Window Screens Research Articles

AroER Tri-Screen Is a Biologically Relevant Assay for Endocrine Disrupting Chemicals Modulating the Activity of Aromatase and/or the Estrogen Receptor

Endocrine disrupting chemicals (EDCs) interfere with the biosynthesis, metabolism, and functions of steroid hormones, including estrogens and androgens. Aromatase enzyme converts androgen to estrogen. Thus, EDCs against aromatase significantly impact estrogen- and/or androgen-dependent functions, including the development of breast cancer. The current study aimed to develop a biologically relevant cell-based high-throughput screening assay to identify EDCs that act as aromatase inhibitors (AIs), estrogen receptor (ER) agonists, and/or ER antagonists. The AroER tri-screen assay was developed by stable transfection of ER-positive, aromatase-expressing MCF-7 breast cancer cells with an estrogen responsive element (ERE) driven luciferase reporter plasmid. The AroER tri-screen can identify: estrogenic EDCs, which increase luciferase signal without 17β-estradiol (E2); anti-estrogenic EDCs, which inhibit the E2-induced luciferase signal; and AI-like EDCs, which suppress a testosterone-induced luciferase signal. The assay was first optimized in a 96-well plate format and then miniaturized into a 1536-well plate format. The AroER tri-screen was demonstrated to be suitable for high-throughput screening in the 1536-well plate format, with a 6.9-fold signal-to-background ratio, a 5.4% coefficient of variation, and a screening window coefficient (Z-factor) of 0.78. The assay suggested that bisphenol A (BPA) functions mainly as an ER agonist. Results from screening the 446 drugs in the National Institutes of Health Clinical Collection revealed 106 compounds that modulated ER and/or aromatase activities. Among these, two AIs (bifonazole and oxiconazole) and one ER agonist (paroxetine) were confirmed through alternative aromatase and ER activity assays. These findings indicate that AroER tri-screen is a useful high-throughput screening system for identifying ER ligands and aromatase-inhibiting chemicals.

Experimental Investigation on Heating Performance of Heat Pump for Electric Vehicles in Low Ambient Temperature

In comparison with the traditional cars, Electric Vehicles (EVs) cannot utilize the waste heat of the engines to keep warm inside the car in winter. As a potential heat source, air source heat pump (ASHP) for EVs with scroll compressor and test bench has been built to investigate the heating performances on heat pump system in low ambient temperature. Based on the demand of moisture condensation control on the window screen of EVs, the heating performances are tested on both all fresh air supply and various indoor air inlet temperature conditions, under low environmental temperature -10oC, -15oC and -20oC. The experimental results show that ASHP for EVs has big differences to buildings or household ACs due to its big ratio of fresh air, even at -20oC, the system's COP under the largest heat capacity is over 1.7.

Reduced Prevalence of Malaria Infection in Children Living in Houses with Window Screening or Closed Eaves on Bioko Island, Equatorial Guinea

BackgroundPrevious studies demonstrated that fewer mosquitoes enter houses which are screened or have closed eaves. There is little evidence about the effect on malaria infection in humans that changes in house construction may have. This study examines the impact of protective housing improvements on malaria infection on Bioko Island.Methodology/Principal FindingsData from the annual malaria indicator surveys between 2009 and 2012 were used to assess trends in housing characteristics and their effect on RDT confirmed malaria infection in household members. Odds ratios were adjusted for socio-economic status of the household.22726 children between the ages of 2 and 14 years were tested for P. falciparum. Prevalence of infection in those living in houses with open eaves was 23.0% compared to 18.8% for those living in houses with closed eaves (OR = 0.81, 95% CI 0.67 - 0.98). The prevalence of infection for children in screened houses was 9.1% versus 20.1% for those living in unscreened houses (OR = 0.44, 95% CI 0.27 - 0.71). The proportion of houses with closed eaves increased from 66.0% in 2009 to 74.3% in 2012 (test for trend p = 0.01). The proportion of screened houses remained unchanged over time at 1.3%.Conclusion/SignificanceAs a malaria control intervention, house modification has the advantages that it is not affected by the growing threat of insecticide resistance; it protects all household members equally and at all times while indoors; and it offers protection against a number of vector borne diseases. The study provides evidence in support of efforts to regulate or encourage housing improvements which impede vector access into residences as part of an integrated vector control approach to complement existing measures which have been only partially successful in reducing malaria transmission in some parts of Bioko.

