Wheat Triticum Aestivum Research Articles

Effect of Different Thermal Regimes and Moisture Levels on the Growth Parameters of Wheat (Triticum aestivum L.) Crop Grown under Agro-climatic Conditions of Eastern Uttar Pradesh, India

Effect of Different Thermal Regimes and Moisture Levels on the Growth Parameters of Wheat (Triticum aestivum L.) Crop Grown under Agro-climatic Conditions of Eastern Uttar Pradesh, India

Performance of low-input agriculture with ZBNF-A case study on groundnut (Arachis hypogaea L.)–wheat (Triticum aestivum L.) cropping system in Saurashtra region of Gujarat

Sustainable low-cost farming in (groundnut based) cropping system offers a much-needed alternative to conventional input-intensive agriculture. Keeping this in view a field experiment was conducted during rainy (kharif) season of 2022–23 at ICAR-Directorate of Groundnut Research, Junagadh, Gujarat on groundnut (Arachis hypogaea L.) to evaluate the performance of low-input agriculture with ZBNF (Zero-budget natural farming) vis-avis others. Experiment was conducted in a split plot design having 3 different farming practices, viz. Natural farming (NF); Integrated crop management (ICM or conventional farming); and Organic farming (OF) practices in main plot using 2 cultivars ‘GJG-22’ (Virginia Bunch) and ‘TG 37A’ (Spanish Bunch) in large sub plot (~1/100th of a hectare), replicated 8-times. Further, these permanent plots of 3 farming practices were cultivated with wheat (Triticum aestivum L.) (two cultivars, viz. ‘GW 451’ and ‘Lok 1’) in rotation during winter (rabi) season of 2022–23 following a residual exhaustive fodder crop of pearl millet [Pennisetum glaucum (L.) R.Br.] grown during spring/summer 2021– 22 prior to the experimentation. The study revealed that pod yield to the tune of 1,316–1,630 kg/ha was realized with low cost NF (or ZBNF) practice (higher yields with Spanish Bunch) vis-a-vis 1,448–1,779 kg/ha in OF practice. On the contrary, higher pod yield of 1,679–1,965 kg/ha was realized with the ICM practice employing improved production technologies. The growth/developmental parameters/values including economics and total system productivity were also higher under ICM practice. Furthermore, higher values in respect of all the groundnut plant attributes including economics and nutrient (NPK) uptake, and its efficiency (NPK uptake/100-kg pod) were evident under ICM practice. Similar crop responses were conspicuous in case of the succeeding wheat crop. Thus, overall crop response to farming practices were in the order: ICM>OF>NF; and the ratio of N, P, and K uptake for realization of higher per unit productivity (across farming practices and varieties) was observed to be 6.8:1.0:2.2 for groundnut and 3.9:1.0:3.3 for wheat. Thus, ICM practice comprising of judicious use of chemical fertilizers, manure and pesticides use was advantageous for realizing higher yield and return in groundnut-wheat. Further efforts are made to realize higher productivity of ecofriendly farming practices (ZBNF and OF).

Effect of zinc and boron on grain quality of wheat (Triticum aestivum) in north-western plain zone of India

