Winter Symposium Research Articles

The high‐risk recipient: the Eighth Annual American Society of Transplant Surgeons’ State‐of‐the‐Art Winter Symposium

The evolution of organ transplantation has produced results so successful that many transplant programs commonly see recipients with medical risks, which in the past, would have prohibited transplantation. The Eighth Annual American Society of Transplant Surgeons State-of-the-Art Winter Symposium focused on the high-risk recipient. The assessment of risk has evolved over time, as transplantation has matured. The acceptance of risk associated with a given candidate today is often made in consideration of the relative value of the organ to other candidates, the regulatory environment, and philosophical notions of utility, equity, and fairness. In addition, transplant programs must balance outcomes, transplant volume, and the costs of organ transplantation, which are impacted by high-risk recipients. Discussion focused on various types of high-risk recipients, such as those with coronary artery disease, morbid obesity, and hepatitis C; strategies to reduce risk, such as down-staging of hepatocellular carcinoma and treatment of pulmonary hypertension; the development of alternatives to transplantation; and the degree to which risk can or should be used to define candidate selection. These approaches can modify the impact of recipient risk on transplant outcomes and permit transplantation to be applied successfully to a greater variety of patients.

Implementation Challenges for Clinical and Research Information Systems: Recommendations from the 2007 Winter Symposium of the American College of Medical Informatics

The American College of Medical Informatics (ACMI), is “a college of elected fellows from the United States and abroad who have made significant and sustained contributions to the field of medical informatics” and is a component of the American Medical Informatics Association (AMIA). Over the last few years in its winter symposium, ACMI has discussed a variety of issues related to the adoption and deployment of electronic health records (EHRs). Beginning in winter 2004, ACMI addressed what would be needed to promote greater adoption of electronic health records. The recommendations led to AMIA's “Got EHR?” initiative (www.amia.org/gotehr) and a series of articles summarizing the ACMI participants' recommendations.1–4 In 2005, the ACMI symposium focused on personal health records (PHRs), and the resulting article outlined recommendations for the deployment of PHRs, an area that is now showing increasing development nationwide.5 In 2006, with the growing interest in clinical decision support (see AMIA's Clinical Decision Support Roadmap initiative http://www.amia.org/inside/initiatives/cds/), ACMI addressed the knowledge management that would be needed for effective broad-based use of clinical decision support systems. These recommendations were presented at the 2006 AHRQ Patient Safety and Healthcare …

Formative Evaluation: A Critical Component in EHR Implementation

This Viewpoint paper has grown out of a presentation at the American College of Medical Informatics 2007 Winter Symposium, the resulting discussion, and several activities that have coalesced around an issue that most informaticians accept as true but is not commonly considered during the implementation of Electronic Health Records (EHR) outside of academia or research institutions. Successful EHR implementation is facilitated and sometimes determined by formative evaluation, usually focusing on process rather than outcomes. With greater federal funding for the implementation of electronic health record systems in health care organizations unfamiliar with research protocols, the need for formative evaluation assistance is growing. Such assistance, in the form of tools and protocols necessary to do formative evaluation and resulting in successful EHR implementations, should be provided by practicing medical informaticians.

Solving the Organ Shortage Crisis: The 7th Annual American Society of Transplant Surgeons’ State-of-the-Art Winter Symposium

The 2007 American Society of Transplant Surgeons' (ASTS) State-of-the-Art Winter Symposium entitled, 'Solving the Organ Shortage Crisis' explored ways to increase the supply of donor organs to meet the challenge of increasing waiting lists and deaths while awaiting transplantation. While the increasing use of organs previously considered marginal, such as those from expanded criteria donors (ECD) or donors after cardiac death (DCD) has increased the number of transplants from deceased donors, these transplants are often associated with inferior outcomes and higher costs. The need remains for innovative ways to increase both deceased and living donor transplants. In addition to increasing ECD and DCD utilization, increasing use of deceased donors with certain types of infections such as Hepatitis B and C, and increasing use of living donor liver, lung and intestinal transplants may also augment the organ supply. The extent by which donors may be offered incentives for donation, and the practical, ethical and legal implications of compensating organ donors were also debated. The expanded use of nonstandard organs raises potential ethical considerations about appropriate recipient selection, informed consent and concerns that the current regulatory environment discourages and penalizes these efforts.

