Vision is an essential element for maintaining the balance in the standing position or for walking. The purpose of this study was to investigate the effects of dominant or non-dominant eye blocking on gait and balance. In 17 healthy individuals, static balance and gait function were measured when the dominant or non-dominant eye were blocked. Static balance was measured by the Wii balance board and the Balancia software program. We also measured gait function (spatio-temporal variables) and foot lateral deviations. In the present results, the path length, path velocity, and path area were significantly decreased during blocking dominant on static balance function (p<0.05). There were no significant differences on all gait parameters except velocity during blocking the non-dominant or dominant eye. We could confirm that the use of monocular with non-dominant eye can help improve static balance ability, but it is meaningless except for velocity during gait with monocular.