Wing Shape Research Articles

A geometric morphometrics and genetics characterization of Vanessa carye in an extreme elevational gradient in the Chilean Altiplano

The Western Painted Lady butterfly, Vanessa carye (Hüber, 1812), in addition to pre-senting an extensive distribution in South America, has been observed flying in the Chilean Alti-plano from the coast to the highlands, from 0 to 5200 m.a.s.l. and it is suggested that could be a migratory insect. Some animal species move from one place to another by migration searching for favorable conditions. Wings of flying migratory animals are the principal trait involved in move-ment, and their shape has been documented to change between migratory and non-migratory species or populations. In this sense, little is known about the adaptations of butterflies to elevational gradients, so the V. carye's wide latitudinal and altitudinal range coupled with the different climatic conditions makes this species an interesting subject for adaptation studies. This research studies V. carye at different elevational ranges in extreme environments at the Chilean Altiplano and Atacama Desert to determine the presence of morphotypes through geometric morphometrics associated with altitudinal patterns, and to determine the genetic diversity and population structure of this species by using COI molecular marker. Subtle morphometric changes between populations were found, so two morphometric lineages were defined: a “coastal” lineage with more elongated wings and an “altiplano” lineage with more rounded wings. Nevertheless, low genetic diversity and no genetic structure associated to these morphological differences were found. Understanding the processes underlying the wing shape of V. carye morphological adaptations will help to understand its natural history. Results here may be a first approach of evidence to answer the question “Is this butterfly a migratory species?”

A review on insects flight aerodynamics, noise sources, and flow control mechanisms

Wildlife always acts as an inspiration source for humans to help them study and mimic flight methods. Insects are one of the most important sources of biological systems’ inspiration to control flow and reduce aerodynamic noise. Insects are classified into different kinds, and most can fly by fluttering their wings. In general, insects’ flight muscles are divided into direct and indirect types that act synchronously and asynchronously with nerve impulses, respectively. These muscles help insects use a mixture of rotating, flapping, and pitching movements to achieve specific wing kinematics. Insects use various mechanisms for generating aerodynamic forces, including the Weis-Fogh or clap and fling mechanism, delayed stall due to unsteady motion (Wagner effect), wing rotation (Kramer effect), wake capture or wing–wake interaction, added mass, and absence of stall. On the other hand, the insect noises are divided into aerodynamic and structural. Insects’ aerodynamic noise is created by fluctuating forces, flow–solid interaction, shed vortex, and turbulence inflow. Meanwhile, insects’ structural noise is made by frictional and tymbal mechanisms. Their flow control methods are classified into two categories: wing shape and sub-structures. Wing shape features such as planform, chord length and location, twist, sweep, wingtip, and aspect ratio influence the flow around the insects. The sub-structures such as leading edge, trailing edge, swallowtail, and surface textures affect the flow too. A thorough understanding of insects’ fly, aerodynamic noise, and their control flow techniques will significantly help engineers to produce competitive products with better aerodynamic performance and aeroacoustic signature.

Larval performance and adult phenotypic variation of Acraea terpsicore (Lepidoptera: Nymphalidae) on congeneric Passiflora host plants.

The introduction of exotic plants into different habitats can have varied consequences for local herbivorous insects, such as altered the nutrient profiles that induce the host plant shifting or reduced food availability by competition with native plants. Acraea terpsicore (Linnaeus, 1758), a common butterfly in Southeast Asia that uses Passiflora as a host plant, has been studied to compare larval performance and adult phenotypes on one local wild Passiflora plant, P. foetida and 3 exotic congeneric plants, P. edulis, a fruit crop plant, and P. x alata-caerulea and P. x coccinea-caerulea, both hybrid ornamental plants. Laboratory caterpillars that fed on P. edulis and P. x alata-caerulea died within 1-10 days, while caterpillars fed on P. foetida and P. x coccinea-caerulea completed their life cycle in 30.42 ± 0.25 days. Although the caterpillars differed in several food utilization indices, the relative growth rate and consumption index were not affected. Adult butterflies that hatched from caterpillars fed on these experimental plants had different phenotypes, especially wing colors. Therefore, although some congeneric exotic plants may have a negative impact on the butterfly population, some exotic plants can serve as alternative larval host plants and expand the ecological niche of butterflies. Difference in the composition and concentration of nutrients and defensive chemicals in each host plant may exert an impact on the phenotypic traits of butterflies, such as their body size, coloration, and wing shape. Whether butterflies may separate into different subpopulations in the future due to phenotypic variation should be further examined.

