Brassicaceae Research Articles

Weed Control With Preemergence Herbicides in Azuki Bean

Three field experiments were completed over a three-year period (2019 to 2021) in Ontario, Canada to develop weed management programs in azuki bean with herbicides (pendimethalin, S-metolachlor, halosulfuron, and imazethapyr) applied alone and in combination, and metribuzin, applied preemergence (PRE). At 2 and 4 weeks after emergence (WAE), there was ≤ 8% azuki bean injury from the herbicide treatments evaluated, with the exception of the treatments that included S-metolachlor which caused up to 19% azuki bean injury. Pendimethalin (1080 g ai ha-1) and S-metolachlor (1600 g ai ha-1) controlled green foxtail 83-94% but provided poor control of common lambsquarters, wild mustard, redroot pigweed, common ragweed, and flower-of-an-hour. Imazethapyr (75 g ai ha-1) controlled common lambsquarters, wild mustard, redroot pigweed, and flower-of-an-hour 90-100% but provided 76-82% control of common ragweed and green foxtail. Halosulfuron (35 g ai ha-1) controlled wild mustard 100%, common ragweed 81-84%, common lambsquarters 77-83%, flower-of-an-hour 72-75%, redroot pigweed 59-72%, and green foxtail 19-23%. The tankmix of pendimethalin + S-metolachlor controlled green foxtail and common lambsquarters 87-97% but the control was only 23- 83% on wild mustard, redroot pigweed, common ragweed, and flower-of-an-hour. The tankmixes of pendimethalin + imazethapyr and pendimethalin + S-metolachlor + imazethapyr provided 90-100% control of common lambsquarters, wild mustard, redroot pigweed, flower-of-an-hour, and green foxtail, and 78-87% control of common ragweed. The tankmixes of pendimethalin + halosulfuron and pendimethalin + S-metolachlor + halosulfuron controlled common lambsquarters and wild mustard 91-100%, green foxtail 76-95%, flower-of-an-hour 70-94%, redroot pigweed 68-91%, and common ragweed 78-79%. Metribuzin (280 g ai ha-1) controlled common lambsquarters, wild mustard, redroot pigweed, common ragweed, flower-of-an-hour, and green foxtail up to 94, 98, 81, 58, 98, and 61% respectively; control improved to 99, 100, 97, 84, 99, and 83%, respectively when the rate was increased to 560 g ai ha-1. Generally, weed density and dry biomass reflected the level of weed control. Weed interference reduced azuki bean yield by 91% in this study. Generally, azuki bean yield reflected the level of weed control.

Interaction between 4-hydroxyphenylpyruvate dioxygenase–inhibiting and reactive oxygen species–generating herbicides for the control of annual weed species in corn

AbstractThe complementary modes of action of 4-hydroxyphenylpyruvate dioxygenase (HPPD) and photosystem II (PSII) inhibitors have been credited for the synergistic weed control improvement of several species. Recent research discovered that reactive oxygen species (ROS) generation and subsequent lipid peroxidation is the cause of cell death by the glutamine synthetase inhibitor glufosinate. Therefore, a basis for synergy exists between glufosinate and HPPD inhibitors, but the interaction has not been well reported. Four field experiments were conducted in Ontario, Canada, in 2020 and 2021 to determine the interaction between HPPD-inhibiting (mesotrione and tolpyralate) and ROS-generating (atrazine, bromoxynil, bentazon, and glufosinate) herbicides on control of annual weed species in corn (Zea mays L.). The ROS generators were synergistic with the HPPD inhibitors and provided ≥95% control of velvetleaf (Abutilon theophrasti Medik.), except for tolpyralate + glufosinate, which was additive at 8 wk after application (WAA) and provided 87% control. Tank mixes of HPPD inhibitors plus ROS generators were synergistic for the control of common ragweed (Ambrosia artemisiifolia L.), except for tolpyralate + glufosinate, which was antagonistic at 8 WAA. Tolpyralate + glufosinate was antagonistic for the control of barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] and Setaria spp. at 8 WAA. Common lambsquarters (Chenopodium album L.) control at 8 WAA was synergistic and ≥95% with mesotrione plus atrazine, bromoxynil, or glufosinate and with tolpyralate plus bromoxynil or bentazon. Herbicide tank mixes were generally additive for the control of wild mustard (Sinapis arvensis L.) at 8 WAA, except for the synergistic tank mixes of tolpyralate plus atrazine or bromoxynil; however, each tank mix provided 97% to 100% control of S. arvensis. Results from this study demonstrate that co-application of ROS generators with mesotrione or tolpyralate controlled all broadleaf weed species >90% at 8 WAA, with the exceptions of A. artemisiifolia and C. album control with tolpyralate + glufosinate. Mesotrione plus PSII inhibitors controlled E. crus-galli and Setaria spp. 48 to 68 percentage points less than tolpyralate plus the respective PSII inhibitor at 8 WAA; however, mesotrione + glufosinate and tolpyralate + glufosinate controlled the grass weed species similarly.

