The aerodynamic characteristics of twin-separated parallel decks for long-span rail-cum-road bridges with various wind barriers clamped at railway girder are more complicated because of aerodynamic interference. Experimental investigations on aerodynamic coefficient and wind pressure coefficients of twin girders under different incoming flow directions were conducted using wind tunnel tests. Three different heights ( H) varying from 2 m to 4 m and four different ventilation ratios ( R) varying from 20% to 50% for wind barriers were examined. Results indicated that the aerodynamic coefficients were affected significantly by the incoming wind direction, which must be considered, and the railway wind barrier affected the aerodynamic characteristics of the railway girder when it is located both upstream and downstream, but the laws are not the same. Due to the aerodynamic interference effect of the twin parallel girders, the aerodynamic characteristics of the adjacent highway girders were also affected by the wind barrier along the railway girder, especially when the highway girders were located downstream. While, the highway girders were less affected when located upstream. The influence of wind barrier height and ventilation ratios on the adjacent highway girders was completely different. Most importantly, the parameters optimization of wind barriers clamped at railway girders should be determined after a comprehensive evaluation of the aerodynamic characteristic of both the railway and highway girders.