Correction to: Wing size, shape, and asymmetry analysis of the wandering glider, Pantala flavescens (Odonata: Libellulidae) revealed that hindwings are more asymmetric than the forewings

Correction to: Wing size, shape, and asymmetry analysis of the wandering glider, Pantala flavescens (Odonata: Libellulidae) revealed that hindwings are more asymmetric than the forewings

Wing dynamic shape-sensing from fiber-optic strain data using the Kalman state estimator

A novel method for shape sensing based on strain data and the use of the Kalman state estimator is presented and studied experimentally. Several recent studies proposed different methods in which strain data, measured in optical fibers, can be used to estimate the deformed shape of a flexible wing. Fiber optic sensors typically provide accurate, high resolution, strain data, yielding detailed prediction of a wing's deformed shape. However, in real life applications there could be moments in which the strain data is imperfect (for example, due to saturation or temperature effects), corrupted, or missing. The current study proposes to use a Kalman state estimator that weighs in strain-data and simulation output of an aeroelastic plant model to estimate the wing deformations. The method is demonstrated with a flexible wing model that is excited by prescribed control surfaces deflection in a wind-tunnel test. Strains over the front and rear wing spars are measured by Fiber Bragg Grating sensors, embedded in two optical fibers, and used to estimate the wing deformations. The latter are compared to wing deformations measured by a motion recovery camera system. Results show the advantages of the use of the Kalman state estimator when the strain data is corrupted. Additionally, with this approach, wing's modal velocities are estimated together with the wing deformations, resulting in smooth and accurate modal velocities that are readily usable by the vehicle's control system.

Optimization of Wing Rib Configurations for Improved Aerodynamic Performance: A Computational Study

Abstract: This research paper presents a computational study on the optimization of wing ribs configurations for improved aerodynamic performance. The wing ribs play a crucial role in supporting the wing's shape and contour, and their design is critical for achieving optimal flight performance. An aircraft's wing is a complex structure because of the complicated way it responds to different loads and manoeuvres. The main objective of this study is to develop a more efficient and optimized rib structure that can improve the aerodynamic performance of the wing, specifically by reducing drag and increasing lift. The study uses computational fluid dynamics (CFD) simulations to analyze the aerodynamic performance of different wing rib configurations. The simulations are performed on a representative wing model, and different rib designs are evaluated to determine their impact on the overall aerodynamic performance. The results of the study demonstrate that the optimized rib structure can significantly improve the aerodynamic performance of the wing, with a reduction in drag and an increase in lift. The study also highlights the potential benefits of using computational techniques for designing and optimizing aircraft components, such as reducing design time and cost, improving accuracy, and enabling rapid prototyping. The findings of this study are expected to contribute to the development of more efficient and effective aircraft design processes, benefiting both the aviation sector and the public.

Wing size, shape, and asymmetry analysis of the wandering glider, Pantala flavescens (Odonata: Libellulidae) revealed that hindwings are more asymmetric than the forewings

Wing size, shape, and asymmetry analysis of the wandering glider, Pantala flavescens (Odonata: Libellulidae) revealed that hindwings are more asymmetric than the forewings

Multidisciplinary Design and Optimization of Variable Camber Wing with Non-Equal Chord

Since the taper ratio of most wings is not equal to 1, the beam-disk trailing edge deflection mechanism originally designed for the rectangular wing is not fully applicable to the non-equal chord wing. Moreover, it is not only expected that the wing shape can achieve excellent aerodynamic performance under different flight conditions, but one also needs to consider whether the flexible skin can achieve this deformation. This paper used the honeycomb composite structure with zero Poisson’s ratio as the flexible skin of the trailing edge for the variable camber wing, and designed the beam-disk trailing edge deflection mechanism for the non-equal chord wing. The aerodynamic configuration was optimized considering the deformation capability of the skin, and the multidisciplinary design and optimization method of the variable camber wing with non-equal chord was studied. The results show that the aerodynamic performances of the optimized non-equal chord wings were better than before under all given flight conditions. The flexible skin could withstand the strain caused by the maximum deflection of the trailing edge of the wing, and the weight of the wing structure was reduced by 47.1% compared with the initial design when the structural stiffness and strength were satisfied.

Complexities of recapitulating polygenic effects in natural populations: replication of genetic effects on wing shape in artificially selected and wild-caught populations of Drosophila melanogaster.

