Whereas White Blood Cell Research Articles

Longitudinal ventricular cerebrospinal fluid profile in patients with spontaneous subarachnoid hemorrhage.

BackgroundSpontaneous subarachnoid hemorrhage (SAH) is a severe neurological disease that frequently requires placement of external ventricular drainage (EVD). Cerebrospinal fluid (CSF) obtained via the drain is used to detect potential complications of SAH.ObjectiveThis study aimed to describe the longitudinal profile of routine CSF parameters in patients with SAH and to identify associations with neurological complications.MethodsA total of thirty-three patients with spontaneous SAH who required an EVD and had at least three consecutive CSF samples collected over a period of more than 7 days were included in this study.ResultsA median of 6 longitudinally collected CSF samples per patient were available within 1–22 days after SAH onset. Overall, red blood cells (RBC) steadily decreased over time, whereas white blood cells (WBC) and total protein (TP) increased until days 6 and 13, respectively, and decreased thereafter. The estimated decay rates of RBC, WBC, and TP were 28, 22, and 6% per day. Distinct CSF patterns over time were linked to known complications after SAH. Patients with rebleeding showed increased RBC, TP, and phagocytosing cells compared to patients without re-bleeding. For ventriculitis, an elevated cell index with a higher proportion of granulocytes was characteristic. CSF of patients with delayed cerebral ischemia showed increased RBC and WBC compared to patients without DCI. Early CSF WBC and cell index were predictive for the occurrence of DCI and ventriculitis later during the disease course. The amount of daily CSF drainage via EVD had no impact on routine CSF parameters.ConclusionLongitudinal CSF characteristics are associated with SAH-related complications.

The Use of Predictive Laboratory Tests in Rapid Triage of COVID-19 Pandemic Outpatient Clinic Patients.

Patients who come to the emergency pandemic outpatient clinic with a pre-diagnosis of COVID-19 are still a burden on the health system. Rapid triage of patients is important to reduce transmission. The aim of this study is to evaluate the biochemistry and hemogram results of real-time reverse transcription-polymerase chain reaction (RT-PCR) positive and negative patients in the emergency pandemic outpatient clinic and to investigate predictive values of the initial tests that will help to make rapid diagnosis. Patients who applied to the emergency pandemic outpatient clinic with the suspicion of COVID-19 between November 01, 2020 and January 01, 2021 were evaluated with RT-PCR and laboratory examinations. A total of 551 patients were included in the study. The mean age of the patients was 50.31 ± 18.47 (min. 18 - max. 94), and 47.2% (n = 260) of the patients included in the study were male and 52.8% (n = 291) were female. In the comparison of hemogram parameters, we found that mean platelet volume (MPV) was significantly higher (p = 0.023), whereas white blood cell (WBC), platelet counts (PLT), lymphocyte and neutrophil values were significantly lower in RT-PCR positive patients (p < 0.001). There were no significant differences between the PCR positive and negative patients in terms of other parameters. In the comparison of biochemical parameters, we found that lactate dehydrogenase LDH (p = 0.001), creatinine (p = 0.002), and AST (p < 0.001) values were significantly higher in PCR positive patients, while there were no significant differences in terms of other biochemical parameters (p > 0.05). Our study results show that the practical quick-look hemogram and MPV can be used as a specially evaluated parameter in the rapid management of the first application COVID-19 patients. In addition, biochemically high levels of LDH and creatinine can be used to guide the clinician in terms of early hydration of the patient with a pre-diagnosis of COVID-19 to alleviate acute kidney damage.

Hematological Parameters and Blood Smear Effects of Tramadol on Male Rabbits

Abstract: Analgesics are a bunch of drugs that act totally different ways on the central apprehensive framework to absense of pain and alleviation from torment. It is in this manner imperative to ponder the impacts of organization these analgesics to decide the side impacts for them utilize. Hence we decided to study the hematological effects of tramadol on male rabbits. Tenth rabbits were arbitrarily partitioned into two break even with bunches (each bunch five rabbits). The primary gather was utilized as a control. The moment gather was utilized to consider the impact of tramadol (50 mg/kg body weight) for six weeks. Hemoglobin (Hb) substance, ruddy blood cells (RBCs) number and hematocrit (Hct) esteem diminished, whereas white blood cells (WBCs) and platelets tallies expanded after tramadol organization. Verbal organization of tramadol induced significant changes within the morphology of erythrocytes. In rabbit treated with tramadol the cells were appeared change in typical shape from star molded to sickle cells. The ponder appeared the destructive impacts of tramadol on the hematological parameters and blood spread on mail rabbits.

