Wild Quail Research Articles

Experiences in Northern Bobwhite Propagation and Translocation in Ohio, 1978-2012

Ohio once boasted a population of 7 million northern bobwhites (Colinus virginianus). Catastrophic blizzards during 1976–1977 and 1977–1978 brought winter quail mortalities of 85% and 80%, respectively. Ohio’s bobwhite population was 430,000 in 1978, a 90% reduction from 1976. Remnant quail populations were small, isolated, and incapable of rapid recovery. The Ohio Department of Natural Resources (ODNR) initiated a statewide stocking effort to expedite population growth. The ODNR chose to propagate wild quail in captivity and release first-generation progeny (F1). Throughout 1980–1986, the ODNR released 65,000 F1 quail statewide. The ODNR monitored population response with North American Breeding Bird Survey, mail carriers, and roadside whistle-counts, but found little evidence of success. The ODNR evaluated postrelease survival and productivity of F1 hens (n = 100) and wild hens (n = 40) during 1984–1986 and deemed F1 hens inferior to wild hens. In 1993, the ODNR produced population models to develop minimum stocking rates for wild quail translocation, but insufficient numbers were captured. From 1998 to 2000 and 2005 to 2009, the ODNR translocated wild quail from Kansas to 5 Ohio sites with suitable, unoccupied habitat. Concurrently the ODNR translocated wild quail within Ohio. The ODNR conducted annual spring whistle-counts on all release sites during 1998–2012. Little or no evidence of sustained populations existed on sites after 7 years. Bobwhite translocation may yet show promise for population restoration, but evaluation should include 7–10 years of monitoring at a minimum.

Development and Implementation of a Successful Northern Bobwhite Translocation Program in Georgia

Gamebird translocations have been used for many years to establish or re-establish populations in North America. A long-term severe decline of northern bobwhites (Colinus virginianus) over much of their range has brought this issue to the forefront for this species as well. Field studies in Georgia over the last decade have documented site fidelity, high survival, reproductive success, and population response from bobwhites translocated into large blocks of well-managed habitat. The Georgia Department of Natural Resources/ Wildlife Resources Division developed an official wild quail translocation policy in 2006. Five translocation projects have been permitted and conducted since the policy was established. Extensive large scale habitat modifications were required on the recipient sites while donor sites were required to have existing high density bobwhite populations. These projects have resulted in translocation of . 800 bobwhites and the establishment of 7,480 ha of new wild quail population centers thereby contributing to the National Bobwhite Conservation Initiative (NBCI) recovery goals. Average fall densities on completed projects have increased from , 0.5 birds/ha to . 1.25 birds/ha.

Free-ranging, Northern Bobwhite Submissions to the Southeastern Cooperative Wildlife Disease Study (1982-2015)

There are concerns regarding population declines of northern bobwhite (Colinus virginianus) over the past 4 decades (Palmer et al. 2011). Infectious and noninfectious diseases are among the limiting factors that potentially influence bobwhite demographics (Applegate 2014). The last update of diseases of bobwhite was presented at the Second National Quail Symposium in 1982 (Davidson et al. 1982). Since that report, scientists at the Southeastern Cooperative Wildlife Disease Study (SCWDS) have examined 133 wild bobwhites from 13 states. The SCWDS is a cooperative between states and the University of Georgia and obtains cases from the cooperating states. In this update, we focus on the diagnostic testing results from wild birds and exclude other cases that were examined during this period. We searched the SCWDS database for all bobwhite cases 1985–2016 and examined the individual case reports for 133 wild bobwhite quail. During this period, the majority of cases originated from Florida, Georgia, and Kansas, where research was being conducted on bobwhite populations. A diagnosis could not be clearly identified in all cases and some otherwise healthy bobwhites were submitted for screening; therefore, we have narrowed the focus of this report to a subset of 78 bobwhites. Wild bobwhites that were submitted by SCWDS state cooperators had an approximately even distribution between male and female birds (26 F: 19 M; 2 unknown sex). Adults (20 F, 10 M) predominated over juvenile birds (6 F, 7 M, 2 unknown sex). Trauma (physical injury) was the diagnosis in 17 female and 38 male bobwhites submitted during this period. Three each of male and female birds were considered to have no health problems. Some of the most frequent findings in diagnosed bobwhites were possible Physaloptera sp. infection (n = 9, 17.0%), avian pox (n = 7, 14.9%), intoxication (lead and carbamate; n = 5, 10.6%), corneal opacity (n = 4, 8.5%), Sarcocystis sp. infection (n = 3, 6.4%), and fungal pneumonia (n = 2, 4.25%). Some parasitic infections (e.g., coccidiosis) were thought to be associated with mortality based on necropsy and laboratory findings while a number of the parasites were determined to be incidental findings (e.g., Sarcocystis and Physaloptera) based on necropsy and laboratory findings. Corneal opacity was found in 4 birds, but the cause was not determined. The most striking findings were that trauma (e.g., physical injury) or avian pox were among the most common causes of mortality in free-ranging quail. Iatrogenic (researcher) causes of mortality (n = 5, 10.6%) associated with complications from radiotransmitters and small mammal trapping also occurred. This latter urges careful consideration among bobwhite researchers. The cause of population declines in bobwhites are likely multifactorial. We hope that morbidity and mortality investigations can provide some insight into potential limiting factors for bobwhites and assist wildlife managers with population management decisions.