Identifying the genetic architecture of complex traits is important to many geneticists, including those interested in human disease, plant and animal breeding, and evolutionary genetics. Advances in sequencing technology and statistical methods for genome-wide association studies have allowed for the identification of more variants with smaller effect sizes, however, many of these identified polymorphisms fail to be replicated in subsequent studies. In addition to sampling variation, this failure to replicate reflects the complexities introduced by factors including environmental variation, genetic background, and differences in allele frequencies among populations. Using Drosophila melanogaster wing shape, we ask if we can replicate allelic effects of polymorphisms first identified in a genome-wide association studies in three genes: dachsous, extra-macrochaete, and neuralized, using artificial selection in the lab, and bulk segregant mapping in natural populations. We demonstrate that multivariate wing shape changes associated with these genes are aligned with major axes of phenotypic and genetic variation in natural populations. Following seven generations of artificial selection along the dachsous shape change vector, we observe genetic differentiation of variants in dachsous and genomic regions containing other genes in the hippo signaling pathway. This suggests a shared direction of effects within a developmental network. We also performed artificial selection with the extra-macrochaete shape change vector, which is not a part of the hippo signaling network, but showed a largely shared direction of effects. The response to selection along the emc vector was similar to that of dachsous, suggesting that the available genetic diversity of a population, summarized by the genetic (co)variance matrix (G), influenced alleles captured by selection. Despite the success with artificial selection, bulk segregant analysis using natural populations did not detect these same variants, likely due to the contribution of environmental variation and low minor allele frequencies, coupled with small effect sizes of the contributing variants.

Geometric Morphometric Analysis of Wing Shape to Identify Populations of Apis mellifera in Camagüey, Cuba.

A total of 45 Apis mellifera colonies were sampled from nine centers for rearing queens in the Camagüey province, Cuba. Wing geometric morphometric analysis was used to determine the ancestry and identify Africanization processes at different altitudes in managed honeybee populations on the island. A total of 350 reference wings were obtained from the pure subspecies: Apis mellifera mellifera, Apis mellifera carnica, Apis mellifera ligustica, Apis mellifera caucasia, Apis mellifera iberiensis, Apis mellifera intermissa, and Apis mellifera scutellata for the study. Our results showed that altitude influences wing shape; and that 96.0% (432) of the individuals were classified as Cuban hybrids, with a tendency to the formation of a new morphotype. In addition, a great similarity was found with the subspecies Apis mellifera mellifera, and it was confirmed that there is no Africanization due to the low presence of 0.44% (2) of this morphotype in the population under study. The greatest Mahalanobis distances were obtained for the comparisons between the center rearing of queens in the Camagüey province with the subspecies A. m. scutellata (D2 = 5.18); A. m. caucasia (D2 = 6.08); A. m. ligustica (D2 = 6.27); and A. m. carnica (D2 = 6.62). The well-defined pattern of wing shape produced by honeybee populations in Camagüey's centers for queen rearing suggests a Cuban hybrid. Moreover, it is essential to note that the populations of bees under investigation lack Africanized morphotypes, indicating that Camagüey bees have not interacted with the African lineage.

The relationship between the distribution of facial erythema and skin type in rosacea patients: a cross-sectional analysis.

Individuals with rosacea have different facial erythema distribution patterns; however, whether they are related to the skin type is unclear. This study enrolled 201 Chinese patients, including 195 females and six males, diagnosed with rosacea. Facial images were taken using the VISIA® Complexion Analysis System, and red-area images were further analyzed. The erythema distribution pattern of rosacea was divided into peace signs, wing shapes, and neither of the two patterns, according to the distribution location. Skin types were divided according to the Fitzpatrick skin type, and oily-dry skin subtypes were determined according to the Baumann skin-type scale. There were 130 and 44 cases of typical peace signs and typical wing shapes, respectively. The remaining 27 cases were of neither type. Among the 76 patients with peace-sign patterns, the majority (58.5%) had oily combination skin. Among the patients with a typical wing shape, 43 (97.7%) had dry combination skin. Among the 27 patients with no peace-sign or wing-shape pattern, 17 (63.0%) had dry combination skin (p < 0.05). The peace sign pattern was more common in individuals with darker skin tones (p < 0.05). The differences in the immune microenvironment, Demodex habitation, and altered lipid content may explain the presence of the peace-sign pattern in the oily combination skin population. Wing-type patterns are associated with the lateral parts of the cheeks and could be caused by abnormal vessel dilations of the anatomic branches of the zygomatic-facial and facial arteries, which indicates that the main pathogenesis for this type of rosacea may be neurovascular. Our study is the first to report that facial erythema distribution characteristics of rosacea are associated with skin types.

