Wide Ring Research Articles

Characterization of Macular Fundus Autofluorescence Changes in Patients with Retinitis Pigmentosa.

To characterize fundus autofluorescence (AF) changes that occur in the macula of patients with retinitis pigmentosa (RP). We conducted a case series on ninety-nine patients with RP. Features seen on fundus AF images were evaluated, organized into a grading scheme and correlated with ellipsoid zone (EZ) width. Patterns of AF changes occurring in the macula and correlation with EZ width. Four primary fundus AF phenotypes were identified: (1) hyperAF arc, (2) hyperAF ring, (3) hyperAF ring with abnormal hyperAF within the ring, and (4) central hyperAF. The second phenotype was most common and had 3 subgroups, hyperAF rings within the macula, those extending outside the macula, and incomplete rings. HyperAF rings were also characterized as narrow or wide with wide rings having a greater amount of hyperAF. Linear Mixed-Effects Model showed mean measured EZ width was significantly greater for phenotype 1 versus each of the other 3 phenotypes (p<0.01) and for phenotype 2 versus phenotypes 3 and 4 (p<0.05), and also differed among phenotype 2 subgroups (p<0.05). Other AF characteristics identified were focal posterior distinct or indistinct hypoAF which sometimes formed complete or incomplete hypoAF rings surrounding a hyperAF ring, diffuse or focal hyperAF outside hyperAF rings, and the amount of encroachment of peripheral hypoAF. A grading scheme for macular AF features in patients with RP identified phenotypes that correlate with stage of disease based upon EZ width. Longitudinal studies are needed to test whether presumed early AF phenotypes evolve into later phenotypes. Use of the grading scheme for patient populations in interventional trials could help to determine if any of the defined AF features provide predictive value for therapeutic responses.

Links between tree phenology and wood traits in sessile oak (Quercus petraea (Matt.) Liebl.)

Tree phenology and wood traits are considered of foremost importance in understanding species’ responses to the influence of climate change, thus the existence of a link between tree phenology and wood traits has become increasingly appealing in forestry research. The aim of this study was to explore the relationship between leaf flushing and wood traits (basic wood density, tree ring width, tree ring phenology, sapwood width and number of sapwood rings) in sessile oak (Quercus petraea (Matt.) Liebl.). We investigated trees with distinctive phenological behaviour (early flushing and late flushing) from three peripheral populations, one non-peripheral population and one sessile oak comparative trial close to the species eastern distribution range in the Eastern Carpathians. Covariance analysis, partial correlations, nonparametric statistics, and Principal Component Analysis were used to investigate the link between tree phenology, wood density, and some structural metrics. It was statistically confirmed that, at comparable ages and radial growth, the sessile oak trees exhibiting late leaf flushing had lighter basic wood density and greater density differences between sapwood and heartwood than early flushing trees. Independent of age, trees with intermediate flushing have wider rings, the formation of which is much earlier than in early flushing trees. At the same age, late trees from geographically peripheral populations have a higher number of sapwood rings. Also, the results revealed that peripheral populations had lower basic wood density, while this was not the case for peripheral provenances tested in the comparative trail established in an ecologically optimal area. These findings help us enlarge our understanding of the relationship between leaf flushing and wood traits, which could be useful for improving sessile oak management strategies near the eastern limit of the sessile oak natural range, which is becoming more and more critical in the context of the current climate changes.

Dust ring and gap formation by gas flow induced by low-mass planets embedded in protoplanetary disks

The observed dust rings and gaps in protoplanetary disks could be imprints of forming planets. Even low-mass planets in the 1-10 Earth-mass regime, which have not yet carved deep gas gaps, can generate such dust rings and gaps by driving a radially-outward gas flow, as shown in previous work. However, understanding the creation and evolution of these dust structures is challenging due to dust drift and diffusion, requiring an approach beyond previous steady state models. Here we investigate the time evolution of the dust surface density influenced by the planet-induced gas flow, based on post-processing three-dimensional hydrodynamical simulations. We find that planets larger than a dimensionless thermal mass of m = 0.05, corresponding to 0.3 Earth mass at 1 au or 1.7 Earth masses at 10 au, generate dust rings and gaps, provided that solids have small Stokes numbers (St ≲ 10−2) and that the disk midplane is weakly turbulent (αdiff ≲10−4). As dust particles pile up outside the orbit of the planet, the interior gap expands with time when the advective flux dominates over diffusion. Dust gap depths range from a factor of a few to several orders of magnitude, depending on planet mass and the level of midplane particle diffusion. We constructed a semi-analytic model describing the width of the dust ring and gap, and then compared it with the observational data. We find that up to 65% of the observed wide-orbit gaps could be explained as resulting from the presence of a low-mass planet, assuming αdiff = 10−5 and St = 10−3. However, it is more challenging to explain the observed wide rings, which in our model would require the presence of a population of small particles (St = 10−4). Further work is needed to explore the role of pebble fragmentation, planet migration, and the effect of multiple planets.

Effects of different interlayer bonding modes on the microstructure and properties of laser cladded In625

In this study, the effects of five different interlayer bonding modes on the microstructure, mechanical properties, friction, and wear resistance of In625 laser cladding were investigated. XRD, EDS, tensile, hardness, friction and wear tests were conducted. XRD analysis results have revealed that Nb6C5 was detected in rectangular ring mode and the long, wide and rectangular ring overlay mode, indicating that the rectangular ring mode may promote the formation of secondary phase. EDS analysis has indicated a slightly higher Nb content in the rectangular ring mode, while the long and wide direction overlay mode exhibited a more uniform grain distribution and smaller grain size. Tensile tests revealed ductile fractures in the long direction, wide direction, the long and wide direction overlay modes, while the rectangle ring mode and the long, wide, and rectangular ring overlay modes showed brittle fractures. The long and wide direction overlay mode had the highest average elongation (35.46 %), and the long, wide, and rectangular ring overlay mode had the highest tensile strength (957.13 MPa). Hardness test results showed that the rectangle ring mode had the highest hardness (325.1 HV (0.2)). Friction and wear tests indicated that the long and wide direction overlay mode exhibited the smallest wear volume (0.08899 mm3) and the best overall friction performance. These findings indicate that the properties of the cladding layer can be effectively tailored by selecting appropriate interlayer bonding modes to match the specific material requirements in distinct application scenarios.

Shadows Wreak Havoc in Transition Disks

We demonstrate that shadows cast on a protoplanetary disk can drive it eccentric. Stellar irradiation dominates heating across much of these disks, so an uneven illumination can have interesting dynamical effects. Here, we focus on transition disks. We carry out 3D Athena++ simulations, using a constant thermal relaxation time to describe the disk’s response to changing stellar illumination. We find that an asymmetric shadow, a feature commonly observed in real disks, perturbs the radial pressure gradient and distorts the fluid streamlines into a set of twisted ellipses. Interactions between these streamlines have a range of consequences. For a narrow ring, an asymmetric shadow can sharply truncate its inner edge, possibly explaining the steep density drop-offs observed in some disks and obviating the need for massive perturbers. For a wide ring, such a shadow can dismantle it into two (or possibly more) eccentric rings. These rings continuously exert torque on each other and drive gas accretion at a healthy rate, even in the absence of disk viscosity. Signatures of such twisted eccentric rings may have already been observed as, e.g., twisted velocity maps inside gas cavities. We advocate for more targeted observations and for a better understanding on the origin of such shadows.

Wide Frequency Multiphase Oscillator Designed by Coupling Two Identical Ring Voltage Controlled Oscillators

ABSTRACTThis work presents a novel wide tuning range ring voltage controlled oscillator (RVCO) designed to generate six‐phase signals. The proposed circuit is realized by coupling two identical three‐stage single‐ended inverter‐based RVCOs. The coupling network injects the common mode signals from one RVCO to the other one and vice versa in a cross‐connected manner. Three‐stage RVCOs used for coupling have been configured by modifying the conventional current‐starved RVCO. The proposed six‐phase RVCO is optimized using an improved version of non‐dominated Sorting Genetic Algorithm III (NSGAIII). In this algorithm, the sigma scaling method is used to control selection pressure and enhance the algorithm's performance. The proposed RVCO oscillates at 3.46 GHz. It can operate from 970 MHz to 4 GHz with a supply voltage of 1.5 V, demonstrating a wide frequency tuning range of 120%. Additionally, another six‐phase oscillator is proposed, achieved by capacitively coupling two identical three‐stage RVCOs. Both proposed oscillators are designed in TSMC 0.18 μm CMOS technology.

A deep learning-informed interpretation of why and when dose metrics outside the PTV can affect the risk of distant metastasis in SBRT NSCLC patients

PurposeRecent papers suggested a correlation between the risk of distant metastasis (DM) and dose outside the PTV, though conclusions in different publications conflicted. This study resolves these conflicts and provides a compelling explanation of prognostic factors.Materials and methodsA dataset of 478 NSCLC patients treated with SBRT (IMRT or VMAT) was analyzed. We developed a deep learning model for DM prediction and explainable AI was used to identify the most significant prognostic factors. Subsequently, the prognostic power of the extracted features and clinical details were analyzed using conventional statistical methods.ResultsTreatment technique, tumor features, and dosiomic features in a 3 cm wide ring around the PTV (PTV3cm) were identified as the strongest predictors of DM. The Hazard Ratio (HR) for Dmean,PTV3cm was significantly above 1 (p < 0.001). There was no significance of the PTV3cm dose after treatment technique stratification. However, the dose in PTV3cm was found to be a highly significant DM predictor (HR > 1, p = 0.004) when analyzing only VMAT patients with small and spherical tumors (i.e., sphericity > 0.5).ConclusionsThe main reason for conflicting conclusions in previous papers was inconsistent datasets and insufficient consideration of confounding variables. No causal correlation between the risk of DM and dose outside the PTV was found. However, the mean dose to PTV3cm can be a significant predictor of DM in small spherical targets treated with VMAT, which might clinically imply considering larger PTV margins for smaller, more spherical tumors (e.g., if IGTV > 2 cm, then margin ≤ 7 mm, else margin > 7 mm).

THE effect of P2X7 receptor activation on functional responses of human left internal mammary artery.

The Purinoreceptor 7 (P2X7R) has become a promising drug target in many cardiovascular diseases, including coronary artery disease, since prolonged activation of P2X7R could promote vascular dysfunction, atherosclerosis, and thrombosis. Thus, we aimed to study the effects of P2X7R activation on vascular relaxation responses of the human left internal mammary artery (LIMA). Sections of redundant human LIMA were cut into 3-mm wide rings,, suspended in 20-mL organ baths containing physiologic salt solution, and attached to an isometric force transducer connected to a computer-based data acquisition system. Long-term (60min) incubation with specific P2X7R agonist Bz-ATP caused significant reductions in relaxation responses of LIMA to ATP and acetylcholine, which were reversed by selective P2X7R antagonists Brilliant Blue G or AZ11645373, whereas there were no changes in relaxation responses to endothelium-independent vasodilators isoprenaline, cAMP analog 8-Br-cAMP, and nitric oxide donor sodium nitroprusside. The impairment in relaxant responses of LIMA to endothelium-dependent vasodilators following activation of P2X7R for the long-term may contribute to postoperative LIMA vasospasm and hypertension. Modulation of P2X7R activity with selective agents may represent a new potential therapeutic approach in patients undergoing coronary artery bypass grafting surgery.

Leaf phenology and paleoclimatic and paleoenvironmental insights derived from Yiwupitys elegans of the Yiwu Jurassic Forest, Xinjiang, China

Many permineralized conifer stems and trunks have been documented from the Jurassic strata of the Xinjiang Uygur Autonomous Region (Xinjiang), Northwest China. Xylem anatomical studies have showed that these stems and trunks belonged to a diverse range of plants. However, the leaf phenology and the paleoclimatic and paleoenvironmental significance of these fossil plants have rarely been studied. We quantitatively analyzed the anatomical characteristics of a permineralized conifer stem—Yiwupitys elegans Gou et Feng 2023—from the Middle Jurassic Xishanyao Formation in northeastern Xinjiang, which has distinct and wide growth rings in the secondary xylem. Analyses of the Mean Sensitivity and Ring Markedness Index of the growth rings suggest that a stable paleoclimate with regular seasonal fructifications likely prevailed during the Middle Jurassic in this area. The statistical analyses of cell diameters and anatomical features of leaf traces in six successive growth rings indicate that this fossil plant was deciduous. Our study elucidates the ecophysiological characteristics of a Middle Jurassic conifer plant and provides new insights into the corresponding paleoclimate and paleoenvironment based on quantitative cellular analysis of the fossil stem.

A wide tuning range CMOS differential ring VCO using an active inductor for wireless applications

AbstractA differential ring voltage‐controlled oscillator (DRVCO) is proposed in this paper as one of the critical blocks in communication systems. It consists of four stages of delay cells connected in a chain, creating a ring structure with auxiliary path interconnections. The oscillation frequency of the DRVCO can be controlled by adjusting the tuning voltage that controls the charging current. To achieve the desired performance for wireless applications, the Wu active inductor, which is a low‐noise and high‐quality factor active inductor, is employed in each delay cell for the first time. Using an active inductor provides a wide tuning range and also allows for proper phase noise and low power consumption. The proposed circuit is designed and simulated using standard 180‐nm CMOS technology with a 1.8‐V voltage source (VDD). The circuit is designed to achieve a tuning range of 2.15 GHz with a center frequency oscillation of 2.745 GHz, over the control voltage variation of 1.4 V (0 to 1.4 V). To achieve the desired performance, the circuit consumes an average power of 1.99 mW. It achieves a phase noise of − 91.2 dBc/Hz at 1 MHz offset frequency, indicating effective noise suppression. The figure of merit (FoM) for the circuit is − 156.9 dBc/Hz, representing its overall performance. The final layout of the circuit estimates an area of 0.00072 mm2. Various analyses, including Monte–Carlo simulations, PVT (process, voltage, temperature) variation analysis, and other relevant analyses, have been performed to ensure the reliable performance of the proposed circuit.

Effects of acute hippocampal stimulation in the nonhuman primate penicillin model of temporal lobe seizures

Asynchronous distributed multielectrode stimulation (ADMES) is a novel approach to deep brain stimulation for medication resistant temporal lobe epilepsy that has shown promise in rodent and in vitro seizure models. To further evaluate its effects on a pre-clinical model, we characterized the effect of unilateral ADMES in an NHP model of temporal lobe seizures induced by intra-hippocampal injection of penicillin (PCN). Four non-human primates were used for this study in two contemporaneous cohorts. One cohort (n = 3 hemispheres) was implanted with the Medtronic RC + S stimulation (GIN cohort) and recording system connected to two 4-contact ring electrodes to evaluate three unilateral stimulation patterns: 7 Hz Ring ADMES, 20 Hz Dual Ring, and 125 Hz Dual Ring (analog of clinical stimulation). In an additional cohort (EPC cohort, n = 2), two 12-contact segmented electrodes were implanted in the right hippocampus and connected to an externalized recording and stimulation system to allow more flexibility in the stimulation pattern. In this second cohort, 4 variations of stimulation were evaluated (7 Hz Full ADMES, 7 Hz Ring ADMES, 31 Hz Wide Ring, and 31 Hz Dual Ring). In the GIN cohort, we found an increase in seizure frequency and time spent in seizure during the 7 Hz Ring ADMES stimulation compared to the respective post-stimulation. A similar post-stimulation effect was found in the EPC cohort. We also found an increase in seizure frequency during the 7Hz full ADMES compared to the respective post-stimulation. However, we did not find a difference between pre-stimulation and stimulation conditions suggesting a possible post stimulation effect of the 7Hz hippocampal stimulation. In conclusion, in the NHP PCN model of temporal lobe seizures, acute asynchronous hippocampal stimulation was not therapeutic, however, our findings related to the post-stimulation effect can support future studies using hippocampal stimulation for the treatment of temporal lobe epilepsy.

