Winter Type Research Articles

Winter circulation weather types and hospital admissions for cardiovascular, respiratory and digestive diseases in Salamanca, Spain

AbstractThe aim of this study is to evaluate the possible associations between daily hospital admissions for cardiovascular, respiratory and digestive diseases in Salamanca (Spain) and weather conditions during the winters of 1995–2005. An objective daily air mass classification was performed by means of statistical analysis of ground‐level meteorological data. This afforded seven main circulation weather types (CWTs) for the cold period, and the frequency distributions of these types and their meteorological characteristics were analysed.A summary of the main characteristics of the hospital admission series and their distribution over the 10 years studied, together with the frequency distributions of the different diseases classified by ages, the average number of the hospital admissions for each day of week, type of disease and sex, is given. In addition, a comparison between air mass classification and hospital admissions was made using an admission index (AI), for each disease. Significant increases in hospital admissions for the cardiovascular AI were evident 24–48 h after a day characterized by an anticyclonic air mass, 4–5 days after a day characterized by a westerly air mass and 6–7 days after an easterly flow. For the respiratory AI, an increase was observed 2–3 days after a cyclonic situation, 4 days after a northeasterly situation, while for days characterized by southwesterly air masses no delay was observed. These results indicate that the effects of weather conditions on hospital admissions tend to occur some days after exposure. Copyright © 2008 Royal Meteorological Society

Selection of winter types of wheat from its spring types sawn in winter after prior vernalization

In the Mironivka Institute for wheat named by V.M. Remeslo UAAN new winter-hardy winter wheat variety material was obtained. The annual breeding was used for the selection df winter wheat forms from spring wheat varieties by the prevernalization, spring sowing and M2 at the beginning of optimal terms in autumn (the influence of low temperature on spring wheat seeds). On the basis of such material, winter-hardy wheat varieties were created, such as Myronivs’ka rannyostygla, Remeslivna, Vo- loshkova and such varieties as Bagira, Vdyachna and Svyankova were submitted to the State Service for Plant Variety Protection.

Tocopherols and Tocotrienols in Wheat Genotypes in the HEALTHGRAIN Diversity Screen

Tocopherol and tocotrienol compositions were studied in 175 genotypes of different wheat types grown under similar conditions to screen for natural diversity. The main focus was on bread wheats, including 130 and 20 winter and spring types, respectively. The average total content of tocopherols and tocotrienols was 49.4 microg/g of dm, with a range of 27.6-79.7 microg/g of dm, indicating a 2.9-fold variation among genotypes. Beta-tocotrienol and alpha-tocopherol were the major vitamers, and in general there were more tocotrienols than tocopherols. In the early cultivated forms of wheat the proportion of tocotrienols was especially high, at >or=62.5%. In conclusion, there was a large variation in total tocopherol and tocotrienol contents in bread wheats and this, along with the high proportions of tocotrienols in other types of wheat, demonstrates the great genetic potential of genotypes to be exploited by plant breeders.

Variation in agronomic, rust reaction and grain quality characteristics in a collection of winter and facultative wheat varieties

A collection of 110 wheat varieties from different agroecological zones in Central Asia and the Caucasus (CAC), Russia, and Ukraine were evaluated for growth habit (winter, facultative, spring), growth type (prostrate versus erect), days to heading, resistance to yellow rust and leaf rust, and quality traits. The study was conducted at the experimental farms of International Center for Agricultural Research in the Dry Areas (ICARDA) at Terbol in Lebanon and Tel Hadya in Syria to identify promising rust resistant wheat cultivars and lines that can be used in crossing program by cooperative Turkey-CIMMYT-ICARDA winter wheat breeding program. The study also aimed to assess the distribution of winter versus facultative versus spring types in the regional gene pool. Higher frequencies of winter types occurred in germplasm from Ukraine, Russia, southern Kazakhstan, Armenia, Uzbekistan and Georgia, whereas the frequencies of spring wheat in Azerbaijan and Turkmenistan were 33% and 20%, respectively. There were significant correlations between growth type, and both growth habit and cold tolerance (r = 0.600*** and 0.57**). This indicates that winter wheat varieties tend to be prostrate and cold tolerant, whereas facultative and spring wheats have are more erect and cold sensitive. Among the tested material 60% of varieties were resistant to yellow rust and 44%—to leaf rust. More than 20 genotypes demonstrated resistance to both rusts and useful variation for other traits proving good parents for crosses.