Evaluating the significance of wing shapes in inferring phylogenetic proximity among the generic taxa: an example of Cantharinae (Coleoptera, Cantharidae)

The resolution of phylogenetic relationship among animals is still one of the most challenging problems in systematic zoology. Insect wing is a highly valued morphological character in the systematics, but few studies have been conducted to quantify wing shape variations for phylogenetic reconstruction. In this study, with Cantharinae as the subject, we conducted the GM analyses from hindwings of 16 representative genera. Further, we conducted the UPGMA based on Procrustes distance and Euclidean similarity measure of Mahalanobis distance, respectively, and NJ analysis of the Mahalanobis distance, as well as MP analysis using merged landmark dataset. In the meantime, we constructed the phylogenetic relationships among these genera based on the mitochondrial genomes, with a total of 41 sequences novel to Cantharinae, by BI and ML analyses. As a result, the CVA analysis demonstrated that the hindwing shapes of the cantharid genera are significantly different from one another. All the topologies produced by the GM data partially correspond with that of mitogenomic data. The close relationships of some genera are frequently recovered, including Cyrebion + Themus, Cantharis + Taiwanocantharis + Taocantharis, Stenothemus + Falsopodabrus + Habronychus. These results prove the importance and potential application of the hindwing shapes in recovering the relationships among the sibling genera.

Fourth High-Lift Prediction/Third Geometry and Mesh Generation Workshops: Overview and Summary

The Fourth AIAA Computational Fluid Dynamics (CFD) High Lift Prediction Workshop and the Third Geometry and Mesh Generation Workshop were held collaboratively with the common goal of assessing the numerical prediction capability of current-generation computational fluid dynamics technology for swept medium-/high-aspect-ratio wings in high-lift configurations. A key aspect of this joint endeavor was the use of technology focus groups: an innovative new approach for workshops involving close collaboration between participants. These groups, which included both mesh-generation and flow-solver experts, worked to accelerate advancements for their particular methodologies by addressing key questions of importance before the workshop. The high-lift version of the NASA Common Research Model (CRM-HL) configuration was the focus of this workshop. Measured experimental wind-tunnel data were available for comparison. The workshop also included a two-dimensional turbulence model verification exercise based on the CRM-HL wing shape. Altogether, 44 participants submitted a total of 184 datasets of CFD results. This paper provides a high-level summary of the results and conclusions from the workshop. Like at past workshops, fixed-grid Reynolds-averaged Navier–Stokes methods continued to be inaccurate and inconsistent for predicting high-lift flow physics near maximum lift conditions. However, mesh adaptation definitively brought more consistency. Scale-resolving methods appeared most promising for predicting high-lift flow physics near maximum lift.

Optimization Design of the Wing of Cruise Missile on the Basis of the Vortex Lattice Approach

Aerodynamic performance of missile wings is a vital factor affecting the endurance and combat effectiveness of cruise missiles. Given the current situation that the aerodynamic design of conventional cruise missile wings is less and there is more room for improvement, a missile wing shape with a winglet is considered designed to improve the cruise characteristics of cruise missiles effectively. Firstly, the EPPLER 66 is selected as the wing shape by the combination of vortex surface element approach batch calculation and CFD simulation analysis. According to the dimensional parameters of a typical patrol wing, the design variables and corresponding optimization intervals are determined, and the aerodynamic optimization is carried out by combining the genetic approach as well as the vortex lattice approach. CFD is used to verify the aerodynamic performance of the optimized wing. The calculation outcomes reveal that the optimized wing has a ratio of lift-to-drag of 23.43, which is 12.32% higher than that of the conventional flat wing.

Species Discrimination of Stomoxys Flies S. bengalensis, S. calcitrans, and S. sitiens (Diptera: Muscidae) Using Wing Geometric Morphometrics.