An experiment was conducted to study the “Effect of zinc and boron on grain quality of wheat (Triticum aestivum L.) during the winter (rabi) season of 2018–2019 and 2019–20 at the agronomy research farm of IFTM University, Lodhipur Rajput, Moradabad, Uttar Pradesh. Ten treatments, viz. T1 , Control (no fertilizer); T2 , zinc @ 5 kg/ha (SA); T3 , zinc @ 1 kg/ha (FA); T4 - zinc @ 1 + 1 kg/ha (2 FA); T5 , zinc @ 5 kg/ha (SA) + zinc @ 1 kg/ha (FA); T6 , boron @ 0.5 kg/ha (SA); T7 , boron @ 0.1 kg/ha (FA); T8 , boron @ 0.2 kg/ha (2 FA); T9 , boron @ 0.5 kg/ha (SA) and boron @ 0.1 kg/ha (FA) and T10-, zinc @ 5 kg/ha + boron @ 0.5 kg/ha (SA) were comprised in randomized block design (RBD) with the three replications. The results revealed that different levels and application methods of zinc and boron significantly improved the grain quality of wheat. Maximum Zn content (35.4 and 35.9 mg/ha) and Zn uptake (185.1 and 197.1 g/ha) in 2018–19 and 2019–20, respectively were recorded with Zn @ 5 kg/ha (SA) + zinc @ 1 kg/ha (FA). Moreover, maximum B content (58.5 and 59.5 mg/ha) and B uptake (361.9 and 376.4 g/ha) in 2018–19 2019–20, respectively were observed with B @ 0.5 kg/ha (SA) and B @ 0.1 kg/ha (FA). Maximum protein content in grain (12.6 and 12.7%) and protein yield (712.8 and 744.9 kg/ha) was recorded under Zn - 5 kg/ha (SA) + B 0.5 kg/ha (SA) in 2018–19 and 2019–20, respectively

Halophilic microbial formulations for improving crop productivity and soil health under saline Vertisols of Gujarat

A study was carried out during winter (rabi) seasons of 2018 and 2019 at research farm of ICAR-Central Soil Salinity Research Institute, Regional Research Station, Bharuch, Gujarat to find out the effect of various halophilic microbial formulations in combination of organic manure and chemical fertilizers on winter (rabi) wheat (Triticum aestivum L.) productivity under saline Vertisols. Experiment was conducted in a randomized block design (RBD) comprised of 5 treatments, viz. T1 , Un-inoculated + Vermicompost @2.5 t/ha + 100% RDF (recommended dose of fertilizers); T2 , Un-inoculated + Vermicompost @2.5 t/ha + 75% RDF; T3 , Vermicompost @2.5 t/ha + 75% RDF + Halo-Azo inoculation; T4 , Vermicompost @2.5 t/ha + 75% RDF + Halo-PSB inoculation; and T5 , Vermicompost @2.5 t/ha + 75% RDF + Mix of Halo-Azo and Halo-PSB inoculation, replicated thrice. Results showed that application of different halophilic microbial formulations like Halo-Azo (Azotobacter sp. and Bacillus sp.) and Halo-PSB (consortia of Eneterobacter and Brevibacterium sp.) with vermicompost @2.5 t/ha + 75% RDF (recommended dose of fertilizers), provided at par yield with full dose (120 : 60 : 00; N : P : K kg/ha) of fertilizers, thus able to reduce 25% chemical fertilizer dose. Halo-Azo formulation performed better for improving crop yield as compared to Halo-PSB formulation. Combined application of Halo-Azo and Halo-PSB with vermicompost @2.5 t/ha and 75% RDF performed the best and provided significantly higher grain yield (2.75 t/ha) as compared to other treatments. Single and combined application of Halo-Azo and Halo-PSB with vermicompost @2.5 t/ha and 75% RDF also improved soil health parameters and significant results, i.e. declined EC (2.46 dS/m) and pH (7.33), improved organic carbon (0.69%) and nutrient availability (available N: 299.8 kg/ha and P: 28.7 kg/ha) were obtained. Results showed that integration of halophilic microbial formulations with organics and chemical fertilizers was able to improve wheat yield up to 15% than conventional farming under saline Vertisols of Gujarat.

Climate change shocks and crop production: The foodgrain bowl of India as an example

