Winter Samples Research Articles

Benefit of the nutritional and mineral composition of sea lettuce from a traditional salina: Implications for human consumption

The proximal composition and its seasonal variation of the green seaweed Ulva sp. harvested in a traditional saline (earthen ponds used for marine salt extraction) from Cadiz Bay (Southern Spain) was evaluated. Ulva sp. was also collected in a reference location within the Bay in order to compare and evaluate the effects of the particular characteristics of the saline in the composition of the macroalgae. Moisture, protein, lipid, ash, carbohydrate, fiber and macro- (Na, K, Ca, Mg), micro-mineral contents (Fe, Zn, Cu) and heavy metals (As, Cd, Co, Cr, Hg, Ni, Pb, Sn) of harvested biomass samples as well as environmental parameters of seawater (temperature, salinity, pH, DO, NH4+, NO3−, NO2− and PO43−) were measured. The results showed that Ulva sp. from the earthen ponds in the traditional salina was a better source of proteins, lipids, K and Mg, highlighting in summer with values of 27.54 % versus 6.11 %; 6.71 % versus 3.26 %; 26.60 mg g−1 versus 14.21 mg g−1 and 23.13 mg g−1 versus 17.79 mg g−1, respectively. It also had Na/K and Ca/Mg ratios of less than one, suggesting a healthy food source. Considering the Commission Recommendation (EU) 2018/464 as a working reference, Ulva sp. did not exceed the limit of toxic metals for human consumption.A season and site-season significant interaction on the composition of the seaweeds was observed. The proximal and mineral composition of Ulva sp. was influenced by the special features and environmental conditions of the earthen ponds. Hence, significant differences were observed in the macroalgae collected in the earthen ponds in summer and autumn, in contrast to the winter and spring samples, whose characteristics were similar to those from the inner bay. The closure of the lock-gates in summer to favor the production of salt significantly modified the environmental characteristics of the saline, affecting the physiological capacity of Ulva sp. to assimilate and storage nutrients, and therefore its tissue composition. As a consequence, the highest contents of lipid, ash, Ca, K, Mg and Fe were estimated in the macroalgae.

Particulate matter annual emission factors for dairy facilities and cattle feedlots of Texas, USA

The direct emission of inhalable particulate matters (PM2.5 and PM10) from agricultural sources imposes major concern to human health. It is imperative to keep regular update of the pollutant emissions to scrutinize the sources and take necessary preventive measures. Two representative animal feeding operations, one dairy and one feedlot of Texas were selected during the year 2021 for summer and winter sampling using programmable Federal Reference Method (FRM) samplers and Texas A&M University (TAMU) designed samplers. Additionally, for quick and simple emission concentration determination, handheld particle counters were utilized parallel to the FRM and TAMU designed samplers. The air quality index showed that the PM2.5 pollutant fell into ‘Moderate’ category for about 20.93% times and 13.89% times in the dairy and the feedlot respectively. The maximum mean of daily PM10 concentration observed in the Feedlot during the summer was 1676.3 μg/m3 which can be aggravating. The correlation developed using the emission data from the FRM and handheld samplers demonstrated linear relationship and 0.66 of goodness of fit. During the summer, maximum mean PM2.5 emission factors (EF) of 0.84 kg 1000 hd−1 d−1 and 8.09 kg 1000 hd−1 d−1 were determined for the dairy and for the feedlot respectively. However, the winter and the summer PM10 EF for the dairy did not differ significantly (P > 0.05). Although there has not been enough record of PM2.5 annual EF, the current study PM10 annual EF was benchmarked with some of the previously published EFs. This investigation on the air pollutant EFs from the animal feeding operations can greatly benefit in preventing the pollution and updating the most recent EFs. However, continuous monitoring and further research are suggested to improve data quality and develop strategic management practices.