Effect of Allium sativum methanol extract in amelioration of arsenic-induced toxicity in Swiss albino mice

Background: Arsenic (As) toxicity is a major public health concern in many countries worldwide. Chronic arsenic toxicity results in multiple organ failure, cancer, neurological disorder, behavioral disorder, reproductive complications, etc. Allium sativum (garlic) has been reported to ameliorate mercury, lead, and cadmium-induced toxicity previously. Purpose: This research was aimed to evaluate the effect of methanol garlic extract in the amelioration of arsenic toxicity in Swiss albino mice. Methods: Initially 36 Swiss albino mice were divided into 6 different groups such as control group, arsenic-treated group where dose of arsenic was 0.0075 mg/mL, and arsenic plus methanolic extract of garlic treated group, and mice were treated at three different doses of extract; 150, 300, and 500 mg/kg for 8 weeks. Finally, the change in body weight, hematological, and biochemical parameters were measured. Results: Progressive body weight increase was observed in the control and only extracts treated groups whereas retardation of the body weight gain was found in the arsenic treated group. A decrease in the body weight gain was retarded in the groups that received arsenic plus garlic extract (GE). Compared to control, neutrophil (NEU), and eosinophil (EOS) counts as well as alanine aminotransferase (ALT), alkaline phosphatase (ALP), creatinine (CRE), and serum glutamic-pyruvic transaminase (SGPT) levels were increased whereas white blood cell (WBC), red blood cell (RBC), lymphocyte (LYM), and monocyte (MON) count were decreased in the arsenic-treated group. All these parameters were returned to normal levels in the garlic methanol extract-treated group. Conclusion: This study suggests that garlic has the potential for depleting arsenic-induced toxicity and thus, dietary intake of garlic might have therapeutic benefits in the prevention or amelioration of arsenic-exposed toxicity.

Sumithion induced structural erythrocyte alteration and damage to the liver and kidney of Nile tilapia.

Indiscriminate use of pesticides in agricultural land poses a potential threat to many non-target organisms, including fish. In the present study, we explored the toxicological effects of sumithion on Nile tilapia (Oreochromis niloticus) after exposure at different concentrations (0.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0 mg/L) in triplicate for 96 h. The 96-h LC50 value of the pesticide for the test fish was calculated by probit analysis, which was 2.579 mg/L. We also investigated the haematological parameters, erythrocytic alteration and histopathological responses of Nile tilapia. Red blood cell (RBC) and haemoglobin (Hb) level were significantly declined, whereas white blood cell (WBC) and blood glucose level were increased dramatically in the sumithion treated fish. The frequencies of erythrocytic cellular and nuclear alterations were significantly elevated in the fish after exposure to sumithion compared with the control group. The substantial histopathological alterations were observed in sumithion-exposed fish, including pyknotic nucleus, melano-macrophage centers and severe congestion of blood vessels in the liver and patch degeneration, vacuolation and intense form of pyknotic nuclei in the kidney. Observed alterations in this study exhibit that sumithion negatively impacts on Nile tilapia. It is recommended that the use of this pesticide in the agriculture field be monitored and controlled.

Toxicological evaluations of betulinic acid and ursolic acid; common constituents of Houttuynia cordata used as an anthelmintic by the Naga tribes in North-east India

BackgroundBetulinic acid (BA) and ursolic acid (UA) are two major phytoconstituents of Houttuynia cordata Thunb., (Saururaceae) which is used as an anthelmintic in the traditional medicine system of the Nagas in Nagaland, India. This study evaluates their toxic potentials using rodent models (Swiss albino mice and Wistar rats) according to the OECD (Organization for Economic Cooperation and Development) guidelines. Acute and 28-day sub-acute oral toxicity studies were conducted, and evaluations were made based on biochemical, hematological, and histopathological observations.ResultsAcute oral toxicity study revealed the oral LD50 of both the test compounds to be > 2000 mg/kg in mice. Sub-acute administration of BA at 10 mg/kg body weight (b.w.) revealed a significant increase in serum glutamic oxaloacetic transaminase (SGOT), alkaline phosphatase (ALP), urea concentrations and eosinophil and lymphocyte counts in rats. Animals administered with 10 mg/kg b.w. UA revealed elevated neutrophil count, SGOT, ALP, and urea concentrations, whereas white blood cells (WBC), lymphocyte, and platelet counts were found to be low. Histopathological examinations of body organs revealed alterations in the architecture of the liver, kidney, and spleen tissues. Notably, all these alterations were recoverable as evident in the satellite group, indicating a recovering pattern from the toxic effects caused by the oral administration of these phytocompounds.ConclusionAlthough UA and BA possess several therapeutic properties, their long-term usage can cause mild toxicity in their users. This study also paves way for evaluating the optimum effective and safe dose of these phytocompounds.

