Classification Of White Blood Cells Research Articles

Using deep DenseNet with cyclical learning rate to classify leukocytes for leukemia identification.

The examination, counting, and classification of white blood cells (WBCs), also known as leukocytes, are essential processes in the diagnosis of many disorders, including leukemia, a kind of blood cancer characterized by the uncontrolled proliferation of carcinogenic leukocytes in the marrow of the bone. Blood smears can be chemically or microscopically studied to better understand hematological diseases and blood disorders. Detecting, identifying, and categorizing the many blood cell types are essential for disease diagnosis and therapy planning. A theoretical and practical issue. However, methods based on deep learning (DL) have greatly helped blood cell classification. Images of blood cells in a microscopic smear were collected from GitHub, a public source that uses the MIT license. An end-to-end computer-aided diagnosis (CAD) system for leukocytes has been created and implemented as part of this study. The introduced system comprises image preprocessing and enhancement, image segmentation, feature extraction and selection, and WBC classification. By combining the DenseNet-161 and the cyclical learning rate (CLR), we contribute an approach that speeds up hyperparameter optimization. We also offer the one-cycle technique to rapidly optimize all hyperparameters of DL models to boost training performance. The dataset has been split into two sets: approximately 80% of the data (9,966 images) for the training set and 20% (2,487 images) for the validation set. The validation set has 623, 620, 620, and 624 eosinophil, lymphocyte, monocyte, and neutrophil images, whereas the training set has 2,497, 2,483, 2,487, and 2,499, respectively. The suggested method has 100% accuracy on the training set of images and 99.8% accuracy on the testing set. Using a combination of the recently developed pretrained convolutional neural network (CNN), DenseNet, and the one fit cycle policy, this study describes a technique of training for the classification of WBCs for leukemia detection. The proposed method is more accurate compared to the state of the art.

UR-Net: An Integrated ResUNet and Attention Based Image Enhancement and Classification Network for Stain-Free White Blood Cells.

The differential count of white blood cells (WBCs) can effectively provide disease information for patients. Existing stained microscopic WBC classification usually requires complex sample-preparation steps, and is easily affected by external conditions such as illumination. In contrast, the inconspicuous nuclei of stain-free WBCs also bring great challenges to WBC classification. As such, image enhancement, as one of the preprocessing methods of image classification, is essential in improving the image qualities of stain-free WBCs. However, traditional or existing convolutional neural network (CNN)-based image enhancement techniques are typically designed as standalone modules aimed at improving the perceptual quality of humans, without considering their impact on advanced computer vision tasks of classification. Therefore, this work proposes a novel model, UR-Net, which consists of an image enhancement network framed by ResUNet with an attention mechanism and a ResNet classification network. The enhancement model is integrated into the classification model for joint training to improve the classification performance for stain-free WBCs. The experimental results demonstrate that compared to the models without image enhancement and previous enhancement and classification models, our proposed model achieved a best classification performance of 83.34% on our stain-free WBC dataset.

Integrating explainability into deep learning-based models for white blood cells classification

White blood cells (WBCs) are crucial constituents of the blood that protect the human body against infections and viruses. The classification of WBCs in a blood smear image is used to diagnose a range of haematological disorders. The manual identification of WBCs can result in potential errors, necessitating the need for automated systems that can assist in classification. Recently, various deep learning models, such as DenseNet121, Xception, MobileNetV2, ResNet50, and VGG16, have been used to classify WBCs. However, the available classification models are black boxes because their decisions are difficult for humans to understand without further exploration. The interpretability and explainability of these models are essential, as their decisions can have severe consequences for patients. In this paper, we integrate an explainable AI (XAI) technique called local interpretable model-agnostic explanations (LIME) with the DenseNet121 classification model for WBC classification. Interpretable results would allow the users to understand and verify the model’s predictions, enhancing their confidence in the automated diagnosis.

An Explainable Vision Transformer Model Based White Blood Cells Classification and Localization.