Yr10 gene polymorphism in bread wheat varieties

Yellow rust resistance locus Yr10 located on chromosome 1B in Moro and originated from the Turkish line PI178383 was investigated in terms of polymorphism in seven winter type bread wheat cvs. (Triticum aestivum ssp. Aestivum) Altay2000, zgi2001, Sonmez2001 (yellow rust resistant), Aytýn98, ES14, Harmankaya99 (yellow rust susceptible) and PI178383 as control. Exon 1 (1 - 833 bp) and Exon 2 (1989 - 3630 bp) parts of Yr10 were amplified with three primers. Amplification was not observed with E2A primers in Harmankaya99, zgi2001 and Sonmez2001 cvs, while amplification products were observable at all tested varieties with the other primers. PCR results showed that E2A reverse primer is not able to anneal to the three varieties mentioned above. Sequence analysis and bioinformatics analysis proved that there has been single nucleotide changes especially in the second exon. The most similar sequences to the first exon of Harmankaya99, zgi01 and Sonmez2001 are AF509535 (Aegilops tauschii NBS-LRR-like gene), AF509534 (A. tauschii NBS-LRR-like gene sequence) and AF509534, respectively. These results could be helpful in revealing divergence between resistant and susceptible varieties.

Doubled haploid production via anther culture in annual, winter type of caraway (Carum carvi L.)

Caraway (Carum carvi L.) is a traditional medicinal and spice cross-pollinated plant species. Although in vitro techniques are recently extensively applied in plant breeding programmes, these are not commonly utilized in caraway. Therefore, based on the protocol for anther culture in carrot (Daucus carota L., a closely related species of caraway in Daucaceae family), in vitro androgenesis in caraway has been studied with the aim to produce completely homozygous inbred lines. Various induction conditions, such as temperature pretreatments, carbon sources and combination of growth regulators in a culture medium as well as the effect of genotype on in vitro androgenesis were examined. Ten breeding lines of winter caraway representing third generation of forced (artificial) self-pollination were used as donor plant material. Cultured anthers produced embryogenic calli, and subsequently two types of regenerated plants were obtained, namely haploids with evident microspore origin, and diploids which may represent somatic (anther wall) regenerants or spontaneous doubled haploids. The ploidy status of regenerated plants was determined by flow cytometry. This is the first report on androgenic doubled haploid production in caraway.

Adaptation strategy, germplasm type and adaptive traits for field pea improvement in Italy based on variety responses across climatically contrasting environments

A pea breeding strategy is required to cope with the large climatic variation featuring south-European environments. Thirty-seven recent cultivars bred by 21 European or Australian institutions were grown in two climatically contrasting Italian sites (Lodi, subcontinental; Foggia, Mediterranean), two cropping years per site and two sowing times per year, to define various elements of this strategy. The study assessed: (i) the impact of genotype × environment (GE) interaction due to spatial and temporal factors on the consistency of top-yielding cultivars; (ii) the similarity between environments for GE effects and its implications on adaptation strategies; (iii) the extent of genotypic and GE interaction effects, and the relationship with adaptive responses, for various morphophysiological traits; (iv) the adaptation pattern and the combination of adaptive traits featuring three germplasm types, i.e. European spring and winter types, and germplasm selected in Mediterranean environments; (v) the predicted efficiency of direct and indirect selection procedures for grain yield. The geoclimatic area had a major impact on crop yield (5.15 t/ha in Lodi vs. 2.52 t/ha in Foggia) but tended to affect GE interaction less than time or year of autumn sowing, suggesting to breed for wide adaptation. Top-yielding cultivars as modeled by additive main effects and multiplicative interaction were environment-specific. On average, spring and winter materials outyielded the Mediterranean germplasm but the spring type, characterized by wide entry variation, included most widely- and specifically-adapted top-yielding cultivars. Cold-tolerant spring-type germplasm is preferable to breed for wide adaptation as it may combine high yield potential with adaptation to winter cold and terminal drought and heat stress. Lodging susceptibility, harvest index, onset and duration of flowering, and canopy height at maturity assessed in individual environments showed moderate to fairly high broad-sense heritability on a plot basis ( h 2 > 0.20) and tended to correlation with yield over test environments ( r ≥ 0.20). An indirect selection index including harvest index and canopy height exhibited about 20% greater predicted efficiency than direct selection for yield when using one selection environment and could be preferred for early selection stages. Direct yield selection in late selection stages should ideally be performed across 2 years in two environments that contrast for geoclimatic area and time of autumn sowing.

