White Fiber Research Articles

The role of wet wipes and sanitary towels as a source of white microplastic fibres in the marine environment

Understanding source elements of the ocean plastic crisis is key to effective pollution reduction management and policy. The ubiquity of microplastic (MP) fibres in the oceans is considered to derive primarily from clothing fibres released in grey water. Microplastic fibres degraded from widely flushed personal care textile products (wet wipes and sanitary towels) have not been clearly identified in aquatic systems to date. Unregulated personal hygiene and sanitary product labelling fails to identify textile materials. This study demonstrated that white MP fibres in sediments adjacent to a wastewater treatment plant (WWTP) are comparable with white fibres from sewage-related waste and commercially available consumer sanitary products. Commercially available non-flushable wipes are manufactured from either polyethylene terephthalate (PET), polypropylene (PP), or a combination of PET and cellulose. Fifty percent of brands labelled flushable that were tested were comprised of a mixture of PET and cellulose and the remainder of cellulose alone. Sanitary towels are made from PP, PE, or a combination of high-density polyethylene (HDPE) and PP. The accumulation of large quantities of washed-up sewage-related macro-debris (including wet wipes and sanitary towels) intermingled with seaweed biomass adjacent to the WWTP was associated with a combined sewer overflow. Microplastic fibres extracted from this waste were similar to those extracted from intertidal sediments in close proximity to the WWTP over a ten-month period. In comparison, fibres extracted from locations spatially removed from the WWTP were primarily comprised of ABS, PP and polystyrene. The results confirm that wet wipes and sanitary towels flushed down toilets are an underestimated source of white MP fibres in the environment. Given the global distribution and projected growth of the non-woven textile industry, there is a need for increased public awareness of MP pollution in the marine environment from the inappropriate disposal of sanitary products down the toilet, instead of diversion to alternative land-based waste management.

Quality “crispy enbal”, fortified with seaweed

Enbal is the local name of cassava which is popular in the Kei Islands. The purpose of this research is to improve the competitiveness of crispy “Enbal” made from 2 types of enbal (Manihot utilisima) enriched with seaweed (Eucheuma cottonii) to increase fiber content. This research is conducted in two steps. The first stage is the production of “enbal” flour from two types of enbal namely white and yellow enbal then analyzed with antioxidants (beta carotene) and fiber. The second stage is the production of crispy seaweed by adding 10, 20 and 30% seaweed. Then analyzed water content, protein, fat, fiber and beta-carotene. The results of the first phase of the study showed that the white fibers (2.02%) and yellow (2.47%) were not significantly different, while the beta-carotene content of yellow enbal (4.27mg / kg) was higher than the white (<0.02mg / kg). The results of the second stage of the study showed that the yield of crispy seaweed Enbal enriched with 30% seaweed was higher than the other treatments, while the other parameters did not differ.

Characteristics of Recycled Polypropylene Fibers as an Addition to Concrete Fabrication Based on Portland Cement

High-performance concrete has low tensile strength and brittle failure. In order to improve these properties of unreinforced concrete, the effects of adding recycled polypropylene fibers on the mechanical properties of concrete were investigated. The polypropylene fibers used were made from recycled plastic packaging for environmental reasons (long degradation time). The compressive, flexural and split tensile strengths after 1, 7, 14 and 28 days were tested. Moreover, the initial and final binding times were determined. This experimental work has included three different contents (0.5, 1.0 and 1.5 wt.% of cement) for two types of recycled polypropylene fibers. The addition of fibers improves the properties of concrete. The highest values of mechanical properties were obtained for concrete with 1.0% of polypropylene fibers for each type of fiber. The obtained effect of an increase in mechanical properties with the addition of recycled fibers compared to unreinforced concrete is unexpected and unparalleled for polypropylene fiber-reinforced concrete (69.7% and 39.4% increase in compressive strength for green polypropylene fiber (PPG) and white polypropylene fiber (PPW) respectively, 276.0% and 162.4% increase in flexural strength for PPG and PPW respectively, and 269.4% and 254.2% increase in split tensile strength for PPG and PPW respectively).

