Winter-early Spring Research Articles

Within‐sex density dependence and population dynamics of red squirrels Sciurus vulgaris

Summary Social organization and dispersal of red squirrels (Sciurus vulgaris L.) differ between sexes, and intrasexual competition is intense. Therefore, we predicted that demographic parameters should be gender‐specific: that is density‐dependent factors will be more strongly related to density of the same sex than to density of the opposite sex. We studied the relative importance of within‐ and between‐sex density‐dependent factors and of density‐independent factors (habitat type, food abundance, winter temperature) on different demographic parameters, in two populations in northern Belgium. Spring density of males was positively correlated with tree‐seed abundance in the previous year, but this was not the case for females. None of the population parameters we measured differed between habitats, indicating that the same density‐dependent and density‐independent mechanisms prevailed in coniferous and deciduous habitat. Within each sex, we found several demographic parameters that were dependent on the densities of the same sex; however, none of these parameters was found to be dependent on the density of the opposite sex. Reproductive rate increased with food abundance and decreased with female density. Adult survival of females decreased with female density in autumn–winter, while survival of adult males in spring–summer increased with the size of the previous year's seed crop. Immigration rate of males was higher in spring than in autumn, and spring immigration increased with food abundance. Male recruitment rate, in both seasons, increased with food abundance, but was male density dependent. However, spring–summer loss rates also increased when food supplies were good, suggesting that despite high food availability, emigration of juvenile and subadult males increased when intrasexual competition was intense. Recruitment rate of females decreased with increasing female density. After a good seed crop, more subadult females dispersed, but their settlement success (recruitment) was lower at high female density. Seed crop size positively affected red squirrel densities through increased reproduction, immigration and adult survival of males, but density‐dependent reproduction and within‐sex density‐dependent recruitment of locally born juveniles and dispersing subadults limit the fluctuations in numbers and regulate densities in winter–early spring, as well as in summer.

Effects of temperature and application rate of a nitrification inhibitor, dicyandiamide (DCD), on nitrification rate and microbial biomass in a grazed pasture soil

Abstract. The nitrification inhibitor dicyandiamide (DCD) has recently been shown to be effective in reducing nitrate leaching from grazed pasture soils. The objective of this study was to determine the influence of temperature and application rate on the effectiveness of DCD in nitrification inhibition. Possible effects on soil microbial biomass were also determined. The soil, Lismore silt loam (Pallic orthic brown soil; Udic Haplustept loamy skeletal), was incubated at a moisture content near field capacity under 2 temperatures (8 or 20°C). Urea was applied at 25 kg N/ha and dairy cow urine at 1000 kg N/ha. DCD was applied at 2 rates equivalent to 7.5 or 15 kg/ha. The results show that at a soil temperature of 8°C, the half-life of DCD was 111–116 days. The half-life of NH4+ changed from 44 days without DCD to 243–491 days when DCD was applied. In contrast, at a soil temperature of 20°C the half-life of DCD was 18–25 days. The half-life of NH4+ changed from 22 days without DCD to 64–55 days with DCD. The 2 different rates of DCD had a small effect on the NH4+ concentration in the soil. The application of DCD did not have a significant effect on soil microbial biomass. DCD would therefore be most effective in inhibiting nitrification and thus reducing nitrate leaching in late autumn–winter–early spring in most parts of New Zealand when daily average soil temperatures are generally below 10°C and when drainage is high.

Seasonal variations in the physiological condition of the benthic amphipods Monoporeia affinis and Pontoporeia femorata in the Gulf of Riga (Baltic Sea)