Global warming is causing climate change (CC) characterized by increased frequency of heatwaves, droughts, erratic rains, hailstorms, cloudbursts, floods, landslides etc. The CC has already adversely affected ecosystems. In spite of efforts to mitigate greenhouse gas (GHG) emissions, which lead to warming, the global temperature during 2011–20 was 1.1°C above that during pre-industrial era. The projections are that warming will continue to increase and adverse effects will intensify particularly in developing countries like India. In India a number of studies have recorded wide spatial variability in rainfall, though, many reported a general overall negative trend since mid-20th century. Further, varying pattern of rainfall has been recorded in three agroclimatic regions of Punjab state, the granary of India. Unseasonal rains followed by spiked temperature during winter (rabi) season of 2021–22 reduced wheat (Triticum aestivum L.) yield in Punjab by 651 kg/ha and by 301 kg/ha in Haryana compared to 2020–21. Further, the grain was of lower quality. During rainy (kharif) season of 2022, Southern Rice Black-streaked Dwarf Virus (SRBSDV), appeared for the first time in Punjab and Haryana. Some farmers ploughed the affected fields. Adverse weather during rabi 2022–23 also, reduced the wheat yield (143–150 kg/ha) in these states. At the national level, erratic weather during rabi 2021–22 and kharif 2022 caused losses of about 3 mt of grain of each of wheat and rice (Oryza sativa L.). The projected increased adverse effects due to intensified CC include food insecurity. Thus, there is an emergent need to accelerate implementation of adaptation and mitigation strategies in agriculture. The adaptation options include cultivar improvement, conservation agriculture altering growing seasons, crop diversification and sustainable soil, and water resource management. In the process of adaptive management of crop production, adjusting sowing dates and breeding cultivars having varying duration in consonance with CC has been one of the central aspects. Shifting sowing dates to find appropriate crop cultivation season is a low-cost measure. However, cultivar development is time and resource consuming. Novel biotechnological tools enable fast cultivar development with precision, and facilitate mobilization of genes from wild-weedy relatives, which are rich in genes conferring resistance/tolerance to biotic and biotic stresses, required to combat CC challenge. In view of CC stress on water resources, improving water-use efficiency (WUE) has gained importance. Sensor-based micro-irrigation/fertigation has great potential to enhance water and fertilizer-use efficiency. Similarly, the application of other smart technologies like nanotechnology, sensor-based pesticide application, bio-fertilizers and bio-pesticides, need to be mobilised. In view of agro-ecological diversity in India, right-sized regionspecific technology packages have to be developed implying that crop research will expand exponentially. This needs strengthening of human resources and institutional infrastructure, expanding and linking basic and applied researches, and fortifying inter-disciplinary/inter-institutional collaborations to develop and diffuse technology innovations. Enabling factors include enhanced funding and international cooperation. All-out efforts are needed to have more climate-resilient agriculture.

Chickpea (Cicer arietinum)-based intercropping systems in Rajasthan’s Hadoti region: Productivity and economic viability

A study was carried out during three successive winter (rabi) seasons (2018–19 to 2020–21) at the Agricultural Research Station in Ummedganj, Kota, Rajasthan to determine the productivity and economic viability of intercropping systems based on chickpea (Cicer arietinum L.) in the Hadoti region of Rajasthan. Nine various chickpeabased intercropping systems, viz. Sole chickpea; Sole linseed (Linum usitatissimum L.); Sole coriander (Coriandrum sativum L.); Sole wheat (Triticum aestivum L.); Sole mustard [Brassica juncea (L.) Czern.]; Chickpea + linseed (6:2); Chickpea + coriander (6:2); Chickpea + wheat (6:2); and Chickpea + mustard (6:2) were investigated in a randomized block design (RBD) with three replications. According to combined statistics, the chickpea + mustard intercropping system had the highest chickpea grain yield and equivalent yield (1,865 kg/ha and 2,574 kg/ ha, respectively) when compared to all other intercropping systems. Results of competitive indices revealed that compared to rest of the intercropping system, chickpea + mustard (6:2) recorded significantly higher LER (1.25), MAI (18,462) and SPI (2912 kg/ha), while the lowest land equivalent ratio (LER), monetary advantage index (MAI), system productivity index (SPI) and production efficiency (PE) were recorded in the chickpea + wheat intercropping system. The chickpea + mustard intercropping system yielded values of competition ratio (3.56), aggressivity (-0.05), system profitability index (2912), relative crowding coefficient (13.2), and area time equivalent ratio (1.24), that were sustainable and producible in comparison to other treatments. In terms of economics, compared to all other intercropping and solo crops, the chickpea + mustard intercropping system yielded the chickpea’s most significant net returns (`93,681/ha) and benefit cost (B:C) ratio (3.11). So, for south-eastern Rajasthan, the mustard and chickpea intercropping system is a productive and sustainable system.