Design of a Low Power and Wide Frequency Range Ring VCO

This paper proposes a low-power, wide frequency range ring VCO circuit structure based on the application of switch power supply modulation technology. Circuit simulation is completed based on CMSC 180nm BCD process. Simulation results show that the VCO circuit can work at a power supply voltage of 3-5 V, and it has a relatively wide frequency range. Under a 5V power supply voltage and a control voltage range of 4.2V~2.2V, the output frequency range is 0.4-11.96MHz. Under a 3V power supply voltage and a control voltage of 2.2V~0.2V, the output frequency range is 0.56~12.3MHz. For the VCO circuit, the maximum average working current of the power supply is less than 80uA, and the average current of the power supply is only 35uA under a 5V power supply voltage and a 10MHz working frequency. The VCO circuit meets the requirements of low power consumption. It can be applied to switch power supply chips.

Enhancing Load-Bearing Capacity of Calcareous Sands through Gel Stabilization: A Mechanical and Material Characterization Study.

Calcareous sands often display wide ring grain configurations, high intragranular porosity, a complex structure, and low grain hardness. These attributes typically do not meet the strength criteria necessary to sustain overlying infrastructure in civil engineering applications. This study investigates gel stabilization techniques, blending gel material with calcareous sand at concentrations ranging from 5% to 22%, followed by curing periods of 3 to 28 days to evaluate the load-bearing capacity. Subsequently, an unconfined compressive test is performed to determine the gel material content in stabilized specimens and investigate the influence of gel material types. The gel material-to-sand ratios employed are set at 5%, 10%, and 16% for Portland cement and 13%, 16%, and 22% for gypsum. After that, a triaxial consolidated undrained test is conducted to assess mechanical behavior, pore water pressure, and mechanical properties. The findings reveal increased dilation, stress-strain hardening, and softening post-yield, regardless of gel material type. Principal stress ratios, secant modulus, and cohesion show a positive correlation with maintenance duration and binder content, with implications for improved load-bearing capacity. The study also elucidates the qualitative relationship between secant modulus E50 and confining pressure.

The Use of PVD Coatings for Anti-Wear Protection of the Press-In Connection Elements

Press-in connections are the commonly used methods for connecting machinery components. In relation to that wide use, those connections are liable to various types of damage and wear. Therefore, this article proposes one of the methods which may improve the life of the press-in connection. CrN+OX, TiN, and ZrN coatings made in the PVD technology were used. The coatings were applied on shafts mating with sleeves and subjected to a rotational bending moment. Tests and observations were conducted that enabled the assessment of the influence of those coatings on the development of wear, in particular fretting, in the tribological kinematic pair. The tests showed the development of wear on all the observed shaft surfaces, with a lower intensity of damage recorded on coated shafts compared to uncoated ones. The traces of fretting wear were noted each time at the edges of the connection, which is due to the mechanism of wear development under rotational bending conditions. In the case of uncoated shafts, wear occurs at the entire circumference of the axle seat in the form of a 3–4 mm wide ring; however, that width is different on either side. In the case of coated shafts, wear occurs on the circumference of the axle seat in the form of a thin ring 1–3 mm wide, depending on the coating. In the case of a ZrN coating, fretting wear appears locally. The largest surface area occupied by wear is 0.75 cm2. Fretting wear comprises mainly material build-ups, pits, and surface scratches. In addition to fretting wear, damage resulting from the process of forcing the sleeve onto the shaft was observed on the tested surfaces.