An Untargeted Metabolomics Investigation of Jiulong Yak (Bos grunniens) Meat by 1H-NMR.

Yak represents the main meat source for Tibetan people. This work aimed to investigate the metabolome of raw meat from Jiulong yaks, focusing on specimens farmed and harvested locally through traditional procedures. Untargeted nuclear magnetic resonance spectroscopy (1H-NMR) was selected as the analytical platform. Samples from longissimus thoracis, trapezius, triceps brachii and biceps femoris muscles, with different prevalences of red and white fibers, were selected. Among the fifty-three metabolites quantified in each of them, carnitine, carnosine, creatine and taurine are known for their bioactive properties. Twelve molecules were found to be differently concentrated in relation to muscle type. Longissimus thoracis, compared to biceps femoris, had higher concentrations of carnosine and formate and lower concentrations of mannose, inosine, threonine, IMP, alanine, valine, isoleucine, tyrosine, phenylalanine and leucine. A metabolic pathway analysis suggested that the main pathways differing among the muscles were connected to the turnover of amino acids. These results contribute to a deeper understanding of yak raw meat metabolism and muscle type differences, which can be used as an initial reference for the meat industry to set up muscle-specific investigations. The possibility of simultaneously quantifying several bioactive compounds suggests that these investigations could revolve around meat’s nutritional value.

White light emission PMMA fibre co-doped with 1,4-Bis(2-methylstyryl) benzene and Rhodamine B for new optical applications

The article presents the white light emission poly (methyl methacrylate) cylindrical fibre fabricated by co-doping by organic luminescent dyes. The used composition of aromatic hydrocarbon 1,4-Bis(2-methylstyryl) benzene Bis(MSB) and xanthene dye Rhodamine B (RhB) assures covering the emission in the range 370–700 nm under 355 nm excitation (Nd-YAG, third harmonic). The chosen complexes, according to the wide emission bands in the visible spectrum range and relatively large spectral shift of emission Bis(MSB) and absorption bands of RhB complexes, reduce the spectrum shape modification in the polymeric fibre structure and can be effectively used for lightning applications. The fabrication process and characterization of luminescent properties (emission spectrum, chromaticity CIE 1931 coordinates) vs. fibre length (0.5 m) are presented and discussed.

A case study of a micro-inversion event in dark brown fibre cotton (Gossypium hirsutum)

Structural variation is a major type of genetic variation that can potentially induce powerful genetic effects. In this study, we examined the Inv(A07)p1.09p2.23 genetic inversion in brown fibre cotton at the individual and population genetics levels. A dark-brown fibre mutant that resulted from a distant hybridization between Gossypium barbadense and G. hirsutum, and a natural population including 30 dark-brown, 70 light-brown and 21 white fibre cotton accessions were collected to perform a functional study of this micro-inversion. The results showed that Inv(A07)p1.09p2.23 can be detected by high-throughput resequencing method, and induce micro-deletion, gene disruption (Ghir_A07G000980) and abnormal gene expression in the breakpoint regions. Inv(A07)p1.09p2.23 existed in only dark-brown fibre cotton, had undergone negative selection in elite brown fibre cultivars, and was significantly associated with fibre colour and nine fibre traits. In the Inv(A07)p1.09p2.23 region, nucleotide diversity was lower, recombination was absent, and linkage disequilibrium was higher. Overall, this inversion event in dark-brown fibre cotton produced significant genetic effects, and this study will guide us to better understand the genetic effects of inversion events in dark-brown fibre cotton.