The effect of screening doors and windows on indoor density of Anopheles arabiensis in south-west Ethiopia: a randomized trial

BackgroundScreening of houses might have impact on density of indoor host-seeking Anopheles mosquitoes. A randomized trial of screening windows and doors with metal mesh, and closing openings on eves and walls by mud was conducted to assess if reduce indoor densities of biting mosquitoes.MethodsMosquitoes were collected in forty houses using Centers for Diseases Control and Prevention (CDC) light traps biweekly in March and April 2011. A randomization of houses into control and intervention groups was done based on the baseline data. Windows and doors of 20 houses were screened by metal mesh, and openings on the walls and eves closed by mud and the rest 20 houses were used as control group. Mosquitoes were collected biweekly in October and November 2011 from both control and intervention houses. A Generalized Estimating Equations (GEE) with a negative binomial error distribution was used to account for over dispersion of Anopheles arabiensis and culicine counts and repeated catches made in the same house.ResultsScreening doors and windows, and closing openings on eves and wall by mud reduced the overall indoor densities of An. arabiensis by 40%. The effect of screenings pronounced on unfed An. arabiensis by resulting 42% reduction in houses with interventions. The total costs for screening windows and doors, and to close openings on the eves and walls by mud was 7.34 USD per house.ConclusionScreening houses reduced indoor density of An. arabiensis, and it was cheap and can easily incorporated into malaria vector strategies by local communities, but improving doors and windows fitness for screening should be considered during house construction to increase the efficacy of screenings.

Direct and Unbiased Information Recovery from Liquid Chromatography–Mass Spectrometry Raw Data for Phenotype-Differentiating Metabolites Based on Screening Window Coefficient of Ion Currents

A reworking of a data mining strategy, in which statistical treatment of raw data from liquid chromatography-mass spectrometry (LC-MS) precedes recognition of chromatographic peaks, is presented. In this algorithm the tR-m/z plane of LC-MS data is divided into equal-sized segments of twelve seconds by one m/z unit each, and the total ion currents in corresponding segments as specified by the tR-m/z pair from multiple LC-MS runs are evaluated to generate mean ion currents (μ) and standard deviations (σ). The μ's and σ's of the segments, derived from contrasting classes of LC-MS data set (e.g., resistant-susceptible, case-control, etc.), are used to calculate the Z-factor (screening window coefficient) which is in turn used to rank the segments. Chromatographic peaks are recognized only where the ion currents are shown to differentiate the classes. The result-reporting format enables detection of positive as well as negative correlations between ion intensities and biological traits under study and thus points to the presence of potentially phenotype-discriminating metabolites. Examples of data analyses are presented, in which ions that may distinguish resistant and susceptible species of Aesculus to the leaf-miner Cameraria ohridella were detected.

Impact of Community-Based Larviciding on the Prevalence of Malaria Infection in Dar es Salaam, Tanzania

BackgroundThe use of larval source management is not prioritized by contemporary malaria control programs in sub-Saharan Africa despite historical success. Larviciding, in particular, could be effective in urban areas where transmission is focal and accessibility to Anopheles breeding habitats is generally easier than in rural settings. The objective of this study is to assess the effectiveness of a community-based microbial larviciding intervention to reduce the prevalence of malaria infection in Dar es Salaam, United Republic of Tanzania.Methods and FindingsLarviciding was implemented in 3 out of 15 targeted wards of Dar es Salaam in 2006 after two years of baseline data collection. This intervention was subsequently scaled up to 9 wards a year later, and to all 15 targeted wards in 2008. Continuous randomized cluster sampling of malaria prevalence and socio-demographic characteristics was carried out during 6 survey rounds (2004–2008), which included both cross-sectional and longitudinal data (N = 64,537). Bayesian random effects logistic regression models were used to quantify the effect of the intervention on malaria prevalence at the individual level. Effect size estimates suggest a significant protective effect of the larviciding intervention. After adjustment for confounders, the odds of individuals living in areas treated with larviciding being infected with malaria were 21% lower (Odds Ratio = 0.79; 95% Credible Intervals: 0.66–0.93) than those who lived in areas not treated. The larviciding intervention was most effective during dry seasons and had synergistic effects with other protective measures such as use of insecticide-treated bed nets and house proofing (i.e., complete ceiling or window screens).ConclusionA large-scale community-based larviciding intervention significantly reduced the prevalence of malaria infection in urban Dar es Salaam.