퍼스널 컬러 유형과 성격 유형과 상관성 및 색상 선호도

The purpose of this study is to find out the relationship between the personal color types and personality types and analyze the differences in color preference according to the personal color types and personality types. This study was conducted by using questionnaire method and drape diagnosis method in parallel. The results are as follows. First, in case of introversion (I), the summer type took the most and spring, fall, and winter type followed respectively. Also in the case of extroversion (E), the summer type took the most and spring, fall, and winter type followed respectively. Second, the spring type preferred yellow, the summer type, blue, the fall type, brown, and winter type, blue the most. For the preference of the tone of color, all 4 types preferred the pastel tones the most. For the lipstick colors, the spring and summer type preferred the pink color, the fall type, orange and winter type, red the most. For eye shadows, the spring type preferred orange, summer type, blue, fall type, orange and winter type, pink. For nail colors, all 4 types preferred pink the most. Third, the most preferred color for introversion (I) was red group. In contrast, the most preferred color for extroversion (E) was yellow group. In the tone of preferred colors, both introversion and extroversion preferred the pastel tones the most. In lipstick colors, eye shadow colors, and nail colors, both introversion and extroversion preferred the pink group colors the most.

Identification of SSR and RAPD markers associated with QTLs of winter survival and related traits in Brassica napus L.

Because of importance of winter survival in winter type of Brassica napus, this study was performed to identify the QTLs controlling winter survival and related traits using SSR and RAPD markers. For this, an F2:3 population of 200 families derived from crossing between cv. ‘SLMO46’ (winter type and cold resistant) and cv. ‘Quantum’ (spring type and susceptible to low temperature) were used. Winter survival (WS), leaves per plant in rosette stage (L/P), crown wet weight (CWW), crown dry weight (CDW) and crown water content (CWC) were measured in F3 families. 350 SSR primer pairs and 250 RAPD primers were used to assess the parental polymorphism. The 32 SSR primer pairs and 47 RAPD polymorphic markers between parental lines were used to screen F2 individuals. Linkage map was constructed using polymorphic markers. The markers were assigned into 14 linkage groups with total length of 1199.1 cM and an average distance of 17.13 cM between adjacent markers. The relationship between measured traits and genotypic data was analyzed using CIM method and totally 12 putative QTLs were detected for studied traits. The explained phenotypic variance by identified QTLs ranged between 0.5 and 11%. The identified QTLs had positive and negative additive effects and transferred from both parents to F2 plants and F3 families. Some of these QTLs located in the same genomic regions.

Allelic Variation at the Vernalization Genes Vrn‐A1, Vrn‐B1, Vrn‐D1, and Vrn‐B3 in Chinese Wheat Cultivars and Their Association with Growth Habit

Information on the distribution of vernalization genes and their association with growth habit is crucial to understanding the adaptability of wheat (Triticum aestivum L.) cultivars to different environments. In this study, 278 Chinese wheat cultivars were characterized with molecular markers for the vernalization genes Vrn‐A1, ‐B1, ‐D1, and ‐B3 Heading time was evaluated in a greenhouse under long days without vernalizaton. The dominant Vrn‐D1 allele showed the highest frequency in the Chinese wheat cultivars (37.8%), followed by the dominant Vrn‐A1, ‐B1, and ‐B3 alleles. Ninety‐two winter cultivars carried recessive alleles of all four vernalization loci, whereas 172 spring genotypes contained at least one dominant Vrn allele. All cultivars released in the North China Plain Winter Wheat Zone were winter type. Winter (53.0%), spring (36.1%), and early‐heading (10.9%) cultivars were grown in the Yellow and Huai River Valley Winter Zone. Most of the spring genotypes from this zone carried only the dominant Vrn‐D1 allele, which was also predominant (64.1%) in the Middle and Lower Yangtze Valley Winter Zone and Southwestern Winter Wheat Zone. In three spring‐sown wheat zones, all cultivars were early‐heading spring types that frequently possessed the strongest dominant Vrn‐A1a allele and combinations with other dominant Vrn gene(s). The Vrn‐D1 allele is associated with the latest heading time, Vrn‐A1 the earliest, and Vrn‐B1 intermediate values. The information is important for breeding programs in countries interested in using Chinese wheats.

Analysis of Quantitative Trait Loci Associated with Freezing Tolerance in Rapeseed (Brassica Napus L.)

ABSTRACTFreezing tolerance is a major component of winter survival and is the ability of plant to survive subfreezing temperatures. In order to identify the molecular markers linked to chromosomal regions governing freezing tolerance in rapeseed, an F2:3 population of 199 plants derived from crossing between cv. ‘SLMO46’ (winter type and cold resistant) and cv. ‘Quantum’ (spring type and susceptible to low temperature) were used. The LT50 (the temperature at which 50% of plants were killed) was measured as a cold resistance index in F3 families. The parental polymorphism was assessed using 350 SSR primer pairs and 250 RAPD primers. The 32 microsatellite and 47 RAPD markers, polymorphic between parental lines were used to screen F2 individuals. Linkage map was constructed using polymorphic markers. The markers were assigned into 14 linkage groups with total length of 1199.1 cM and an average distance of 17.13 cM between adjacent markers. The relationship between LT50 and genotypic data was analyzed using single marker analysis (SMA), interval mapping (IM) and composite interval mapping (CIM) methods and four putative QTLs were detected. These QTLs explained 24% of the LT50 total phenotypic variance.

Quantitative Trait Loci in Bread Wheat (Triticum Aestivum L.) Associated with Resistance to Stripe Rust

ABSTRACTStripe rust is an important wheat disease worldwide and resistance to stripe rust is often not controlled by race-specific resistance genes. To identify quantitative trait loci (QTL) affecting quantitative resistance to stripe rust, 329 F2:3 families from a cross between highly resistant winter type cv. MV17 and highly susceptible spring type local cv. Bolani were used. The population was evaluated for adult plant resistance to pathotype 134E134A+ of Puccinia striiformis, in the field condition and area under the disease progress curve (AUDPC) was calculated. Fifty six polymorphic SSR markers between parental genotypes were screened in the population and linkage map was constructed using these markers. Based on composite interval mapping (CIM) 14 regions on nine (4A, 1B, 2B, 3B, 4B, 5B, 6B, 2D and 7D) out of the 21 wheat chromosomes were significantly associated with AUDPC. The effect of QTLs varied from 0.01 to 8.00% giving an aggregate effect of 34.3% for AUDPC. Leaf tip necrosis (LTN) was also recorded as presence and absence and the phenotype was used as a dominant Mendelian morphologic marker. Linkage analysis revealed association between LTN and Xbarc352 SSR marker on chromosome 7D.

Tensile Test of Cabbage Leaves for Quality Evaluation of Shredded Cabbage

The mechanical properties of six cabbage varieties harvestable in May in Miura Peninsula, Japan, were measured by subjecting strap-shaped specimens to tensile testing. The fracture properties greatly differed with direction of veins and cultivars. No single parameter characterized the physical properties of cabbage varieties. The mechanical variables in tensile tests conducted parallel and perpendicular to the veins were subjected the principal component analysis. The six cultivars were evidently categorized into three groups. Intermediate varieties were separated into two; one resembled to the Winter type, which is suitable for shredded cabbage, and the other was similar to the Spring type.

Study of faba bean ( Vicia faba L.) winter-hardiness and development of screening methods

In cool-temperate regions, faba bean ( Vicia faba L.) is mainly grown as a spring crop despite the higher yield potential of the winter type, because of the insufficient winter-hardiness of the present winter genotypes. The objective of this study was to assess winter-hardiness and frost tolerance, to quantify the hardening effect on physiological traits, and to identify auxiliary traits for winter-hardiness. To do so, 31 representative entries were tested in controlled frost tests for frost tolerance and in 12 European environments for winter-hardiness. Total fatty acid composition, proline content, and electrolyte leakage of leaves were analysed. Across all environments, five European winter genotypes were identified with superior winter-hardiness. Controlled frost tests indicated that frost tolerance is a significant, but not an exhaustive component of winter-hardiness (0.021 < | r| < 0.737**). These tests revealed the high frost tolerance of several poorly winter-hardy experimental lines and the limited frost tolerance of well-known winter types. Fatty acid changes due to hardening, proline content, and electrolyte leakage were more strongly correlated with frost tolerance than with field-based winter-hardiness. Although frost tolerance, fatty acid composition, proline content, and electrolyte leakage were significantly correlated with winter-hardiness, the rather low correlation values do not allow a general use of one of them alone to indirectly select for winter-hardiness.

Insights and Innovations from Wide Crosses: Examples from Canola and Tomato

ABSTRACTWild relatives and unadapted forms of crop plants are important reservoirs of allelic variation that can be accessed through wide crosses for improvement of crop plants. In this paper, we describe insights and innovations from the use of wide crosses to transfer favorable alleles from wild or unadapted forms of two crops: canola (oilseed Brassica napus L.) and tomato (Lycopersicon esculentum Mill.). The canola research involves the use of unadapted forms (winter types and resynthesized B. napus) in wide crosses to spring‐seeded types to increase seed yield of hybrids. The tomato research involves the use of wild tomato relatives to improve several traits, including disease resistance and fruit quality. These two crop plants provide a broad range of insights and innovations that have been gained from wide crosses in plant breeding research, including the impact of chromosomal rearrangements on allelic variation among unadapted forms of B. napus, and innovations that can be achieved from stacking elite alleles in tomato. The examples presented in this paper highlight the value of using DNA markers for understanding and applying the results of wide crosses.

Hydrologic Model Framework for Water Resources Planning in the Santa Cruz River, Southern Arizona1

Abstract: The authors develop a model framework that includes a set of hydrologic modules as a water resources management and planning tool for the upper Santa Cruz River near the Mexican border, Southern Arizona. The modules consist of: (1) stochastic generation of hourly precipitation scenarios that maintain the characteristics and variability of a 45‐year hourly precipitation record from a nearby rain gauge; (2) conceptual transformation of generated precipitation into daily streamflow using varied infiltration rates and estimates of the basin antecedent moisture conditions; and (3) surface‐water to ground‐water interaction for four downstream microbasins that accounts for alluvial ground‐water recharge, and ET and pumping losses. To maintain the large inter‐annual variability of streamflow as prevails in Southern Arizona, the model framework is constructed to produce three types of seasonal winter and summer categories of streamflow (i.e., wet, medium, or dry). Long‐term (i.e., 100 years) realizations (ensembles) are generated by the above described model framework that reflects two different regimes of inter annual variability. The first regime is that of the historic streamflow gauge record. The second regime is that of the tree ring reconstructed precipitation, which was derived for the study location. Generated flow ensembles for these two regimes are used to evaluate the risk that the regional four ground‐water microbasins decline below a preset storage threshold under different operational water utilization scenarios.

A Synoptic Climatology of the Central United States and Associations with Pacific Teleconnection Pattern Frequency

Abstract A synoptic climatological weather classification scheme incorporating both surface and upper-air data is developed for the central United States based on an automated two-step cluster analysis. It employs daily NCEP–NCAR reanalysis data over all seasons of 57 yr (1948–2004) in creating synoptic types from surface and upper-air (925, 850, 700, and 500 hPa) temperature and humidity data as well as sea level pressure, geopotential heights, and winds aloft. The cluster analysis creates 10 synoptic types exhibiting distinct seasonal preferences, with three each that occur primarily in summer and winter, and four that occur primarily in winter and the transition seasons, particularly spring. The typing scheme generates synoptic patterns largely characterized by distinctive surface circulations, baroclinic vertical structure, and thermal advection. Interannual variations occur in the frequencies of the synoptic types, some of which are out of phase with each other. The annual frequencies of two winter synoptic types, associated respectively with strong zonal and meridional flow, are highly correlated (r ≫ 0.63–0.73) to the phase of the Pacific–North American teleconnection pattern, while Niño-3.4 equatorial Pacific sea surface temperatures are linked to a synoptic type producing low pressure around the Gulf Coast.