Simultaneous detection and characterization of common respiratory pathogens in wastewater through genomic sequencing

Genomic surveillance of SARS-CoV-2 has given insight into the evolution and epidemiology of the virus and its variant lineages during the COVID-19 pandemic. Expanding this approach to include a range of respiratory pathogens can better inform public health preparedness for potential outbreaks and epidemics. Here, we simultaneously sequenced 38 pathogens including influenza viruses, coronaviruses and bocaviruses, to examine the abundance and seasonality of respiratory pathogens in urban wastewater. We deployed a targeted bait capture method and short-read sequencing (Illumina Respiratory Virus Oligos Panel; RVOP) on composite wastewater samples from 8 wastewater treatment plants (WWTPs) and one associated hospital site. By combining seasonal sampling with whole genome sequencing, we were able to concurrently detect and characterise a range of common respiratory pathogens, including SARS-CoV-2, adenovirus and parainfluenza virus. We demonstrated that 38 respiratory pathogens can be detected at low abundances year-round, that hospital pathogen diversity is higher in winter vs. summer sampling events, and that significantly more viruses are detected in raw influent compared to treated effluent samples. Finally, we compared detection sensitivity of RT-qPCR vs. next generation sequencing for SARS-CoV-2, enteroviruses, influenza A/B, and respiratory syncytial viruses. We conclude that both should be used in combination; RT-qPCR allowed accurate quantification, whilst genomic sequencing detected pathogens at lower abundance. We demonstrate the valuable role of wastewater genomic surveillance and its contribution to the field of wastewater-based epidemiology, gaining rapid understanding of the seasonal presence and persistence for common respiratory pathogens. By simultaneously monitoring seasonal trends and early warning signs of many viruses circulating in communities, public health agencies can implement targeted prevention and rapid response plans.

Detection and High-Throughput Microbial Analysis of Particulate Matter in Houses and Downwind Areas of Duck Farms

Particulate matter (PM) and the microorganisms of duck houses may have negative impacts on animal and human health. During 2021-2022, PM2.5 and PM10 inside and outside the duck house were sampled with a built-in air sampler in Tai’an City, Shandong Province, and the diversity and abundance of microorganisms within the PM were analyzed by macrogenomic and absolute sequence analysis. The results showed that PM2.5 and PM10 concentrations in the house and at downwind points exceeded the short-term (24 h) guideline of the global air quality guidelines (AQG). Macrogenome sequencing showed that the microbial composition of the PM2.5 samples was dominated by bacteria (exceed 85%); a total of 1316 bacterial genera and 110 fungal genera were identified in PM2.5 samples from duck house 1 in winter, which were much higher than the results of amplicon sequencing method reported before, and relatively high levels of the pathogenic bacteria (Coccidioides immitis, etc.) and the conditionally pathogenic bacterium (Rothia nasimurium) were identified at the species level. Absolute quantitative sequencing detected conditionally pathogenic bacteria and allergens at high levels in PM10 samples: Corynebacterium (5.6×107 copies/g), Aerococcus (9.9×106 copies/g), Alternaria (3.3×106 copies/g), and Aspergillus (8.3×105 copies/g). Moreover, Corynebacterium was the highest content of PM10 in summer and PM2.5 samples in winter, and its pathogenicity and potential threat should be noted. The diversity and relative abundance of microorganisms were similar in the duck house and at the downwind point. The results showed that the microorganisms in the house environment have a greater influence on the air environment around the downwind point and may pose a public health risk to the staff and the surrounding area.

Strategic control of combustion-induced ammonia emissions: A key initiative for substantial PM2.5 reduction in Tianjin, North China Plain

Information on the temporal and spatial variations in the sources of ammonium salts (NH4+), a crucial alkaline component in PM2.5, is limited. Here, we simultaneously collected PM2.5 and gaseous ammonia (NH3) samples in both summer and winter from two sites in Tianjin: an urban site (Tianjin University, TJU) and a suburban site (Binhai New-region, BH). NH3 concentrations, the contents of major water-soluble inorganic ions in PM2.5, and the compositions of ammonium‑nitrogen isotopes (δ15N-NH4+) were measured. As a result, (NH4)2SO4 and NH4NO3 were the predominant forms of NH4+ in PM2.5 during summer and winter, respectively. However, the NH4NO3 concentrations were notably greater at TJU (6.2 ± 7.3 μg m−3) than at BH (3.8 ± 4.7 μg m−3) in summer, with no regional differences observed in winter. Both sites displayed almost half the contribution of c-NH3 (combustion-related NH3) to NH4+, differing from the finding of previous isotope-based studies. This discrepancy could be attributed to the combined effects of NHx isotope fractionation and seasonal δ15N value variations in NH3 sources. The contribution fractions of v-NH3 (volatile NH3) and c-NH3 exhibited similar patterns at both sites seasonally, probably caused by coal combustion for heating in winter and temperature fluctuations. However, the contribution fraction of c-NH3 was lower at BH than at TJU in summer but greater in winter than at TJU. In summer, NH4NO3 was unstable and limited its delivery to TJU from BH, and the high contribution of c-NH3 to NH4+ at TJU could be attributed to local vehicle emissions. In winter, the stable particulate NH4NO3 that formed from the c-NH3 in the upwind area could be transported to the downwind area, increasing the NH4+ concentration at BH. Our study provides valuable insights for devising emission mitigation strategies to alleviate the increasing burden of NH3 in the local atmosphere.

