Withania Somnifera Root Extract Research Articles

Complementary and Alternative Medicine: Impact of Biofield Energy Treatment on the Physicochemical and Thermal Properties of Withania somnifera Root Extract

Withania somnifera (WS) is a popular health supplement, which is used in many neutraceutical and pharmaceutical formulations for the overall good health, improves the quality of life, along with the prevention and treatment of numerous diseases. In this study, the impact of the Consciousness Energy Healing Treatment (the Trivedi Effect®) on the physicochemical and thermal properties of WS root extract was evaluated using modern analytical techniques. The test sample WS root extract was divided and termed as control and Biofield Energy Treated sample. The control sample did not receive the Biofield Energy Treatment; whereas, the treated WS root extract received the Consciousness Energy Healing Treatment by a well-known Biofield Energy Healer, Dahryn Trivedi. The particle size values of the treated WS root extract powder sample were altered by -7.92% (d10), -5.32% (d50), and -0.36% (d90) 1.02% {D(4,3)}; thus, the specific surface area was significantly increased by 5.31% compared with the control sample. The evaporation temperature and latent heat of evaporation of the treated WS root extract were significantly increased by 11.24% and 21.34%, respectively compared with the control sample. The total weight loss in the treated WS root extract was decreased by 4.3%; however, the residue amount was significantly increased by 25.02% compared with the control sample. The experimental results concluded that the Trivedi Effect®-Consciousness Energy Healing Treatment might have altered particle size, surface area, and thermal properties of WS root extract which would show better solubility, dissolution, absorption, bioavailability, and thermal stability compared to the control sample. This new form of WS root extract would be helpful to design more efficacious neutraceutical/pharmaceutical formulations for rejuvenate the body, restricting the aging process, immune busting, neuro-protection, antioxidant, inflammation, memory loss, insomnia, hepatoprotection, hypoglycemia, gastrointestinal issues, constipation, arthritis, cancer, skin conditions, Alzheimer's, Huntington's, and Parkinson's disorders, etc.

Amelioration of Titanium Dioxide nanoparticles induced injury on the cerebellum of the adult mice by a hydromethanolic root extract of Withania Somnifera

Introduction: Titanium Dioxide (TiO2) nanoparticles have potential risks to human health. They were able to enter brain to be detected in the cerebral cortex, cerebellum and hippocampus. Withania somnifera (WS) improve rotenone induced damage in cerebellum.Aim of the work: To study the effects of oral administration of TiO2 nanoparticles on the cerebellum and the protective role of hydromethanolic root extract of WS (Egyptian Ashwaghandha).Material and Methods: Seventy five adult male albino mice were divided into five groups (N=15): group 1 (control), group 2 (gum acacia group), and group 3 (WS root extract group) recieved orally hydromethanolic WS root extract (500 mg/kg) once daily, group 4 (TiO2 nanoparticles group) recieved orally TiO2 nanoparticles (150 mg/ Kg), dissolved in gum acacia solution once daily, group 5 (TiO2 nanoparticles + WS root extract group). After sixty days, Sections from the cerebellum were prepared and stained with HandE, Cresyl violet stain as well as Immunohistological stains for nNOS, iNOS, eNOS and GFAP. Morphometrical and statistical analyses were performed.Results: In TiO2 nanoparticles group, cerebellum showed disrupted purkinje cells with marked degenerative changes, decreased Nissl granules in Purkinje cells, vacuolations in all layers of the cerebellar cortex and dilated congested capillaries in white matter. The number of the Purkinje cell were decreased while GFAP positive astrocytes were increased. Decreased nNOS immunostaining in cortical layers, while the iNOS immunostaining increased and increased eNOS immunostaining in capillary endothelial cells were detected. Administration of the hydromethanolic WS root extract improved the altered cerebellar morphology with significant statistical improvement in purkinje cell and astrocyte count.Conclusion: TiO2 nanoparticles oral administration induced toxic effects and WS contains active ingredients that counteract these effects.