Seasonal variations in the physiological condition of two benthic deposit-feeding amphipods were studied in two environmentally differing areas in the Gulf of Riga (Baltic Sea) with the purpose of estimating their role in benthic mineralisation. At the shallower (26 m) nearshore station, Monoporeia affinis exhibited low ammonia excretion rate (VNH 4 + ) throughout the year. After intensive accumulation of lipids in spring, the lipid reserves of the individuals became rapidly depleted during autumn. Gravid females in winter-early spring showed exceedingly low lipid and triacylglycerol (TAG) levels compared to studies carried out in open-sea areas. Pontoporeia femorata studied at the deeper (43 m) offshore station exhibited a higher VNH 4 + at all times with no significant seasonal variation. Lipid and TAG accumulated continuously until November, reaching higher levels than recorded previously for this species. Experiments showed that the temperature coefficient Q10 determined for VNH 4 + is likely to depend on the recent feeding history of the amphipods. Mineralisation of nitrogen and carbon by the amphipod populations at the study sites was estimated to be 1.7-3.0 g C and 11.3-13.5 mmol NH 4 + m–2 yr–1. Conclusively, both biotic and abiotic factors affect the condition of the amphipods, while the species also have genuine differences in their metabolic characteristics.

Recent, Large-Scale Changes in the Extratropical Southern Hemisphere Atmospheric Circulation

The zonal anomalies in the 500-hPa geopotential height field of the Southern Hemisphere are examined using the National Centers for Environmental Prediction–National Center for Atmospheric Research reanalyses, 1958–96. The zonal anomalies were constructed by removing the zonal mean from each grid point, and principal components analysis was performed on the resulting spatial anomalies. The principal components of the leading EOF of the seasons March–April–May (MAM) to September–October–November (SON) indicate that a change has occurred in the extratropical, large-scale, atmospheric circulation of the Southern Hemisphere. It is estimated to have begun after 1975 and is limited to the southern late fall, winter, and early spring. The elements of this change are a deeper Pacific trough and higher Indian Ocean ridge at subtropical latitudes. At higher latitudes the ridge over the Pacific strengthened and shifted east while troughing occurred in the southwest Atlantic–Weddell Sea region. Consistent with the changes in the zonal anomalies, zonal wave 1 experienced a large increase in amplitude in the late winter–early spring. On the other hand, zonal wave 3 reached its maximum one month earlier, shrank more quickly, and reached lower-than-average amplitudes in spring after 1975.

Correlation between the presence of Gonyaulax fragilis (Dinophyceae) and the mucilage phenomena of the Emilia-Romagna coast (northern Adriatic Sea)

The formation of massive amounts of suspended mucilaginous organic matter which periodically affects the Adriatic Sea, has been regarded as a complex physico-chemical phenomenon resulting from the production of extracellular material by phytoplankton. Although the exact cause has remained obscure, the mechanism of its formation has usually been considered to be a long-term process, starting after the late winter-early spring blooms, and involving the participation of various algal species, mainly within the diatom group. In this paper we report on the results of a phytoplankton monitoring programme in northern Adriatic seawaters off the Emilia-Romagna coast of Italy which revealed the constant concomitant presence of the dinoflagellate Gonyaulax fragilis (Schütt) Kofoid and mucilaginous formations. In the early stages of the phenomenon the dinoflagellate was clearly observable by microscopic examination in the mucilage, but as the mucilage aged this alga almost completely decomposed and diatom cells increased in number and became predominant. Although characterized by a slow growth rate in culture, in natural seawater G. fragilis was observed to reach cell densities of up to 7.0×10 6 cells l −1. The results of this study lead us to propose the hypothesis that the appearance of mucilage in the water column of the Adriatic Sea is the consequence of a seasonal growth of this dinoflagellate favoured by specific environmental circumstances.

Development and recovery from winter embolism in silver birch: seasonal patterns and relationships with the phenological cycle in oceanic Scotland.