White blood cells (WBCs) are crucial components of the immune system that play a vital role in defending the body against infections and diseases. The identification of WBCs subtypes is useful in the detection of various diseases, such as infections, leukemia, and other hematological malignancies. The manual screening of blood films is time-consuming and subjective, leading to inconsistencies and errors. Convolutional neural networks (CNN)-based models can automate such classification processes, but are incapable of capturing long-range dependencies and global context. This paper proposes an explainable Vision Transformer (ViT) model for automatic WBCs detection from blood films. The proposed model uses a self-attention mechanism to extract features from input images. Our proposed model was trained and validated on a public dataset of 16,633 samples containing five different types of WBCs. As a result of experiments on the classification of five different types of WBCs, our model achieved an accuracy of 99.40%. Moreover, the model's examination of misclassified test samples revealed a correlation between incorrect predictions and the presence or absence of granules in the cell samples. To validate this observation, we divided the dataset into two classes, Granulocytes and Agranulocytes, and conducted a secondary training process. The resulting ViT model, trained for binary classification, achieved impressive performance metrics during the test phase, including an accuracy of 99.70%, recall of 99.54%, precision of 99.32%, and F-1 score of 99.43%. To ensure the reliability of the ViT model's, we employed the Score-CAM algorithm to visualize the pixel areas on which the model focuses during its predictions. Our proposed method is suitable for clinical use due to its explainable structure as well as its superior performance compared to similar studies in the literature. The classification and localization of WBCs with this model can facilitate the detection and reporting process for the pathologist.

Classification of white blood cells based on modified U‐Net and SVM

SummaryManual investigation of blood cell count is sometimes erroneous due to interoperability error, fatigue error, requiring expert skill and time consuming too. In particular, investigation of white blood cell (WBC) gains importance in identifying diseases like leukemia, leukopenia, etc. WBC does not possess regular structure because they move throughout the blood stream and hence analyzing WBC and its types for structure and shape is quite challenging. To aid in hematology, this work provides classification of WBC classification based on modified U‐Net and support vector machines (SVM). A modified U‐Net architecture is developed to segment WBC followed by feature extraction and classification by radial basis function‐support vector machine (RBF‐SVM). Experiments indicated that the modified U Net segmentation can detect the WBC nucleus with a dice similarity coefficient of 0.972. The proposed U‐Net‐SVM can recognize WBCs in Raabin‐WBC, LISC, and BCCD datasets with an accuracy of 99.45%, 98.62%, and 98.81%, respectively. Further investigation on leukemia dataset, ALL‐IDB2, revealed an accuracy of 99.42% with 100% sensitivity and specificity. The proposed model can be used to investigate WBCs and hence provide a great support to the hematologists in analyzing the blood smear for various disease identifications.

Automatic base-model selection for white blood cell image classification using meta-learning

Healthcare has benefited from the implementation of deep-learning models to solve medical image classification tasks. For example, White Blood Cell (WBC) image analysis is used to diagnose different pathologies like leukemia. However, medical datasets are mostly imbalanced, inconsistent, and costly to collect. Hence, it is difficult to select an adequate model to overcome the mentioned drawbacks. Therefore, we propose a novel methodology to automatically select models to solve WBC classification tasks. These tasks contain images collected using different staining methods, microscopes, and cameras. The proposed methodology includes meta- and base-level learnings. At the meta-level, we implemented meta-models based on prior-models to acquire meta-knowledge by solving meta-tasks using the shades of gray color constancy method. To determine the best models to solve new WBC tasks we developed an algorithm that uses the meta-knowledge and the Centered Kernel Alignment metric. Next, a learning rate finder method is employed to adapt the selected models. The adapted models (base-models) are used in an ensemble learning approach achieving accuracy and balanced accuracy scores of 98.29 and 97.69 in the Raabin dataset; 100 in the BCCD dataset; 99.57 and 99.51 in the UACH dataset, respectively. The results in all datasets outperform most of the state-of-the-art models, which demonstrates our methodology’s advantage of automatically selecting the best model to solve WBC tasks. The findings also indicate that our methodology can be extended to other medical image classification tasks where is difficult to select an adequate deep-learning model to solve new tasks with imbalanced, limited, and out-of-distribution data.

White blood cell classification based on a novel ensemble convolutional neural network framework

White blood cell classification based on a novel ensemble convolutional neural network framework

White blood cell automatic classification using deep learning and optimized quaternion hybrid moments

The structural characteristics of white blood cells (WBCs) can provide important information about human health status. For this reason, over the past four decades, researchers have sought ways to automate the classification and morphological analysis of white blood cells. In the present study, we propose a new method to classify white blood cells into four types: neutrophils, lymphocytes, monocytes, and eosinophils by combining the hybrid discrete moment quaternion approach with deep learning. The proposed classification method is generally divided into two main phases: the first one is called the preprocessing phase, which is devoted to the computation of the image moments to be classified using a new quaternion Meixner-Charlier hybrid moment that is characterized by the following parameters α,β,andφ. In addition, the grey wolf optimization algorithm is used to guarantee high classification accuracy by optimizing the local parameters of the new quaternion Meixner-Charlier hybrid moments. The classification phase, which is the second phase of our proposed convolutional neural network model, is dedicated to introducing image moments. We present various graphical measurements, including receiver operating characteristics, precision-recall curves, and a confusion matrix. To demonstrate the effectiveness of our classification method, we also employ numerical measures such as the F1 score, loss, and accuracy. Our results indicate that the cell types of neutrophils were determined with a 98.53% accuracy rate, lymphocytes with a 98.16% accuracy rate, monocytes with a 97.43% accuracy rate, and eosinophils with a 97.43% accuracy rate.

CUSS-Net: A Cascaded Unsupervised-Based Strategy and Supervised Network for Biomedical Image Diagnosis and Segmentation.

Biomedical image segmentation and classification are critical components in a computer-aided diagnosis system. However, various deep convolutional neural networks are trained by a single task, ignoring the potential contribution of mutually performing multiple tasks. In this paper, we propose a cascaded unsupervised-based strategy to boost the supervised CNN framework for automated white blood cell (WBC) and skin lesion segmentation and classification, called CUSS-Net. Our proposed CUSS-Net consists of an unsupervised-based strategy (US) module, an enhanced segmentation network named E-SegNet, and a mask-guided classification network called MG-ClsNet. On the one hand, the proposed US module produces coarse masks that provide a prior localization map for the proposed E-SegNet to enhance it in locating and segmenting a target object accurately. On the other hand, the enhanced coarse masks predicted by the proposed E-SegNet are then fed into the proposed MG-ClsNet for accurate classification. Moreover, a novel cascaded dense inception module is presented to capture more high-level information. Meanwhile, we adopt a hybrid loss by combining a dice loss and a cross-entropy loss to alleviate the imbalance training problem. We evaluate our proposed CUSS-Net on three public medical image datasets. Experiments show that our proposed CUSS-Net outperforms representative state-of-the-art approaches.

Leukocytes Classification for Leukemia Detection Using Quantum Inspired Deep Feature Selection.

Leukocytes, also referred to as white blood cells (WBCs), are a crucial component of the human immune system. Abnormal proliferation of leukocytes in the bone marrow leads to leukemia, a fatal blood cancer. Classification of various subtypes of WBCs is an important step in the diagnosis of leukemia. The method of automated classification of WBCs using deep convolutional neural networks is promising to achieve a significant level of accuracy, but suffers from high computational costs due to very large feature sets. Dimensionality reduction through intelligent feature selection is essential to improve the model performance with reduced computational complexity. This work proposed an improved pipeline for subtype classification of WBCs that relies on transfer learning for feature extraction using deep neural networks, followed by a wrapper feature selection approach based on a customized quantum-inspired evolutionary algorithm (QIEA). This algorithm, inspired by the principles of quantum physics, outperforms classical evolutionary algorithms in the exploration of search space. The reduced feature vector obtained from QIEA was then classified with multiple baseline classifiers. In order to validate the proposed methodology, a public dataset of 5000 images of five subtypes of WBCs was used. The proposed system achieves a classification accuracy of about 99% with a reduction of 90% in the size of the feature vector. The proposed feature selection method also shows a better convergence performance as compared to the classical genetic algorithm and a comparable performance to several existing works.