Preferential vasodilator effects of levosimendan in resistance pulmonary arteries in a rodent pulmonary embolism model

Background: We compared the vasoactive effects of levosimendan on isolated conduit pulmonary arteries (CPA) and resistance pulmonary arteries (RPA) versus mesenteric arteries, and we assessed the pulmonary artery (PA) vascular function and the PA vasoactive effects of levosimendan in a rodent pulmonary embolism (PE) model.Methods: One group of male Wistar rats (200-300 g) was killed by decapitation to obtain pulmonary and mesenteric rings. Another group was assigned to a massive PE or saline solution infusion. After euthanasia, mesenteric arteries and CPA (1-2 mm) and RPA (≤0.5 mm) were dissected and cut into 2-3 mm wide rings, recording contractile tension. We obtained the concentration-response curves of cumulative doses of levosimendan on pre-contracted arterial rings from decapitated and sham/embolized animals. A set of RPA rings was exposed to acute hypoxia. The effect of PE on the pulmonary vasoactive function was assessed by dose-response curves of acetylcholine (ACh) and endothelin-1 (ET-1) of PA rings from sham/embolized animals.Results: Levosimendan relaxant potency of RPA was similar to that of mesenteric arteries and higher than CPA, while mesenteric rings showed the maximal relaxant effect, followed by RPA and CPA, respectively. PE did not affect the vasoactive response of PA rings either to ACh or to ET-1, and the relaxant effects of CPA and RPA to levosimendan were also preserved. Acute hypoxia reduced (p&lt;0.05) but did not avoid the RPA relaxant effect of levosimendan.Conclusions: Levosimendan is a more specific vasodilator of RPA with a similar relaxant potency as mesenteric arteries, which is preserved after PE but significantly reduced during hypoxia.

A Comparison of the Chronologies of Introduced versus Native Coniferous Tree Species Growing in Northwestern Poland during the Period of Global Warming

The ongoing climatic changes are causing the extinction of numerous species or their withdrawal from previously occupied areas. The environmental and economic significance of introduced species may increase. The aim of the present study was to examine the rate of growth of coniferous species growing in northwestern Poland and to analyze the tree ring width–climate relationships. Six tree species were selected for this study. Two of these species have natural occurrences in Poland: Pinus sylvestris and Larix decidua. The remaining four species were introduced from North America: Chamaecyparis lawsoniana, Thuja plicata, Pseudotsuga menziesii, and Pinus strobus. Samples were collected from 131 trees using a Pressler borer at 1.3 m above ground. Tree ring widths were measured down to 0.01 mm. Climatic data were retrieved from a weather station located 23 km from the study plot. The average tree ring width reaches the lowest value for the P. sylvestris chronology (1.62 mm/year) and for P. strobus (1.69 mm/year), and the highest value is reached for T. plicata (2.80 mm/year) and P. menziesii (2.56 mm/year). The analysis of weather conditions in the designated pointer years and the response function analysis indicate that winter and early spring air temperature is the factor responsible for the formation of wide tree rings in the following species studied: P. sylvestris, C. lawsoniana, P. menziesii, and T. plicata. For L. decidua and P. strobus, the climate–growth relationships are different: weather conditions in the previous growth year are important, and it is the weather in the late spring and summer months. Two of the investigated introduced species (T. plicata and P. menziesii) are characterized by very good acclimatization and are best adapted to the new habitat during the current climate changes. These tree species can constitute a basis for replacing native species, which, due to increasingly severe droughts and higher temperatures, are doing less and less well in their current habitats. Foresters wanting to conduct sustainable forest management will look for replacement species that are well adapted to new habitat conditions in order to maintain the continuity of forest cover.

Spruce Protection against Ips typographus with Anti-Attractant Blend of Tree-Based Semiochemicals: From Small Experimental Plots to Stand Scales