Exposure of Captive Bobwhites to an At-Planting Application of Terbufos (Counter 15G) to Corn

Terbufos is a highly toxic, organophosphate insecticide that is commonly applied to com fields during planting. Quail use crop field edges during April, when com is planted in North Carolina, and consequently may be exposed to at-planting insecticides. Therefore, we attempted to quantify the hazards to quail from an at-planting, banded application of Counter® 15G using penned northern bobwhite quail. Eight, 7.5 X 7.5m pens were placed in cornfields immediately after planting. Six field pens received Counter 15G at 22.7g per IOOm of cornrow. Pens were placed such that a 2.5 X 7.5m section was located in standing wheat. The remainder of each pen extended past the "turnrows" into a section of regular rows in each cornfield. Two quail of each sex were placed in each pen. Behavior of quail using cornfields was observed from blinds and categorized as feeding, loafing, dusting and other. Blood serum, for determining cholinesterase (ChE) activity, was collected from a sub-sample of quail (n = 3) from each pen prior to and at 1.5, 8.5 and 15.5 days following exposure. Change in (ChE) activity from pre-exposure levels was determined and averages for birds from each pen were compared between treatments using a one-way analysis of variance. In quail exposed to terbufos, serum ChE activity declined 21% relative to pre-exposure levels at 1.5 days (P = 0.04; df = 1,4), but not at later dates sampled (P > 0.08). No mortality was observed. Observations of quail in pens revealed no unusual behaviors or changes in behavioral patterns over the course of the study. Our results suggest that Counter® 15G is unlikely to cause mortality or significant behavioral changes in wild quail inhabiting farms.

Executive Summary, National Bobwhite Conservation Initiative, March 2011, NBCI 2.0...The Unified Strategy to Restore Wild Quail

Executive Summary, National Bobwhite Conservation Initiative, March 2011, NBCI 2.0...The Unified Strategy to Restore Wild Quail

Exclosures: An Experimental Technique for Protection of Northern Bobwhite Nests

Nest predation has been implicated as a factor affecting northern bobwhite (Colinus virginianus) recruitment rates. Public stakeholders are increasingly questioning use of lethal methods to manage predation. We evaluated a nonlethal method consisting of single nest treatments using an exclosure to protect nests from potential predators. The exclosure treatment also included use of Amdrot (hydramethylnon) and Snake-a-wayt repellents to deter red-imported fire ants (Solenopsis invicta) and snakes, respectively. We compared nest success of treated (n 1⁄4 8) to untreated nests (n 1⁄4 18). Treated nests were 88% successful which was a 2-fold increase over unprotected nests. We did not observe any difference in hen behavior between treatment and controls. This technique may be useful to study nest success of wild quail and is not intended to be a management technique to influence overall population growth.

Impact on Base Population Density and Hunter Performance of Stocking with Pen-Raised Bobwhite

In 1962, the Kansas Fish and Game Commission initiated an investigation to determine the effect of semiannual releases of pen-raised bobwhite quail (Colinus virginianus) on population densities of native wild quail populations, on availability of birds to hunters, and on hunter success.Stocking during spring resulted in 7% fewer birds in the fall population on the stocked area than on the control area. Stocking during fall resulted in 14% more birds, at the time hunting season began, on the stocked area than on the control area. Neither of these differences were statistically significant, and it is concluded that there was no significant difference attributable to stocking between population densities of stocked and control areas. On the stocked area, however, there was a significant net increase of 25% in population density between the fall census period and the preseason census period. It is concluded that the density-depressing influence of spring stocking combined with the density-elevating influence of fall stocking, on the stocked area, produced a significant increase, attributable to stocking, between the population density preceding fall release and the population density preceding the hunting season. It is further concluded that in the comparison of preseason population densities for the treatment and control areas, the depressing effect of spring stocking and the elevating effect of fall stocking resulted in a treatment-area population that was significantly larger than that found on the unstacked control area.Some pen-raised birds established themselves as a part of the population on the stocked area, but there was not a proportional increase in population density. There were fewer native quail on the area when treated with semiannual stocking than when under control condition. The difference in density of native birds between stocked and control areas was not statistically significant. Stocking significantly increased hunter success by 30% and 35% on areas in Cherokee and Linn Counties, respectively, but the number of coveys flushed per hour was not significantly increased by stocking pen-raised quail.

Evaluating Hunting Success of Pen-Reared and Wild Northern Bobwhite in a Reclaimed Kentucky Mineland

Northern bobwhites (Colinus virginianus) have experienced severe population declines across their distribution. In order to address population declines and to continue providing hunting opportunities, multi-state efforts have been undertaken to stabilize and restore bobwhite populations. Ongoing efforts using the National Bobwhite Conservation Initiative’s quail focus area approach have so far demonstrated success throughout Kentucky. However, population increases in the Peabody Bobwhite Focal Area, in western Kentucky, have not been correlated to increases in perceived hunter success. Consequently, some sportsmen question the effectiveness of focal area conservation. In response to hunter concerns, we tested dog hunting ability with wild and pen-reared bobwhites. We also measured evasive behaviors of wild bobwhite using radiotelemetry. During the 2013–2014 and 2014–2015 hunting seasons we conducted 114 dog trials. Dogs detected bobwhite during 46 of 59 (78.0%) pen-reared trials and 16 of 55 (29.1%) wild bird trials. When dogs did not detect wild quail, birds ran away 64.1% of the time and remained motionless 20.5% of the time. Using an information-theoretic approach, we determined that bird type (wild vs. pen-reared) had a significant effect on bird detection, with dogs 8.62 times more likely to detect pen-reared birds than wild birds. We recommend that hunters be informed about differences in dog detection rates between pen-reared and wild bobwhite so that public support needed for wild bobwhite restoration can persist.

Population Response of Three Quail Species to Habitat Restoration in South Texas

Maintaining and increasing usable space is paramount for maintaining and increasing wild quail. Aside from weather and other factors that can temporarily reduce densities, range-wide, no factor has as much influence on quail populations as the amount of habitat present across the landscape. In the field of quail management, ‘‘bad news’’ is the norm, as many articles begin by explaining how a select species has declined. Here we provide good news and use 4 empirical examples of population increases for 3 quail species following creation of usable space and restoration of patch connectivity. From 2008–2014, a suite of independent projects aimed at increasing usable space for quail was initiated across South Texas. These projects included 3 focused on northern bobwhites (Colinus virginianus), 1 focused on scaled quail (Callipepla squamata), and 1 landowner-executed project focused on Montezuma quail (Cyrtonyx montezumae). Through the correction of attributes limiting habitat, bobwhite numbers increased 22–378% across 2 studies. On one particular study site, native grassland restoration resulted in the colonization of bobwhites from adjacent areas to 1 bobwhite/1.2 ha from nearly 0. For scaled quail in South Texas, reducing buffelgrass standing crop via grazing from about 2,240 kg/ha to 1,008 kg/ha resulted in the recolonization of a previously unoccupied habitat patch to a density of 1 scaled quail/6 ha. Finally, clearing monotypic stands of the invasive native plant, ash juniper (Juniperus ashei) in the Edwards Plateau of Texas, resulted in the reestablishment of native grasses and forbs and thus recolonization by Montezuma quail from nearby areas. Although habitat restoration and management can be a painstaking and lengthy process, addressing limiting factors to quail occupancy is the only known way to increase wild quail populations. We hope that highlighting these particular studies will provide inspiration to those interested in restoring and increasing quail across the US.