Evaluation of the health of Mediterranean mussels (Mytilus galloprovincialis Lamarck, 1819) distributed in the Çanakkale strait, Turkey

The observation of mortality in Mediterranean mussels (Mytilus galloprovincialis) distributed in the Çanakkale Strait in recent years was influential in developing the research question for this study. In this study, the presence of bacteria (Vibrio spp.) and parasites (Marteilia spp. and Haplosporidium spp.) in mussels collected from Kumkale, Kepez, and Umurbey stations in the Çanakkale Strait was investigated seasonally. Microbiological findings, histopathology, oxidative stress enzymes and their gene expressions, lipid peroxidation, lysosomal membrane stability, and changes in haemolymph were examined. In summer samples, both the defence system and the extent of damage were higher in gill tissue. In winter samples, enzyme activities and lipid peroxidation were found to be predominantly higher in digestive gland tissues. Histological examinations and Hemacolor staining revealed the presence of protozoan cysts, and for bacterial examination, molecular analysis performed after culturing revealed the presence of 7 Vibrio species. While the total numbers of heterotrophic bacteria detected in all samples were at acceptable levels, the predominance of Vibrio spp. numbers among the total heterotrophic bacteria detected in almost all samples were noteworthy. The total hemocyte count was calculated as 5.810(4)±0.58 (cells/mm3) in winter and 7.210(4)±1.03 (cells/mm3) in summer. These factors are considered to be possible causes of mussel mortality.

Redox-activity and in vitro effects of regional atmospheric aerosol pollution: Seasonal differences and correlation between oxidative potential and in vitro toxicity of PM1

Particulate Matter (PM) is a complex and heterogeneous mixture of atmospheric particles recognized as a threat to human health. Oxidative Potential (OP) measurement is a promising and integrative method for estimating PM-induced health impacts since it is recognized as more closely associated with adverse health effects than ordinarily used PM mass concentrations. OP measurements could be introduced in the air quality monitoring, along with the parameters currently evaluated. PM deposition in the lungs induces oxidative stress, inflammation, and DNA damage.The study aimed to compare the OP measurements with toxicological effects on BEAS-2B and THP-1 cells of winter and summer PM1 collected in the Po Valley (Italy) during 2021. PM1 was extracted in deionized water by mechanical agitation and tested for OP and, in parallel, used to treat cells. Cytotoxicity, genotoxicity, oxidative stress, and inflammatory responses were assessed by MTT test, DCFH-DA assay, micronucleus, γ-H2AX, comet assay modified with endonucleases, ELISA, and Real-Time PCR. The evaluation of OP was performed by applying three different assays: dithiothreitol (OPDTT), ascorbic acid (OPAA), and 2′,7′-dichlorofluorescein (OPDCFH), in addition, the reducing potential was also analysed (RPDPPH).Seasonal differences were detected in all the parameters investigated. The amount of DNA damage detected with the Comet assay and ROS formation highlights the presence of oxidative damage both in winter and in summer samples, while DNA damage (micronucleus) and genes regulation were mainly detected in winter samples. A positive correlation with OPDCFH (Spearman's analysis, p < 0.05) was detected for IL-8 secretion and γ-H2AX.These results provide a biological support to the implementation in air quality monitoring of OP measurements as a useful proxy to estimate PM-induced cellular toxicological responses. In addition, these results provide new insights for the assessment of the ability of secondary aerosol in the background atmosphere to induce oxidative stress and health effects.