A Novel Major Groove Binding Site in B-form DNA for Ethidium Cation

A stably-bound external binding site for ethidium cation in the major groove of B-form DNA is proposed. This complex is stabilized by hydrogen bonding between this ligand and the nu- cleophilic centers O6 and N7 of guanine, both of which are accessible via the major groove. This binding site is not the same as the well-characterized electrostatically-stabilized external binding site, but rather is seen to be a covalently bound complex which is stabilized by two hydrogen bonds between the ethidium ligand and guanine in the double stranded (ds) B-form DNA. This site [(1), R. Monaco, F. Hasheer. J Biomol Struct Dyn 10, 675 (1993)] can only exist at very low occupancy ratios. The existence of this binding site leads directly to the expectation that there will exist particular mechanistic steps along the pathway of interaction between ethidium and ds B-DNA at low and high ligand concentrations that involve this binding mode. This would not only explain observations published recently [for example, see (2–6), W. Wilson, I. Lopp. Biopolymers 18, 3025 (1979); L. Wakelin, M. Waring. J Mol Biol 144, 183–214 (1980); A. Karpetyan, N. Mehrabian, G. Terzikian, A. Antonian, P Vardevanian, M. Frank-Kamenetshii. Proceedings of the 10th Conversation, SUNY Albany, 275 (1998); P Vardevanyan, A. Antonyan, G. Manukyan, A. Karapetyan. Experimental and Molecular Medicine 33, 205 (2001); P Vardevanyan, A. Antonyan, L. Minasbekan, A. Karapetyan. Proceedings of the 2002 Miami Nature Biotechnology Winter Symposium, 2(S1), 144 (2002)] but also give insight into discrepancies reported in the literature over the years by different workers studying the mechanism of interaction between ethidium and DNA. In this paper this novel binding interaction is discussed, and it is shown how the elucidation of this interaction led to the proposal of two distinct mechanisms of intercalation between ds B-DNA and ethidium cation for high and low concentrations of ligand. Modeling studies show the stability, configuration, and relative energies of this outside binding site. It is expected that this externally bound complex between ethidium cation and ds B-form DNA will be experimentally detectable using fluorescent polarization and/or linear and circular dichro- ism spectroscopic studies [(7, 8) E. Tuite, U. Sehlstedt, P. Hagmar, B. Nordén, M. Takahashi. Euro J Biochem 243, 482–492 (1997); T. Hard. Biopolymers 26, 613–618 (1987)].

Proceedings of the Fifth Circumpolar Ecosystems Conference and Workshop, Churchill, Manitoba, Canada, February 2004—introduction

During February 2004, we co-chaired the Fifth Circumpolar Ecosystems Conference and Workshop, hosted by the Churchill Northern Studies Centre (CNSC). More than 50 international participants traveled to Churchill for this meeting, which originated as part of the CNSC mandate of arctic research and education. In 1989, Peter Scott organized the first CNSC-hosted meeting under the banner ‘‘Circumpolar Ecosystems in Winter.’’ At high latitudes, winter can be the dominant season that drives many physical and biological processes. Understanding these winter processes is critical in the context of climate change, and yet few researchers are actively studying environmental processes during winter. One of the purposes of these Churchill meetings was to address the need to increase winter research. This was obvious when the mandate of the meetings had to be expanded to include results of research in seasons other than winter to fill the program. For the Fifth Circumpolar Ecosystems Conference we chose to continue the field science theme for the workshop by focusing on ‘‘snow sampling methodology and instrumentation.’’ Here participants had the opportunity to examine a wealth of traditional and newer technologies available to measure snow cover characteristics at a variety of scales. The second aspect of the meetings was the presentation and publication of a collection of scientific papers. The theme for the symposium was ‘‘Northern Margins: Changing Transition Zones in Time.’’ The range of papers for this symposium reflects the wide range of interest in the transitional zones that occur throughout the circumpolar north— forest-tundra, fast ice–pack ice, continuous-discontinuous permafrost, snowpack temperature gradients, and snowpack melt, to name a few. The three papers included here characterize the diversity of topics covered in the symposium. Two papers highlight research in the northern treeline transition zone in the Churchill area, focusing on snowpack variation in one case, and revegetation methods in the other. The third paper demonstrates the importance of latitudinal variation in environmental parameters on marine food webs. The meeting was sponsored by Campbell Scientific Canada Corporation; Parks Canada; Clayton H. Riddell Faculty of Earth, Environment and Resources, University of Manitoba; Enviro-Test Laboratories; University of Winnipeg; Brandon University; Eskimo Museum; Town of Churchill; Manitoba Conservation; Inter-Universities North (now University College of the North); and the Churchill Northern Store. We thank the sponsors and the residents of Churchill and CNSC staff for their support and cooperation in this endeavor. Plans for the Sixth Circumpolar Ecosystems in Winter Symposium will be announced following the summer of 2007 as the CNSC has embarked on a facility renewal campaign that will result in a new research center. The proposed date of February 2009 will give the CNSC a chance to showcase their new facility by highlighting results of the 2007–2008 International Polar Year.