Nutritional Value of Farmed and Wild Quail Meats

Nutritional Value of Farmed and Wild Quail Meats

Amino Acid Levels in Muscle Tissue of Six Wild Feathered Species

The objective of this study was to determine and compare the levels of amino acids (AAs) in breast and thigh muscles of six species of feathered game of the same age. The experiment involved the following species: wild turkey (Meleagris gallopavo), guinea fowl (Numida meleagris), chukar partridge (Alectoris chucar), Japanese quail (Coturnix coturnix japonica), common pheasant (Phasianus colchicus) and grey partridge (Perdix perdix). The highest content of AAs was found in the chukar partridge (breast: 815.7 ± 47.71 g/kg; thigh: 771.4 ± 107.0 g/kg, on a dry matter basis), the lowest levels of AAs were found in Japanese quail (breast: 734.2 ± 45.07 g/kg) and grey partridge (thigh: 614.9 ± 49.66 g/kg). In all examined species, the level of histidine in breast muscles differed (P ≤ 0.01) from that in thigh muscles. In all investigated species, the levels of essential AAs in breast muscles were higher (P ≤ 0.01) than those in thigh muscles, whereas the levels of non-essential AAs in breast muscles were lower (P ≤ 0.01) than those in thigh muscles. Breast muscles are therefore more valuable than thigh muscles because of the content of essential AAs.

Eyeworm infections of Oxyspirura petrowi, Skrjabin, 1929 (Spirurida: Thelaziidae), in species of quail from Texas, New Mexico and Arizona, USA.

Northern bobwhite (Colinus virginianus) and Scaled quail (Callipepla squamata) have been declining steadily throughout much of their historical range over the past few decades. Even the Rolling Plains of Texas, historically rich with wild quail and one of the last remaining quail strongholds, has been suffering a population decline, most notably since 2010. Gambel's quail (Callipepla gambelii) have also been experiencing their own decline throughout their respective range, but not as significant as that of other species of quail. Eyeworms (Oxyspirura petrowi) in quail have been recognized for years but not thoroughly studied until recently. New research reveals that O. petrowi infection can cause inflammation, oedema, and cellular damage to the eye of the quail host. The objective of this research was to better understand the prevalence of the eyeworm infection in different quail species, expand on known distribution, and determine if there is a relationship between location and species infected with eyeworms. Northern bobwhite, Scaled quail and Gambel's quail were hunter-donated from one county within Texas, New Mexico and Arizona, and examined for the prevalence, mean abundance and mean intensity of eyeworm infection from November 2013 to February 2014. Quail from every location were found to have individuals with a varying degree of eyeworm infection. This is the first study to document eyeworm infection in Gambel's quail and in quail in New Mexico and Arizona, and reports the highest eyeworm infection found in Northern bobwhite and Scaled quail.

The role of nest‐site selection and cereal production in differential nest predation in Common Quail Coturnix coturnix and hybrid quail C. coturnix × C. japonica

In Europe, farm‐reared quail used for restocking purposes are often hybrids between the Common Quail Coturnix coturnix and the Japanese Quail Coturnix japonica. These hybrids interbreed with wild Common Quail populations and suffer higher rates of nest predation, which would suggest that the two quail types have different nest‐site selection patterns. We monitored 103 radiotagged nesting females (77 wild Common Quail over 18 breeding seasons and 26 hybrid quail over six breeding seasons) and analysed their nest placement behaviour. Our results did not provide any evidence that the quail types showed different nest‐site selection trends, as they all preferred to nest near cereal field margins. The higher nest predation rate experienced by hybrids might therefore be explained by less effective anti‐predator behaviour among hybrid females during incubation. As no edge effect on nest predation was observed, the preference for nesting close to cereal margins could be due to the greater food resources of field margins. Moreover, Common Quail nest predation decreased with a rise in barley straw production, supporting evidence that nest cover plays an important role in the prevention of nest predation in this species. No such relationship was found for hybrid quail, but this result is not conclusive, as the data on barley straw production for hybrid quail covered a much smaller range of interannual variability. Barley straw production in Catalonia decreased significantly between 1992 and 2012, possibly as a result of a change in the barley varieties used. This practice could entail a conservation threat for ground‐nesting bird species.