Identification of the estrogen-active compounds via integrating effect-directed analysis and non-target screening in soils of the northeastern China

BackgroundThe gaps between estrogenic effect and its effect-active compounds exist frequently due to a large number of compounds that have been reported to induce this effect and the occurrence of pollutants in environments as mixtures. Therefore, identifying the estrogen-active compounds is of importance for environmental management and pollution treatment. In the current study, the effect-directed analysis (EDA) and non-targeted screening (NTS) were integrated to identify the estrogen-active compounds in soils of the rural area with different socioeconomic types (industrial, farming and plantation village) in Northeast China.ResultsThe cytotoxicity results indicated that the industrial and farming villages showed cytotoxic effects. The detection rates of estrogenic effects for samples of winter and summer were 100% and 87%, respectively. Of which, the effects were found to be stronger in summer than in winter, with significant difference observed from the farming village (0.1–11.3 EEQ μg/kg dry weight). A total of 159 chemicals were detected by NTS. By integrating EDA, triphenyl phosphate (TPhP) and indole were successfully identified from a raw sample and its fraction, explaining up to 19.31% of the estrogen activity.ConclusionsThe present study demonstrates that the successful identification of seven estrogen-active compounds in rural areas of northeastern China can be achieved through the combination of effect-directed analysis (EDA) and non-targeted screening (NTS). This finding is beneficial for risk monitoring and pollution management.

Variability in molecular composition and optical absorption of atmospheric brown carbon aerosols in two contrasting urban areas of China

Atmospheric brown carbon (BrC) aerosols were investigated at two urban sites in southern (Hefei) and northern (Shijiazhuang) China during summer and winter of 2019–2020 to explore regional variability in their compositional and optical properties. Organic matter in ambient PM2.5 samples were characterized at molecular level using ultrahigh performance liquid chromatography coupled with a diode array detector and an Orbitrap mass spectrometer. Although the molecular composition of organic aerosols varied substantially over different ambient environments, they were mainly composed by CHO and CHON species in positive ionization mode while CHO and CHOS species in negative mode. The mass absorption coefficients of BrC aerosols at wavelength range 250–450 nm were relatively higher for winter samples in both cities and for Shijiazhuang samples in both seasons, partly attributed to the higher concentration levels of anthropogenic air pollutants in these environments. The absorption Ångström exponents further revealed that BrC aerosols in winter seasons and in Shijiazhuang had a greater capacity of absorption at shorter wavelengths. A total of 26 BrC species with strong absorption were unambiguously identified from different environments, which mainly consisted of CHO, CHON, and CHN species and had higher degrees of unsaturation and lower degrees of oxidation. The presence and abundance of these BrC species varied dynamically across the seasons and cities, with a greater number of species presented in the winter of Shijiazhuang. The BrC species together contributed 12–26 % in the total absorbance of light-absorbing organic components at 250–450 nm. This study highlights the regional differences in BrC properties influenced by the sources and atmospheric processes, which should be taken into account to assess their climate impacts.

Integrated biomarker responses in wild populations of the intertidal sea anemone Bunodosoma zamponii living under different anthropogenic pressures.

Bunodosoma zamponii is the most abundant anemone in Mar del Plata (Buenos Aires, Argentina). Given that the presence of persistent organic pollutants (organochlorine pesticides and PCBs) and the organophosphate pesticide chlorpyrifos has recently been reported in this species, two wild populations living under different anthropogenic pressures were studied and compared regarding basic aspects of their ecology and physiological response to oxidative stress. A population from an impacted site (Las Delicias, LD) and another from a reference site (Punta Cantera, PC) were monitored seasonally (spring, summer, autumn, and winter), for one year. Anemones from PC were larger and more abundant than those from LD for most sampling periods. During winter, glutathione-S-transferase and catalase activities were higher in LD. Moreover, protein content and antioxidant defenses were higher in anemones from PC during winter as well. Taking into account their ecology (size and abundance) and biomarker responses, the population from PC was comparatively healthier. Furthermore, such differences are in agreement with recent studies indicating a higher concentration of pollutants in anemones from LD (specially during the winter sampling). In this sense, considering that B. zamponii can bioaccumulate the aforementioned pollutants, its resilience to their presence, and the fact that biomarker response differed between sites, this species can be regarded as a proper sentinel species of environmental pollution. Overall, this anemone seems to be a good bioindicator to be considered in future biomonitoring and ecotoxicological studies.

