Aim: To evaluate the antibiotic susceptibility of bacteria isolated from the pyogenic abscess of dental origin. Objective: To assess the bacterial isolates of the pyogenic abscesses of dental origin and evaluate the antibiotic susceptibility of the same. Study Design: The study was an in-vitro experimental open clinical trial. Surgical aspiration of pus was done in 30 patients suffering from pyogenic abscesses of dental origin. The pus sample was subjected to standard methods for isolation and identification of microorganisms as per Topley and Wilson method and antimicrobial susceptibility by the method of Kirby‑Bauer was done. Results: Aerobes were isolated in 22 instances (70%), of which facultative anaerobes were isolated in 4 instances. Pure isolates of anaerobes were isolated in 4 instances (13.3%). In four cases no organisms were isolated. In this study Staphylococcus aureus was isolated in 13 instances (43.3%), Streptococcus viridians in 4 instances (13.3%) followed by anaerobic Streptococci in 4 instances (13.3%). The susceptibility and resistance of bacteria to 12 antibiotic panels most recommended for Gram positive organism showed 100% susceptibility for Ceftriaxone, Amoxyclav and Amikacin, while 95% for Cefuroxime and Sparfloxacin. Maximum resistance was noted with Amoxycilin in 7 cases (37%) followed by azilides group. The Gram negative isolates showed 100% sensitivity for the 9/10 antibiotic panel. 2 cases were resistant to Ampicillin. Anaerobic isolates tested against panel of 10 drugs showed 100% susceptibility to all. Conclusion: Within the limits of the present study, it can be concluded that pyogenic abscesses of dental origin has isolates of mixed nature. The study results can act as a guide for the clinician while prescribing antibiotics. Access this article online Website: www.iadrsd.org Quick response code