Preceding kharif crops and fertilizer influence on growth, yield and nutrient requirement of succeeding wheat (Triticum aestivum)

A field experiment was conducted during 2 consecutive rainy (kharif) and winter (rabi) season each of 2020–21 and 2021–22 at C.P. College of Agriculture, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat to study the effect of preceding kharif crops and fertilizer on growth, yield, economics and nutrient requirement of succeeding wheat (Triticum aestivum L.) crop. The experiment was laid out in split plot design comprising of 4 kharif crops, viz. greengram [Vigna radiata (L.) R. Wilczek], sesame (Sesamum indicum L.), pearl millet [Pennisetum glaucum (L.) R.Br.] and fodder sorghum [Sorghum bicolor (L.) Moench] as main plots and 5 fertilizer treatments, viz. 100% RDF (120 kg N + 60 kg P2 O5 + 40 kg K2 O/ha); 75% RDF; 50% RDF; 75% RDF + Azotobacter + PSB (phosphate solubilizing bacteria); and 50% RDF + Azotobacter + PSB as sub plots with four replications. Wheat grown after kharif greengram had better growth parameters and yield attributes which was reflected in an increase in grain (4.78 t/ha) and straw (6.41 t/ha) yield of wheat. Among different fertilizer treatments, wheat fertilized with 100% RDF recorded significantly higher grain (4.60 t/ha) and straw yield (6.20 t/ha). Nutrient uptakes by grain and straw increased under wheat grown after greengram and fertilized with 100% RDF. Greengramwheat crop sequence and wheat fertilized with either 100% RDF or 75% RDF along with Azotobacter + PSB seed inoculation earned higher net realization and B:C ratio.

Microbial population and yield of rice–wheat system under variable irrigation and nutrient management

A field experiment was conducted during rainy (kharif) season of 2018–19 and winter (rabi) season of 2019–20 at the Water Management Research Farm of CSK Himachal Pradesh Agricultural University, Palampur, Kangra, Himachal Pradesh to study the effect of moisture and nutrient management practices on different soil microbial entities and crop yields in rice (Oryza sativa L.)–wheat (Triticum aestivum L.) system. Experiment was laid out in a split-plot design with 3 replications. Treatments comprised of 3 irrigation levels, viz. Recommended critical stages regimes; Irrigation at 0.8 CPE (cumulative pan evaporation) (rice) and 0.6 CPE (wheat); Irrigation at 1.0 CPE (rice) and 1.0 CPE (wheat) assigned to main-plots and 4 nutrient management practices, viz. Inorganic; Organic; Natural farming; and Integrated management allotted to sub-plots. Irrigation at critical stages resulted in significantly higher rice yield, bacterial, azotobacter, phosphate solubilizing bacteria (PSB), actinomycetes population. However, fungi population increased under higher moisture regimes. In case of wheat crop, irrigation at 1.0 CPE resulted in a significantly higher yield (7.5% higher over irrigation at critical stages) in both the years of study. Organic and integrated nutrient management practices being statistically at par with each other recorded significantly higher microbial population. A significantly higher fungi population was, however, recorded with natural farming. The highest crop yields were obtained with integrated nutrient management (3.4 t/ha rice and 3.6 t/ha wheat in second year). The best combination for rice was irrigation at critical stages along with integrated nutrient management. Whereas, for wheat irrigation at 1.0 CPE with same nutrient management proved to be the best. Hence it is recommended to follow integrated nutrient management and irrigation at critical stages in rice and at 1.0 CPE in wheat for higher productivity as well as for improved biological properties of soil.