Estimation of spawning stock biomass and spawning areas of sardine, (Sardina pilchardus) with winter time ichthyoplankton sampling in the Sea of Marmara, Türkiye

The spawning areas and spawning stock biomass of sardine were studied with ichthyoplankton sampling in the winter period from the 32 stations, in the Marmara Sea. The mean fish egg and larvae biomass in a unit area were calculated as 18.4 ± 5.3 eggs/10 m2 and 2.5 larvae/10 m2, respectively. Three main spawning areas were detected as Karacabey Floodplain area, Gönen, and Büyükçekmece estuarine area. The larvae are mostly located in the western part. The batch fecundity of sardine was detected between 2415.9 and 16738.3, with a mean of 6899.8 ± 255.7 eggs. The sex ratio (R), spawning fraction (S), mortality rate, and daily egg production (Po) were calculated as 0.53, 0.098, 0.62, and 9.25 eggs/m2 in the Marmara Sea. The spawning stock biomass (B) is estimated at 2998 tonnes in the Marmara Sea. Both ichthyoplankton biomass and spawning stock biomass were found relatively lower. It is recommended to apply stricter management sanctions for the sustainability of sardine stocks.

Greenhouse gas production from an intermittently dosed cold-climate wastewater treatment wetland

This study explores the greenhouse gas (GHG) fluxes of nitrous oxide (N2O), methane (CH4) and carbon dioxide (CO2) from a two-stage, cold-climate vertical-flow treatment wetland (TW) treating ski area wastewater at 3 °C average water temperature. The system is designed like a modified Ludzack-Ettinger process with the first stage a partially saturated, denitrifying TW followed by an unsaturated nitrifying TW and recycle of nitrified effluent. An intermittent wastewater dosing scheme was established for both stages, with alternating carbon-rich wastewater and nitrate-rich recycle to the first stage. The system has demonstrated effective chemical oxygen demand (COD) and total inorganic nitrogen (TIN) removal in high-strength wastewater over seven years of winter operation. Following two closed-loop, intensive GHG winter sampling campaigns at the TW, the magnitude of N2O flux was 2.2 times higher for denitrification than nitrification. CH4 and N2O emissions were strongly correlated with hydraulic loading, whereas CO2 was correlated with surface temperature. GHG fluxes from each stage were related to both microbial activity and off-gassing of dissolved species during wastewater dosing, thus the time of sampling relative to dosing strongly influenced observed fluxes. These results suggest that estimates of GHG fluxes from TWs may be biased if mass transfer and mechanisms of wastewater application are not considered. Emission factors for N2O and CH4 were 0.27 % as kg-N2O-N/kg-TINremoved and 0.04 % kg-CH4-C/kg-CODremoved, respectively. The system had observed seasonal emissions of 600.5 kg CO2 equivalent of GHGs estimated over 130-days of operation. These results indicate a need for wastewater treatment processes to mitigate GHGs.

Occurrence and fate of pharmaceuticals and personal care products in a wastewater treatment plant with Bacillus bio-reactor treatment

Pharmaceuticals and personal care products (PPCPs) have attracted wide attention due to their environmental impacts and health risks. PPCPs released through wastewater treatment plants (WWTPs) are estimated to be 80 %. Nevertheless, the occurrence of PPCPs in the WWTPs equipped with Bacillus spec.-based bioreactors (BBR) treatment system remains unclear. In this study, sludge and waste water samples were collected during separate winter and summer sampling campaigns from a typical BBR treatment system. The results indicate that out of 58 target PPCPs, 27 compounds were detected in the waste water (0.06–1900 ng/L), and 23 were found in the sludge (0.6–7755 ng/g dw). Paraxanthine was the chemical of the highest abundance in the influent due to the high consumption of the parent compounds caffeine and theobromine. The profile for PPCPs in the wastewater and sludge exhibited no seasonal variation. Overall, the removal of target PPCPs in summer is more effective than the winter. In the BBR bio-reactor, it was found that selected PPCPs (at ng/L level) can be completely removed. The efficiency for individual PPCP removal was increased from 1.0 % to 50 % in this unit, after target specific adjustments of the process. The effective removal of selected PPCPs by the BBR treatment system is explained by combined sorption and biodegradation processing. The re-occurrence of PPCPs in the wastewater was monitored. Negative removal efficiency was explained by the cleavage of Phase II metabolites after the biotransformation process, and the lack of equilibrium for PPCPs in the sludge of the second clarifier. A compound specific risk quotient (RQ) was calculated and applied for studying the potential environmental risks. Diphenhydramine is found with the highest environmental risk in wastewater, and 15 other PPCPs show negligible risks in sewage sludge.