Multiyear Survey of Coccidia, Cryptosporidia, Microsporidia, Histomona, and Hematozoa in Wild Quail in the Rolling Plains Ecoregion of Texas and Oklahoma, USA.

We developed nested PCR protocols and performed a multiyear survey on the prevalence of several protozoan parasites in wild northern bobwhite (Colinus virginianus) and scaled quail (Callipepla squamata) in the Rolling Plains ecoregion of Texas and Oklahoma (i.e. fecal pellets, bird intestines and blood smears collected between 2010 and 2013). Coccidia, cryptosporidia, and microsporidia were detected in 46.2%, 11.7%, and 44.0% of the samples (n=687), whereas histomona and hematozoa were undetected. Coccidia consisted of one major and two minor Eimeria species. Cryptosporidia were represented by a major unknown Cryptosporidium species and Cryptosporidium baileyi. Detected microsporidia species were highly diverse, in which only 11% were native avian parasites including Encephalitozoon hellem and Encephalitozoon cuniculi, whereas 33% were closely related to species from insects (e.g. Antonospora, Liebermannia, and Sporanauta). This survey suggests that coccidia infections are a significant risk factor in the health of wild quail while cryptosporidia and microsporidia may be much less significant than coccidiosis. In addition, the presence of E.hellem and E.cuniculi (known to cause opportunistic infections in humans) suggests that wild quail could serve as a reservoir for human microsporidian pathogens, and individuals with compromised or weakened immunity should probably take precautions while directly handling wild quail.

Postcopulatory sexual selection favors fertilization success of restocking hybrid quails over native Common quails (Coturnix coturnix)

Postcopulatory sexual selection plays an important role in the reproductive success of males in many species. Differences in fertilization success could affect rates of admixture and genetic introgression between divergent lineages. We investigated sperm precedence in matings in captivity involving Common quails (Coturnix coturnix) and farm quails of hybrid origin (C. coturnix × domestic Japanese quail, C. japonica), the last used in restocking practices to increase hunting bags. These inter-specific matings in natural conditions are claimed to represent an important threat to the conservation of native Common quail populations. Results showed that fertilization success of each male depended on (1) the time it spent with the female, (2) the presence of sperm from a previous male in the female oviduct, (3) the time that the previous partner had been copulating with the female, and, most importantly, (4) the genetic origin of the male (wild or farm). Farm hybrid males showed higher fertilization success than wild Common males, and they required less time with the female to fertilize the same proportion of eggs. The presence of sperm from another male in the female oviduct reduced the percentage of fertilized eggs by a male. However, this reduction was higher for wild males when the precedent mate was a farm male. In summary, the sperm of farm hybrid males may outcompete the sperm of native males and this could be favoring the introgression of domestic Japanese alleles into the Common quail population, thus constituting a severe conservation threat to wild Common quail populations.

Cultivable bacterial microbiota of northern bobwhite (Colinus virginianus): a new reservoir of antimicrobial resistance?

The northern bobwhite (Colinus virginianus) is an ecologically and economically important avian species. At the present time, little is known about the microbial communities associated with these birds. As the first step to create a quail microbiology knowledge base, the current study conducted an inventory of cultivable quail tracheal, crop, cecal, and cloacal microbiota and associated antimicrobial resistance using a combined bacteriology and DNA sequencing approach. A total of 414 morphologically unique bacterial colonies were selected from nonselective aerobic and anaerobic cultures, as well as selective and enrichment cultures. Analysis of the first 500-bp 16S rRNA gene sequences in conjunction with biochemical identifications revealed 190 non-redundant species-level taxonomic units, representing 160 known bacterial species and 30 novel species. The bacterial species were classified into 4 phyla, 14 orders, 37 families, and 59 or more genera. Firmicutes was the most commonly encountered phylum (57%) followed by Actinobacteria (24%), Proteobacteria (17%) and Bacteroidetes (0.02%). Extensive diversity in the species composition of quail microbiota was observed among individual birds and anatomical locations. Quail microbiota harbored several opportunistic pathogens, such as E. coli and Ps. aeruginosa, as well as human commensal organisms, including Neisseria species. Phenotypic characterization of selected bacterial species demonstrated a high prevalence of resistance to the following classes of antimicrobials: phenicol, macrolide, lincosamide, quinolone, and sulphate. Data from the current investigation warrant further investigation on the source, transmission, pathology, and control of antimicrobial resistance in wild quail populations.