Multi-species weed detection and variable spraying system for farmland based on W-YOLOv5

Weed infestations have the potential to cause significant economic losses for farmers as a result of diminished crop yields and escalated labor and input costs linked with weed management. Traditional weed control methods often entail indiscriminate application of herbicides across entire fields, irrespective of weed density or species composition. In this investigation, we propose a hierarchical detection algorithm for identifying multi-species weeds and have devised a variable spraying system in agricultural settings. The weed classification detection algorithm comprises two components: crop seedling target detection and weed detection. The enhanced YOLOv5 algorithm was initially merged with Vision Transformer (ViT) to introduce the W-YOLOv5 crop seedling target detection algorithm. Experimental validation results indicate that the mean average precision (mAP) of the proposed W-YOLOv5 stands at 87.6%, representing a 3.2% increase over the original YOLOv5. Compared with YOLOv7, this method demonstrates a 4.4% improvement in mAP, accompanied by an 80.14% reduction in floating point operations (FLOPs). These findings underscore the effectiveness of the proposed crop seedling target detection algorithm in ensuring detection accuracy while minimizing model FLOPs. Subsequently, following the detection of crop seedlings, the Hue, Saturation, and Value (HSV) color space filtering algorithm was employed to identify the locations of all weeds in the image, thereby facilitating the detection of weeds among wheat (Triticum aestivum L.), radish (Raphanus sativus L.), cucumber (Cucumis sativus L.), soybean (Glycine max (L.) Merr.), and corn (Zea mays L.) seedlings. Finally, the severity of weed infestation in farmland was classified into five levels. Leveraging these severity levels, we developed a variable spraying system and integrated the developed algorithm into the system. Field validation experiments demonstrate that at a speed of 4 km/h, the spraying accuracy of the system can reach 90.32%, effectively enabling precise variable spraying.

Responses of soil aggregation and aggregate‐associated nitrogen to straw return in China: Evidence from a meta‐analysis and a pot experiment

AbstractEnhancing soil structure and soil nitrogen availability is the key to sustainable agricultural development in global cropping systems. Straw return can affect soil aggregation, but the results of recent studies on the influence of straw return on soil aggregation are not consistent, and the distribution and availability of straw‐derived nitrogen in soils are still unclear. Here, a meta‐analysis of the effects of straw return on soil aggregation was conducted based on 57 studies published in China over the past 10 years. Moreover, combined with a pot experiment, the distribution and transfer characteristics of the two main wheat (Triticum aestivum L.)‐preceding crops, corn (Zea mays L.) and soybean (Glycine max L.) straw‐derived nitrogen in the soils were studied by using the 15N‐tracer method in the main wheat‐producing areas of China. 15N‐labeled straw was introduced into soils in pots at rates of 0.3% and 0.5% of the soil dry mass and incubated 60%–70% of the field water capacity throughout the entire growth period of wheat (220 days). A meta‐analysis revealed that straw return significantly improved the mass ratio of large macro‐aggregates (LM%) and small macro‐aggregates (SM%) but significantly decreased the mass ratio of micro‐aggregates (MI%) and silt plus clay‐size particles (CS%), with mean effect sizes of 36.27%, 9.06%, −8.26%, and −21.32%, respectively. The mean weight diameter (MWD) and geometric mean diameter (GMD) also increased by 21.48% and 15.64%, respectively. Compared with SM%, LM% made a greater contribution to the stability of soil aggregates. Moreover, only LM% showed a significant positive correlation with the corresponding aggregate‐associated nitrogen content, which was an important reason for the improvement in soil nitrogen availability after straw return. The positive effect of straw return on soil aggregation was greatest when the soil pH was close to 6.5–7.5, the average annual temperature was greater than 15°C, and the average annual rainfall was close to 800–1000 mm. The results of the pot experiment revealed that the proportions of straw‐derived nitrogen in >2 mm aggregates were 1.21–1.28, 1.50–2.23, and 1.34–1.74 times greater than those in 0.25–2, 0.053–0.25, and <0.053 mm aggregates, respectively. Moreover, the proportion of straw‐derived nitrogen content of aggregates of different particle sizes showed that the proportion of straw used to reach 0.5% of the soil dry weight was greater than 0.3%, which was 1.09–1.66 times greater, and that of soybean straw was significantly greater than that of corn straw, which was 1.50–2.28 times greater. At application rates of 60 g pot−1 and 100 g pot−1 straw, the absorption of soybean straw‐derived nitrogen by wheat was 1.83 and 2.15 times greater than that of corn straw‐derived nitrogen, respectively. In conclusion, straw return effectively improved soil aggregate stability and nitrogen availability, which was closely related to the increase in soil LM%. Soybean straw can stabilize soil aggregates better than corn straw, and its straw‐derived nitrogen is an important nitrogen source for subsequent wheat crops.