Ethylene Insensitive 3-Like 2 is a Brassicaceae-specific transcriptional regulator involved in fine-tuning ethylene responses in Arabidopsis thaliana.

Ethylene signaling directs a pleiotropy of developmental processes in plants. In Arabidopsis, ethylene signaling converges at the master transcription factor Ethylene Insensitive 3 (EIN3), which has five homologs, EIN3-like 1-5 (EIL1-EIL5). EIL1 is most fully characterized and operates similarly to EIN3, while EIL3-5 are not involved in ethylene signaling. EIL2 remains less investigated. Our phylogenetic analysis revealed that EIL2 homologs have only been retrieved in the Brassicaceae family, suggesting that EIL2 diverged to have specific functions in the mustard family. By characterizing eil2 mutants, we found that EIL2 is involved in regulating ethylene-specific developmental processes in Arabidopsis thaliana, albeit in a more subtle way compared with EIN3/EIL1. EIL2 steers ethylene-triggered hypocotyl elongation in light-grown seedlings and is involved in lateral root formation. Furthermore, EIL2 takes part in regulating flowering time as eil2 mutants flower on average 1 d earlier and have fewer leaves. A pEIL2:EIL2:GFP translational reporter line revealed that EIL2 protein abundance is restricted to the stele of young developing roots. EIL2 expression, and not EIL2 protein stability, is regulated by ethylene in an EIN3/EIL1-dependent way. Despite EIL2 taking part in several developmental processes, the precise upstream and downstream regulation of this ethylene- and Brassicaceae-specific transcription factor remains to be elucidated.

Acclimatization of the Growth of Brassica Juncea to Temperature Stress: Future of IoT Technology in Sustainable Agriculture

Agriculture and global warming are correlated with each other, particularly, it may affect nutrient cycles, microbial activities, and physiological activities of the crops. Agricultural development plays a crucial role in the growth of the economy of developing countries. The agriculture sector is a major source of employment in most of the developing countries. Over the year, there were changes and productivity loss due to the abiotic stresses and imbalance of nutrients of the plants. A continuous increase in temperature may affect the yields of crops up to 17%. Each plant has different characteristics in growth and some plants are susceptible to high temperature, some are quite the opposite. A Brassica Juncea L. belongs to a mustard family Brassicaceae or Cruciferae that are susceptible to high temperature. So, in this work, an attempt has been made for Brassica Juncea L. to grow and yield under temperature stress by controlling the temperature with the use of the Internet of Things (IoT). The experiment has been conducted where Brassica Juncea neither production nor consumption. IoT sensors are used to monitor the temperature and humidity in two different scenarios. This paper analyses the factors that affect the growth of Brassica Juncea and provide a solution to increase productivity.

Trapping of Crucifer-Feeding Flea Beetles (Phyllotreta spp.) (Coleoptera: Chrysomelidae) With Pheromones and Plant Kairomones.

Flea beetles (Coleoptera: Chrysomelidae) of the genus Phyllotreta are major pests of cole crops, canola, and related crops in the mustard family (Brassicaceae). Adults may damage seedlings or larger crop plants, impairing crop growth, rendering crops unmarketable, or killing seedlings outright. The two major North American crucifer pest species, Phyllotreta striolata (F.) and Phyllotreta cruciferae (Goeze), have male-produced pheromones attractive to both female and male adults. We tested the racemic synthetic pheromones, himachaladiene and hydroxyhimachalanone, as well as the host-plant-produced allyl isothiocyanate, alone and in combination, with experimental trapping in Maryland, Virginia, and North Dakota, using clear and yellow sticky traps and the ground-based 'rocket' trap (modified from boll weevil trap). Phyllotreta striolata was consistently attracted to the hydroxyketone, and captures were often enhanced by allyl isothiocyanate (AITC), but its response to pheromones, AITC, and trap color were variable from state to state. Phyllotreta cruciferae was strongly attracted to AITC, but its response to pheromone components varied by state, and this species was found rarely at the Maryland site. Phyllotreta bipustulata (F.) was attracted to the diene component, a new finding for this species. Several other genera of flea beetles were captured, some showing response to the semiochemicals and/or color. Results will be helpful in monitoring and possibly population suppression; however, further research is necessary to develop more efficient syntheses, optimal lure loadings, combinations, and controlled release methods.

Growth and yield of Sunflower (Helianthus annuus)in additive Intercropping system with Mung bean.

Some crops perform better in intercropping systems with other crops relative to their solcropping . Sunflower (Helianthus annuus L.) was adopted to be studied in a field experiment within an additive intercropping system with mungbean (Vigna radiata L.) in a three rows in combinations (1:3 and 1:4 and 1:5) sunflower-Mung bean intercropped and sole cropped for studding growth , yield components and yield traits during the summer of 2021 at two different agro ecological locations of the governorate of Erbil- Iraq. Experiments were triplicated in a randomized complete block design (RCBD), Each location was a parcel of a local farmers land in the villages of Shamamr (Latitude: 406227, Longitude: 397954 rainfall isocline (350 mm y-1)) and Dukalla (Latitude: 358033 m, Longitude: 3993384 m),at the last of the semi insured and the first of the non-insured rainfall zones respectively . Shamamr location suppressed Dukalla location in most of morphological traits at the ages of 20 days after sowing (DAS), nd 60 DAS. Treatment row combination (1:3, 1:4 and 1:5) showed advantages in yield component traits over sole cropping of sunflower might be due to resources gains as solar radiation, conditioning heat environment and space .No advantages of intercropping were observed among cropping systems in seed yield while the treatment (1:5) possessed the highest Harvest index (0.6). Sunflower in Shamamr location is believed to perform better under intercropping system. More planting geometry is recommended to obtain more precise results. References : Abràmoff, M.D., Magalhães, P.J. and Ram, S.J., 2004. Image processing with ImageJ. Biophotonics international, 11(7), pp.36-42. Anas, M., Jabbar, A., Sarwar, M.A., Ullah, R., Abuzar, M.K., Ijaz, A. and Latif, S., 2017. Intercropping Sunflower with Mung beanfor improved productivity and net economic return under irrigated conditions. Pakistan Journal of Agricultural Research, 30(4). Bindi, M. and Olesen, J.E., 2011. The responses of agriculture in Europe to climate change. Regional Environmental Change, 11(1), pp.151-158. Dizayyee , Aryan Suad Ahmed . (2019).Modes of Interference Compatibility of Wheat, Chickpea and Wild mustard Intercropping in Different Row Proportions. PhD Dissertation , University of Salahaddin , College of Agricultural Engineering Sciences. Gebru, H., 2015. A review on the comparative advantages of intercropping to mono-cropping system. Journal of Biology, Agriculture and Healthcare, 5(9), pp.1-13. Jeon, D.J., Ki, S.J., Cha, Y., Park, Y. and Kim, J.H., 2018. New methodology of evaluation of best management practices performances for an agricultural watershed according to the climate change scenarios: A hybrid use of deterministic and decision support models. Ecological Engineering, 119, pp.73-83.(abstract). Jubr Al-Layla, M. and Shahadha Khalid Al-Juheishy, W., 2012. EVALUATION OF DIFFERENT INTERCROPPING SYSTEM IN SUNFLOWER (Helianthus annuus L.) ANDMUNGBEAN (Vigna radiata L.). Mesopotamia Journal of Agriculture, 40, pp.62-74. Khan, M. N., Shoaib Muhammad, Mahboob Asrar, M. S. Ashraf, and Qamar Rafi. "MB (Vigna radiata) intercropping enhances productivity of late season irrigated cotton in Punjab." Asian Journal of Agriculture and Biology 8, no. 4 (2020): 472-479. Lithourgidis, A.S., Dordas, C.A., Damalas, C.A. and Vlachostergios, D., 2011. Annual intercrops: an alternative pathway for sustainable agriculture. Australian journal of crop science, 5(4), pp.396-410. Mousavi, S.R. and Eskandari, H., 2011. A general overview on intercropping and its advantages in sustainable agriculture. Journal of Applied Environmental and Biological Sciences, 1(11), pp.482-486. Nawar, A.I., Salama, H.S. and Khalil, H.E., 2020. Additive intercropping of SF and soybean to improve yield and land use efficiency: Effect of thinning interval and nitrogen fertilization. Chilean journal of agricultural research, 80(2), pp.142-152. Shi, H., Tian, H., Lange, S., Yang, J., Pan, S., Fu, B. and Reyer, C.P., 2021. Terrestrial biodiversity threatened by increasing global aridity velocity under high-level warming. Proceedings of the National Academy of Sciences, 118(36). Vandermeer, J.H., 1992. The ecology of intercropping. Cambridge University Press. (book) .

Effects of an introduced mustard, Thlaspi arvense, on soil fungal communities in subalpine meadows

The subalpine meadows of the Rocky Mountains, USA, are at the advancing front of global change; however, little is known about the sensitivities of high-elevation soil fungal communities to ongoing ecological changes. Soil fungi are sensitive to abiotic and biotic environmental stressors, including climate change, soil disturbance, and the presence of introduced, non-native plants. Invasive plants in the Brassicaceae (mustard family) are known to alter fungal community structure, suppress arbuscular mycorrhizal fungi, and change their relationship with native plant hosts in forest ecosystems, but these phenomena have not been studied in the subalpine zone where non-native mustard plants are becoming established. Here, we investigated whether the presence of the introduced mustard plant, Thlaspi arvense , is associated with distinct properties of the whole fungal and arbuscular mycorrhizal fungal communities in subalpine meadow ecosystems. We observed clear differences in the composition, relative abundance of core taxa, and mean taxon relatedness of soil fungal communities in plots with T. arvense relative to those with only native vegetation. A suite of novel fungi were associated with T. arvense , and overall patterns of AMF phylogenetic diversity were drastically reduced in association with its presence. Our results suggest that T. arvense introduction impacts the soil fungal community, with potential implications for native plant communities and soil nutrient cycling in high elevation meadows of the Rocky Mountains.

Genetic analysis reveals three novel QTLs underpinning a butterfly egg-induced hypersensitive response-like cell death in Brassica rapa.

BackgroundCabbage white butterflies (Pieris spp.) can be severe pests of Brassica crops such as Chinese cabbage, Pak choi (Brassica rapa) or cabbages (B. oleracea). Eggs of Pieris spp. can induce a hypersensitive response-like (HR-like) cell death which reduces egg survival in the wild black mustard (B. nigra). Unravelling the genetic basis of this egg-killing trait in Brassica crops could improve crop resistance to herbivory, reducing major crop losses and pesticides use. Here we investigated the genetic architecture of a HR-like cell death induced by P. brassicae eggs in B. rapa.ResultsA germplasm screening of 56 B. rapa accessions, representing the genetic and geographical diversity of a B. rapa core collection, showed phenotypic variation for cell death. An image-based phenotyping protocol was developed to accurately measure size of HR-like cell death and was then used to identify two accessions that consistently showed weak (R-o-18) or strong cell death response (L58). Screening of 160 RILs derived from these two accessions resulted in three novel QTLs for Pieris brassicae-induced cell death on chromosomes A02 (Pbc1), A03 (Pbc2), and A06 (Pbc3). The three QTLs Pbc1–3 contain cell surface receptors, intracellular receptors and other genes involved in plant immunity processes, such as ROS accumulation and cell death formation. Synteny analysis with A. thaliana suggested that Pbc1 and Pbc2 are novel QTLs associated with this trait, while Pbc3 also contains an ortholog of LecRK-I.1, a gene of A. thaliana previously associated with cell death induced by a P. brassicae egg extract.ConclusionsThis study provides the first genomic regions associated with the Pieris egg-induced HR-like cell death in a Brassica crop species. It is a step closer towards unravelling the genetic basis of an egg-killing crop resistance trait, paving the way for breeders to further fine-map and validate candidate genes.

Enhancement of tolpyralate efficacy with adjuvants

AbstractTolpyralate is a 4-hydroxyphenylpyruvate dioxygenase–inhibiting herbicide that is applied postemergence for control of annual broadleaf and grass weeds in corn. Current Canadian label recommendations for tolpyralate specify the addition of a methylated seed oil (MSO) adjuvant (MSO Concentrate®) for improved weed control. The efficacy of tolpyralate applied with other proprietary adjuvants has not been widely reported in the peer-reviewed literature. Therefore, four field trials were conducted in corn over 2020 and 2021 in Ontario, Canada, to evaluate MSO Concentrate®, Agral® 90 (nonionic surfactant), Assist® Oil Concentrate (blended surfactant), Carrier® (blended surfactant), LI 700® (nonionic surfactant), and Merge® (blended surfactant) as adjuvants with tolpyralate for the control of annual broadleaf and grass weeds. At 8 wk after application (WAA), tolpyralate applied with MSO Concentrate®, Agral® 90, Assist® Oil Concentrate, Carrier®, or Merge® controlled velvetleaf, wild mustard, barnyardgrass, and foxtail species similarly. These adjuvants also enhanced the efficacy of tolpyralate similarly for the control of common ragweed at 8 WAA with the exception that Agral® 90 was inferior to Merge®. At 8 WAA, tolpyralate controlled common lambsquarters the greatest when applied with MSO Concentrate®, Agral® 90, Carrier®, or Merge®; these adjuvants with the exception of Agral® 90 were superior to Assist® Oil Concentrate. At 8 WAA, tolpyralate applied with LI 700® controlled common ragweed, barnyardgrass, and foxtail species less than when tolpyralate was applied with the other adjuvants tested; control of these weed species with tolpyralate was not improved with LI 700® when compared to tolpyralate applied without an adjuvant. Overall, tolpyralate applied with either MSO Concentrate®, Carrier®, or Merge® controlled all annual broadleaf and grass weed species similarly or greater than tolpyralate applied without an adjuvant or tolpyralate with Agral® 90, Assist® Oil Concentrate, or LI 700® at 8 WAA.

Enhancement of Tolpyralate + Bromoxynil Efficacy With Adjuvants in Corn

The most effective adjuvant is often herbicide-specific and dependent on the target weed species. Tolpyralate is a 4-hydroxyphenylpyruvate dioxygenase-inhibiting herbicide that is usually tank-mixed with atrazine for improved weed control in corn (Zea mays L.). Previous research has reported that a methylated seed oil (MSO) adjuvant (MSO Concentrate®) enhances the efficacy of tolpyralate + atrazine on several annual broadleaf and grass weed species. The efficacy of tolpyralate can also be improved with the addition of bromoxynil instead of atrazine; however, there is no information in the peer-reviewed literature that tests the efficacy of tolpyralate + bromoxynil with different adjuvants. Therefore, four field trials were conducted in 2020 and 2021 in Ontario, Canada to evaluate the efficacy of tolpyralate + bromoxynil without an added adjuvant and with MSO Concentrate®, Agral® 90, Assist® Oil Concentrate, Carrier®, LI 700®, or Merge® on six annual weed species in corn. The adjuvants did not enhance control of wild mustard (Sinapis arvensis L.) or velvetleaf (Abutilon theophrasti Medik.) with tolpyralate + bromoxynil. At 8 weeks after application (WAA), common ragweed (Ambrosia artemisiifolia L.) control was improved with the addition of MSO Concentrate® or Merge® to tolpyralate + bromoxynil. At 8 WAA, all adjuvants enhanced the control of common lambsquarters (Chenopodium album L.) with tolpyralate + bromoxynil similarly except for Assist® Oil Concentrate; Merge® was a better adjuvant than Assist® Oil Concentrate for common lambsquarters control. At 8 WAA, control of barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] was enhanced with the addition of Assist® Oil Concentrate, Carrier®, MSO Concentrate®, or Merge® to tolpyralate + bromoxynil. Carrier®, MSO Concentrate®, and Merge® enhanced the efficacy of tolpyralate + bromoxynil on Setaria spp. at 8 WAA. Control of velvetleaf, wild mustard, common ragweed, Setaria spp., and barnyardgrass with tolpyralate + bromoxynil was similar with the six adjuvants evaluated at 8 WAA.

Enhancement of tolpyralate + atrazine efficacy with adjuvants

AbstractTolpyralate is commonly mixed with atrazine for improved control of common annual weed species in corn production systems in the United States and Canada. Weed control efficacy with this mixture is enhanced with the addition of methylated seed oil (MSO) Concentrate®; however, there is little information on the efficacy of tolpyralate + atrazine with other proprietary adjuvants. Therefore, four trials were conducted at field research sites in Ontario, Canada, to evaluate the efficacy of tolpyralate + atrazine when applied with six different commercially available adjuvants on four annual broadleaf and two annual grass weed species in corn. The adjuvants evaluated were MSO Concentrate®, Agral® 90, Assist® Oil Concentrate, Carrier®, LI 700®, and Merge®. A treatment of tolpyralate + atrazine applied with no adjuvant was also included in the study. For the control of velvetleaf and wild mustard, the adjuvants evaluated with tolpyralate + atrazine did not improve control. At 8 wk after application (WAA), the use of Agral® 90, Assist® Oil Concentrate, Carrier®, MSO Concentrate®, or Merge® with tolpyralate + atrazine provided similar or greater control of common ragweed than tolpyralate + atrazine applied with LI 700®. At 8 WAA, the adjuvants performed similarly with tolpyralate + atrazine for the control of common lambsquarters; however, LI 700® was the only adjuvant that did not improve control compared to tolpyralate + atrazine applied without an adjuvant. At 8 WAA, MSO Concentrate®, Carrier®, and Merge® improved control of barnyardgrass and foxtail species with tolpyralate + atrazine to a similar or greater level than Assist® Oil Concentrate, Agral® 90, and LI 700®. Overall, MSO Concentrate®, Carrier®, or Merge® should be added to tolpyralate + atrazine for control of the myriad of weed species interfering with corn production.

Variation of the seed endophytic bacteria among plant populations and their plant growth-promoting activities in a wild mustard plant species, Capsella bursa-pastoris.

Recent studies have revealed that some bacteria can inhabit plant seeds, and they are likely founders of the bacterial community in the rhizosphere of or inside plants at the early developmental stage. Given that the seedling establishment is a critical fitness component of weedy plant species, the effects of seed endophytic bacteria (SEB) on the seedling performance are of particular interest in weed ecology. Here, we characterized the SEB in natural populations of Capsella bursa‐pastoris, a model species of weed ecology. The composition of endophytic bacterial community was evaluated using deep sequencing of a 16S rDNA gene fragment. Additionally, we isolated bacterial strains from seeds and examined their plant growth‐promoting traits. Actinobacteria, Firmicutes, Alpha‐, and Gammaproteobacteria were major bacterial phyla inside seeds. C. bursa‐pastoris natural populations exhibited variable seed microbiome such that the proportion of Actinobacteria and Alphaproteobacteria differed among populations, and 60 out of 82 OTUs occurred only in a single population. Thirteen cultivable bacterial species in six genera (Bacillus, Rhodococcus, Streptomyces, Staphylococcus, Paenibacillus, Pseudomonas) were isolated, and none of them except Staphylococcus haemolyticus were previously reported as seed endophytes. Eight isolates exhibited plant growth‐promoting traits like phosphate solubilization activity, indole‐3‐acetic acid, or siderophore production. Despite the differences in the bacterial communities among plant populations, at least one isolated strain from each population stimulated shoot growth of either C. bursa‐pastoris or its close relative A. thaliana when grown with plants in the same media. These results suggest that a weedy plant species, C. bursa‐pastoris, contains bacterial endophytes inside their seeds, stimulating seedling growth and thereby potentially affecting seedling establishment.

Hybridization and complex evolution of Barbarea vulgaris and related species (Brassicaceae)

Barbarea, winter-cress, is a genus of 29 species in Brassicaceae, the mustard family, which has emerged as a model for evolution of plant defence and specialised metabolites. Notably, some Barbarea species have evolved the ability to produce triterpenoid saponins as the only ones in Brassicaceae, some of which make plants resistant to important herbivores. Resistance has, however, been lost in a distinct group of plants within B. vulgaris ssp. arcuata, which is genetically strongly diverged from other B. vulgaris plants. This divergence is not reflected present in taxonomy. Thus, a phylogeny is needed to understand evolution and defence in Barbarea. Here, we analysed the nuclear ITS and the plastid matK, ndhF, rps16, and psbA-trnH DNA regions from seven out of 29 Barbarea species, 57 accessions of B. vulgaris, 10 accessions of other Barbarea species, and eight outgroup species, in addition to sequences available from GenBank. All Barbarea species formed a highly supported monophyletic group, separated from sister genera. Several clades seem to have radiated within the genus with no simple branching pattern, and discordant nuclear and plastid DNA phylogenies indicate reticulate evolution and chloroplast capture. One of the complex patterns may have resulted from chloroplast capture of a non-Nordic Barbarea species not included in the study. Two pairs of species were almost identical, B. australis and B. grayi, and B. orthoceras and B. stricta. Despite hybridization, chloroplast capture, and incongruence among the plastid and nuclear DNA data, the high level of intraspecific diversity, coupled with lineage specificity, lead us to recognize three groups of Barbarea vulgaris: G-type (glabrous) and P-type (pubescent) individuals of the current B. vulgaris ssp. arcuata as two distinct groups and the current B. vulgaris ssp. vulgaris as the third. Despite the high molecular diversity below species level, the evolutionary history of the saponin-based resistance remains unsettled due to unresolved basal branching.

The role of acidic, alkaline and hydrothermal pretreatment on pyrolysis of wild mustard (Sinapis arvensis) on the properties of bio-oil and bio-char

In this study, the biomass (Sinapis arvensis) was pretreated with acidic (H2SO4, HCI), alkaline (NaOH, KOH) and high temperature water (HTW) after this samples pyrolyzed at 350, 450 and 550 °C. GC–MS, FT-IR, Elemental, 1H NMR and SEM analysis methods were used in the characterization of the products obtained at the end of the pyrolysis process. It has been determined that alkaline and acidic pretreatment increase the liquid product yield and also support the formation of high energy value biochar. According to the liquid product content obtained as a result of pyrolysis, HTW and alkaline pretreatments are more effective in the formation of mono aromatics, and acidic pretreatments are more effective in the production of aliphatic.

Cruciferous Weeds Do Not Act as Major Reservoirs of Inoculum for Black Rot Outbreaks in New York State.

Cruciferous weeds have been shown to harbor diverse Xanthomonas campestris pathovars, including the agronomically damaging black rot of cabbage pathogen, X. campestris pv. campestris. However, the importance of weeds as inoculum sources for X. campestris pv. campestris outbreaks in New York remains unknown. To determine if cruciferous weeds act as primary reservoirs for X. campestris pv. campestris, fields that were rotating between cabbage or had severe black rot outbreaks were chosen for evaluation. Over a consecutive 3-year period, 148 cruciferous and noncruciferous weed samples were collected at 34 unique sites located across five New York counties. Of the 148 weed samples analyzed, 48 X. campestris isolates were identified, with a subset characterized using multilocus sequence analysis. All X. campestris isolates originated from weeds belonging to the Brassicaceae family, with predominant weed hosts being shepherd's purse (Capsella bursa-pastoris), wild mustard (Sinapis arvensis), yellow rocket (Barbarea vulgaris), and pennycress (Thlaspi arvense). Identifying pathogenic X. campestris weed isolates was rare, with only eight isolates causing brown necrotic leaf spots or typical V-shaped lesions on cabbage. There was no evidence of cabbage-infecting weed isolates persisting in an infected field by overwintering in weed hosts; however, similar cabbage and weed X. campestris haplotypes were identified in the same field during an active black rot outbreak. X. campestris weed isolates are genetically diverse both within and between fields, but our findings indicate that X. campestris weed isolates do not appear to act as primary sources of inoculum for B. oleracea fields in New York.

The Role of Acidic, Alkaline and Hydrothermal Pretreatment on Pyrolysis of Wild Mustard (Sinapis Arvensis) on the Properties of Bio-Oil and Bio-Char

The Role of Acidic, Alkaline and Hydrothermal Pretreatment on Pyrolysis of Wild Mustard (Sinapis Arvensis) on the Properties of Bio-Oil and Bio-Char

Toxic Effects of Treatment with Cyclophosphamide on Serum Biochemical Markers of Hepatic Function in Albino Rats

Despite being an effective modality of treatment against cancer, chemotherapy is a major factor responsible for the morbidity and mortality caused by cancer. Cyclophosphamide, a widely used chemotherapeutic drug belonging to the Nitrogen Mustard family of alkylating agents, has been documented for many side- effects on the healthy tissues and metabolism of individuals undergoing treatment. In the present study, an effort has been made to investigate the effects of cyclophosphamide administration on hepatic metabolism of albino rats. Male albino rats were divided into two groups (n= 6 in each). Group 1 (Vehicle control) rats were treated with distilled water and group II rats were intraperitoneally administered with single dose of cyclophosphamide at the dose of 200 mg/kg b wt on day 1 of the experiment. Blood was collected from rats of each group on day 7 via cardiac puncture. Serum was separated and analysed for level of liver function markers and lipid profile parameters. Intraperitoneal administration of cyclophosphamide resulted in a significant increase in serum level of bilirubin and liver enzymes-- Serum Glutamic Pyruvic Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT) and Alkaline Phosphatase (ALP) (P<0.001), while decreased the level of total protein (P<0.01), albumin (P<0.05) and globulin, when compared to vehicle control group rats. A significant increment in serum level of Total Cholesterol (TC), Low Density Lipoprotein (LDL-c), Triglycerides (TG) and Very Low Density Lipoprotein (VLDL- c) along with a significant decrease in High Density Lipoprotein (HDL-c) was observed following cyclophosphamide administration in group II rats, when compared to control group (P<0.001)Cyclophosphamide caused severe biochemical alterations resulting in hepatic toxicity, increased lipid peroxidation and redox imbalances in albino rats. Therefore, a routine monitoring of liver function might be advisable for the individuals undergoing chemotherapy as well as before recommending the chemotherapy session.

کمی‌سازی جوانه‌زنی بذر علف‌های‌هرز خانواده براسیکاسه (Brassicaceae) تحت شرایط شوری و خشکی

Introduction Knowledge on germination ecology of weeds in response to salinity and drought stresses can help to predict weeds population dynamics in the future. Among the family of weeds, the weeds of Brassicaceae family include many important species that contaminate crops and orchards. In addition to the presence of weeds, salinity and drought are also considered as limiting factors in agricultural production. Due to the increase in salinity and drought stresses in agricultural fields, it can be significant expansion and contamination of Brassicaceae weeds, because Physiological dormancy, abundant seed production and emergence at different time, leads to be persistent for long-term in soil. Therefore, the aim of this research was to compare the characteristics of seed germination of four weeds Brassicaceae family to salinity and drought stresses. Materials and methods In order to investigate the effects of salinity (0, 50, 100, 150, 200, 250, 300, 350 and 400 Mm) and drought stress (0, -0.2, -0.4, -0.6, -0.8, -1 and -1.2 MPa) on seed germination four Brassicaceae family weeds (Rocket (Eruca sativa), Hoary Mustard (Hirschfeldia incana), Wild Mustard (Sinapis arvensis) and Treacle Mustard (Erysimum repandum), two experiments as factorial based on completely randomized design (CRD) were conducted at agricultural sciences and natural resources university of Khuzestan in 2019 with three replications. Optimum Temperature for germination were selected 25°C for Rocket, Hoary Mustard, Wild Mustard and 15°C for Treacle Mustard. Duration of test for two experiments was considered 15 days. Criterion for germination was length radicle 2-3 mm. Results and discussion By increasing drought stress, germination, vigour index and germination rate were decreased and no germination was observed in drought stress of -1.2 MPa. 50% reduction for germination in four weeds Rocket, Hoary Mustard, Wild Mustard and Treacle Mustard were estimated in -1, -0.49, -0.76 and – 0.41 MPa of drought stress respectively. Drought stress required to reduce 50% of germination rate for Rocket, Hoary Mustard, Wild Mustard and Treacle Mustard were estimated -0.85, -0.35, -0.28 and 0.22 MPa respectively.The vigour index of Wild Mustard in conditions without drought stress treatment was more than other weeds. Rocket and Hoary Mustard were introduced as resistant and Wild Mustard was introduced as sensitive to salinity. The salinity required to reduce 50% of germination for Rocket, Hoary Mustard, Wild Mustard and Treacle Mustard were predicted 267, 162, 39 and 46 mM, while 50% reduction for germination rate were 174, 142, 27, 44 Mm respectively. By increasing salinity and drought stresses, mean germination time was increased and maximum mean germination time belonged to Treacle Mustard and the reason for this increase was the lag phase of germination. Germination rate in four weeds was more affected by salinity and drought stresses than germination percentage. Generally the germination rate of Treacle Mustard was much less than other weeds. So that the maximum germination rate of Treacle Mustard was 5 seed/day in control treatment while in Rocket Hoary Mustard, Wild Mustard were more than 30 seed /day. Conclusions According to the results, in saline lands, the spread of two weeds Rocket and Hoary Mustard is more expected and in the conditions of drought stress and in rainfed agriculture, presence of Rocket is more evident than Wild Mustard.

Plant age at the time of ozone exposure affects flowering patterns, biotic interactions and reproduction of wild mustard

Exposure of plants to environmental stressors can modify their metabolism, interactions with other organisms and reproductive success. Tropospheric ozone is a source of plant stress. We investigated how an acute exposure to ozone at different times of plant development affects reproductive performance, as well as the flowering patterns and the interactions with pollinators and herbivores, of wild mustard plants. The number of open flowers was higher on plants exposed to ozone at earlier ages than on the respective controls, while plants exposed at later ages showed a tendency for decreased number of open flowers. The changes in the number of flowers provided a good explanation for the ozone-induced effects on reproductive performance and on pollinator visitation. Ozone exposure at earlier ages also led to either earlier or extended flowering periods. Moreover, ozone tended to increase herbivore abundance, with responses depending on herbivore taxa and the plant age at the time of ozone exposure. These results suggest that the effects of ozone exposure depend on the developmental stage of the plant, affecting the flowering patterns in different directions, with consequences for pollination and reproduction of annual crops and wild species.

Wild Mustard (Sinapis arvensis) Competition and Control in Rain-Fed Spring Wheat (Triticum aestivum L.)

Wild mustard (Sinapis arvensis L.) is a weed that frequently infests spring wheat (Triticum aestivum L.) fields in Moscow province, Russia. It is an annual broad leaf weed, which is indigenous throughout most parts of the globe and one of the most competitive weeds of spring cereal crops. In southern Russia it is emerging as an important crop competitor. Field trials focusing on herbicide timing and efficacy on wild mustard control and spring wheat yield in the Moscow region, Kashira and Baribino districts. A PRE glyphosate application to wheat regardless of fall or spring application timing favorably suppressed wild mustard in 2018. Weeds were not controlled in 2019 with the earliest application timings of glyphosate because weeds emerged late. In comparing fall and spring application timings, the formulated combination of (iodosulfuron/mesosulfuron/antidote mefenpyr-diethyl) at both field rates provided 80% weed control for all application timings and locations, and also resulting in the greatest spring wheat grain yield. Overall, herbicide treatments performed greater when they were in the fall than during the spring. Based on POST herbicide application, tribenuron-methyl provided the greatest wild mustard suppression (75%) and also caused the highest reduction in wild mustard biomass (3.3 g), stem number (6), seed number (880) and germination percentage (33%). When wild mustard was approximately 32 weeds/m2 causedtotal wheat yield loss.