Symposium on ‘The Anatomy of Tissue Engineering’

Symposium on ‘The Anatomy of Tissue Engineering’

Surgical Challenges in Transplantation: The Fourth Annual American Society of Transplant Surgeons' State-of-the-Art Winter Symposium

Surgical Challenges in Transplantation: The Fourth Annual American Society of Transplant Surgeons' State-of-the-Art Winter Symposium

Preface: Future approaches to replacement of organs

Preface: Future approaches to replacement of organs

Tumors and Transplantation: The 2003 Third Annual ASTS State-of-the-Art Winter Symposium

Tumors and Transplantation: The 2003 Third Annual ASTS State-of-the-Art Winter Symposium

A time of change

At the beginning of next year, Development's Editorial team will change. Jim Smith will take over as Editor in Chief.FIG1Looking back over my own fifteen years as Editor in Chief, the amount of change has been staggering. Development's short life began in 1987 when it was relaunched from the Journal of Embryology and Experimental Morphology. Since then, the field has seen the unification of many, if not all, model systems, into a framework that links evolution, development and medicine to an extent that only the most optimistic could have imagined in 1987. The first paper, in the first issue, of Development(Smith, 1987) showed that an unidentified factor in a tissue culture medium induced animal caps fromXenopus embryos to form mesoderm. It seems hard to imagine we didn't know this in 1987, because now all the major signaling molecules that are involved in this process have been identified; their cDNAs or genomic sequences have been characterized; their downstream signaling pathways are known, as are many of their target genes; their functions have been analyzed in large numbers of experimental systems; and the developmental consequences of mutations that affect these molecules have been discovered in humans.Times have also changed for an editor since the first issue ofDevelopment hit the presses. Then, manuscripts arrived in huge packages, sometimes after weeks at sea, often with the photographs, which had been patiently glued to bits of card, lying around loose and unnumbered at the bottoms of the envelopes. Letters and manuscripts were mailed out to reviewers, and the reviews then mailed back to authors, often resulting in long correspondences that took weeks (actually, this bit hasn't changed much!). The FedEx bills were enormous, as was the mess in the editorial office. Next, the FAX machine appeared, resulting in huge untidy piles of paper awaiting the arrival of the editors wherever they travelled, often to the horror of their hosts. And finally, the electronic revolution came, which has allowed manuscripts to be received, reviewed, discussed, returned,re-submitted, re-reviewed and sent for publication, without any of the parties concerned ever seeing a piece of paper.The publishing process has changed beyond recognition. The first few issues of Development were hand-set, and only later did the initial(unusable) dinosaurs of electronic typesetting appear, followed mercifully quickly by desk-top publishing. Throughout, Development has been blessed with a small group of dedicated, flexible and highly professional staff at the publishing house, who have struggled with the most outlandish editorial demands (I think the flip-art movie in the zebrafish issue marked the nadir of our relationship, and some of the discussions over page numbers have become the stuff of legends), have reacted manfully to adversity (the famous `missing 40 pages' from the second issue is a good example) and have retained their sense of humor throughout.But one thing that has not changed is the aim of the journal. At its reincarnation from JEEM, Development was established to publish interesting and important mechanistic papers on developmental biology, using molecular and genetic, as well as the more traditional experimental techniques. It still does this, which testifies to the scientific good taste of its editors and the high quality of its reviewing process. These are the essential factors that produce a good journal. My personal thanks go to Walter Gehring, Corey Goodman, Iva Greenwald, Richard Hynes, Tom Jessell, Peter Lawrence, Mike Levine, Andrew Lumsden, Doug Melton, Keith Roberts, Janet Rossant, Gerry Rubin and Ben Scheres, and a host of outstanding reviewers, for doing such a great job.It is particularly satisfying that Jim Smith has agreed to become the next Editor in Chief of Development, for two reasons. First, Jim has the honor of having authored the first paper published in Development, as well as the first front cover. Second, he has always been a staunch supporter of the journal. One of my fondest memories is of the first time I asked for a publicity stand for Development to be put up at a conference —a Miami Winter Symposium. When it arrived, late in the evening before the meeting started, it turned out to be a vast, complicated, and disassembled contraption with no instructions, and no tools. Jim dropped everything to help me spend most of the night putting the thing together, and then manned it with me for most of the meeting, pointing out vigorously to passers-by the advantages of publishing in Development. I predict that he will have just the same attitude as Editor in Chief.

