisfaction. Retrospective assessment of the visit as beneficial was positively associated with male sex, diagnoses of pneumonia and respiratory failure, and self-referral. Concerning primary care as a viable alternative, judgment at the time of the ED visit and at follow-up did not differ significantly. Health care utilization post-discharge increased for GPs and pulmonologists. Self-reported general health and PHQ-4 anxiety scores were significantly improved at follow-up, while general life satisfaction for the overall sample was unchanged.ConclusionsMost patients retrospectively assess the ED visit as satisfactory and beneficial. Possible sex differences in perception of care and its outcomes should be further investigated. Conceivable efforts at diversion of ED utilizers to primary care should consider patients’ views regarding acceptable alternatives, which appear relatively independent of situational factors. Representativeness of results is restricted by the study focus on respiratory symptoms, the limited sample size, and the attrition rate.Trial registrationGerman Clinical Trials Register (DRKS00011930); date: 2017/04/25.

Auditory brainstem response in unilateral tinnitus patients: does symmetrical hearing thresholds and within-subject comparison affect responses?

In recent studies, cochlear synaptopathy has been suggested as a potential pathophysiology mechanism for tinnitus, which occurs in individuals with normal hearing thresholds. Auditory Brainstem Response (ABR) is a noninvasive method frequently used in the literature to evaluate cochlear synaptopathy in tinnitus patients. However, possible factors such as high-frequency pure-tone hearing thresholds, age, gender, and head characteristics that may affect ABR were not considered sufficiently in previous studies. Therefore, the present study aims to evaluate tinnitus ears and non-tinnitus ears with ABR in unilateral chronic tinnitus patients with symmetrical hearing. Twenty unilateral chronic tinnitus patients having normal pure-tone average with symmetrical hearing thresholds was included in the study. Subjects were evaluated with 0.25-16kHz pure-tone audiometry, Tinnitus Handicap Inventory (THI) and ABR were administered. All ears were evaluated monaurally using click stimuli at 80dB nHL, alternating polarity (21.1 rate/s, 2000 sweeps). Wave I amplitude of the ABR and the ratio of III/I, V/I, and V/III wave amplitudes from tinnitus ears was higher than non-tinnitus ears. At the same time, there was a positive correlation between THI and V-I and V-III interpeak latency range, and a negative correlation between V/III wave amplitude ratio. ABR can be used as an evaluation method to provide evidence that the neural organizations of individuals with chronic tinnitus differ in certain regions in their auditory pathways. The correlation between THI and ABR findings suggests that there may be a connection between tinnitus distress and the neural organization of the auditory system.

Detecting deception using comparable truth baselines

ABSTRACT Baselining – comparing the statements of interest to a known truthful statement by the same individual – has been suggested to improve lie detection accuracy. A potential downside of baselining is that it might influence the characteristics of a subsequent statement, as was shown in previous studies. In our first experiment we examined this claim but found no evidence that a truthful baseline influenced the characteristics of a subsequent statement. Next, we investigated whether using a truthful baseline statement as a within-subject comparison would improve lie detection performance by investigating verbal cues (Experiment 1) and intuitive judgements of human judges (Experiment 2). Our exploratory analyses showed that truth tellers included more auditory and temporal details in their target statement than in their baseline than liars. Observers did not identify this verbal pattern. Exposure to a truthful baseline statement resulted in a lower truth accuracy but no difference in lie accuracy.