Rapid detection of white blood cells using hyperspectral microscopic imaging system combined with Multi-data Faster RCNN

Blood test is a basic medical test item, and the content of white blood cells (WBCs) is directly related to the evaluation of the patient's health and the judgment of serious diseases. However, both manual detection method and traditional image recognition method have the problem of insufficient efficiency and accuracy. The purpose of this study is to propose a fast and accurate WBC detection method, called hyperspectral based Multi-data Faster RCNN. For hyperspectral multi-dimensional sensing information, one-dimensional (1-D) CNN and Faster RCNN were used to model and analyze spectral and image features, respectively. And then, the Multi-data Faster RCNN was built, in which the 1-D CNN network and fusion network layer were added in Faster RCNN to realize the joint detection based on spectral and image fusion features of WBCs. The hyperspectral system coupled with Multi-data Faster RCNN can effectively improve the accuracy of WBCs’ recognition. The average precision (Ap) value and the F1 score of each classification of WBCs were above 0.9, and the mean average precision (mAP) even reached 0.962. The results showed that the Multi-data Faster RCNN integrated with hyperspectral imaging had great potential in the detection of WBCs.

Hyperspectral images classification for white blood cells with attention-aided convolutional neural networks and fusion network

The classification of White blood cells (WBCs) plays an important role. However, the traditional method of blood smear analysis is laborious. This paper presented a classification method of WBCs based on hyperspectral images and Deep learning (DL). The U-net network was proposed to extract spectral features of WBCs region of interest (ROI) under the pseudo-color images with specific hyperspectral images (420.8, 557.2 and 667.4 nm). For spectral features and the pseudo-colour images of hyperspectral data, attention-aided one and two-dimensional convolutional neural networks were applied to establish WBCs classification models, respectively. The overall average accuracy can reach 94.20% and 92.60%, respectively. A fusion network was constructed to make full use of the spectral and image spatial features, and its classification accuracy reached 96.20%. In terms of overall average accuracy, the fusion network model was the optimal, but for individual types of WBCs, each network had its own unique advantages.

Ghost-ResNeXt: An Effective Deep Learning Based on Mature and Immature WBC Classification

White blood cells (WBCs) must be evaluated to determine how well the human immune system performs. Abnormal WBC counts may indicate malignancy, tuberculosis, severe anemia, cancer, and other serious diseases. To get an early diagnosis and to check if WBCs are abnormal or normal, one needs to examine the numbers and determine the shape of the WBCs. To address this problem, computer-aided procedures have been developed because hematologists perform this laborious, expensive, and time-consuming process manually. Resultantly, a powerful deep learning model was developed in the present study to categorize WBCs, including immature WBCs, from the images of peripheral blood smears. A network based on W-Net, a CNN-based method for WBC classification, was developed to execute the segmentation of leukocytes. Thereafter, significant feature maps were retrieved using a deep learning framework built on GhostNet. Then, they were categorized using a ResNeXt with a Wildebeest Herd Optimization (WHO)-based method. In addition, Deep Convolutional Generative Adversarial Network (DCGAN)-based data augmentation was implemented to handle the imbalanced data issue. To validate the model performance, the proposed technique was compared with the existing techniques and achieved 99.16%, 99.24%, and 98.61% accuracy levels for Leukocyte Images for Segmentation and Classification (LISC), Blood Cell Count and Detection (BCCD), and the single-cell morphological dataset, respectively. Thus, we can conclude that the proposed approach is valuable and adaptable for blood cell microscopic analysis in clinical settings.