Chromium Exposure Causes Structural Aberrations of Erythrocytes, Gills, Liver, Kidney, and Genetic Damage in Striped Catfish Pangasianodon hypophthalmus.

Heavy metal pollution due to anthropogenic activities poses a great threat to aquatic organisms. The present study was conducted to evaluate the cytotoxic and genotoxic effects of hexavalent chromium (potassium dichromate) on hemato-biochemical, histo-pathological, and genetical changes in striped catfish Pangasianodon hypophthalmus. Three sub-lethal doses (0.8, 1.6, and 3.2 mg/L) of chromium (Cr) were selected and fish were exposed in vivo contrasting with a control (0 mg/L) for 30 days. The study revealed that various hemato-biochemical parameters showed a significant decrease in hemoglobin (Hb), red blood cells (RBCs), and blood glucose content, whereas white blood cells (WBCs) significantly increased in Cr exposed fish. Frequencies of all forms of structural abnormalities of erythrocytes (erythrocytic cellular abnormalities; ECA, erythrocytic nuclear abnormalities; ENA and erythroblasts; Ebs) were significantly increased in higher two test concentrations (1.6 and 3.2 mg/L) when compared to control. Differential leucocyte count exhibited significant increase in neutrophil and decrease in lymphocytes in the highest Cr treated group. The severity of various histo-pathological changes in the gills, liver, and kidney were increased considerably with the increase of Cr concentrations. Similarly, the amount of DNA (ng/μl) decreased significantly in blood and tissues of different vital organs where the liver showed the highest decline compared to control in a concentration-dependent manner. Taken altogether, P. hypophthalmus is susceptible to Cr and can be used as a bio-indicator to assess aquatic metal pollution.

Abstract A25: In vivo evidence validating the palmitoylation/depalmitoylation cycle as a therapeutic target in NRAS mutant hematologic cancers

Abstract A dynamic cycle of palmitoylation and depalmitoylation stably localizes N-Ras, but not K-Ras-4b, at the plasma membrane. This selective dependency suggests that targeting palmitoylation could inhibit the growth of NRAS-mutant cancers while leaving normal cells unaffected, due to their intact K-Ras-4b function. We sought to clarify the dependency of oncogenic N-Ras signaling on palmitoylation in a model relevant to human cancer. We used the lox-STOP-lox (LSL) knock-in system (Haigis et al., Nat Genet 2008) to express an NrasG12D oncogene at physiologic levels, and generated a second site mutation abrogating the palmitoylation site (C181S). We then compared Mx1-Cre; NrasLSL-G12D,C181S/LSL-G12D,C181S to Mx1-Cre; NrasWT/WT and Mx1-Cre; NrasLSL-G12D/LSL-G12D (hereafter G12D,C181S, WT, and G12D, respectively). We injected mice with poly(I:C) at weaning to excise the LSL cassette in hematopoietic cells. As reported (Wang et al., Blood 2011; Xu et al., Cancer Disc 2013), G12D mice died early (119-267 days, n=14) from an aggressive myeloproliferative disorder (MPD). In contrast, no mortality was observed in the G12D,C181S and WT cohorts until 18-19 months of age (p=3x10-6 by Kaplan-Meier analysis). Pathologic analysis of 6-month-old mice showed that key features of MPD were fully rescued in the G12D,C181S cohort. Specifically, whereas white blood cell (WBC) counts and spleen sizes were in the normal range in G12D,C181S and WT mice, G12D mice had profound leukocytosis (median WBC count: 52.19 k/μL), splenomegaly (median spleen weight: 0.884 g), and myeloid expansion (n=6). Biochemical analysis revealed that N-RasG12D,C181S accumulated in the GTP-bound conformation, but mostly localized in the cytosol, and failed to hyperactivate the MAPK pathway. We next generated NrasG12D,C181S/G12D compound heterozygous mice to investigate potential interactions between oncogenic NrasG12D alleles with and without the C181S mutation in vivo. Over half of these mice developed hematologic disease and died prematurely, although later than homozygous G12D mutant mice (median survivals: 429 and 225 days; n = 22 and 14, respectively). Common findings in moribund mice were splenomegaly, expansion of myeloid cells, and different degrees of leukocytosis and anemia. Importantly, genetic analysis from diseased mice revealed reduced frequency or absence of the NrasG12D,C181S allele (allelic burden 0-30%) due to secondary somatic genetic events. This observation supports the existence of high selective pressure to overcome growth-suppressive properties of unpalmitoylated N-Ras during leukemogenesis. In conclusion, these studies provide strong genetic evidence validating palmitoylation as a therapeutic target in NRAS mutant hematologic malignancies and establish a novel model for addressing this question in other cancers. Citation Format: Noemi A. Zambetti, Ari J. Firestone, Jasmine C. Wong, Amanda M. Long, Anagha Inguva, Jarrett R. Remsberg, Radu M. Suciu, Benjamin F. Cravatt, Kevin M. Haigis, Kevin Shannon. In vivo evidence validating the palmitoylation/depalmitoylation cycle as a therapeutic target in NRAS mutant hematologic cancers [abstract]. In: Proceedings of the AACR Special Conference on Targeting RAS-Driven Cancers; 2018 Dec 9-12; San Diego, CA. Philadelphia (PA): AACR; Mol Cancer Res 2020;18(5_Suppl):Abstract nr A25.