Convergent Coding of Recent and Remote Fear Memory in the Basolateral Amygdala

BackgroundIn both rodents and humans, the basolateral amygdala (BLA) is essential for encoding and retrieving conditioned fear memories. Although the BLA is a putative storage site for these memories, recent evidence suggests that they become independent of the BLA with the passage of time. MethodsWe systematically examined the role for the BLA in the retrieval of recent (1 day) and remote (2 weeks) fear memory using optogenetic, electrophysiological, and calcium imaging methods in male and female Long-Evans rats. Critically, we used a behavioral design that permits within-subjects comparison of recent and remote memory at the same time point; freezing behavior served as the index of learned fear. ResultsWe found that BLA c-Fos expression was similar after the retrieval of recent or remote fear memories. Extracellular single-unit recordings in awake, behaving animals revealed that single BLA neurons exhibit robust increases in spike firing to both recent and remote conditioned stimuli. Fiber photometry recordings revealed that these patterns of activity emerge from principal neurons. Consistent with these results, optogenetic inhibition of BLA principal neurons impaired conditioned freezing to both recent and remote conditioned stimuli. There were no sex differences in any of the measures or manipulations. ConclusionsThese data reveal that BLA neurons encode both recent and remote fear memories, suggesting substantial overlap in the allocation of temporally distinct events. This may underlie the broad generalization of fear memories across both space and time. Ultimately, these results provide evidence that the BLA is a long-term storage site for emotional memories.

Nightmares in People with COVID-19: Did Coronavirus Infect Our Dreams?

IntroductionA growing number of studies have demonstrated that the coronavirus disease-19 (COVID-19) pandemic has severely affected sleep and dream activity in healthy people. To date, no investigation has examined dream activity specifically in COVID-19 patients.MethodsAs part of the International COVID-19 Sleep Study (ICOSS), we compared 544 COVID-19 participants with 544 matched-controls. A within-subjects comparison between pre-pandemic and pandemic periods computed separately for controls and COVID-19 participants were performed on dream recall and nightmare frequency (DRF; NF). Also, non-parametric comparisons between controls and COVID-19 participants were carried out. Further, we compared psychological measures between the groups collected during pandemic. Ordinal logistic regression to detect the best predictors of NF was performed.ResultsWe found that people reported greater dream activity during the pandemic. Comparisons between controls and COVID-19 participants revealed a) no difference between groups concerning DRF in the pre-pandemic period and during the pandemic; b) no difference between groups concerning nightmare frequency in the pre-pandemic period; and c) COVID-19 participants reported significantly higher NF than controls during pandemic (p = 0.003). Additionally, we showed that a) anxiety, depression, post-traumatic stress-disorder (PTSD) symptom scores were higher in COVID-19 participants than controls; and b) quality of life and health as well as wellbeing (WHO-5) scores were significantly higher in controls than COVID-19 participants. Finally, ordinal logistic regression indicates that DRF (p < 0.001), PTSD (p < 0.001), anxiety (p = 0.018), insomnia (p = 0.039), COVID-19 severity (p = 0.014), sleep duration (p = 0.003) and age (p = 0.001) predicted NF.DiscussionOur work shows strong associations between increased nightmares in those reporting having had COVID-19. This suggests that the more that people were affected by COVID-19, the greater the impact upon dream activity and quality of life.

The Fingerprint-Like Pattern of Nocturnal Brain Activity Demonstrated in Young Individuals is Also Present in Senior Adulthood.

PurposeThe quantitative sleep EEG has been considered as electroencephalographic “fingerprint”, ie, it is stable within but differs between individuals. So far, however, almost all studies addressing this aspect have been conducted in young men. It was therefore of interest to know whether the sleep EEG fingerprint concept holds true in older samples of both sexes.Patients and MethodsData from three different subsamples of 30 healthy individuals each were reused for the present secondary analysis (young men (YM) = 25.6 ± 2.4 years, elderly men (EM) = 69.1 ± 5.5 years, elderly women (EW) = 67.8 ± 5.7 years). Individuals slept ten times in the sleep laboratory, resulting in a total of 900 study nights. However, to avoid misinterpretation due to intervention-related changes in sleep EEG power spectra, only the 3 sham nights without any intervention were included, reducing the datasets to 270. To determine stability of NREM sleep EEG power spectra between sham night pairs, within- and between-subject Manhattan distance measures were computed separately by sample.ResultsRegardless of subsample and sham night pair, lowest distance measures, ie, largest similarity, were observed for within-subject power spectra comparisons (range of mean distance measures for EW from 3.82 to 4.06, for EM from 3.55 to 3.63, and for YM from 3.04 to 3.62). Moreover, intraindividual similarity did not differ substantially between samples. Between-subject power spectra distance measures were considerably larger (range of mean distance measures for EW from 12.95 to 13.15, for EM from 12.21 to 12.57, and for YM from 10.33 to 10.78) and varied significantly between young and elderly individuals.ConclusionThe present results support the view that the sleep EEG power spectrum is an individual trait-like characteristic that remains unique up until old age. This finding may help to increase the sensitivity in measuring intervention effects.