Application of direct electrical stimulation in awake craniotomy for glioma resection in the motor area

Objective To explore the effect of direct electrical stimulation in awake craniotomy for glioma resection in the motor area. Methods We conducted a retrospective analysis of clinical data of 34 patients with gliomas in the motor area who were admitted to Department of Neurosurgery, General Hospital of Southern Theatre Command from March 2015 to July 2017. The tumor was located in the left hemisphere in 16 patients and right hemisphere in 18. The gliomas were in supplementary motor area or premotor cortex in 23 cases, the central area in 9 cases, and supplementary motor area or premotor cortex invading the central area in 2 cases. All patients underwent awake craniotomy under general anesthesia. Neuronavigation and/or intraoperative ultrasound were employed to locate the lesion. Direct electrical stimulation was used for cortical and subcortical mapping of the important eloquent areas. The tumors were removed according to the functional boundary.Neural function and the degree of tumor resection were evaluated after operation. Results Of the 34 patients, 24 had a motor response after direct cortical electrical stimulation, 13 had abnormal sensations, and 10 revealed language-related cortices through mapping. For subcortical electrical stimulation, there were 24 cases of motor response, 1 case of abnormal sensation, and 8 cases of language disorders. A total of 30 cases (88.2%) of tumor removal reached functional boundaries, and subcortical electrical stimulation did not identify functional fiber in the remaining 4 (11.8%) cases which were all high-grade gliomas. Within 48 hours post surgery, the head MRI indicated total resection of tumor in 22 cases (64.7%), subtotal resection in 9 (26.5%), and partial resection in 3 (8.8%). The follow-up time of 34 patients was (23.6 ± 8.6) months (11.3-39.3)months.There were 29 cases (85.3%) which showed early postoperative neurofunctional disorders or worsening of pre-existing neurological deficits. Three cases (8.8%) developed late postoperative neurological dysfunction worse than preoperative conditions, of which 1 case was mild, 1 case was moderate and 1 case (2.9%) was severe. Of the 16 patients with preoperative neurological dysfunction or increased intracranial pressure, 13 had improved neurological function in 3 months after surgery, 2 were maintained in preoperative state and 1 had severe neurological deficits. Conclusions Functional mapping through direct electrical stimulation and continuous monitoring of the cortical and subcortical white fibers in the motor area during awake craniotomy could maximize the safe resection of glioma in the motor area, the incidence of long-term severe neurological deficits is low, and the quality of life could be improved after surgery. Key words: Glioma; Motor area; Supplementary motor area; Pyramid tract; Direct electrical stimulation

Functional correlations of axial muscle fiber type proportions in the waterfall-climbing Hawaiian stream fish Sicyopterus stimpsoni.

Assessing the factors that contribute to successful locomotor performance can provide critical insight into how animals survive in challenging habitats. Locomotion is powered by muscles, so that differences in the relative proportions of red (slow-oxidative) vs. white (fast-glycolytic) fibers can have significant implications for locomotor performance. We compared the relative proportions of axial red muscle fibers between groups of juveniles of the amphidromous gobiid fish, Sicyopterus stimpsoni, from the Hawaiian Islands. Juveniles of this species migrate from the ocean into freshwater streams, navigating through a gauntlet of predators that require rapid escape responses, before reaching waterfalls which must be climbed (using a slow, inching behavior) to reach adult breeding habitats. We found that fish from Kaua'i have a smaller proportion of red fibers in their tail muscles than fish from Hawai'i, matching expectations based on the longer pre-waterfall stream reaches of Kaua'i that could increase exposure to predators, making reduction of red muscle and increases in white muscle advantageous. However, no difference in red muscle proportions was identified between fish that were either successful or unsuccessful in scaling model waterfalls during laboratory climbing trials, suggesting that proportions of red muscle are near a localized fitness peak among Hawaiian individuals.

Microstructural correlates of 23Na relaxation in human brain at 7 Tesla

23Na provides the second strongest MR-observable signal in biological tissue and exhibits bi-exponential T2∗ relaxation in micro-environments such as the brain. There is significant interest in developing 23Na biomarkers for neurological diseases that are associated with sodium channel dysfunction such as multiple sclerosis and epilepsy. We have previously reported methods for acquisition of multi-echo sodium MRI and continuous distribution modelling of sodium relaxation properties as surrogate markers of brain microstructure. This study aimed to compare 23Na T2∗ relaxation times to more established measures of tissue microstructure derived from advanced diffusion MRI at 7 ​T. Six healthy volunteers were scanned using a 3D multi-echo radial ultra-short TE sequence using a dual-tuned 1H/23Na birdcage coil, and a high-resolution multi-shell, high angular resolution diffusion imaging sequence using a 32-channel 1H receive coil. 23Na T2∗ relaxation parameters [mean T2∗ (T2∗mean) and fast relaxation fraction (T2∗ff)] were calculated from a voxel-wise continuous gamma distribution signal model. White matter (restricted anisotropic diffusion) and grey matter (restricted isotropic diffusion) density were calculated from multi-shell multi-tissue constrained spherical deconvolution. Sodium parameters were compared with white and grey matter diffusion properties. Sodium T2∗mean and T2∗ff showed little variation across a range of white matter axonal fibre and grey matter densities. We conclude that sodium T2∗ relaxation parameters are not greatly influenced by relative differences in intra- and extracellular partial volumes. We suggest that care be taken when interpreting sodium relaxation changes in terms of tissue microstructure in healthy tissue.

Caracterización física y mecánica de compuestos de Guazuma crinita Mart. a base de polipropileno virgen

Se elaboraron materiales compuestos plástico madera con base en partículas de madera de la especie Guazuma crinita (bolaina blanca), proveniente del raleo de 4, 5 y 6 años, con polipropileno virgen (PP), además se utilizó como agente acoplante anhídrido maleíco de polipropileno (MAPP). La producción de probetas se hizo por el método de extrusión, compresión térmica y corte por láser. Las partículas de bolaina blanca se tamizaron con tamaños de malla ASTM: -40/+60, -60/+80 y -80/+100. Las proporciones de mezcla polipropileno/bolaina fueron: 70/30, 80/20 y 90/10. Todas las formulaciones incluyeron 2 % de MAPP como agente acoplante. Se evaluaron las propiedades físicas de contenido de humedad, densidad, absorción e hinchamiento; así como, las propiedades mecánicas de flexión estática, tensión y resistencia al impacto. Para la discusión de resultados se realizó una caracterización anatómica de la fibra (longitud, diámetro, espesor de pared, diámetro del lumen y coeficiente de esbeltez), además de un análisis químico de los componentes de la misma (extractivos, holocelulosa, lignina y cenizas). Los resultados permiten apreciar influencia de la variable proporción de mezcla sobre las principales propiedades físicas, así como de la variable tamaño de partícula respecto a la tensión. La edad de la madera no representó una fuente de variabilidad significativa.

Variable impacts of L-arginine or L-NAME during early life on molecular and cellular markers of muscle growth mechanisms in rainbow trout

Two experiments were conducted to test if manipulations of the Arginine-Nitric oxide pathway during the early life of rainbow trout would act on its early myogenic process. In experiment 1, trout embryos were immersed at 72° days post-fertilization (°dpf) or 104°dpf in water alone (control treatment, C) or containing 2 mM/L L-Arg (treatment A) or 1 mM/L of L-NAME, a NOS inhibitor (treatment N). We observed the beginning of expression of myf5 and fmhc genes at 72°dpf and 96°dpf, respectively. “A” treatment doubled the free Arg content of eggs but did not affect either the pattern of expression of myf5 and fmhc, nor white muscle cross-sectional area and number of white muscle fibres at hatching, nor embryo survival and fry growth. “N” treatment also did not affect these markers. In experiment 2, trout fry were fed from first feeding onwards and during 20 days either a control diet (C) or the same diet supplemented with L-NAME (0.1 g/100 g diet, N-diet). In C-fed fry, distribution of a single meal after overnight fasting induced changes in pcna, myod1, myog, fmhc, inos, nnos and ctsd gene expressions. N-feeding decreased fry growth but did not change their growth trajectory or survival. Twenty days of N-feeding led, compared to C-feeding, to changes in kinetics of transcription of pcna, myod1, myog, fmhc, inos, nnos, ctsd genes and to decreased white muscle cross-sectional area, total number of white muscle fibres, and number of large muscle fibres. L-NAME feeding thus decreased fry muscle growth by altering both hyperplasia and hypertrophy.

Early Exposure to THC Alters M-Cell Development in Zebrafish Embryos.

Cannabis is one of the most commonly used illicit recreational drugs that is often taken for medicinal purposes. The psychoactive ingredient in cannabis is Δ9-Tetrahydrocannabinol (Δ9-THC, hereafter referred to as THC), which is an agonist at the endocannabinoid receptors CB1R and CB2R. Here, we exposed zebrafish embryos to THC during the gastrulation phase to determine the long-term effects during development. We specifically focused on reticulospinal neurons known as the Mauthner cells (M-cell) that are involved in escape response movements. The M- cells are born during gastrulation, thus allowing us to examine neuronal morphology of neurons born during the time of exposure. After the exposure, embryos were allowed to develop normally and were examined at two days post-fertilization for M-cell morphology and escape responses. THC treated embryos exhibited subtle alterations in M-cell axon diameter and small changes in escape response dynamics to touch. Because escape responses were altered, we also examined muscle fiber development. The fluorescent labelling of red and white muscle fibers showed that while muscles were largely intact, the fibers were slightly disorganized with subtle but significant changes in the pattern of expression of nicotinic acetylcholine receptors. However, there were no overt changes in the expression of nicotinic receptor subunit mRNA ascertained by qPCR. Embryos were allowed to further develop until 5 dpf, when they were examined for overall levels of movement. Animals exposed to THC during gastrulation exhibited reduced activity compared with vehicle controls. Together, these findings indicate that zebrafish exposed to THC during the gastrula phase exhibit small changes in neuronal and muscle morphology that may impact behavior and locomotion.

Thermal properties and surface chemistry of cotton varieties mineralized with calcium carbonate polymorphs by cyclic dipping.

In this study, hydroentangled cotton nonwovens were identified as effective hosts for mineralization of calcium carbonate (CaCO3) polymorphs to modify and improve their properties. All cotton varieties studied, including raw white cotton, scoured white cotton, and raw brown cotton, readily crystallized CaCO3via a simple cyclic dipping process. A combination of analyses agreed that the surface chemistry of cotton fibers influenced the formation of different CaCO3 polymorphs. Scoured white cotton that consisted of almost pure cellulose predominantly produced the most stable calcite, whereas raw white and raw brown cottons that contain proteins facilitated the production of partial metastable vaterite. The morphology of calcite was better defined on the scoured cotton. The mineralization altered the hydrophobic surface of raw cottons to be hydrophilic, i.e., two-fold increase in moisture regain and decrease in water contact angle from 130 to 0 degrees. The mineralized cottons also exhibited improved thermal resistance, i.e., slower thermal decomposition with decreased activation energies and reduction in heat release capacity by up to 40%.

Photon arrival time tagging with many channels, sub-nanosecond deadtime, very high throughput, and fiber optic remote synchronization.