Ameliorating effects of dietary mixture of Withania somnifera root extract and vitamin C in Labeo rohita against low pH and waterborne iron stresses

The present study was conducted to elucidate the possible effect of a combination of Withania somnifera (Ashwagandha) root extract and dietary l-ascorbic acid (vitamin C) on the immune responses and survivability in Labeo rohita fingerlings against low pH and waterborne iron toxicity. Four diets were prepared containing 0% (control), 0.01% (T1), 0.1% (T2) and 1.0% (T3) of a combination of Withania somnifera (Ashwagandha) root extract and dietary l-ascorbic acid (vitamin C) at the rate of 1:1 (w/w). Fishes were fed for 60 days to find out the best dose and duration of feeding by examining different haematological (haemoglobin, haematocrit, total RBC count, total WBC count, MCV, MCH and MCHC), immunological (NBT, lysozyme and total immunoglobulin activity) and biochemical (glucose, total protein, ALP, SGPT and SGOT level) parameters on 7, 15, 30 and 60 days of feeding. The best response was observed in fish fed with diet T3 within 15 days. The result showed a significant (p < 0.05) increase in haemoglobin, haematocrit, total RBC count, total WBC count, NBT, lysozyme activity, total immunoglobulin and total protein whereas a significant (p < 0.05) decrease in glucose, ALP, SGPT and SGOT level compared to control. Challenged study against waterborne iron (6.02 mg l−1) and low pH (4.2–4.5) after feeding the fish for 15 days with diet T3 showed significantly (P < 0.05) higher protection than control. The study concludes that inclusion of 1.0% Withania somnifera (Ashwagandha) root extract and dietary l-ascorbic acid (vitamin C) combination in diet have a stimulatory effect on immune response and reduces the effect of multiple stresses (i.e., low pH and waterborne iron toxicity) in L. rohita fingerlings.

Nitric oxide induced by Indian ginseng root extract inhibits Infectious Bursal Disease virus in chicken embryo fibroblasts in vitro

Infectious Bursal Disease is a severe viral disease of chicken responsible for serious economic losses to poultry farmers. The causative agent, Infectious Bursal Disease virus, is inhibited by nitric oxide. Root extract of the Indian ginseng, Withania somnifera, inhibits Infectious Bursal Disease virus in vitro. Also, Withania somnifera root extract is known to induce nitric oxide production in vitro. Therefore, the present study was undertaken to determine if the inhibitory activity of Withania somnifera against Infectious Bursal Disease virus was based on the production of nitric oxide. We show that besides other mechanisms, the inhibition of Infectious Bursal Disease virus by Withania somnifera involves the production of nitric oxide. Our results also highlight the paradoxical role of nitric oxide in the pathogenesis of Infectious Bursal Disease.

Antihyperalgesic effects of ashwagandha (Withania somnifera root extract) in rat models of postoperative and neuropathic pain.

The root of Withania somnifera, commonly known as ashwagandha, is a traditional herb in the Indian Ayurvedic system of medicine and is used as a tonic. Here, we investigated whether W. somnifera root extract exhibits analgesic effects in plantar incision (PI) and spared nerve injury (SNI) rat models. Mechanical withdrawal threshold (MWT) was measured by von Frey filaments, and pain-related behavior was determined after operation by ultrasonic vocalization (USV) measurements. Indeed, we examined interferon-γ (IFN-γ) and interleukin-10 (IL-10) levels in the isolated dorsal root ganglia (DRG) following SNI in rats using an ELISA cytokine assay. MWT significantly increased 6 and 24h after PI in rats receiving W. somnifera root extracts (100 and 300mg/kg). Furthermore, the number of 22-27-kHz USV, which are a distress response, was significantly reduced at 6 and 24h after PI in W. somnifera-treated rats (100 and 300mg/kg). SNI-induced hyperalgesia and cytokine levels were significantly alleviated after treating with W. somnifera root extracts (100 and 300mg/kg) for 15 continuous days. The main active compound, withaferin A, from the W. somnifera root extract has shown the CC chemokine family Receptor 2 (CCR2) antagonistic effects on monocyte chemoattractant protein-1 (MCP-1)-induced Ca2+ response in CCR2 stable cell line. These results indicate that W. somnifera root extract has a potential analgesic effect in rat models for both postoperative and neuropathic pain and shows potential as a drug or supplement for the treatment of pain.