Nutrient structure dynamics and microbial communities at the water-sediment interface in an extremely acidic lake in northern Patagonia.

Lake Caviahue (37° 50 'S and 71° 06' W; Patagonia, Argentina) is an extreme case of a glacial, naturally acidic, aquatic environment (pH ~ 3). Knowledge of the bacterial communities in the water column of this lake, is incipient, with a basal quantification of the bacterioplankton abundance distribution in the North and South Basins of Lake Caviahue, and the described the presence of sulfur and iron oxidizing bacteria in the lake sediments. The role that bacterioplankton plays in nutrient utilization and recycling in this environment, especially in the phosphorus cycle, has not been studied. In this work, we explore this aspect in further depth by assessing the diversity of pelagic, littoral and sediment bacteria, using state of the art molecular methods and identifying the differences and commonalties in the composition of the cognate communities. Also, we investigate the interactions between the sediments of Lake Caviahue and the microbial communities present in both sediments, pore water and the water column, to comprehend the ecological relationships driving nutrient structure and fluxes, with a special focus on carbon, nitrogen, and phosphorus. Two major environmental patterns were observed: (a) one distinguishing the surface water samples due to temperature, Fe2+, and electrical conductivity, and (b) another distinguishing winter and summer samples due to the high pH and increasing concentrations of N-NH4+, DOC and SO42-, from autumn and spring samples with high soluble reactive phosphorus (SRP) and iron concentrations. The largest bacterial abundance was found in autumn, alongside higher levels of dissolved phosphorus, iron forms, and increased conductivity. The highest values of bacterial biomass were found in the bottom strata of the lake, which is also where the greatest diversity in microbial communities was found. The experiments using continuous flow column microcosms showed that microbial growth over time, in both the test and control columns, was accompanied by a decrease in the concentration of dissolved nutrients (SRP and N-NH4+), providing proof that sediment microorganisms are active and contribute significantly to nutrient utilization/mobilization.

Atmospheric CO2 and 14CO2 observations at the northern foot of the Qinling Mountains in China: Temporal characteristics and source quantification

A two-year (March 2021 to February 2023) continuous atmospheric CO2 and a one-year regular atmospheric 14CO2 measurement records were measured at the northern foot of the Qinling Mountains in Xi'an, China, aiming to study the temporal characteristics of atmospheric CO2 and the contributions from the sources of fossil fuel CO2 (CO2ff) and biological CO2 (CO2bio) fluxes. The two-year mean CO2 mole fraction was 442.2 ± 16.3 ppm, with a yearly increase of 4.7 ppm (i.e., 1.1 %) during the two-year observations. Seasonal CO2 mole fractions were the highest in winter (452.1 ± 17.7 ppm) and the lowest in summer (433.5 ± 13.3 ppm), with the monthly CO2 levels peaking in January and troughing in June. Diurnal CO2 levels peaked at dawn (05:00–07:00) in spring, summer and autumn, and at 10:00 in winter. 14C analysis revealed that the excess CO2 (CO2ex, atmospheric CO2 minus background CO2) at this site was mainly from CO2ff emissions (67.0 ± 26.8 %), and CO2ff mole fractions were the highest in winter (20.6 ± 17.7 ppm). Local CO enhancement above the background mole fraction (ΔCO) was significantly (r = 0.74, p < 0.05) positively correlated with CO2ff in a one-year measurement, and ΔCO:CO2ff showed a ratio of 23 ± 6 ppb/ppm during summer and winter sampling days, much lower than previous measurements and suggesting an improvement in combustion efficiency over the last decade. CO2bio mole fractions also peaked in winter (14.2 ± 9.6 ppm), apparently due to biomass combustion and the lower and more stable wintertime atmospheric boundary layer. The negative CO2bio values in summer indicated that terrestrial vegetation of the Qinling Mountains had the potential to uptake atmospheric CO2 during the corresponding sampling days. This site is most sensitive to local emissions from Xi'an and to short distance transportation from the southern Qinling Mountains through the valleys.