Verification of a Comfort-Enhanced Liquid Cooling Vest

Firefighter uniforms provide thermal protection and block radiant as well as high-temperature heat. However, they limit cooling and the dissipation of internal heat from the body. This study attempted to improve firefighter uniforms by developing and evaluating a comfortable and thermally balanced design. This study recruited six male college students for a within-subject comparison of vests with and without active liquid cooling. The participants used a questionnaire to report their comfort level in each body part while performing various motions. A biomechanical analysis was performed to objectively evaluate the comfort level of the cooling vest before and after the test. Subsequently, the participants’ blood pressure and ear as well as skin temperature were measured as they ran on a treadmill. The participants also responded to a questionnaire regarding their thermal perception. The results revealed that the cooling vest was comfortable and flexible. The data from the psychological questionnaire indicated that the participants were satisfied with the warmth, coolness, and other various aspects of the cooling vest. Moreover, the cooling vest positively affected the wearer’s microclimate and provided a comfortable thermal balance. The current findings demonstrate the feasibility of using human-factors-engineering-based objective verification methods for designing products.

Extended-release buprenorphine outcomes among treatment resistant veterans

ABSTRACT Background: Buprenorphine Extended-Release (BUP-XR) is an FDA approved, monthly subcutaneous injection for opioid use disorder. This formulation provides an alternative for patients who have difficulty adhering to daily sublingual buprenorphine; however, its cost may be prohibitive compared to other medication alternatives. Objectives: The objective of this project was to evaluate the effectiveness of BUP-XR and provide a rationale for its utilization in health care facilitates. Methods: Across two VA outpatient clinics, twenty-six (22 male, 4 female) clinically complex patients received at least one BUP-XR injection between December 1, 2018 and April 1, 2020. The sample was high risk for hospital admission and mortality. Data was collected retrospectively from the medical records. Outcomes examined included: medication adherence, urine drug and alcohol screenings, emergency department visits, hospital admissions, and housing status. Within-subject comparisons were made between the six month period prior to and following the first injection. Results: Treatment retention was robust, as 81% of the sample received six or more monthly BUP-XR injections. Most patients (77%) maintained 300 mg dosage and a majority (70%) missed or were late for at least one injection. BUP-XR was associated with reduction in: emergency department visits, days of hospitalization, non-prescribed opioid use, and homelessness. Mortality rate was 23%. Conclusions: BUP-XR with a flexible dosing schedule and a nonstandard default dose of 300 mgs resulted in robust retention, provided effective treatment of OUD, and reduced health care utilization for these complex patients with high mortality risk. However, due to the lack of statistical significance generalization of these findings is limited.

Do local craft beverages taste better? An investigation into the halo effect

ABSTRACT In the United States, many consumers seek out local consumables, a phenomenon known as neolocalism. This behavior is exemplified by interest in local craft beer, which often uses differentiated local ingredients. Although popular, it is not clear if they taste better, compared to mass-produced craft beers or if a halo effect exists. Thus, this study measures customers’ expectations, perceptions, and satisfaction with the taste of a local craft beer, compared to a mass-produced craft beer using an experimental within-subject comparison. Results indicate that expectations were higher for the local craft beer, but satisfaction levels were higher for the non-local craft beer. Overall, for researchers, this study demonstrates the presence of a halo effect in some local craft beer contexts. For practitioners, findings allow them to gain greater understanding of customers’ expectations, perceptions, and satisfaction levels with relation to the taste of local craft and craft beer.

Exercising With a Six Pack in Virtual Reality: Examining the Proteus Effect of Avatar Body Shape and Sex on Self-Efficacy for Core-Muscle Exercise, Self-Concept of Body Shape, and Actual Physical Activity.