LC-MS, GC-MS, and NMR Spectroscopy Based Evaluation of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract

Withania somnifera root extract which contains withanolides as major active constituent and it is used traditionally for the prevention and treatment of several diseases. The objective of the current study was to investigate the impact of The Trivedi Effect® - Consciousness Energy Healing Treatment on the structural properties of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while other part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time of the phytoconstituents remained same in the control and Biofield Energy Treated samples, whereas the peak area% i.e. the relative amount of the phytoconstituents at respective retention time was significantly altered. The peak area% at Rt of 5.6, 6.8, 6.9, 7.2, 7.9, 8.4, 8.5, 8.6, and 9.2 minutes of the treated sample were increased significantly in the range of 1.46% to 253.06% compared to the control sample. In the contrary, the peak area% of the treated sample at Rt of 6.4 and 8.2 minutes were significantly decreased by 12.72% and 17.35%, respectively with respect to the control sample. A total of 16 withanolides such as withanoside IV, coagulin Q, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A, withanolide sulfoxide, withanolide B, etc. were proposed with their structure from the molecular mass at m/z 783, 621, 489, 473, 767, 471, 505, and 992 at retention times of 6.4, 6.8, 7.2, 7.9, 8.2, 8.4, and 9.1 minutes, respectively with the help of LC-MS, GC-MS and NMR data of both the control and Biofield Energy Treated samples. The mass peak intensities of the Biofield Energy Treated sample were significantly changed in the range of -81.36% to 1720.90% compared with the control sample at the same retention time. These findings suggest that The Trivedi Effect® - Consciousness Energy Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of W. somnifera extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging, and other chronic infections.

Consciousness Energy Healing Treatment on Withania somnifera Root Extract and Its Effects on the Physical, Spectroscopic, Thermal and Behavioral Properties

Consciousness Energy Healing Treatment on Withania somnifera Root Extract and Its Effects on the Physical, Spectroscopic, Thermal and Behavioral Properties

Consciousness Energy Healing Treatment on Withania somnifera Root Extract and Its Effects on the Physical, Spectroscopic, Thermal and Behavioral Properties

Consciousness Energy Healing Treatment on Withania somnifera Root Extract and Its Effects on the Physical, Spectroscopic, Thermal and Behavioral Properties

Consciousness Energy Healing Treatment on Withania somnifera Root Extract and Its Effects on the Physical, Spectroscopic, Thermal and Behavioral Properties

Consciousness Energy Healing Treatment on Withania somnifera Root Extract and Its Effects on the Physical, Spectroscopic, Thermal and Behavioral Properties

Consciousness Energy Healing Treatment on Withania somnifera Root Extract and Its Effects on the Physical, Spectroscopic, Thermal and Behavioral Properties

Consciousness Energy Healing Treatment on Withania somnifera Root Extract and Its Effects on the Physical, Spectroscopic, Thermal and Behavioral Properties

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract

LC-MS, GC-MS, and NMR Spectroscopy Based Evaluation of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract

LC-MS, GC-MS, and NMR Spectroscopy Based Evaluation of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract

LC-MS, GC-MS, and NMR Spectroscopy Based Evaluation of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract

LC-MS, GC-MS, and NMR Spectroscopy Based Evaluation of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract

LC-MS, GC-MS, and NMR Spectroscopy Based Evaluation of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract

LC-MS, GC-MS, and NMR Spectroscopy Based Evaluation of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract

Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy

Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy