In this paper, we proposed a method for THz sub-comb generation based on spectral interference. The result of our calculation indicated that the THz pulse train, generated by surface-emitted optical rectification of femtosecond (fs) laser pulse in periodically poled lithium niobate (PPLN), has a comb-like spectrum. The characteristic of this THz sub-comb was analyzed both in frequency and time domain. Compared with the THz frequency comb emitted by a photoconductive antenna (PCA), THz sub-comb has a lower spectral resolution and wider free spectral range. Thus it could be an ideal source for wavelength division multiplexing (WDM) in THz wireless communication system.