Silver birch (Betula pendula Roth) is increasingly used in the United Kingdom for reforestation. However, recent evidence indicates that, under some circumstances, planted birch can suffer serious and repeated mortality of the apical leaders and branches, with consequent loss of apical dominance and the formation of a contorted stem. Plants from 37 seed sources of silver birch from Scotland and northern England planted at two sites were compared for several characteristics related to hydraulic architecture, vulnerability to freeze-thaw cycle induced embolism and spring recovery from winter embolism during the period 2000-2002. Phenological rhythms were also monitored in late winter-early spring to document relationships between phenology and water relations parameters. Significant differences were found across seed sources in stage of bud flushing for four dates in spring. Early flushing seed sources differed by about 1 to 2 weeks from late-flushing seed sources across the two sites. Wintertime xylem embolism in stems reached a peak of about 50 to 70% loss of xylem hydraulic conductivity, depending on the size and position of the sample shoots in the canopy. Small apical shoots were significantly more embolized than large basal shoots. Development of winter embolism was coupled to the occurrence of frost events. As percent loss of hydraulic conductivity increased during the winter, wood relative water content declined. Embolism reversal occurred rapidly in spring at the time of development of positive root pressure. No significant differences in the degree of winter embolism in 2001 were found among the three seed sources examined. The investigation was expanded in the winter-spring of 2002 to include 10 seed sources across both sites. Significant differences were found in degree of winter embolism across sites, dates and seed sources. For each date, there was a significant relationship between flushing scores and wood relative water contents across the two sites and all seed sources, suggesting that differences in time of flushing across sites and seed sources were likely caused by differences in the time of occurrence of root pressure, a necessary precondition to flushing.

Seasonal and spatial variations in fish and macrocrustacean assemblage structure in Mad Island Marsh estuary, Texas

Fish and macrocrustacean assemblage structure was analyzed along an estuarine gradient at Mad Island Marsh (MIM), Matagorda Bay, TX, during March 1998–August 1999. Eight estuarine-dependent fish species accounted for 94% of the individual fishes collected, and three species accounted for 96% of macrocrustacean abundance. Consistent with evidence from other Gulf of Mexico estuarine studies, species richness and abundance were highest during late spring and summer, and lowest during winter and early spring. Sites near the bay supported the most individuals and species. Associations between fish abundance and environmental variables were examined with canonical correspondence analysis. The dominant gradient was associated with water depth and distance from the bay. The secondary gradient reflected seasonal variation and was associated with temperature, salinity, dissolved oxygen, and vegetation cover. At the scales examined, estuarine biota responded to seasonal variation more than spatial variation. Estuarine-dependent species dominated the fauna and were common throughout the open waters of the shallow lake during winter–early spring when water temperature and salinity were low and dissolved oxygen high. During summer–early fall, sub-optimal environmental conditions (high temperature, low DO) in upper reaches accounted for strong spatial variation in assemblage composition. Small estuarine-resident fishes and the blue crab ( Callinectes sapidus) were common in warm, shallow, vegetated inland sites during summer–fall. Estuarine-dependent species were common at deeper, more saline locations near the bay during this period. During summer, freshwater species, such as gizzard shad ( Dorosoma cepedianum) and gars ( Lepisosteus spp.), were positively associated with water depth and proximity to the bay. The distribution and abundance of fishes in MIM appear to result from the combined effects of endogenous, seasonal patterns of reproduction and migration operating on large spatial scales, and species-specific response to local environmental variation.

Variation in levels of serum inhibin B, testosterone, estradiol, luteinizing hormone, follicle-stimulating hormone, and sex hormone-binding globulin in monthly samples from healthy men during a 17-month period: possible effects of seasons.

To obtain information on the scale of the intraindividual variation in testicular hormone, blood samples for inhibin B determination were collected monthly in 27 healthy male volunteers during a 17-month period. In addition, the traditional reproductive hormones FSH, LH, testosterone, estradiol, and SHBG were measured. The intraindividual variation in inhibin B over the study period was, on the average, 10%, corresponding to the assay variation of the inhibin B assay, indicating that most of the observed day to day variation in inhibin B levels in men could be explained by assay variation. A seasonal variation was observed in LH and testosterone levels, but not in the levels of the other hormones. The seasonal variation in testosterone levels could be explained by the variation in LH levels. The seasonal variation in LH levels seemed to be related to the mean air temperature during the month before blood sampling, but not to the length of daylight or the hours of sunshine. In conclusion, our data showed that day to day levels of inhibin B are relatively constant in men and do not seem to be influenced by seasonal factors. In contrast, we found a seasonal variation in LH and testosterone levels in men. The peak levels of both LH and testosterone were observed during June-July, with minimum levels present during winter-early spring. Air temperature, rather than light exposure, seems to be a possible climatic variable explaining the seasonal variation in LH levels.