Tree-killing bark beetles require rapid management, such as anti-attractants, to stop the enlargement of attack hot-spots. We tested two newer anti-attractant blends, both without verbenone and one with the addition of trans-4-thujanol, in traps against standard pheromone baits for inhibition of catch. Both blends provided effective catch reduction (&gt;95%). We also tested these anti-attractant blends in tree protection experiments for two years. We had experimental plots with a center of an anti-attractant protected tree zone, with no traditional control area, but we followed tree kills in 10 m wide concentric rings to 100 m. In 2020, we had 12 plots, and 9 plots in 2021. Monitoring by low-strength pheromone traps followed beetle flight averaging 300/trap during the shorter period, August 2020, and 5000/trap during the longer period, May to August 2021. The blends of anti-attractants were 100% effective in avoiding tree mortality in both treated trees and their surroundings. There were no bark beetle attacks on any treated trees, and there was zero tree mortality up to 19 m in 2020, and up to 30 m in 2021, thus full protection to circa 20 m. The density of killed trees then increased from close to zero, over 20 to 50 m, reaching a level of ca 30 (trees/ha) then declined. The spatial pattern of tree mortality on our experimental plots was highly heterogeneous and individual 10m-ring data points on tree kill density could not be statistically separated. In contrast, a non-linear regression model showed a continuous increase of attacks over the distance from the center to a peak ca 60m, followed by a decline. This model agrees partly with the only similar study in the literature, but importantly, it does not give a peak of kill density at distances between the first and second rings close to treatment zones. Such patterns of close-quarter kills have been observed as a “switching” of attack in this and other scolytid systems manipulated by anti-attractants, but not in the present study, likely due to the elimination of verbenone from our blends.

IRGWP: An international network of key players for a better understanding and industrial developments in wood protection and preservation

IRGWP: An international network of key players for a better understanding and industrial developments in wood protection and preservation

Linking Topographical Ring Features to Geochemical and Geophysical Anomalies

Circular features in forests seen from air have been studied for several decades at different locations around the world. Forest rings, as they are called in Canada’s boreal forests, express several geochemical (pH, carbonate content) and geophysical (surface potential) anomalies on their 20–30 m wide ring edges. Although it has been proposed that microbial processes may cause these anomalies, the exact mechanisms of ring formation are still unknown. We focused on the Thorn North forest ring in Ontario, Canada to correlate the surface potential anomaly to soil gas concentrations. Field measurements showed that the surface potential drop at the ring edge center is framed by peaks in CO2 production, which is linked to O2 depletion and methane generation. Carbon isotope signatures were found to drop to lighter values (down to −20‰), suggesting increased respiration. Higher concentrations of uronic acids bound to extracellular polymeric substances were found, indicating that the surface potential anomaly is linked to respiration. 16S rRNA gene sequencing of shallow soil did not indicate a dominant microbial group on the edges; instead, principal component analysis showed that the microbial composition was controlled by the substrate (clayey vs. sandy soil), therefore future studies should focus on deeper ground layers.

Dendrochronological Analysis of One-Seeded and Intermediate Hawthorn Response to Climate in Poland

Although the hawthorn is not a forest-forming species, and it has no high economic significance, it is a very valuable component of forests, mid-field woodlots or roadside avenues. The literature, however, lacks information on the growth rate, growth phases, or growth–climate–habitat relationship for trees of this genus. This work aimed to establish the rate of growth of Craraegus monogyna and C. xmedia Bechst growing in various parts of Poland, in various habitats; analyze the growth–climate relationship; and distinguish dendrochronological regions for these species. Samples were taken using a Pressler borer from nine populations growing in different parts of Poland, from a total of 192 trees (359 samples). The tree-ring width was measured down to 0.01 mm. The average tree-ring width in the studied hawthorn populations ranged from 1.42 to 3.25 mm/year. Using well-established cross-dating methods, nine local chronologies were compiled with tree ages between 45 and 72 years. Dendroclimatic analyses (pointer year analysis, correlation and response function analysis) were performed for a 33-year period from 1988 to 2020, for which all local chronologies displayed EPS &gt; 0.85. The tree-ring width in the hawthorn populations depended mostly on temperature and rainfall through the May–August period. High rainfall and the lack of heat waves through these months cause an increase in cambial activity and the formation of wide tree rings. Conversely, rainfall shortages through this period, in conjunction with high air temperatures, caused growth depressions. Cluster analysis enabled the identification of two dendrochronological regions among the hawthorn in Poland: a western and eastern region, and a single site (CI), whose separation was most likely caused by contrasting habitat and genetic conditions. The obtained results highlight the need for further study of these species in Poland and other countries.