Biofilms and Biodeterioration: The Case of an Ancient Indian Monument

Historical buildings are substantially built with sedimentary rocks, especially limestone, and form an important component of a country’s cultural heritage. The monuments are exposed to external environmental forces (humidity and temperature) that facilitate the proliferation of microorganisms and biofilms in an ecological succession. Feroz Shah Kotla is an ancient monument in Delhi, India, dating back to 1354 ad, and constructed of limestone, and with a discoloured façade now. The main objective of the present study was to characterise the biofilm present in areas of monuments exposed to sun and shaded areas, as influenced by two seasons—winter and monsoon (rainy), in terms of moisture, chlorophyll, protein and exopolysaccharide (EPS) as a function of carbohydrate concentration. The moisture, protein and EPS contents were higher in samples obtained from sun-exposed areas of rainy season samples and least in shaded areas of winter samples, while the chlorophyll content was highest in sun-exposed winter samples. The ICP-MS analysis showed that Ca concentration was highest (15,967.92 µg−1). Scanned electron microscope images show the presence of coccoid, bacillus and filamentous forms of microorganisms, and sparser in the dry season. The results of the present study provide significant clues that the deterioration of such ancient monuments is correlated to the establishment and continued presence of biofilms which eventually weaken and corrode the surface; appropriate conservation actions can be congruent in targeting biofilms successfully.

Temporal patterns of bacterial communities in the Billings Reservoir system

In this study, high-throughput sequencing of 16S rRNA amplicons and predictive PICRUSt functional profiles were used to perform a comprehensive analysis of the temporal bacterial distribution and metabolic functions of 19 bimonthly samples collected from July 2019 to January 2020 in the surface water of Billings Reservoir, São Paulo. The results revealed that most of the bacterial 16S rRNA gene sequences belonged to Cyanobacteria and Proteobacteria, which accounted for more than 58% of the total bacterial abundance. Species richness and evenness indices were highest in surface water from summer samples (January 2020), followed by winter (July 2019) and spring samples (September and November 2019). Results also showed that the highest concentrations of sulfate (SO4–2), phosphate (P), ammonia (NH3), and nitrate (NO3-) were detected in November 2019 and January 2020 compared with samples collected in July and September 2019 (P < 0.05). Principal component analysis suggests that physicochemical factors such as pH, DO, temperature, and NH3 are the most important environmental factors influencing spatial and temporal variations in the community structure of bacterioplankton. At the genus level, 18.3% and 9.9% of OTUs in the July and September 2019 samples, respectively, were assigned to Planktothrix, while 14.4% and 20% of OTUs in the November 2019 and January 2020 samples, respectively, were assigned to Microcystis. In addition, PICRUSt metabolic analysis revealed increasing enrichment of genes in surface water associated with multiple metabolic processes rather than a single regulatory mechanism. This is the first study to examine the temporal dynamics of bacterioplankton and its function in Billings Reservoir during the winter, spring, and summer seasons. The study provides comprehensive reference information on the effects of an artificial habitat on the bacterioplankton community that can be used to interpret the results of studies to evaluate and set appropriate treatment targets.