Historical changes in lake ice‐out dates as indicators of climate change in New England, 1850–2000

AbstractVarious studies have shown that changes over time in spring ice‐out dates can be used as indicators of climate change. Ice‐out dates from 29 lakes in New England (USA) with 64 to 163 years of record were assembled and analysed for this study. Ice‐out dates have become significantly earlier in New England since the 1800s. Changes in ice‐out dates between 1850 and 2000 were 9 days and 16 days in the northern/mountainous and southern regions of New England respectively. The changes in the ice‐out data over time were very consistent within each of the two regions of New England, and more consistent than four air‐temperature records in each region. The ice‐out dates of the two regions had a different response to changes in air temperature. The inferred late winter–early spring air‐temperature warming in both regions of New England since 1850, based on linear regression analysis, was about 1.5°C. Published in 2002 by John Wiley & Sons, Ltd.

Impact of potato-cereal rotations and slurry applications on nitrate leaching and nitrogen balance in sandy soils

Groundwater quality is at risk when high levels of N fertilizers are used on sandy soils. A monitoring program was initiated in the summer of 1995, to quantify nitrate leaching in sandy soils used for potato production near Quebec city, Canada. Three drainable lysimeters were installed in each of five fields, for a total of 15 lysimeters. During a 5-yr monitoring period, crop N uptake, mineral and organic N fertilizers use, nitrate concentrations and fluxes from drainage water at 1-m soil depth were assessed under potato, cereal and hay crops. In one field, a clover and timothy sod that received low mineral N fertilizer inputs generated the lowest annual nitrate leaching losses ranging from 7 to 20 kg NO3-N ha-1. High nitrate leaching losses (116 ± 40 kg N ha-1) were measured under potato crops receiving high mineral N fertilizer inputs. Cereals, including barley and wheat receiving moderate mineral N fertilizer inputs and in some instance N from pig slurry, dairy cow manure or paper mill sludge, also generated high nitrate leaching losses (88 ± 45 kg N ha-1). Only sod and oat crops generated annual flux averaged nitrate concentrations lower than 10 mg NO3-N L-1, the accepted standard for drinking water, while higher concentrations, ranging from 13 to 52 mg NO3-N L-1, were recorded under barley, wheat and potato crops receiving moderate to high amounts of mineral N fertilizer. Nitrate flux concentrations were moderate during the cropping season (May-August), highest in fall (September-December) and lowest in the winter-early spring period (January-April). After 5 yr of survey, use of pig slurry and paper mill sludge in potato-cereal crop rotations (51 to 192 kg N ha-1 annually) with mineral N fertilizers (103 to 119 kg N ha-1 annually) resulted in nitrate leaching losses (87 to 132 kg N ha-1 annually), at least 20 kg N ha-1 more than N exported by crop at harvest. More than 60% of N applied as pig slurry seemed to be unaccounted for in the partial N balance that included crop N uptake and nitrate leaching, suggesting that important losses probably occurred through ammonia volatilization, denitrification, or N immobilization in soil organic matter and crop residues. Key words: Barley, lysimeter, nitrate leaching, nitrogen balance, pig slurry, potato

Biology and population dynamics of the long-finned squid Loligo plei (Cephalopoda: Loliginidae) in southern Brazilian waters

Population structure and reproductive patterns of the long-finned squid Loligo plei off the coast of Santa Catarina, southern Brazil, were analysed from the biological samples of commercial catches landed in the Itajaı́ harbour from April 1995 to February 1998. The species were recruited to the trawl fishery at 130 mm mantle length (ML) and males were, on average, nearly 1.3 times larger than females. The exponents of males and females ML–weight relationships were 1.89 and 2.07, respectively, and differed significantly between maturity stages. Cohorts of both sexes did not persist into their second year, a feature that, coupled with the scarcity of spent animals, provided evidence for semelparity with nearly 1 year life span. An extended seasonal breeding and spawning pattern was characterised with a large reproduction event concentrated during the summer months extending through autumn and winter. It is suggested that the summer spawners hatch during the summer–early autumn, recruit to the fishery in the spring and reach maturity in the following summer, at the age of nearly 1 year. Squid spawned during the winter will hatch during winter–early spring, recruit to the fishery during the summer and autumn and spawn in their second winter. Between summer and winter spawning events, continuous spawning activity produce several microcohorts detected in trawlers catch. Summer spawners sustain the most important commercial concentrations. The relationship between L. plei population structure, seasonal abundance and the dynamics of environmental conditions off southern Brazil is discussed.

