The AASHTO 2001 Standard Specifications for Structural Supports for Highway Signs, Luminaires and Traffic Signals include revised wind load provisions and new criteria for fatigue design. These provisions and criteria differ considerably from those in previous editions of the specifications, and their impact on the design of cantilevered overhead sign supports has not been fully studied. This study assesses the effect of these provisions and criteria on the design of cantilevered overhead sign support structures with the horizontal support composed of a four-chord truss. Wind and fatigue load design calculations of typical structures, located at sites across the United States, were performed with the design provisions of the 2001 supports specifications and compared with design in accordance with the previous edition of the specifications. The induced forces in the primary members of the cantilevered sign support structure were calculated, and corresponding member sizes and weights were estimated. The results of the study demonstrated the effect of the wind and fatigue load provisions on the design of cantilevered overhead sign support structures.