Arboviral Surveillance of Field-Collected Mosquitoes Reveals Circulation of West Nile Virus Lineage 1 Strains in Eastern Thrace, Turkey

Arbovirus screening in invertebrate vectors is an important component of the vector-borne disease surveillance programs. Turkey has been shown to harbor medically important mosquito-borne arboviruses such as West Nile Virus (WNV). However, limited information about infections in vectors are currently available. This study was performed to provide preliminary data from Eastern Thrace region, Turkey, where no arbovirus vector surveillance has previously been performed. Mosquito sampling was undertaken at 23 sites in Edirne province during July, 2012. All specimens were identified morphologically, and selected individuals were subjected to DNA barcoding via cytochrome c oxidase I (COI) sequencing. Consensus PCR for Flavivirus, Alphavirus, and Phlebovirus genera and WNV-specific nested and real-time reverse transcription PCR were employed for mosquito pool screening and/or confirmation. Viral sequences detected in pools were characterized via sequencing. A total of 9261 mosquitoes were captured and distributed into 232 pools from the following species: Ochlerotatus caspius (90.9%), Culex pipiens sensu lato (s.l.) (4.7%), Anopheles pseudopictus (3%), and Anopheles maculipennis s.l. (1.3%). Specimens morphologically classified as Cx. pipiens s.l. were identified as Cx. pipiens pipiens via barcoding. Thirty-seven mosquito pools (15.9%) were positive in pan-flavivirus and WNV-specific assays. Viral sequences in positive pools were characterized as WNV lineage 1 clade 1a and demonstrated 1-4% divergence. No flavivirus sequences other than WNV were detected in the mosquito pools. WNV infection rates in Oc. caspius and Cx. pipiens s.l. pools were 15.6% and 36.3%, respectively. Comparison of current and previously identified WNV sequences from Turkey revealed 94.00-96.34% similarity. WNV RNA was identified for the first time in Cx. pipiens s.l. and Oc. caspius mosquitoes in Eastern Thrace, Turkey. Our findings indicate the circulation of WNV lineage 1 strains in potential vector species and provide an epidemiological link between WNV activity in mosquitoes and vertebrate infections.

Widespread purifying selection on RNA structure in mammals.

Evolutionarily conserved RNA secondary structures are a robust indicator of purifying selection and, consequently, molecular function. Evaluating their genome-wide occurrence through comparative genomics has consistently been plagued by high false-positive rates and divergent predictions. We present a novel benchmarking pipeline aimed at calibrating the precision of genome-wide scans for consensus RNA structure prediction. The benchmarking data obtained from two refined structure prediction algorithms, RNAz and SISSIz, were then analyzed to fine-tune the parameters of an optimized workflow for genomic sliding window screens. When applied to consistency-based multiple genome alignments of 35 mammals, our approach confidently identifies >4 million evolutionarily constrained RNA structures using a conservative sensitivity threshold that entails historically low false discovery rates for such analyses (5–22%). These predictions comprise 13.6% of the human genome, 88% of which fall outside any known sequence-constrained element, suggesting that a large proportion of the mammalian genome is functional. As an example, our findings identify both known and novel conserved RNA structure motifs in the long noncoding RNA MALAT1. This study provides an extensive set of functional transcriptomic annotations that will assist researchers in uncovering the precise mechanisms underlying the developmental ontologies of higher eukaryotes.

Dengue Knowledge and Preventive Practices among Rural Residents in Samar Province, Philippines

Background: The World Health Organization (WHO) classifies dengue as a disease important in public health. The epidemiology and ecology of dengue infections are strongly associated with human habits and activities. Aim: The present study aimed to evaluate the knowledge and practices regarding dengue infections among rural residents in Samar Province, Philippines. Methods: A cross sectional design was adopted for this investigation A convenience samples of six hundred forty six (646) residents who were visiting the rural health units in different municipalities of Samar, Philippines were taken as participants in study. Results: More than half of the respondents had good knowledge (61.45%) on causes, signs and symptoms, mode of transmission, and preventive measures about dengue. More than half of the respondents used dengue preventive measures such as fans (n = 340, 52.63%) , mosquito coil (n = 458, 70.90%), and bed nets (n = 387, 59.91%) to reduce mosquitoes while only about one third utilized insecticides sprays (n = 204, 31.58%) and screen windows (n = 233, 36.07%) and a little portion used professional pest control (n = 146, 22.60%). There was no correlation between knowledge about dengue and preventive practices (p=0.75). Television/Radio was cited as the main source of information on dengue infections. Conclusions: Findings suggest that better knowledge does not necessarily lead to better practice of dengue measures. Educational campaigns should give more emphasis on cost effective ways of reducing mosquito and preventing dengue such as environmental measures and control. Furthermore, wide range of information, skills and support must be provided by the government to increase dengue awareness among residents. Keywords: Dengue fever, dengue preventive practices, dengue knowledge, Samar Province DOI: http://dx.doi.org/10.11591/ijphs.v2i2.2511 Full Text: PDF