Habitat Use by Striped Bass in Relation to Seasonal Changes in Water Quality in a Southern Reservoir

Adult striped bass Morone saxatilis (n = 61; 597-914 mm total length) were captured by hook and line throughout Lake Murray, South Carolina, and by electrofishing in the Greenwood Dam tailrace and upper Saluda River above Lake Murray, implanted with temperature-sensitive radio transmitters, and tracked biweekly. During late winter-early spring, striped bass were concentrated in the upstream portions of the reservoir. By midsummer, they were primarily located in the lower embayment of the reservoir, but several fish remained in the tailrace of the upstream dam as well as in a thermal refuge in the Saluda River. After the reservoir began to cool in fall, fish dispersed from the lower embayment and moved upstream toward the headwaters of the reservoir, where they had been captured the previous spring. Several fish returned to locations within 10 m of their original capture locations. Mean movement rates were lowest in winter and summer and highest in spring and fall. Low movement rates in summer were associated with a severe reduction of suitable habitat. In addition to the standard biweekly sampling, a 7.5-km2 section of the lower embayment of Lake Murray was searched every 2 h over a continuous 48-h period from 10 to 12 August 2000. During this period, striped bass were observed to use the same areas on a seasonal basis as they did on a diel basis. However, mean hourly rates of movement were greater than the movement rates calculated for the normal 2-week interval between samples. Changes in location between biweekly samples may not indicate displacement but rather only randomly chosen locations in normal use areas.

Component community of larval trematodes in the mudsnail Hydrobia ventrosa: temporal variations in prevalence in relation to host life history.

Temporal variations in the prevalence of larval trematodes in the short-lived prosobranch mudsnail Hydrobia ventrosa (Montagu) were investigated in relation to host life history and season for 4 successive years in temperate windflats of the southern Baltic Sea. The component community of trematode larvae in H. ventrosa comprises at least 10 species; families (and species) represented include Notocotylidae (1), Echinostomatidae (1 or 2), Heterophyidae (2), Monorchidae (1), Microphallidae (3 or 4), Psilostomatidae (1), and Hemiuridae (1). The notocotylid Paramonostomum alveatum was the most prevalent species, followed by the microphallids Maritrema subdolum and Microphallus sp. Trematode prevalence in H. ventrosa fluctuated seasonally. Prevalence usually peaked in summer between July and September-October and decreased in late winter-early spring. This seasonal change is chiefly explained by the life history patterns of the semelparous snail host. Hydrobia ventrosa has a maximum life span of about 2 yr and reproduces between June and November of its second calendar year. The first trematode infections appeared annually in May when the most abundant cohort of H. ventrosa, the second-calendar-year snails, mature. The prevalence continued to increase until August-September, throughout the reproductive period of the second-calendar-year snails, Prevalence decreased during winter, when most of the second-calendar-year snails died after reproduction. On the basis of longterm laboratory experiments, it has been shown that the late autumn-winter mortality was not the result of trematode infections. Seasonal patterns of prevalence were similar among the trematode species except for the monorchid Asymphylodora demeli, the only one using fish definitive hosts. Species-specific differences in the seasonal occurrence of prepatent infections and the predominance of certain larval stages in winter are interpreted as different strategies of the trematode species to survive the harsh winter conditions, or to survive the death of the first intermediate host in autumn-winter, or both.