This study investigates the Proteus effect from the first-person perspective and during avatar embodiment in actual exercise. In addition to the immediate measurements of the Proteus effect, prolonged effects such as next-day perception and exercise-related outcomes are also explored. We theorized the Proteus effect as altered perceived self-concept and explored the association between virtual reality (VR) avatar manipulation and self-concept in the exercise context. While existing studies have mainly investigated the Proteus effect in a non-VR environment or after VR embodiment, we aim to contribute to the literature by addressing this concern to explore how the Proteus effect works in actual VR exercise. Through a 2 (avatar body shape: with a six pack vs. normal) × 2 (sex: male vs. female) between-subject experiment, the results partially support the Proteus effect. Regarding actual physical activity, embodying an avatar with a six pack during exercise creates fewer body movements. No significant effect was found for perceived exertion. We also explored the role of sex as a potential moderator in the association of the Proteus effect on exercise outcomes. The Proteus effect was supported by immediate and next-day self-efficacy for core-muscle exercise only among female participants. The between-subject design allowed us to probe how avatar manipulation of muscular body shape with a six pack as opposed to normal body shape influences participants’ self-concept and exercise outcomes, as limited VR studies have employed within-subject comparisons. This also contributes to the literature by providing an upward comparison (e.g., muscular with a six pack vs. normal) as opposed to the previous downward comparison regarding body fitness (e.g., normal vs. obese). The overall results supported the Proteus effect in the context of core-muscle exercise when comparing normal and ideal body shape avatars. However, the Proteus effect as an altered self-concept and its effects on self-efficacy for exercise were supported among females but not males. Whereas the female participants who embodied avatars with a six pack associated themselves more with the muscular concept than other people, the male participants who embodied avatars with a six pack perceived themselves as more normal than others. Theoretical and practical implications are discussed.

A within-subject comparison of remote and in-laboratory methods for auditory detection studies

While the gold standard of psychoacoustic research involves tightly controlled, laboratory-based inquiry, it has been necessary to develop protocols to conduct experiments on auditory perception outside of the lab without sacrificing experimental rigor and data quality. Outside of the lab, there is the potential for differences in acoustic environment across subjects, as well as increased auditory and visual distractions. The present study compares the difference in performance on a masked auditory detection experiment within the same cohort of subjects across two platforms of varying experimental control: (1) an in-lab, computer-based experiment conducted within a sound booth; and (2) a remote, tablet-based experiment conducted with provided noise-isolating headphones in an uncontrolled—but measured—acoustic environment. Comparisons in performance across experimental platforms and settings will be discussed, including the influence of a concurrent visual task to encourage task focus, and the effect of background noise levels on performance in the remote setting. Results suggest that there was little influence of the setting and equipment on subjects’ performance in this masked auditory detection study, as similar results were obtained in both settings. This work aims to provide guidance on best-practices for conducting remote acoustic studies without a detriment to data quality.

Influence of implant location on the prevalence of peri-implantitis in patients with periimplantitis: a within-subjects comparison of healthy and peri-implantitis affected implants

The aim of this study was to analyze the influence of implant location on the prevalence of peri-implantitis by within-subjects comparison of healthy and peri-implantitis affected implants in patients with peri-implantitis.To be included for this study, the patient should have at least one peri-implantitis affected implant and one healthy implant. Following clinical and radiographic assessments for peri-implantitis, 56 patients with 89 peri-implantitis affected implants and 249 healthy implants were included in this study. Influences of implant location variables including jaw position, tooth type, tooth position, and tooth adjacent to the implant on the prevalence of peri-implantitis were assessed using a multilevel analysis. Implants placed in the maxillary jaw showed 2 times higher frequency of peri-implantitis than those placed in the mandibular jaw. Implants with absence of adjacent tooth were twice more likely to be affected by peri-implantitis than those with presence of an adjacent tooth. Sub-group analysis for the combination of jaw and position variables revealed that implants in the maxillary anterior position showed significantly higher prevalence rate of peri-implantitis than those in the mandibular anterior position. Within limitations of the present study, patients showed significant increase in the prevalence rate of peri-implantitis when the implant was placed in the maxillary jaw or when tooth adjacent to the implant was absent. In addition, the presence of tooth seemed to provide positive effect on the occurrence of peri-implantitis at the adjacent implant.

Multi‐band FMRI compromises detection of mesolimbic reward responses

Recent innovations in Functional Magnetic Resonance Imaging (FMRI) have sped data collection by enabling simultaneous scans of neural activity in multiple brain locations, but have these innovations come at a cost? In a meta-analysis and preregistered direct comparison of original data, we examined whether acquiring FMRI data with multi-band versus single-band scanning protocols might compromise detection of mesolimbic activity during reward processing. Meta-analytic results (n = 44 studies; cumulative n = 5005 subjects) indicated that relative to single-band scans, multi-band scans showed significantly decreased effect sizes for reward anticipation in the Nucleus Accumbens (NAcc) by more than half. Direct within-subject comparison of single-band versus multi-band scanning data (multi-band factors = 4 and 8; n = 12 subjects) acquired during repeated administration of the Monetary Incentive Delay task indicated that reductions in temporal signal-to-noise ratio could account for compromised detection of task-related responses in mesolimbic regions (i.e., the NAcc). Together, these findings imply that researchers should opt for single-band over multi-band scanning protocols when probing mesolimbic responses with FMRI. The findings also have implications for inferring mesolimbic activity during related tasks and rest, for summarizing historical results, and for using neuroimaging data to track individual differences in reward-related brain activity.

Abstract P237: Alterations In Renal Sodium Handling Proteins Determined By Urinary Extracellular Vesicle Analysis During Dietary Sodium Modulation And In Primary Aldosteronism

Sodium handling is altered during extremes in dietary sodium intake or in pathologic conditions such as primary aldosteronism. Urinary extracellular vesicles (uEVs) are excreted by all cell types along the nephron, are enriched for membrane proteins, and could provide insight into renal physiology in humans. We assessed uEV protein excretion of renal epithelial proteins relevant to sodium handling from 54 volunteers with pre-hypertension on high (HS, 200 mmol/d) and low (LS, 10 mmol/d) sodium diet for 5-7 days. To compare pathologic versus physiologic responses to aldosterone excess, samples were collected from 9 patients with primary aldosteronism (PA). uEVs were isolated by ultracentrifugation and trypsin digested proteins were quantified using multiple reaction monitoring mass spectrometry using isotopically labeled standards, normalized to the uEV-specific marker CD9. Wilcoxon signed rank and rank sum tests were used for within-subject and between-subject comparisons, respectively. LS diet altered uEV excretion of pendrin (4.0, expressed as fold-change vs HS), aquaporin-2 (-2.03), TRPV5 (-1.91), NCC (1.51), OSR1 (1.42; P &lt;0.01 for all), and SGLT2 (1.20, P =0.042). In primary aldosteronism, expression of ENaC-α, ENaC-β, and ENaC-γ, NCC, SGLT2, and OSR-1 were increased to a greater extent compared to during LS diet ( P &lt;0.05 for each), whereas AQP2 and TRPV5 were similar to HS diet ( Figure ). Expression of uEV proteins relevant to renal tubular sodium handling are dynamically altered by dietary sodium restriction and aldosterone excess. Further studies could identify patterns specific to pathologic versus physiologic aldosterone responses.

P–143 Improved embryonic development and utilization rates with EmbryoScope: A within-subject comparison versus a benchtop incubator