Time-Correlated Single Photon Counting (TCSPC) and time tagging of individual photon detections are powerful tools in many quantum optical experiments and other areas of applied physics. Using TCSPC, e.g., for the purpose of fluorescence lifetime measurements, is often limited in speed due to dead-time losses and pileup. We show that this limitation can be lifted by reducing the dead-time of the timing electronics to the absolute minimum imposed by the speed of the detector signals while maintaining high temporal resolution. A complementing approach to speedy data acquisition is parallelization by means of simultaneous readout of many detector channels. This puts high demands on the data throughput of the TCSPC system, especially in time tagging of individual photon arrivals. Here, we present a new design approach, supporting up to 16 input channels, an extremely short dead-time of 650 ps, very high time tagging throughput, and a timing resolution of 80 ps. In order to facilitate remote synchronization of multiple such instruments with highest precision, the new TCSPC electronics provide an interface for White Rabbit fiber optic networks. Beside fundamental research in the field of astronomy, such remote synchronization tasks arise routinely in quantum communication networks with node to node distances on the order of tens of kilometers. In addition to showing design features and benchmark results of new TCSPC electronics, we present application results from spectrally resolved and high-speed fluorescence lifetime imaging in medical research. We furthermore show how pulse-pileup occurring in the detector signals at high photon flux can be corrected for and how this data acquisition scheme performs in terms of accuracy and efficiency.

Continuous dry–wet spinning of white, stretchable, and conductive fibers of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and ATO@TiO2 nanoparticles for wearable e-textiles

Stretchable electronic textiles are urgently called for due to the emergence of next generation wearable electronics that can undergo various mechanical deformations that exist almost everywhere in our daily lives.

Effects of shading material on the growth, yield and quality of green pepper (Capsicum annum L.) grown under hot and humid climatic conditions

Green pepper (Capsicum annum L.) is one of the most popular vegetables in the Kingdom of Eswatini, with its production mainly under irrigation in open fields. Shade nets are used to modify the crop micro-environment to improve plant growth and yield. This experiment was conducted at Mphaphati, under Eswatini Water and Agricultural Development Enterprises, (ESWADE) in the summer of January and April 2018. The objective of this study was to determine the effect of plastic and net covered tunnels on the growth, yield and quality of green pepper (Capsicum annum var. Crusader L.). Shading materials were used white fiber plastic tunnel, green net tunnel (40%) and black net tunnel (40%). The treatments were arranged in Randomized Complete Block Design (RCBD) with three replications. The results revealed that plants grown under the green net covered tunnel significantly (P

Evolution of the sheep coat: the impact of domestication on its structure and development.

Wild sheep and many primitive domesticated breeds have two coats: coarse hairs covering shorter, finer fibres. Both are shed annually. Exploitation of wool for apparel in the Bronze Age encouraged breeding for denser fleeces and continuously growing white fibres. The Merino is regarded as the culmination of this process. Archaeological discoveries, ancient images and parchment records portray this as an evolutionary progression, spanning millennia. However, examination of the fleeces from feral, two-coated and woolled sheep has revealed a ready facility of the follicle population to change from shedding to continuous growth and to revert from domesticated to primitive states. Modifications to coat structure, colour and composition have occurred in timeframes and to sheep population sizes that exclude the likelihood of variations arising from mutations and natural selection. The features are characteristic of the domestication phenotype: an assemblage of developmental, physiological, skeletal and hormonal modifications common to a wide variety of species under human control. The phenotypic similarities appeared to result from an accumulation of cryptic genetic changes early during vertebrate evolution. Because they did not affect fitness in the wild, the mutations were protected from adverse selection, becoming apparent only after exposure to a domestic environment. The neural crest, a transient embryonic cell population unique to vertebrates, has been implicated in the manifestations of the domesticated phenotype. This hypothesis is discussed with reference to the development of the wool follicle population and the particular roles of Notch pathway genes, culminating in the specific cell interactions that typify follicle initiation.