The flies of the genus Stomoxys Geoffroy, 1762 (Diptera: Muscidae), are regarded as pests of veterinary and medical importance. In Thailand, Stomoxys calcitrans (Linnaeus, 1758) is the most abundant species and is widely distributed throughout the country. This Stomoxys species can coexist with two other morphologically similar species: Stomoxys bengalensis Picard, 1908, and Stomoxys sitiens Rondani, 1873. Hence, discriminating using morphological characteristics is difficult, especially if the specimen is damaged or loses its diagnostic characteristics. In this study, we evaluated the effectiveness of the landmark-based geometric morphometric (GM) approach to discriminate among the three Stomoxys spp.: S. bengalensis, S. calcitrans, and S. sitiens. Left-wing images of S. bengalensis (n = 120), S. calcitrans (n = 150), and S. sitiens (n = 155) were used for the GM analyses. The results of the wing shape analyses revealed that the GM approach was highly effective for discriminating three Stomoxys, with high accuracy scores ranging from 93.75% to 100%. This study adds to the evidence that landmark-based GM is an excellent alternative approach for discriminating Stomoxys species.

Modeling study of the impact of actomyosin contractility on cell proliferation in the Drosophila wing imaginal disc.

A fundamental open question in developmental biology is deciphering how the regulation of cell proliferation and the actomyosin cytoskeleton coordinate proper formation and maintenance of tissue shape and size. To address this question, a close integration between experimental work and simulations from a novel multi-scale computational model are used. Specifically, a subcellular element (SCE) model of the Drosophila wing imaginal disc cross-section along the anterior-posterior axis is developed and calibrated using experimental data. This model accounts for actomyosin contractility in different cell regions and incorporates a detailed cell division sub model capable of capturing interkinetic nuclear migration, a fundamental process for proper cell division. Coupled model simulations and experiments reveal that anisotropic patterning of actomyosin contractility can regulate basal local curvature of the wing disc and positioning of nuclei. Moreover, it was found that cell proliferation also plays an important role in regulating basal curvature. However, this model has a simplified representation of actomyosin contractility which is not able to capture the micro level interactions between actin filaments and myosin motors that make up the actomyosin network and thus, the direction of the contractile forces cannot be accounted for explicitly. Therefore, in order to have a more biological representation of actomyosin, a coarse-grained stochastic model of subcellular level actomyosin network dynamics in two-dimensions is being developed. This model describes the actomyosin network via a large system of nodes with their dynamics represented by stochastic ordinary differential equations. The multi-scale SCE model, coupled with the stochastic model, will be used to gain insight into the detailed mechanism of how actomyosin contractility facilitates interkinetic nuclear migration, cell proliferation and formation of the wing shape.

Species Identification of the Major Japanese Encephalitis Vectors within the Culex vishnui Subgroup (Diptera: Culicidae) in Thailand Using Geometric Morphometrics and DNA Barcoding

Japanese encephalitis (JE) is a viral infection of the brain caused by the Japanese encephalitis virus, which spreads globally, particularly in 24 countries of Southeast Asia and the Western Pacific region. In Thailand, the primary vectors of JE are Cx. pseudovishnui, Cx. tritaeniorhynchus, and Cx. vishnui of the Cx. vishnui subgroup. The morphologies of three mosquito species are extremely similar, making identification challenging. Thus, geometric morphometrics (GM) and DNA barcoding were applied for species identification. The results of cross-validation reclassification revealed that the GM technique based on wing shape analysis had relatively high potential for distinguishing Cx. pseudovishnui, Cx. tritaeniorhynchus, and Cx. vishnui (total performance = 88.34% of correctly assigned individuals). While the DNA barcoding yielded excellent results in identifying these Culex species based on the DNA barcode gap (average intraspecific genetic distance = 0.78% ± 0.39% and average interspecific genetic distance = 6.14% ± 0.79%). However, in the absence of the required facilities for DNA barcoding, GM techniques can be employed in conjunction with morphological methods to enhance the reliability of species identification. Based on the results of this study, our approach can help guide efforts to identify members of the Cx. vishnui subgroup, which will be useful for the effective vector control of JE in Thailand.

