The Wassenaar Wildlife Breeding Centre was formerly a part of Wassenaar Zoo but has been an independent institution since 1984. The Centre, which is not open to the public, has established a breeding protocol for Cheetah Acinonyx jubatus which has resulted in 58.54 births since 1980. This paper describes the husbandry and breeding of Cheetahs and the development of cubs. Behavioural comparisons on Cheetahs at Wassenaar and five other institutions, and observations on the behaviour of two ♀♀' and the breeding behaviour of one ♂ are also described.