Minor grapevine varieties represent enormous value in terms of cul?tural heritage, diversification of products with higher economic value and adaptation to environmental conditions. This paper presents the results of research on certain production and technological characteristics (mechanical composition of clusters and berries, as well as chemical composition of grape must) of a local, i.e. minor white wine variety Sremska Zelenika examined in the agro-ecological conditions of the Fruska Gora wine-growing sub-region (Srem wine-growing region) in the locality of Sremski Karlovci. In the two-year research (2020 and 2021), without any yield reduction, it was determined that the average mass of the cluster was 266.5 g. The average sugar content in the grape must of the tested variety was 19.8%, the average content of total acids was 6.75 g/l and the average pH of the grape must was 3.27. Comparing the results of previous researches conducted in the same locality, it is concluded that in this research the minor variety of Sremska Zelenika has some better production and technological characteristics.