Dengue Knowledge and Preventive Practices among Rural Residents in Samar Province, Philippines

The World Health Organization (WHO) classifies dengue as a disease important in public health. The epidemiology and ecology of dengue infections are strongly associated with human habits and activities. The present study aimed to evaluate the knowledge and practices regarding dengue infections among rural residents in Samar Province, Philippines. A cross sectional design was adopted for this investigation. Convenience samples of six hundred forty six (646) residents who were visiting the rural health units in different municipalities of Samar, Philippines were taken as participants in study. More than half of the respondents had good knowledge (61.45%) on causes, signs and symptoms, mode of transmission, and preventive measures about dengue. More than half of the respondents used dengue preventive measures such as fans (n = 340, 52.63%), mosquito coil (n = 458, 70.90%), and bed nets (n = 387, 59.91%) to reduce mosquitoes while only about one third utilized insecticides sprays (n = 204, 31.58%) and screen windows (n = 233, 36.07%) and a little portion used professional pest control (n = 146, 22.60%). There was no correlation between knowledge about dengue and preventive practices (p=0.75). Television/Radio was cited as the main source of information on dengue infections. Findings suggest that better knowledge does not necessarily lead to better practice of dengue measures. Educational campaigns should give more emphasis dengue transmissions and on cost effective ways of reducing mosquito and preventing dengue such as environmental measures and control. Furthermore, wide range of information, skills and support must be provided by the government to increase dengue awareness among residents.

PP.47 Evaluating Outcomes of Skeletal Dysplasias (SDs)

ObjectiveTo evaluate the outcome of 543 pregnancies identified with a skeletal dysplasia or an antenatal suspected skeletal anomaly in the East Midlands and South Yorkshire (EMSYCAR) Congenital Anomaly Register over...

Postpartum alterations in circulating endothelial progenitor cells in women with a history of pre-eclampsia.

To characterize persistent postpartum maternal endothelial dysfunction following pre-eclampsia (PE) through the assessment of endothelial progenitor cells as markers of endothelial reparative capacity. Maternal circulating endothelial progenitor cells were measured at 2months and 6months postpartum in women who had recently experienced PE pregnancies (n=17). Normotensive controls (n=13) with uncomplicated pregnancies served for comparison at the same time points. Progenitor cells were measured by flow cytometry and by colony forming units. Maternal cardiovascular risk was measured at 6months postpartum. Levels of maternal circulating endothelial progenitor cells and cardiovascular risk in the early postpartum period of uncomplicated and PE pregnancies. CD34+VEGFR-2+ and CD133+VEGFR-2+ cells were elevated in PE subjects at 2months postpartum compared to healthy control subjects, although reduced by 6months postpartum. PE was associated with reduced colony forming units at 2 and 6months postpartum. Cardiovascular risk scores were increased in PE compared to normotensive controls. We have demonstrated that there is a physiological alteration in the number and function of circulating progenitor cells following PE pregnancies. Furthermore, this population of women exhibited elevated cardiovascular risk profiles compared to those with uncomplicated pregnancies. Pregnancy and the development of PE identify an early window for cardiovascular risk screening in women. Cellular markers of vascular health offer an approach to the investigation of postpartum endothelial dysfunction.

Measurements of tumor size using CT and PET compared to histopathological size in non-small cell lung cancer

In this study, we aimed to compare the tumor sizes determined by maximum morphological computed tomography (CT) and functional positron emission tomography (PET) with the histopathological size to determine which method provides the best correlation with the histopathological size in lung carcinoma patients. Forty lung carcinoma patients (39 males, one female) diagnosed histopathologically from surgical resection materials were included in this retrospective study. The mean age (±standard deviation, SD) of the patients was 67.8±10.3 years with a range of 44 to 81 years. The PET scans were performed within the same week as the CT scan. In the CT scans, the morphological tumor sizes were measured three-dimensionally by the longest transaxial section in the parenchymal and mediastinal screening window. The functional tumor sizes were also measured three-dimensionally in the PET scans. These two measurement values were compared with the histopathological size using Bland-Altman plotting. Bland-Altman plotting was also performed to define the 95% limits of agreement, which was presented as the bias ±1.96 SD. The histopathological sizes were measured in a range of 1.2 to 7.5 cm. The maximum measurement of the tumors on the CT scans showed a lower concordance (mean difference, -0.30) than that obtained from PET, and the SD was found to be larger than the PET (1.96 SD was 3.50 for CT and 2.50 for PET). The PET measurements of tumor size were more compatible with the histopathological sizes than the CT measurements in patients with non-small cell lung cancer.