Home enteral nutrition may prevent myelosuppression of patients with nasopharyngeal carcinoma treated by concurrent chemoradiotherapy.

The aim of this study is to assess the effect of home enteral nutrition (HEN) on the myelosuppression of patients with nasopharyngeal cancer (NPC) during the course of concurrent chemoradiotherapy (CCRT). A total of 18 outpatients with NPC administered oral nutritional supplementation intervention at home during the course of CCRT were designated as the HEN group, whereas 36 patients with NPC who had previously completed CCRT were retrospectively included as the control group. Patient Generated Subjective Global Assessment, body mass index (BMI), and blood test were evaluated prior to CCRT. During the course of CCRT, blood test was assessed every 2 weeks. In male patients, hemoglobin (HB) and red blood cell were decreased (P < .05) in both HEN and control group after CCRT, whereas white blood cell (WBC) started to decrease since week 2 of CCRT in the control group but maintained in the HEN group which was significantly higher than the control (5.05 ± 1.29 vs 3.77 ± 1.5, P < .05). In female patients, HB and WBC were reduced in control group during CCRT, whereas these indicators also maintained in the HEN group. Surprisingly, all patients with lower BMI (<24 kg/m2 ) had a significant increase in platelet (PLT) after CCRT (200.78 ± 58.03 vs 253.00 ± 69.82, P < .05), while had steady HB and WBC values in the HEN group. At the end of CCRT, WBC and PLT of the HEN group were both higher than those in the control group (5.21 ± 1.07 vs 3.37 ± 1.52), (253.00 ± 69.82 vs 165.57 ± 59.56) (P < .05 for both). Our findings suggest that HEN is effective in preventing myelosuppression during CCRT for patients with NPC. Our findings suggest that HEN is effective in preventing myelosuppression during CCRT for patients with NPC.

Toxicity bioassay and sub-lethal effects of diazinon on blood profile and histology of liver, gills and kidney of catfish, Clarias gariepinus.

The catfish, Clarias gariepinus, was exposed to different acute concentrations (5-10 mg/l) of diazinon and the Lc50 value was recorded as high as 7.3 mg/l for 96 hours. The fishes exposed to three sub-lethal levels of diazinon (0.73, 1.095 and 1.46 mg/l) for 30 days showed that the pesticide induces changes in different blood parameters. Number of red blood cells (RBC), haemoglobin level and haematocrit values were elevated whereas white blood cells (WBC) count was reduced. Various blood indices like mean corpuscular volume (MCV), mean corpuscular haemoglobine (MCH) and mean corpuscular haemoglobine concentration (MCHC) vary insignificantly in the fish treated with diazinon. Glucose level and activity level of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) was increased in Clarias gariepinus exposed to diazinon. Protein level in plasma of fish treated with pesticide was changed but the change was insignificant. Histological changes in the liver, gills and kidney of fishes exposed to diazinon were apparent when compared to control. Hepatocytes necrosis and bleeding were more distinct in the fishes exposed to pesticide. Glomerulus hypertrophy and bleeding in kidneys; and fusion and degeneration of secondary lamellae and epithelial hyperplasia in gills were also observed in the exposed fish.