Fine-fraction carbonate stable isotopes as indicators of seasonal shallow mixed-layer paleohydrography

We have measured stable isotopic compositions of Miocene pelagic fine-fraction (<63 μm) carbonates from oligotrophic deep-sea sites in the Pacific and Atlantic oceans and compared them with those of coexisting foraminifers to test their utility as near sea-surface indicators. Fine-fraction carbonates (primarily polyspecific nannofossils) and surface-dwelling planktic foraminiferal ( Globigerinoides) stable isotopes both have been considered to reflect surface-water hydrographic conditions. However, our results indicate that fine-fraction stable isotopes are offset from and do not correlate well with those of Globigerinoides. In contrast, stable isotopic records of the deep-dwelling planktic foraminifer Globoquadrina are in good correspondence with the fine-fraction records in terms of long-term (ca. >1 m.y.) trends and temporal variability. On the basis of a time-series hydrography and flux study site in the oligotrophic subtropical North Atlantic, we interpret the isotopic discrepancies between fine-fraction and Globigerinoides as resulting primarily from season of calcification, as well as possible vital effects. We suggest that fine-fraction stable isotope values from oligotrophic waters reflect late winter–early spring relatively cool, nutrient-rich shallow mixed-layer conditions during the time of deep mixing (i.e., spring bloom), whereas Globigerinoides stable isotope values record conditions that prevailed in the stratified surface waters in the warmer late spring–fall. This implies that paired analyses of fine-fraction and surface-dwelling planktic foraminiferal δ 18O could be applied to reconstruct paleoseasonality of the open oceans. However, because the fine-fraction δ 13C values are not representative of the annual mean surface-water δ 13C, we recommend use of near surface-dwelling planktic foraminiferal δ 13C as a proxy for δ 13C of stratified surface waters that are more or less in equilibrium with the atmosphere with respect to pCO 2.

The role of mesoscale processes controlling biological variability in the Black Sea coastal waters: inferences from SeaWIFS-derived surface chlorophyll field

Several different time series of chlorophyll images from the 1997–2000 Seaviewing Wide Field-of-view Sensor data set have been analysed to gain a perspective on the dynamical and biological variability in the Black Sea, particularly on its northwestern shelf region and along the Anatolian coastal zone. The images are interpreted in terms of documenting the close link between biological production and physical dynamics of the boundary system, and of emphasizing the role of eddy processes on controlling mesoscale chlorophyll distributions. It is shown that western coastal waters of the Black Sea are characterized by permanently high chlorophyll concentrations of more than 4.0 mg m −3, often subject to considerable dynamical transformations, and modulated by mesoscale structures including southward elongated filament-like features extending up to 100 km offshore with a lifetime of up to a month. Filaments, meanders, offshore jets and other forms of mesoscale and sub-mesoscale structures appear as a common signature of the system along the Anatolian coast. They play an important role in the cross-stream transport of biota and chemical constituents, and thus supporting productivity within the interior parts of the basin. The images reveal phytoplankton blooms lasting several months in all three autumn seasons. During autumn 1999 to winter 2000, the phytoplankton biomass declines in December, and then increases to form another bloom in February–March 2000, albeit somewhat weaker. For the previous 2 years (1997, 1998), the autumn bloom is extended towards the first half of the winter but the late winter–early spring bloom is absent.

Microfabric analysis of Mn-carbonate laminae deposition and Mn-sulfide formation in the Gotland Deep, Baltic Sea

The manganese carbonate deposits of the anoxic Littorina sediments of the Gotland Deep have been commonly related to the periodic renewal of deep water by inflowing saline water from the North Sea. The use of scanning electron microscopy-based techniques allows identification of small-scale sedimentary and geochemical features associated with Mn-carbonate laminae, which has significant implications for models of Mn-carbonate formation. Varves occurring in the Littorina sequence contain up to four laminae that may be placed in a seasonal cycle, and kutnahorite laminae occur within varves only as a winter-early spring deposit. This kutnahorite laminae seasonality is in agreement with the seasonal distribution of major Baltic inflow events recorded in historical records, and a direct causal link between inflows and kutnahorite deposition is implied. Benthic foraminifera tests are found to be heavily encrusted in kutnahorite, implying that benthic recolonization during oxidation events occurs concurrently with kutnahorite formation. The relatively common occurrence of small (50 to 100 μm) hexagonal γ-Mn-sulfide pseudomorphs, associated with 13% of kutnahorite laminae studied, is reported in Gotland Deep sediments for the first time. Although Mn-sulfide crystals are not usually preserved in the sediment, the discovery of Mn-sulfide pseudomorphs suggests that initial formation of Mn-sulfide in the Gotland Deep may occur much more commonly during the process of kutnahorite formation than previous reports of Mn-sulfide occurrence have implied.