Decreased fitness of restocked hybrid quails prevents fast admixture with wild European quails

Restocking with non-native species for hunting purposes is a widespread practice in some Galliformes species that may result in the introgression of maladaptive alleles into native populations. Quails farmed for restocking are produced by interbreeding domestic Japanese quails (Coturnix japonica) with European quails (Coturnix coturnix). Massive releases of these animals could represent a threat to native European quails. In this study we radio-track 16 female native European quails and 51 female farm-reared quails over four breeding seasons in a single locality. Our results show that farmed female quails attracted more wild common quail males than European quail females, probably because they produce more rally calls. Here for the first time we show empirical evidence that European quails and restocked farmed quails interbreed in the wild. Further, hybrid farmed females and European females had similar probabilities of mating, nesting success, clutch size, fertility, hatching probability and chicks’ survival probability. However, female farmed quail had higher mortality than European quail females, and their nests had a higher rate of predation. These last observations could explain why the two lineages do not appear completely admixed after more than 20years of massive restocking practices. However, our results clearly show a lack of reproductive barriers in the wild and that introgression may not be completely prevented. An immediate ban of the release of non-native quails is necessary to preserve their genetic integrity. Thus, banning restocking with Japanese quail or hybrids is necessary to prevent the progressive introduction of maladaptive alleles into the European quail populations.

Detection of newcastle disease virus from wild quail (Coturnix coturnix) by reverse transcription polymerase chain reaction in Isfahan and Loretan Provinces of Iran

Detection of newcastle disease virus from wild quail (Coturnix coturnix) by reverse transcription polymerase chain reaction in Isfahan and Loretan Provinces of Iran

Hibridación entre la codorniz común (Coturnix coturnix) y la codorniz de granja: estado de un problema de conservación

Puigcerver, M., Sánchez-Donoso, I., Vilà, C., Sardà-Palomera, F., Morales-Rodríguez, P.A., Caballero de la Calle, J.R., Rodríguez-Teijeiro, J.D. (2013). Hybridization between the common quail (Coturnix coturnix) and farm-reared quails: state of the art of a conservation problem. Ecosistemas 22(2):48-53. Doi.: 10.7818/ECOS.2013.22-2.08 Hybridization between the common quail (Coturnix coturnix) and farm-reared quails: state of the art of a conservation problem. Hybridization between species is a widespread phenomenon which may have consequences in the conservation of the biodiversity. In this article we review the conservation problem derived from restocking practices with farm-reared quails in wild common quail populations (Coturnix coturnix). Farm quails have resulted to be hybrids of common quail and Japanese quail (Coturnix japonica). If there are not reproductive isolating mechanisms, restocking practices could favour the introgression of Japanese quail genes into common quail populations, thus resulting in a hybrid swarm and increased sedentariness of quail populations which would probably result in a population decrease in Europe. This is a real threat. There are neither pre-zigotic, post-zigotic, nor ecological barriers. However, empirical data does not suggest that a hybrid swarm has formed. Different rates of mortality between the species are a possible explanation. Lastly, we suggest some management actions given the current situation. Among them, we suggest genetic certification of the origin of farm-reared individuals and banning the restocking with Japanese quail or hybrids.

Preliminary Disease Surveillance in West Texas Quail (Galliformes: Odontophoridae) Populations

Northern Bobwhite (Colinus virginianus) and Scaled Quail (Callipepla squamata) occur throughout northwestern Texas and overall population numbers have been declining for the past 30 yr. This decline has been attributed to habitat loss associated with intensive agricultural practices. We propose that disease may be a contributing factor to decline. Our findings suggest that West Nile virus (WNV) infection may be common in wild quail populations on the Rolling Plains of northwestern Texas. Serum samples (n=301) from wild-caught Northern Bobwhite and Scaled Quail were collected during 2008-10 from seven private properties across the Rolling Plains Region; 5.3% had detectable antibodies against WNV using an enzyme-linked immunosorbent assay. To our knowledge, this is the first report of antibodies to WNV in Scaled Quail and wild-caught Northern Bobwhite from the Rolling Plains of Texas.