Special Issue: 32nd Winter Symposium on the Physics of Quantum Electronics, 6-10 January 2002

Special Issue: 32nd Winter Symposium on the Physics of Quantum Electronics, 6-10 January 2002

Call for Abstracts

Call for Abstracts

Announcement

ANNOUNCEMENTAnnouncementPublished Online:01 Oct 1998https://doi.org/10.1152/ajpcell.1998.275.4.C1178MoreSectionsPDF (36 KB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat The Miami Nature Biotechnology Winter Symposia The Miami Nature Biotechnology Winter Symposium on Signal Transduction and Therapeutic Strategies will be held February 6–10, 1999, at the Ramada Deauville Resort, Miami Beach, FL. The full program, registration forms, and poster and exhibitor information are available on the Web (http://bmb.med.miami. edu/mnbws.html). To receive a program by mail, please contact the Symposium at the following: 1 (305) 243 3597 (telephone), 1 (305) 324 5665 (Fax), or [email protected]med.miami.edu (E-mail).This article has no references to display. Download PDF Previous Back to Top Next FiguresReferencesRelatedInformationCited ByAssociation between skeletal muscle mass and cardiorespiratory fitness in community-dwelling elderly men18 June 2018 | Aging Clinical and Experimental Research, Vol. 31, No. 1Normal weight obesity and physical fitness in Chinese university students: an overlooked association4 December 2018 | BMC Public Health, Vol. 18, No. 1The aging influence on cardiorespiratory, metabolic, and energy expenditure adaptations in head-out aquatic exercises: Differences between young and elderly women16 March 2016 | Women & Health, Vol. 57, No. 3Maximal cardiac output in athletes: Influence of age16 January 2015 | European Journal of Preventive Cardiology, Vol. 22, No. 12Adverse effects of coexistence of sarcopenia and metabolic syndrome in Japanese women9 May 2012 | European Journal of Clinical Nutrition, Vol. 66, No. 10Cardiac response to exercise in young, normal weight and overweight men and women7 November 2008 | European Journal of Applied Physiology, Vol. 105, No. 3Muscle cross-sectional areas and performance power of limbs and trunk in the rowing motionSports Biomechanics, Vol. 6, No. 1Ageing and activity: their effects on the functional reserve capacities of the heart and vascular smooth and skeletal muscles20 February 2007 | Ergonomics, Vol. 48, No. 11-14Peak oxygen uptake during running and arm cranking normalized to total and regional skeletal muscle mass measured by magnetic resonance imaging20 November 2004 | European Journal of Applied Physiology, Vol. 93, No. 5-6Oxygen uptake and ventilation during rowing and running in females and malesScandinavian Journal of Medicine and Science in Sports, Vol. 13, No. 6Element Metabolism and Body Composition More from this issue > Volume 275Issue 4October 1998Pages C1178-C1178 Copyright & PermissionsCopyright © 1998 the American Physiological Societyhttps://doi.org/10.1152/ajpcell.1998.275.4.C1178PubMed9746464History Published online 1 October 1998 Published in print 1 October 1998 Metrics