Major outputs of the recent multidisciplinary biogeochemical researches undertaken in the Aegean Sea

The main outputs of a multidisciplinary and integrated studies are summarised. The results incorporate the latest biogeochemical researches, at basin scale, in the Aegean Sea (including thermohaline circulation studies, SPM dynamics, mass and energy fluxes, acknowledge biochemical processes in the euphotic and the benthic layer and benthic response to downward fluxes). The data were acquired within five (seasonal) research cruises, during 1997–1998. Data analysis and evaluation hence provided important new information on the functional processes of the Aegean ecosystem. In terms of water circulation, no new deep water formation in the Aegean Sea was observed, during 1997–1998, but rather intermediate water, due mainly to the mild winter conditions. All the biochemical parameters of the euphotic zone (nutrients, Particulate Organic Carbon (POC), chlorophyll- a, phytoplankton, primary and bacterial production), although high in the N. Aegean Sea reflect clearly the highly oligotrophic character of the Aegean Sea. In the N. Aegean, microbial food web was the main pathway of carbon, whereas in the S. Aegean, the food web could be classified as multivorous. An important Black Sea Water (BSW) signal was observed in the dissolved phase; this was especially pronounced in the Dissolved Organic Carbon (DOC), Mn and to a lesser degree to Cd, Cu and Ni concentrations. The downward material fluxes are higher in the N. Aegean, relative to the S. Aegean. Substantially higher values of near-bottom mass fluxes were measured in the deep basins of the N. Aegean, implying significant deep lateral fluxes of POM. The N. Aegean could be classified as a “continental margin” ecosystem, whilst the S. Aegean is a typical “oceanic margin” environment. There is a close relationship and, consequently, coupling between the near-bottom mass fluxes and the accumulation rates of organic matter (OM), with the near-bottom mineralisation, bioturbation, redox potential, oxygen consumption rates, the sediment microbiological and enzymatic activity and the meio-macro- and mega fauna abundances in the Aegean Sea. The N. Aegean is characterised by higher fluxes of labile POC and higher rates of benthic mineralisation and displays much higher benthic productivity and diversity relatively to the S. Aegean. The deep isolated N. Aegean basins should be regarded as “hot spots” of organic matter accumulation, benthic abundances and diversity. There is a general N–S gradient in the biogeochemical and benthic processes in the Aegean that is especially pronounced during winter–early spring, implied mainly by the of Black Sea Water (BSW) inflow (through the dissolved phase) and the lateral marginal inputs.

Seasonal Variation in Sex Ratio and Asynchronous Presence at Ponds of Male and Female Triturus Newts

Adult Triturus newts show spatial and temporal variation in sex ratio at the aquatic breeding site, to the extent that contrasting scenarios on the evolution of mating systems appear to be supported. I collected data for the five western European species (Triturus alpestris, Triturus cristatus, Triturus helveticus, Triturus marmoratus, and Triturus vulgaris) over 500 ponds and 27 yr in two areas in France, to test the hypothesis that the phenology of pond presence is independent of gender. Approximately equal numbers of males and females were found. However, in T. marmoratus males outnumbered females, which could be explained by a proportion of females skipping annual breeding opportunities. In the hybrid T. cristatus × T. marmoratus, females were twice as numerous as males, in accordance with Haldane's rule. A trend was observed for a male-biased sex ratio at the start of the aquatic season (late winter–early spring) and for a female bias toward the end of the aquatic season (early summer), suggesting that males mostly arrive and leave the breeding sites ahead of the females. The data do not support evolutionary scenarios selecting for the early breeding of female T. vulgaris.