Hematological, hepatic enzymes' activity and oxidative stress responses of gray mullet (Mugil cephalus) after sub-acute exposure to copper oxide.

Given the importance of assessing potential toxicity of heavy metals in valuable species of aquatic animals, the goal of the present research was to assess the sub-acute effects of copper oxide on hematological, enzymological, and oxidative stress responses of gray mullet to measure toxicity of copper oxide pollution in this significant fish. The median lethal concentration (LC50) value of copper oxide to gray mullet was detected at 3.15±0.039mg/L for 96h, and 25 and 50% of the 96-h LC50 values were selected as sub-acute concentrations. The fish were exposed to (0.79 and 1.57mg/L) copper oxide for 21days. At the end of 21days, the results showed that hemoglobin (Hb), hematocrit (Hct), red blood cells (RBC) count, MCV, MCH, and MCHC levels were found to be decreased in copper oxide treated fish, whereas white blood cells (WBC) count increased in copper-treated fish. Plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) activity increased in treated groups; however, copper oxide in both groups of sub-acute exposure significantly decreased plasma alkaline phosphatase (ALP) activity compared to the control group. Superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) levels significantly declined in copper oxide-treated fish. These findings indicated the deleterious effects of copper oxide on gray mullet, even at low concentrations, and offered that hematological and hepatic enzyme activity and antioxidants are suitable tools for evaluating heavy metals toxicity.

Mean platelet volume in children with hepatitis A.

BackgroundMean platelet volume (MPV), which is commonly used as a measure of platelet size, indicates the rate of platelet production and platelet activation. We aimed to evaluate the mean platelet volume in children with hepatitis A.MethodsIn this retrospective case-controlled study, the study population consisted of 62 children with hepatitis A and 62 healthy control subjects.ResultsMPV values, aspartate transaminase (AST), and alanine transaminase (ALT) levels on admission were significantly increased in patients with hepatitis A when compared to controls whereas white blood cell (WBC) counts were significantly lower. Two weeks after admission, the MPV values showed a significant decrease from 9.47 ± 1.62 to 8.84 ± 1.48 fL in patients with hepatitis A, but these values were still significantly higher than the controls. There was a significant difference in terms of MPV, WBC, AST, and ALT values between the controls and the patient group 2 weeks after admission.ConclusionsThis study is the first to evaluate the MPV levels in children with hepatitis A. MPV values were found to be increased in children hospitalized with hepatitis A.

Effect of photografting 2-hydroxyethyl acrylate on the hemocompatibility of electrospun poly(ethylene-co-vinyl alcohol) fibroporous mats

Poly(ethylene-co-vinyl alcohol) (EVAL) has been recommended as a material suitable for blood contacting applications. Effect of ethylene content and associated hydrophobicity of EVAL on the blood–material interactions have been documented in the literature. In this work, surface chemistry of EVAL substrate was altered by photografting a hydrophilic monomer, 2-hydroxyethyl acrylate (HEA) with the aid of a photoinitiator, benzophenone (BP), and the effect of surface modification on the blood–material interactions was investigated. Since the modified material was intended to be used as leukodepletion filters, a solution containing EVAL, HEA and BP was electrospun into fibroporous mats and UV treated to induce grafting. Degree of grafting, bonding between fibers and fiber diameter increased with increase in UV exposure time whereas mechanical properties showed a decreasing trend. Decreased water contact angle indicated improved wetting characteristics. In vitro hemocompatibility tests revealed that the modified EVAL surface exhibited significantly lower hemolytic activity, protein adsorption and platelet adhesion than neat EVAL. The modification did not have any substantial effect on the activation of the complement system and coagulation parameters. Photografting led to significant reduction in the adhesion of red blood cells (RBC) whereas white blood cell (WBC) consumption remained above 90%. The results implied that photografting HEA on EVAL substantially improves hemocompatibility of EVAL and when it is used as a filter, it selectively removes leukocytes and allows easy passage of other blood components.