White Blood Cells Classification Using Entropy-Controlled Deep Features Optimization.

White blood cells (WBCs) constitute an essential part of the human immune system. The correct identification of WBC subtypes is critical in the diagnosis of leukemia, a kind of blood cancer defined by the aberrant proliferation of malignant leukocytes in the bone marrow. The traditional approach of classifying WBCs, which involves the visual analysis of blood smear images, is labor-intensive and error-prone. Modern approaches based on deep convolutional neural networks provide significant results for this type of image categorization, but have high processing and implementation costs owing to very large feature sets. This paper presents an improved hybrid approach for efficient WBC subtype classification. First, optimum deep features are extracted from enhanced and segmented WBC images using transfer learning on pre-trained deep neural networks, i.e., DenseNet201 and Darknet53. The serially fused feature vector is then filtered using an entropy-controlled marine predator algorithm (ECMPA). This nature-inspired meta-heuristic optimization algorithm selects the most dominant features while discarding the weak ones. The reduced feature vector is classified with multiple baseline classifiers with various kernel settings. The proposed methodology is validated on a public dataset of 5000 synthetic images that correspond to five different subtypes of WBCs. The system achieves an overall average accuracy of 99.9% with more than 95% reduction in the size of the feature vector. The feature selection algorithm also demonstrates better convergence performance as compared to classical meta-heuristic algorithms. The proposed method also demonstrates a comparable performance with several existing works on WBC classification.

Classification of Atypical White Blood Cells in Acute Myeloid Leukemia Using a Two-Stage Hybrid Model Based on Deep Convolutional Autoencoder and Deep Convolutional Neural Network.

Recent advancements in artificial intelligence (AI) have led to numerous medical discoveries. The field of computer vision (CV) for medical diagnosis has received particular attention. Using images of peripheral blood (PB) smears, CV has been utilized in hematology to detect acute leukemia (AL). Significant research has been undertaken in the area of AL diagnosis automation in order to deliver an accurate diagnosis. This study addresses the morphological classification of atypical white blood cells (WBCs), including immature WBCs and atypical lymphocytes, in acute myeloid leukemia (AML), as observed in peripheral blood (PB) smear images. The purpose of this work is to build a classification model for atypical AML WBCs based on their distinctive features. Using a hybrid model based on geometric transformation (GT) and a deep convolutional autoencoder (DCAE), this work provides a novel technique in the field of AI for resolving the issue of imbalanced distribution of WBCs in blood samples, nicknamed the "GT-DCAE WBC augmentation model". In addition, to extract context-free atypical WBC features, this study develops a stable learning paradigm by incorporating WBC segmentation into deep learning. In order to classify atypical WBCs into eight distinct subgroups, a hybrid multiclassification model termed the "two-stage DCAE-CNN atypical WBC classification model" (DCAE-CNN) was developed. The model achieved an average accuracy of 97%, a sensitivity of 97%, and a precision of 98%. Overall and by class, the model's discriminating abilities were exceptional, with an AUC of 99.7% and a class-wise range of 80% to 100%.

Retracted: Deep Learning Model for the Automatic Classification of White Blood Cells.

[This retracts the article DOI: 10.1155/2022/7384131.].

Automated Artificial Intelligence Empowered White Blood Cells Classification燤odel

White blood cells (WBC) or leukocytes are a vital component of the blood which forms the immune system, which is accountable to fight foreign elements. The WBC images can be exposed to different data analysis approaches which categorize different kinds of WBC. Conventionally, laboratory tests are carried out to determine the kind of WBC which is erroneous and time consuming. Recently, deep learning (DL) models can be employed for automated investigation of WBC images in short duration. Therefore, this paper introduces an Aquila Optimizer with Transfer Learning based Automated White Blood Cells Classification (AOTL-WBCC) technique. The presented AOTL-WBCC model executes data normalization and data augmentation process (rotation and zooming) at the initial stage. In addition, the residual network (ResNet) approach was used for feature extraction in which the initial hyperparameter values of the ResNet model are tuned by the use of AO algorithm. Finally, Bayesian neural network (BNN) classification technique has been implied for the identification of WBC images into distinct classes. The experimental validation of the AOTL-WBCC methodology is performed with the help of Kaggle dataset. The experimental results found that the AOTL-WBCC model has outperformed other techniques which are based on image processing and manual feature engineering approaches under different dimensions.