Abstract Study question In consecutive intracytoplasmic sperm injection (ICSI) cycles, is embryonic development in EmbryoScope better than the previous one obtained in a benchtop (G–185) incubator? Summary answer Embryonic development, and oocyte and embryo utilization rates (OUR and EUR) are significantly improved in the EmbryoScope, as compared to G–185. What is known already The time-lapse imaging (TLI) system, which allows a non-invasive continuous assessment of embryo morphokinetics parameters in a closed culture system has been developed, promising improved embryo development by reducing oscillations in pH, humidity and temperature. To investigate this hypothesis, one study has already compared embryonic development in a TLI versus a benchtop incubator. However, It has never been investigated whether embryonic development can be improved within-subject, by changing from benchtop incubator in the first intracytoplasmic sperm injection (ICSI) cycle to the EmbryoScope, a TLI incubator, in the following ICSI cycle, and that was the objective of the present study. Study design, size, duration This study had a retrospective within-subject design, in which each cycle served as its own control. Data were obtained via chart review of patients undergoing ICSI in a private university–affiliated IVF center that fulfilled the following criteria: second ICSI attempt in which embryos had been cultured in a TLI incubator system (TLI group, n = 71), preceded by a first ICSI attempt in which embryos had been cultured in a conventional incubator (Control group, n = 71). Participants/materials, setting, methods Embryonic development up to the fifth day of development, OUR (transferred embryos plus frozen embryos per retrieved oocytes) and EUR (transferred embryos plus frozen embryos per fertilized oocytes) were compared between the groups using generalized linear models followed by Bonferroni post hoc. The post hoc achieved power was 82.6%, considering the sample size, the effect size obtained for blastocyst development rate and 5% significance level. Main results and the role of chance There were significant differences in fertilization rate (76.0% ± 1.3 vs. 80.0% ± 1.4, p = 0.044, OR: 1.051, CI: 1.001 – 1.103), non-fertilization rate (14.8% ± 0.6 vs. 6.3% ± 0.4, p &amp;lt; 0.001, OR: 0.424, CI: 0.370 – 0.486), day–2 non-cleavage rate (3.8% ± 0.2 vs. 1.1% ± 0.1, p &amp;lt; 0.001, OR: 0.285, CI: 0.234 – 0.347), blastocyst development rate (40.9% ± 1.1 vs. 55.6% ± 1.3, p &amp;lt; 0.001, OR: 1.358, CI: 1.267 – 1.456), frozen blastocyst rate (31.8% ± 0.8 vs. 37.0% ± 0.9, p &amp;lt; 0.001, OR: 1.163, CI: 1. 085 – 1.248), OUR (40.7% ± 1.0 vs. 50.2% ± 1.1, p &amp;lt; 0.001, OR: 1.232, CI: 1.155 – 1.314), and EUR (52.4% ± 1.1 vs. 66.6% ± 1.2, p &amp;lt; 0.001, OR: 1.269, CI: 1.202 - 1.341), all in favor of TLI group. Pregnancy rate (30.2% vs. 30.8%, p = 0.940), implantation rate (24.6% ± 40.0 vs. 26.1% ± 41.6, p = 0.830), and miscarriage rate (21.1% vs. 15.0%, p = 0.622) were similar between Control and TLI groups, respectively. Limitations, reasons for caution (i) Different culture dishes were used in each system; (ii) it is not possible to confirm how much of the embryonic improvement was due to the culture conditions; (iii) the study design is not ideal for the comparison of clinical outcomes and, also, underpowered to do so. Wider implications of the findings: Even though the clinical outcomes were similar between the groups, the results may also lead to higher cumulative pregnancy outcomes following embryo thawing and transfer. Trial registration number Not applicable

Frontal midline theta differentiates separate cognitive control strategies while still generalizing the need for cognitive control

Cognitive control processes encompass many distinct components, including response inhibition (stopping a prepotent response), proactive control (using prior information to enact control), reactive control (last-minute changing of a prepotent response), and conflict monitoring (choosing between two competing responses). While frontal midline theta activity is theorized to be a general marker of the need for cognitive control, a stringent test of this hypothesis would require a quantitative, within-subject comparison of the neural activation patterns indexing many different cognitive control strategies, an experiment lacking in the current literature. We recorded EEG from 176 participants as they performed tasks that tested inhibitory control (Go/Nogo Task), proactive and reactive control (AX-Continuous Performance Task), and resolving response conflict (Global/Local Task-modified Flanker Task). As activity in the theta (4–8 Hz) frequency band is thought to be a common signature of cognitive control, we assessed frontal midline theta activation underlying each cognitive control strategy. In all strategies, we found higher frontal midline theta power for trials that required more cognitive control (target conditions) versus control conditions. Additionally, reactive control and inhibitory control had higher theta power than proactive control and response conflict, and proactive control had higher theta power than response conflict. Using decoding analyses, we were able to successfully decode control from target trials using classifiers trained exclusively on each of the other strategies, thus firmly demonstrating that theta representations of cognitive control generalize across multiple cognitive control strategies. Our results confirm that frontal midline theta-band activity is a common mechanism for initiating and executing cognitive control, but theta power also differentiates between cognitive control mechanisms. As theta activation reliably differs depending on the cognitive control strategy employed, future work will need to focus on the differential role of theta in differing cognitive control strategies.