Developing of Indonesian colored cotton varieties to support sustainable traditional woven fabric industry

There are four color groups of cotton fiber, namely white, brown, green and blue. The most cultivated colored-cottons are brown and green cotton fiber. The program for assembling colored-cotton varieties, especially brown color in Indonesia began in 2006. Crossing activities used three Indonesian National Cotton superior varieties, namely Kanesia 7, Kanesia 8 and Kanesia 9 as female founder and three cotton accessions with brown fiber cotton characters 73814, Multiple Dominant and RLBL as male founder. From crosses followed by selection based on desired character, tests of selected lines and multi-location tests of expected lines, we obtained 3 brown-cotton-fiber varieties with 3 color gradations: Bronesia 1 (light brown), Bronesia 2 (light brown), and Bronesia 3 (dark brown). The potential production of the three varieties are 909 kg, 859 kg, 1,010 kg fiber/ha, respectively. The quality of Bronesian cotton fiber is generally lower compared to the new superior varieties of white fiber cotton (Kanesia 16-Kanesia 23) for fiber length and strength. Cotton fibers of Bronesian varieties are classified as short fibers (23.2-25.7 mm), so that manual and conventional spinning techniques will produce yarn suitable for traditional weaving, especially for woven fabrics with brown based colors.

Influence of the myotome zone and sex on the muscle cellularity and fillet texture of diploid and triploid turbots Scophthalmus maximus L.

The muscle and textural parameters were analyzed in four myotome zones (epaxial upper, hipoaxial upper, epaxial bottom, and hipoaxial bottom) in seven diploids (D) and seven triploids (T) turbot specimens. Diploid specimens showed the highest values of the size and number of white fibers in the epaxial zones, being such values higher in female than male specimens. In triploid specimens, the highest fibers sizes were found in the upper zones (epaxial and hipoaxial), whereas the lowest number and density of fibers were found in the epaxial upper zone. In this latter group (T), the lowest fibers sizes were found in female specimens, whereas the rest of the parameters were usually higher in female than male specimens. When comparing both groups, the hypertrophy was higher in T than D in all zones. In both ploidy groups, the highest textural values were usually observed in the upper epaxial fillet, being slightly higher in female than male specimens. The values of standard length, total weight, gonad weight, gonadosomatic index and gutted weight were higher in female than male specimens in both groups (D and T).

Dietary DHA affects muscle fiber development by activating AMPK/Sirt1 pathway in blunt snout bream (Megalobrama amblycephala)

This study was conducted to evaluate the effect of DHA on muscle development mechanism of blunt snout bream by a feeding trial and a primary hepatocyte culture. Fish (average weight 26.40 ± 0.11 g) were randomly fed three diets: a deficient DHA diet (0% DHA, DD), a control diet (0.1% DHA, DC) and a high level of DHA diet (0.8% DHA, DH) for 8 weeks. Results were indicated that the weight gain rate (WGR) of fish feed with DC diets was significantly higher than the DD and DH diets (P < .05). The texture (hardness, gumminess and chewiness), fibers density, the proportion of small-sized fiber (< 20 μm) and sarcomere lengths in the white muscle were significantly increased in the DH group than DD and DC groups (P < .05). The gene expression of Myog and Mrf4 were significantly increased (P < .05), and the gene expression of MSTNa and MSTNb were significantly decreased in the fish fed DH diets than DD and DC diets (P < .05). These results suggested that DHA increased the flesh quality and promoted the white muscle hyperplasia and muscle fiber development. In addition, the gene expression of AMPKα2 and Sirt1 in the DC and DH group was significantly higher than the DD group (P < .05). The protein level of AMPK, phosphor-AMPK and AMPK/phosphor-AMPK were significantly increased in DC and DH group (P < .05). In vitro, the isolated primary hepatocytes from blunt snout bream were incubated with 0, 100 and 400 μM DHA for 24 h. The results showed that 400 μM DHA was significantly up-regulate the gene expression of AMPKα2 and Sirt1, and increased the protein level of AMPK, phosphor-AMPK, AMPK/phosphor-AMPK and Sirt1 (P < .05). In summary, DHA promoted the white muscle hyperplasia and muscle fiber development, which may be in association with the activation of AMPK/Sirt1 pathway.