A need for better housing to further reduce indoor malaria transmission in areas with high bed net coverage

BackgroundThe suppression of indoor malaria transmission requires additional interventions that complement the use of insecticide treated nets (ITNs) and indoor residual spraying (IRS). Previous studies have examined the impact of house structure on malaria transmission in areas of low transmission. This study was conducted in a high transmission setting and presents further evidence about the association between specific house characteristics and the abundance of endophilic malaria vectors.MethodsMosquitoes were sampled using CDC light traps from 72 randomly selected houses in two villages on a monthly basis from 2008 to 2011 in rural Southern Tanzania. Generalized linear models using Poisson distributions were used to analyze the association of house characteristics (eave gaps, wall types, roof types, number of windows, rooms and doors, window screens, house size), number of occupants and ITN usage with mean catches of malaria vectors (An.gambiae s.l. and An. funestus).ResultsA total of 36490 female An. gambiae s.l. were collected in Namwawala village and 21266 in Idete village. As for An. funestus females, 2268 were collected in Namwawala and 3398 in Idete. Individually, each house factor had a statistically significant impact (p < 0.05) on the mean catches for An. gambiae s.l. but not An. funestus. A multivariate analysis indicated that the combined absence or presence of eaves, treated or untreated bed-nets, the number of house occupants, house size, netting over windows, and roof type were significantly related (p < 0.05) to An.gambiae s.l. and An. funestus house entry in both villages.ConclusionsDespite significant reductions in vector density and malaria transmission caused by high coverage of ITNs, high numbers of host-seeking malaria vectors are still found indoors due to house designs that favour mosquito entry. In addition to ITNs and IRS, significant efforts should focus on improving house design to prevent mosquito entry and eliminate indoor malaria transmission.

Foodborne Disease Prevention and Broiler Chickens with ReducedCampylobacterInfection

Studies have suggested that flies play a linking role in the epidemiology of Campylobacter spp. in broiler chickens and that fly screens can reduce the prevalence of Campylobacter spp. We examined the year-round and long-term effects of fly screens in 10 broiler chicken houses (99 flocks) in Denmark. Prevalence of Campylobacter spp.–positive flocks was significantly reduced, from 41.4% during 2003–2005 (before fly screens) to 10.3% in 2006–2009 (with fly screens). In fly screen houses, Campylobacter spp. prevalence did not peak during the summer. Nationally, prevalence of Campylobacter spp.–positive flocks in Denmark could have been reduced by an estimated 77% during summer had fly screens been part of biosecurity practices. These results imply that fly screens might help reduce prevalence of campylobacteriosis among humans, which is closely linked to Campylobacter spp. prevalence among broiler chicken flocks.

Numerical investigation of particle deposition inside aero-shielded solar cyclone reactor: A promising solution for reactor clogging

Solar cracking of methane is considered to be an attractive option due to its CO2 free hydrogen production process. Carbon particle deposition on the reactor window, walls and exit is a major obstacle to achieve continuous operation of methane cracking solar reactors. As a solution to this problem a novel “aero-shielded solar cyclone reactor” was created. In this present study the prediction of particle deposition at various locations for the aero-shielded reactor is numerically investigated by a Lagrangian particle dispersion model. A detailed three dimensional computational fluid dynamic (CFD) analysis for carbon deposition at the reactor window, walls and exit is presented using a Discrete Phase Model (DPM). The flow field is based on a RNG k–ε model and species transport with methane as the main flow and argon/ hydrogen as window and wall screening fluid. Flow behavior and particle deposition have been observed with the variation of main flow rates from 10–20L/min and with carbon particle mass flow rate of 7×10−6 and 1.75×10−5kg/s. In this study the window and wall screening flow rates have been considered to be 1L/min and 10L/min by employing either argon or hydrogen. Also, to study the effect of particle size simulations have also been carried out (i) with a variation of particle diameter with a size distribution of 0.5–234μm and (ii) by taking 40μm mono sized particles which is the mean value for the considered size distribution. Results show that by appropriately selecting the above parameters, the concept of the aero-shielded reactor can be an attractive option to resolve the problem of carbon deposition at the window, walls and exit of the reactor.