A Cross-Cultural Comparison of the Effects of Alcohol Mixed with Energy Drink (AMED) Consumption on Overall Alcohol Consumption and Related Consequences

There is a growing body of scientific literature examining the effects of alcohol mixed with energy drink (AMED) on alcohol consumption and related negative consequences, such as risky behavior or negative health effects. It is unknown whether differences in cultural context may influence these AMED effects. The current cross-cultural study compared the data of N = 6881 students from The Netherlands (N = 4424), UK (N = 1594), and Australia (N = 863). Demographics, alcohol consumption, its consequences, and motives for AMED consumption were assessed. Analyses included (a) between-groups comparison of AMED and alcohol only (AO) consumers, (b) within-subjects comparison of AMED and AO occasions among AMED consumers only, and (c) comparisons between the three countries. The between-groups analysis revealed that AMED consumers drink more alcohol than AO consumers (p < 0.001). AMED consumers differed from AO consumers in many other aspects. For example, AMED consumers were significantly more often a smoker and had higher risk-taking scores. Within subject analysis among AMED consumers showed that significantly less alcohol was consumed on AMED, compared to AO occasions (p < 0.001). These findings were observed for both typical drinking occasions and the past month’s heaviest drinking occasion, and were consistent across the three countries. Comparisons between countries revealed that on both AMED and AO occasions, the UK sample consumed significantly more alcohol than the Australian and Dutch samples. Across countries, neutral motives such as ‘I like the taste’ and ‘I wanted to drink something else’ were the most frequently reported motives for consuming AMED. The most notable difference between the countries was the finding that consuming AMED ‘To get drunk’ was endorsed significantly more often among the UK sample (45.6%) than the Australian (31.2%) and Dutch (8.0%) samples. Negative alcohol-related consequences were significantly less frequently reported for AMED occasions compared to AO occasions. Some country-specific consequences of AMED consumption were observed, but these were more likely related to characteristics of the country and their drinking culture (e.g., the Australian sample reported more often driving a car after AMED consumption compared to the other countries, and this pattern of results was also found for AO occasions). In conclusion, there were limited differences between countries with regard to demographics of consumers and motives for AMED consumption, but the UK sample consumed significantly more alcohol and reported the highest frequency of negative alcohol related consequences. Consistent across countries was the observation that AMED consumers consume significantly less alcohol on their past month heaviest AMED drinking occasion, compared to their past month heaviest AO drinking occasion.

A Yoga Intervention for Young Children: Self-Regulation and Emotion Regulation.

Yoga-based interventions have been implemented in schools and demonstrated promising results on students’ self-regulation outcomes. Nevertheless, there is limited literature on the effects that yoga may have for children in the early primary grades, despite the evidence demonstrating that this is an opportune period in development for early self-regulation. Few studies have focused on young children living in the context of economic difficulty, which can hinder children’s development of self-regulatory skills and educational trajectories. The effects of an eight-week yoga intervention on economically disadvantaged pre-kindergarten and kindergarten children’s self-regulation and emotion regulation were examined via a paired within-subjects comparison study. Nine classrooms were assigned to the yoga intervention (Treatment First, TxFirst; n = 90) or a wait-list control group (Treatment Second, TxSecond; n = 64). All children were assessed at pre-intervention (Time 1), post-intervention assessment for TxFirst (Time 2), and post-intervention assessment for TxSecond (Time 3). Children demonstrated significant predicted gains on a behavioral task of self-regulation and declines in teacher-rated submissive venting and total behavior problems. Implications for future research are discussed, with a focus on including follow-up assessments and multiple dimensions of fidelity of implementation.