English

The common carp (Cyprinus carpio) was exposed to diazinon to determine the lethal concentration (LC50) values at different times and effects of sub-lethal concentrations on haematological and biochemical parameters. The LC50&nbsp;values at varying times were recorded as high as 18.57 mg/l (24 h), 14.87 mg/l (48 h), 12.29 mg/l (72 h) and 9.76 mg/l (96 h). Fishes exposed to sub-lethal concentrations (0.5, 1.0 and 2.0 mg/l) for four weeks revealed that the pesticide causes alterations in various blood parameters. Red blood cell (RBC) counts, haemoglobin concentration and haematocrit values increased whereas white blood cell (WBC) count decreased. Plasma glucose level, glutamate-oxaloacetate transminase (PGOT) and plasma glutamate pyruvate transminase (PGPT) activities increased in the fish exposed to diazinon. Protein level in plasma and glycogen in liver and muscles were found to decrease in treated fish with diazinon.&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; &nbsp; keyword:&nbsp;Bioassay, Biochemical and Haematological Changes, Diazinon,Cyprinus carpio

Effect of atrazine (Herbicide) on blood parameters of common carp Cyprinus carpio (Actinopterygii: Cypriniformes)

In the present study an attempt was made to investigate the acute toxicity of atrazine (ATR) a herbicide on an economically important fish, Cyprinus carpio. In which, 24 h median lethal concentration (24 h LC50) of herbicide, atrazine to C. carpio was 18.5 ppm. Due to fish exposure to 24 h LC50 of atrazine for 24 h, red blood cells (RBCs) count (-63.17%), hemoglobin (-27.35%), plasma glucose (-6.78%) and plasma protein (-18.73%) levels were decreased; whereas white blood cells (WBCs) count (+3.73%) was enhanced. The differences in haematological and biochemical values were statistically significant (p < 0.05). However, WBC count was not significantly changed. The alterations of the above parameters could be used as an important tool for the assessment of pathological conditions of fish. Key words: Atrazine, acute toxicity, hematology, biochemical, Cyprinus carpio.

Hematological and biochemical changes in organically farmed sheep naturally infected with Fasciola hepatica

A naturally occurring outbreak of fasciolosis in a group of 20 Merinolandschaf (German Merino) sheep was studied. Hematological and blood biochemical values in sheep spontaneously infected with liver fluke Fasciola hepatica were compared with equivalent values in 20 parasite-free sheep from organically farmed flock. Investigated animals were kept in outdoor system, on pastures covered with swamps, which remain flooded after rainy season. Significantly lower red blood cell (RBC) count, lymphocytes, hemoglobin (Hb), packed cell volume (PCV), mean corpuscular hemoglobin concentration (MCHC), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine, and albumin were recorded in sheep from the infected herd, whereas white blood cell (WBC) count, eosinophil, segmented and band neutrophil count, mean corpuscular volume (MCV), gamma-glutamyl transferase (GGT), concentrations of glucose, and globulins were significantly higher than in the parasite free herd. No significant correlation between the investigated blood parameters and the number of F. hepatica eggs in the feces was detected. This study shows that hematological and biochemical values can be useful in early diagnosis and prognosis of sheep fasciolosis.

Observations on Rinderpest

Poly(ethylene-co-vinyl alcohol) (EVAL) has been recommended as a material suitable for blood contacting applications. Effect of ethylene content and associated hydrophobicity of EVAL on the blood–material interactions have been documented in the literature. In this work, surface chemistry of EVAL substrate was altered by photografting a hydrophilic monomer, 2-hydroxyethyl acrylate (HEA) with the aid of a photoinitiator, benzophenone (BP), and the effect of surface modification on the blood–material interactions was investigated. Since the modified material was intended to be used as leukodepletion filters, a solution containing EVAL, HEA and BP was electrospun into fibroporous mats and UV treated to induce grafting. Degree of grafting, bonding between fibers and fiber diameter increased with increase in UV exposure time whereas mechanical properties showed a decreasing trend. Decreased water contact angle indicated improved wetting characteristics. In vitro hemocompatibility tests revealed that the modified EVAL surface exhibited significantly lower hemolytic activity, protein adsorption and platelet adhesion than neat EVAL. The modification did not have any substantial effect on the activation of the complement system and coagulation parameters. Photografting led to significant reduction in the adhesion of red blood cells (RBC) whereas white blood cell (WBC) consumption remained above 90%. The results implied that photografting HEA on EVAL substantially improves hemocompatibility of EVAL and when it is used as a filter, it selectively removes leukocytes and allows easy passage of other blood components.