White Blood Cell Classification based on Gray Level Co-occurrence Matrix with Zero Phase Component Analysis Approach

This paper presents an approach to WBC classification by employing Gray Level Co-occurrence Matrix (GLCM) along with the Analysis of Variance (ANOVA) test and Zero Phase Component Analysis (ZCA) whitening. Moreover, the performance is evaluated through the K- Nearest Neighbor (K-NN) classifier. The proposed approach has achieved an accuracy of 94.25% on the Blood Cell Count and Detection (BCCD) dataset for the classification of four categories of WBC namely, lymphocytes, monocytes, neutrophils, and eosinophils. The experimental results reveal an improvement in accuracy (11.05, 8.15, and 14.25%) in comparison to the state-of-the-art approaches i.e., Watershed segmentation, Local Binary Pattern (LBP), and Extreme Learning Machines (ELMs) respectively.

Automatic Classification of White Blood Cells Using Pre-Trained Deep Models

White blood cells (WBCs), which are part of the immune system, help our body fight infections and other diseases. Certain diseases can cause our body to produce fewer WBCs than it needs. For this reason, WBCs are of great importance in the field of medical imaging. Artificial intelligence-based computer systems can assist experts in the analysis of WBCs. In this study, an approach is proposed for the automatic classification of WBCs over five different classes using a pre-trained model. ResNet-50, VGG-19, and MobileNet-V3-Small pre-trained models were trained with ImageNet weights. In the training, validation, and testing processes of the models, a public dataset containing 16,633 images and not having an even class distribution was used. While the ResNet-50 model reached 98.79% accuracy, the VGG-19 model reached 98.19% accuracy, the MobileNet-V3-Small model reached the highest accuracy rate with 98.86%. When the predictions of the MobileNet-V3-Small model are examined, it is seen that it is not affected by class dominance and can classify even the least sampled class images in the dataset correctly. WBCs were classified with high accuracy using the proposed pre-trained deep learning models. Experts can effectively use the proposed approach in the process of analyzing WBCs.

CLASSIFICATION OF CYTOLOGICAL IMAGES OF WHITE BLOOD CELLS USING DEEP LEARNING

Leukocytes, also known as white blood cells, are an important part of the immune system and represent a group of cells that protect the body from infections. The classification of leukocytes is widely used to diagnose various diseases, such as AIDS, leukemia, myeloma, anemia and others. However, traditional methods of classification of leukocytes require a lot of time, and their results are prone to errors. The article implements a computer approach to classification and detection of white blood cells on images of blood cells using deep learning methods based on the application of the method of transferring deep learning models and finetuning them. Deep learning has become one of the most popular areas of artificial intelligence. There are many neural network architectures used in deep learning in solving computer vision problems. The purpose of this study is to develop a computer system for efficient automatic detection and classification of leukocytes of four types of blood cells: eosinophils, lymphocytes, monocytes, neutrophils. This article used popular models of accurate neural networks VGG16, ResNet50, DensNet201, MobileNetV3, InceptionResnetV2, pretrained on ImageNet and finetuned on WBC images dataset.The paper also proposes a custom model of a deep neural network using separable convolutional blocks SeparableConv2D in its architecture. The model is optimized using methods of preprocessing normalization and data augmentation. The is model gave classification metrics of accuracy = 98.84 %, precision = 99.56 %, recall = 98.89 % and f1-score = 99.22 %. The developed model allows, in most cases, to determine with high speed whether a leukocyte belongs to one of the four classes in the image, which indicates the possibility of using the system as an auxiliary tool for hematological blood analysis.