The long-term impact of nitrogen fertiliser on perennial ryegrass tiller and white clover growing point densities in grazed dairy pastures in south-western Victoria

A 3-year grazing experiment determined the impact of multiple applications of different rates of nitrogen (N) fertiliser, applied over autumn and winter in 1997, 1998, and 1999, on perennial ryegrass (Lolium perenne)/white clover (Trifolium repens) tiller and growing point densities (stolon apices with at least 2 nodes). Annual pasture dry matter (DM) yields were also monitored. Four treatments were replicated 3 times in a randomised block design and included: 0 N (A); 3 applications of 25 kg N/ha (B); 3 applications of 50 kg N/ha (C); and 3 applications of 75 kg N/ha (D). Urea (46% N) was the N source. Grazing of treatment plots occurred at a pre-grazing herbage mass of 2200–2500 kg DM/ha.Over 3 years, N applications consistently increased annual pasture DM yields by 0.9–3.3 t/ha when a total of 75–225 kg N/ha was applied annually.Generally, treatments B, C, and D resulted in higher perennial ryegrass tiller densities than treatment A. An exception occurred from July 1998 in Year 2 to July 1999 in Year 3, when all perennial ryegrass densities were similar. Nitrogen fertiliser generally produced no consistent effect on white clover growing point density, with the exception of July–December in Year 2 when treatments B, C, and D resulted in lower growing point densities than treatment A. Clover growing point density decreased over the trial period irrespective of treatment. There were no N fertiliser effects on 'other' grasses and broadleaved weeds. 'Other' grasses (mainly winter grass, Poa annua) did, however, peak in density (up to 2500 tillers/m2) from July to September each year.Seasonally, the peak perennial ryegrass tiller density was similar each year and occurred during late winter–early spring (5450 tillers/m2 in July 1997; 6200 tillers/m2 in August 1998; 5400 tillers/m2 in July 1999). This was followed by a trough over midsummer (800 tillers/m2 in January 1998; 725 tillers/m2 in January 1999). White clover growing point density declined over 3 years. During this decline there were peaks in June 1997 (2650 growing points/m2), November 1997 (1600 growing points/m2), June 1998 (1250 growing points/m2), April 1999 (1050 growing points/m2), and November 1999 (850 growing points/m2). Troughs occurred in January 1998 (530 growing points/m2) and February 1999 (380 growing points/m2).It is concluded that although increasing applications of N increased annual pasture DM yields and generally increased perennial ryegrass tiller densities, with little effect on clover growing point densities, there is little to suggest that N fertiliser alone would enhance the persistence of these pasture species. Persistence is likely to be influenced by a combination of factors including grazing management and climatic effects, rather than N fertiliser alone.

Particle Transport, Nutrient Cycling, and Algal Community Structure Associated with a Major Winter-Spring Sediment Resuspension Event in Southern Lake Michigan

Over the past decade, intermittent satellite imagery revealed the presence of an extensive plume of resuspended sediments in late winter-early spring with a clear offshore projection coinciding with the region of maximum sediment accumulation in the lake. The large scale of the plume implied that this process was important in sediment, and associated constituent, cycling and transport, but it had never been sampled due to severe conditions. The onset of the 1996 event coincided with a major March storm. Within a few days the plume was approximately 10 km wide and over 300 km in length, implying that the source of the reflective materials was widely distributed. An estimate of the total mass of resuspended sediment, 12 days after the storm, was similar to the annual external load of (sand-free) particulate material to the southern basin. The high turbidity plume persisted for over a month, progressing northward along the eastern shore with a major offshore transport feature. Sediment traps within this feature recorded a major mass flux event. The plume was sampled on two occasions and was found to contain 5 to 10 times as much suspended matter as open-lake locations outside the visible plume. Total particulate phosphorus was high within the plume making this episodic process important in sedimentwater exchange. The diatom community structure within the plume was significantly different from outside the plume and was characteristic of more eutrophic waters. Abundance of non-diatom phytoplankton and microbial food web organisms were highest at the plume edge. The episodic nature of this process makes it difficult to sample, but the scale makes it important in designing monitoring programs and massbalance modeling efforts.