Sustainable and efficient refrigerants are essential due to increasing regulatory constraints on traditional high-GWP refrigerants. This study investigates the potential of natural refrigerants, specifically air, in Reverse Brayton Cycles (RBC) for low-temperature applications. Unlike carbon dioxide and ammonia, which pose limitations and safety concerns below -40°C, air offers a safer and more versatile solution due to its excellent thermodynamic properties and availability. An experimental RBC was constructed using automotive components like centrifugal compressors, a radial turbine, and inter-coolers. These cost-effective and accessible components shift the refrigeration paradigm. The RBC was tested to optimize the Coefficient of Performance (COP) at -100°C while dissipating 2 kW, a typical scenario for whole-body cryotherapy (WBC). Key control parameters included the pressure ratio of the centrifugal compressors and the position of the stator vanes in the variable geometry turbine (VGT). The optimization process resulted in a COP increase of up to 28%. Additionally, a 1D gas-dynamic model validated these results, suggesting that different component selections could enhance performance by 17% compared to the experimental optimum point. Air-based RBC systems using automotive components can effectively achieve temperatures below -40°C, offering a viable, eco-friendly alternative to traditional refrigerants. This advancement addresses regulatory challenges and contributes to the scientific community by providing a sustainable refrigeration solution using commercially available components and demonstrating improvements through experimental data.