Background and Study Aim. The objective of the study was to perform a systematic review of the literature and meta-analysis to determine the validity of running-based sprint test in relation to 30 second Wingate anaerobic test.
 Material and Methods. A search of the relevant literature was done using the key words, ‘running-based anaerobic sprint test’, ‘RAST’, ‘Validity’ ‘repeated sprint’ and ‘Wingate’. Twelve studies including 368 participants were finalized to systematic review and meta-analysis. The mean ± standard deviation of the number of participants was 30.66 ± 16.17 years.
 Results. The summary of effects size were calculated to established the validity of running based sprint test (RAST) with 30 seconds Wingate test as a criterion measure. All studies indicate that effect size of Peak Power (PP) shows higher summary effects 0.58 (95%CI – 0.37, 0.79), similar outputs were observed for Mean Power (MP) 0.67 (95%CI – 0.45, 0.90). Therefore, the average outcomes were significantly different from zero.
 Conclusions. Running-based anaerobic sprint test is a valid alternative method of 30 seconds Wingate test to measure anaerobic power outputs of healthy individual belongs to various sports disciplines. Although, anaerobic capacity or power output is a determinant factor in power dominating sports. Therefore, RAST is compatible to laboratory-based Wingate 30 second anaerobic test (WAnT) in field-based settings.