Deciphering variations in the surficial bacterial compositions and functional profiles in the intersection between North and South Yellow Sea

The coastal ocean systems play paramount role in the nutrient biogeochemistry because of its interconnected environment. To gain a novel insight into coupling relationships between bacterial community, functioning properties and nutrient metabolism, we conducted analysis on the patterns and driving factors of planktonic bacterial functional community across subsurface water of marine ranching near the Yellow Sea in both summer and winter. Illumina HiSeq Sequencing and a corresponding set of biogeochemical data were used to assess distribution patterns of taxa, adaptive mechanism and metabolic function. Results demonstrated that Proteobacteria, Cyanobacteria, Actinobacteriota and Bacteroidota were dominant phyla both in summer and winter. Taxonomic profiles related to nutrient variation were found to be highly correlated with Dissolved Oxygen (DO) and Chlorophyll fluorescence (FLUO), and distinct diversity differences were also found between summer and winter samples. Functional activity in summer associated with the relative abundance of phototrophy and photoautotrophy were the highest in the subsurface water, while in winter the dominant functional properties were mainly include chemoheterotrophy and aerobic_ chemoheterotrophy. A significant difference related to functional activity between summer and winter, mainly representing ligninolysis and iron_respiration. In general, our study provides a framework for understanding the relative importance of environmental factors, temperature variation and nutrient availability in shaping the metabolic processes of aquatic microorganisms, particularly in ocean mariculture systems.

Seasonal and taxa impact on edible sea cucumber's metabolome and in relation to its cytotoxicity as analyzed via UHPLC/HR‐MS/MS‐based molecular networking and chemometrics

AbstractSea cucumbers, belonging to class Holothuroidea, are marine invertebrates with substantial bioactive compounds. This study provides the first comparative metabolome profiling of Holothuria atra and Actinopyga crassa sea cucumbers in relation to their season of collection to dissect between winter and summer samples, providing several first‐time reported metabolites as well as the effect of metabolome variation on their cytotoxic activity against colon and prostate cancer cell lines. Ultrahigh‐performance liquid chromatography coupled with high‐resolution mass spectrometry and global natural products social molecular networking followed by multivariate data analysis were employed for metabolite profiling and seasonal biomarker assignment for each species. The cytotoxic activity was evaluated using in vitro assays against prostate and colon cancer cell lines. A total of 113 metabolites were annotated in both species, including 4 organic acids, 21 sulfoquinovosyl glycerol (sulfolipids), 5 alkyl sulfates, 13 sulfated/saponins, 8 sulfated/triterpene mono/di‐glycosides, 10 triterpenes, 13 sulfated/sterols, 32 phospholipids, 4 sphingolipids, and 3 glycerolipids, several of which are new or first‐time reported. Multivariate data analyses revealed lipid accumulation in summer samples versus triterpene and saponin enrichment in winter‐collected samples, particularly in H. atra, rationalizing its potent cytotoxic activity (IC50 6–12 µg/mL) as revealed using a regression model. However, winter‐collected samples of A. crassa are relatively the least abundant in antinutrient saponins, thus considered to have higher food value.

Evaluating real-world emissions from in-use buses and taxis using on-road remote sensing

Assessing real-world emissions from buses and taxis is vital to comprehend their impact on urban air quality. Such vehicles differ significantly from the majority of the fleet owing to their higher mileage rates. However, few studies have focused on specifically assessing the emissions from this segment of the vehicle fleet. In this context, this study evaluated the real-world emissions of nitrogen oxides (NOx) from in-use buses and taxis in Dublin, Ireland, using crossroad remote sensing technology. The remote sensing system was deployed at strategic locations throughout the city to capture on-road emissions from passing vehicles. The collected data included vehicle related information such as emission standard, make, and mileage, and pollutants including NOx. Based on this data, analysis was aimed to understand the impact of Euro emission standard, ambient temperature, mileage, and make of the vehicle on NOx emissions. The results reveal that the average emissions from taxis reduce by 37% from Euro 5 to Euro 6b, and average emissions from Euro 6 buses are 87% lower compared to Euro 5. The trends in emission factors (EFs) of buses and taxis were similar during summer and winter sampling. Moreover, on comparing the emissions from the top five taxi manufacturers, different trends in the emission factors were observed. Finally, the study found that the effect of vehicle mileage on emissions was unclear for both buses and taxis. In any case, these findings provide valuable insights into the real-world emission performance of the existing fleet of buses and taxis in Dublin and highlight the need for targeted measures to reduce emissions from these vehicles. The results can assist policymakers and urban planners in formulating evidence-based strategies to improve air quality in Dublin and other cities facing similar challenges.