Species morphospace boundary revisited through wing phenotypic variations of Antodynerus species (Hymenoptera: Vespidae: Eumeninae) from the Indian subcontinent

The main objective of this study was to investigate the taxonomic significance of wing phenotypic variations (size and shape) for classifying potter wasps. This is the first study investigating the wing size and shape variations, as well as wing asymmetry, sexual dimorphism, wing integration, and phylogenetic signal analysis of all known Antodynerus species from the Indian subcontinent: A. flavescens, A. limbatus, and A. punctatipennis. We used forewings and hindwings for geometric morphometric analysis, and we proved that each species’ wing had unique size and shape variations, as well as significant right–left wing asymmetry and sexual dimorphism across the Antodynerus species, as verified by discriminant function analysis. Wings of Vespidae are longitudinally folded; based on that, we tested two alternative wing modular hypotheses for evaluating the wing integration, using two subsets organization, such as anterior–posterior (AP) and proximal-distal (PD) wing modular organization. We proved that Antodynerus species wings are highly integrated units (RV &amp;gt; 0.5), and we rejected our hypothesis at p &amp;lt; 0.05. The morphospace distribution analysis revealed that each species has its unique morphospace boundary, although they share some level of homoplasy, which suggests to us that we can use wing morphometric traits for Antodynerus species delimitation. In addition, we revealed the phylogenetic signal of Antodynerus species. Surprisingly, we found a shape-related phylogenetic signal in the forewing, and there is no significant (p &amp;gt; 0.05) phylogenetic signal in forewing size, hindwing shape, and size. We observed that the Antodynerus species’ forewing shape is evolutionarily more highly constrained than the hindwing. We found that A. limbatus and A. flavescens with distinct geographical distribution share a similar evolutionary history, while A. punctatipennis evolved independently.

Evaluation of Modern Techniques for Species Identification of Lutzia Mosquitoes (Diptera: Culicidae) in Thailand: Geometric Morphometrics and DNA Barcoding.

There are four species of Lutzia mosquitoes in Thailand, including Lutzia chiangmaiensis, Lt. fuscana, Lt. halifaxii, and Lt. vorax. The accurate species identification of adult Lutzia mosquitoes based on morphological features requires many body parts, including the abdominal terga and wing. However, species identification is difficult in the case of damaged specimens when some of their morphological character is missing due to transit or gathering in the field. Thus, we evaluated the efficacy of the landmark-based geometric morphometric (GM) approach for the discrimination of Lutzia species in Thailand. In addition, DNA barcoding was also used in parallel with the GM approach to identify the species. Larvae of Lutzia were collected, raised into adults, and identified based on their morphological characteristics. The validated reclassification test results clearly demonstrated that wing shape resulted in a high level of success in identification (correct identifications ranged from 92.50% to 100%); however, based on the DNA barcoding analyses, our results showed that it was poorly effective in identifying Lt. fuscana and Lt. halifaxii based on an overlap between the intraspecific and interspecific divergence. Moreover, our survey results provide updates on the distribution of Lt. chiangmaiensis and Lt. vorax in Thailand. This research will help medical entomologists more efficiently identify mosquitoes in the genus Lutzia, resulting in more effective mosquito control and surveillance.

Phenotypic variability in traits related to flight dispersal in the wing dimorphic species Triatoma guasayana

BackgroundTriatoma guasayana is considered an emerging vector of Chagas disease in the Southern Cone of South America. The presence of a triatomine population with brachypterous individuals, in which both wings are reduced, has recently been reported for this species. The aim of the present study was to determine if flight-related traits varied across populations, if these traits could explain differences in flight capacity across populations and if flight-related traits are associated with geographic and/or climatic variation.MethodsThe study involved 66 male T. guasayana specimens from 10 triatomine populations. Digital images of wing, head and pronotum were used to estimate linear and geometric morphometric variables. Variations in size and shape were analysed using one-way analysis of variance and canonical variate analysis (CVA), respectively. Mantel tests were applied to analyse the relationship between morphometric and geographic distances, and the association between size measurements was analysed using Pearson’s correlation. We explored covariation between size and shape variables using partial least square analyses (PLS). The association of geographic and climatic variables with size measurements was tested using linear regression analyses. We performed PLS analyses for shape measurements.ResultsWing size differed significantly across triatomine populations. The CVA showed that wing shape of the brachypterous population is well discriminated from that of the other populations. The Mantel test showed a positive and significant association between wing shape and geographic distances. The heads of the brachypterous population were significantly larger than those of the other populations. Similar to wing shape, the head shape of the brachypterous population was well discriminated from those of the other populations. Pronotum width did not show significant differences across populations. Geographic and climatic factors were associated with size and shape of both the wing and head, but not with pronotum width.ConclusionsMost of the traits related to flight dispersal varied across populations. Wing shape and head shape were found to be better markers for differentiated morphological variation across populations. Head measurements also varied in accordance with this condition. Geographic and climatic variables were associated with most of the flight-related traits.Graphical