Identification of QTLS Controlling Winter Survival in Brassica Napus using Rapd Markers

ABSTRACTTwo hundred F2:3 families of rapeseed (Brassica napus L.) derived from crossing between cv. SLMO46 (winter type and cold resistant) and cv. Quantum (spring type and susceptible to low temperature) were used as genetic material. Winter survival of F3 plants was measured in a complete block design with two replications. The polymorphism of parental lines was assessed using RAPD method and 47 RAPD markers, polymorphic between parental lines were used to screen F2 individuals. By using of the polymorphic markers linkage map was constructed as the markers were assigned into 9 linkage groups with total length of 860.8 cM and an average distance of 20.49 cM between adjacent markers. The relationship between winter survival and genotypic data was analyzed using composite interval mapping (CIM) method and one putative QTL was detected that explained five percents of phenotypic variances of this trait. This QTL is located on 6th linkage group and had negative additive effect.

Validation of the VRN-H2/VRN-H1 epistatic model in barley reveals that intron length variation in VRN-H1 may account for a continuum of vernalization sensitivity

The epistatic interaction of alleles at the VRN-H1 and VRN-H2 loci determines vernalization sensitivity in barley. To validate the current molecular model for the two-locus epistasis, we crossed homozygous vernalization-insensitive plants harboring a predicted "winter type" allele at either VRN-H1 (Dicktoo) or VRN-H2 (Oregon Wolfe Barley Dominant), or at both VRN-H (Calicuchima-sib) loci and measured the flowering time of unvernalized F(2) progeny under long-day photoperiod. We assessed whether the spring growth habit of Calicuchima-sib is an exception to the two-locus epistatic model or contains novel "spring" alleles at VRN-H1 (HvBM5A) and/or VRN-H2 (ZCCT-H) by determining allele sequence variants at these loci and their effects relative to growth habit. We found that (a) progeny with predicted "winter type" alleles at both VRN-H1 and VRN-H2 alleles exhibited an extremely delayed flowering (i.e. vernalization-sensitive) phenotype in two out of the three F(2) populations, (b) sequence flanking the vernalization critical region of HvBM5A intron 1 likely influences degree of vernalization sensitivity, (c) a winter habit is retained when ZCCT-Ha has been deleted, and (d) the ZCCT-H genes have higher levels of allelic polymorphism than other winterhardiness regulatory genes. Our results validate the model explaining the epistatic interaction of VRN-H2 and VRN-H1 under long-day conditions, demonstrate recovery of vernalization-sensitive progeny from crosses of vernalization-insensitive genotypes, show that intron length variation in VRN-H1 may account for a continuum of vernalization sensitivity, and provide molecular markers that are accurate predictors of "winter vs spring type" alleles at the VRN-H loci.

Characterization of flowering time and SSR marker analysis of spring and winter type Brassica napus L. germplasm

Flowering dates and life forms of all available Brassica napus accessions conserved at the North Central Regional Plant Introduction Station (NCRPIS) were characterized, and a survey of molecular variation was conducted by using simple sequence repeats (SSR) in order to support better management of accessions with diverse life forms. To characterize flowering phenology, 598 B. napus accessions from the NCRPIS collection were planted in Iowa and Kansas field sites together with a current commercial cultivar and observed for days to flowering (first, 50% and 100% flowering) in 2003. Days from planting to 50% flowering ranged from 34 to 83 in Iowa and from 53 to 89 in Kansas. The mean accumulated growing degree days (GDD) to 50% flowering were 1,997 in Iowa, and 2,106 in Kansas. Between locations, the correlation in flowering time (r = 0.42) and the correlation in computed GDD (r = 0.40) were both significant. Differences in flowering-time rank were observed for several accessions. Accessions that failed to flower in Iowa in a single growing season comprised 28.5% of the accessions; of the flowering accessions, 100% plant flowering was not always achieved. Accessions were grouped according to flowering time. A stratified sample of 50 accessions was selected from these groups, including 10 non-flowering and 40 flowering accessions of diverse geographic origins and phenological variation. The flowering time observed in the sampled accessions when grown in the greenhouse were found to be significantly correlated to the flowering time observed in the field locations in Iowa (r = 0.79) and Kansas (r = 0.49). Thirty SSR markers, selected across 18 Brassica linkage groups from BrassicaDB, and 3 derived from Brassica expressed sequence tags (ESTs) were scored in the stratified sample. An average of three bands per SSR primer pair was observed. Associations of SSR marker fragments with the life forms were determined. Analysis of molecular variation by using cluster analysis and ordination resulted in recognizable, distinct groups of annual and biennial life-form types, which may have direct applications for planning and management of future seed regenerations.