Partially unfolded proteins efficiently penetrate cell membranes — implications for oral drug delivery

We have previously reported on the biological activity of members of a library of low molecular weight compounds (carriers) that enable the oral delivery of proteins (Milstein, Proceedings of the 1995 Miami Bio/Technology Winter Symposium on Protein Engineering and Structural Biology, IRL Press at Oxford University Press, 1995, p. 13; Leone-Bay et al., J. Med. Chem. 38 (1995) 4263–4269; Leone-Bay et al., J. Med. Chem. 39 (1996) 2571–2578; [1–3]). When rats or primates are orally administered a solution of carrier and either recombinant human α-interferon (rhIFN), insulin or recombinant human growth hormone (rhGH) significant serum concentrations of the proteins are detectable. The transport activity of these compounds is positively correlated with their structural effects on the protein molecules. Direct measurement of the interaction of these carrier molecules with the proteins indicates that they reversibly destabilize the native state of the molecule favoring a partially unfolded conformation. Apparently these intermediate protein conformations are transport competent and are able to be absorbed through the intestinal tissue and into the bloodstream. Since the measured binding of the carriers to the partially unfolded proteins is relatively weak ( K b=100 M −1) and the systemic activity of the proteins appears to be unaffected, the changes in the structure of the proteins are manifestly reversible.

Molecular medicine through the kaleidoscope

The 30th Miami Winter Symposium: Molecular Biology in the Conquest of DiseaseMiami, FL, USA, 7–11 February 1998

American Uveitis Society Winter Symposium, January 1997

American Uveitis Society Winter Symposium, January 1997

Agricultural Biotechnology '96

It has been 13 years since during the 15th Miami Winter Symposium the successful transformation of tobacco was announced. This date serves as a convenient milestone for the beginning of the age of agricultural biotechnology. The date is comparable to 1903 when the Wright brothers flew an airplane on the beaches of North Carolina or 1879 when Edison created the first successful light bulb. By 2003, when this technology has its 20th birthday, most of the corn, soybeans, cotton, tomatoes, potatoes and canola in North America will be genetically engineered. The traits will range from herbicide tolerance, insect and disease resistance to improvements in quality, and the improvements will serve to decrease farmer inputs, increase yields and improve the quality of our food and fiber. The new products will shift pesticide use toward cheaper, safer, more efficacious compounds and will result in less fuel use and decreased chemical volumes. And it will not stop there.. . The impact of the technology is accelerating at an astonishing pace. While the first-generation impact will likely be in crops, biologicals and novel pesticides, the next wave of products will include genetically modified microbes, along with novel, mode-of-action plant chemicals co-developed through traditional and biotechnology-based technologies. Improved germplasm is being developed at a faster and more efficient pace through marker-assisted breeding. Finally, a third wave of products are beginning to emerge as the technology is applied to enhancing the genetic base of farm animals. These were the messages delivered by university professors and industrial scientists at the IBC World Summit on Agricultural Biotechnology ‘96 held on March 4-5 in Santa Fe, New Mexico.

1993 Miami Bio/Technology Winter Symposium: Protein Engineering & Beyond Miami Beach, Florida January 17–22 Papers & Posters

1993 Miami Bio/Technology Winter Symposium: Protein Engineering & Beyond Miami Beach, Florida January 17–22 Papers & Posters

Tools for gene technology

A kit to avoid false positives with PCR and YAC vectors will feature at next week's Miami Bio/Technology Winter Symposium on the Molecular Biology of Human Genetic Disease to be held in Miami, Florida.