Oxidative stress, photodamage and the role of screening pigments in insect eyes

Using red-eyed mutant triatomine bugs (Hemiptera: Reduvidae), we tested the hypothesis of an alternative function of insect screening pigments against oxidative stress. To test our hypothesis, we studied the morphological and physiological changes associated with the mutation. We found that wild-type eyes possess a great amount of brown and red screening pigment inside the primary and secondary pigment cells as well as in the retinular cells. Red-eyed mutants, however, have only scarce red granules inside the pigmentary cells. We then compared the visual sensitivity of red-eyed mutants and wild types by measuring the photonegative responses of insects reared in light:dark cycles [12 h:12 h light:dark (LD)] or constant darkness (DD). Finally, we analyzed both the impact of oxidative stress associated with blood ingestion and photodamage of UV light on the eye retina. We found that red-eyed mutants reared in DD conditions were the most sensitive to the light intensities tested. Retinae of LD-reared mutants were gradually damaged over the life cycle, while for DD-reared insects retinae were conserved intact. No retinal damage was observed in non-fed mutants exposed to UV light for 2 weeks, whereas insects fed on blood prior to UV exposure showed clear signs of retinal damage. Wild-type insects exposed to UV light showed a marked increase in the amount and density of screening pigments.

영상처리를 이용한 상업용 전자칠판의 인터페이스 구현

본 논문에서는 상업용 아임센서터치 전자칠판(Interactive Whiteboard System, IWB)을 소개한다. 이 시스템은 손가락이나 펜을 이용하여 접촉식 상호작용이 가능한 화이트보드 스크린을 통해 개인용 컴퓨터를 운용할 수 있도록 도와주는 인터페이스(Interface)이다. 제안된 인터페이스는 윈도우즈 운영체제와 상호작용하며, 온도와 조명의 변화에 적응적으로 동작한다. 제안된 시스템은 카메라에서 입력된 수광부(Optical Receptive Field)의 영상을 참조영상과 비교하여 차이를 계산하고, 그 차이를 이용하여 터치스크린의 좌표값을 계산한다. 계산된 좌표값을 기반으로 윈도우즈 마우스 이벤트를 생성하여 윈도우즈시스템으로 전달한다. 우리는 참조영상을 갱신하기 위해 두 개의 스레드(Thread)을 이용한 임계영역을 구현하고, 차이계산의 신뢰성을 위해 적응적 임계값을 이용한 참조영상의 갱신을 구현한다. 제안된 터치스크린 인터페이스를 장착한 전자칠판 시스템은 향후 국내외 시장의 성장률이 높아 전도유망한 상품이며, 시장성이 밝을 것으로 기대한다. In this paper we introduce a commercial interactive whiteboard (IWB) system named ImSensorTouch by ImSensor Inc. Using this interface system, we can control our computer through the interactive whiteboard screen just by touching it with your finger or a pen. The interface interacts with Windows operating system (OS) and is adaptable to changes of surroundings especially temperature, and illumination condition. The proposed system calculates the difference between a reference image and a current image captured by a camera in the optical receptive field. And the position making the difference is used to generate the position on Windows screen. Then, we send a mouse event on the position to Windows OS. We have implemented the system using a critical section(CS) with two threads for the reference frame update process in which an adaptive thresholding technique is periodically exploited to get reliable result. We expect the system is competitive and promises a bright future in the IWB market.

Cell polarity: models and mechanisms from yeast, worms and flies

Determinants of cell polarity orient the behaviour of many cell types during development. Pioneering genetic screens in yeast, worms and flies have identified key polarity determinants that are evolutionarily conserved across the animal kingdom. Recent work in these three model organisms has combined computer modelling with experimental analysis to reveal the molecular mechanisms that drive the polarisation of determinants. Two key principles have emerged: the first is the requirement for a positive-feedback loop to drive self-recruitment of determinants to the plasma membrane; the second is the requirement for mutual antagonism between determinants that localise to opposite ends of the cell.

Nosocomial myiasis in a patient with diabetes

This report describes a case of nosocomial myiasis caused by larvae of Sarcophaga (Bercaea) africa. The parasitosis developed in an ulcer on the heel of a patient with type 2 diabetes. The ulcer was dressed when the deposition occurred. An experiment was performed in order to demonstrate the ability of Sarcophaga larvae to move through bandages and reach purulent wounds. This report highlights the need for particular attention, education and specific protocols in hospitals in order to avoid myiasis, which can compromise the organization's reputation for hygienic standards with possible legal consequences.