Composition of high-molecular-weight glutenin subunits and gluten quality in wheat lines with alien genetic material

Background. High-molecular-weight glutenin subunits (HMW-GSs) make the greatest contribution to the formation of baking properties in bread wheat (Triticum aestivum L.). Glutenin polymorphism of bread wheat relatives is significantly richer than in cultivated varieties. The objective of this work was to identify the HMW-GS composition and assess the gluten quality of bread wheat lines with introgressions of alien genetic material.Materials and methods. We studied the parental varieties of spring bread wheat, accessions of the tetraploid and hexaploid Triticum L. species, and 19 introgressive lines produced with their participation. Glutenins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis and HMW-GSs were identified using the Payne nomenclature system. Gluten quality was determined in accordance with GOST 13586.1-68 (the 2017–2019 and 2021–2022 growing seasons). Data processing was performed using the Statistica 10.0 and MS Excel software packages.Results and conclusion. HMW-GSs uncharacteristic of T. aestivum cultivars were identified in the studied accessions of T. dicoccoides (Körn. ex Aschers. et Graebn.) Schweinf., T. spelta L., and T. kiharae Dorof. et Migusch. These HMW-GSs are of interest for enriching the wheat gene pool. HMW-GSs of related species were found in 10 out of 19 introgressive lines. A five-year observation period revealed that wheat relatives on average significantly exceeded common wheat cultivars in gluten quality, and introgressive lines did not differ significantly from the parental genotypes. Lines with high rheological properties of gluten had, as a rule, HMW-GSs of related species in their composition.

Early changes in carbon uptake and partitioning moderate belowground carbon storage in a perennial grain

There is increasing interest in perennial crops to build soil carbon (C), but the mechanisms underlying soil C accrual in perennial croplands remain unclear, especially over time in the first years of perennial crop growth. To address this gap, research is needed that directly tracks intra-annual C fluxes through crop-microbial-soil pools, evaluating the capacity of perennial crops to build soil C over intra-decadal time periods. We conducted a 13C isotope-tracer study to compare within-season C uptake and crop-microbial-soil C partitioning patterns between 1-year-old (IWG-1) and 2-year-old (IWG-2) stands of a novel perennial grain crop, intermediate wheatgrass (IWG; Thinopyrum intermedium (Host) Barkworth and Dewey). We compared these to a common annual grain crop, spring wheat (Triticum aestivum L.). Crop shoots, roots, soil, and soil respired-C were sampled ten times over a 90-day chase period. We also measured the incorporation of recently assimilated 13C into soil microbial biomass (13C PLFA) and functional groups over the first 7 days post-label application. Overall, IWG-1 assimilated almost 1670 mg 13C m−2 during the study period, nearly twice that of IWG-2 or wheat, but neither IWG system retained significant amounts of new C in soil. Rather, a higher proportion of assimilated new C was retained in IWG-1 in root tissues (14%) and arbuscular mycorrhizal fungi when compared to other cropping systems, while IWG-2 retained almost 50% of total assimilated C in aboveground crop tissues. We expect the shift from new C retention in belowground root-mycorrhizal networks to aboveground tissues is associated with a shift from an acquisitive to conservative growth strategy that occurs between the first and second IWG production years. The observed shift in C partitioning patterns and potential change in growth strategy limited the allocation and retention of new C in soil as IWG aged, adding valuable context to our understanding of why perennial grain crop establishment seldom leads to significant carbon gains in the first several years following establishment.

Discovery of Fusarium pseudograminearum Causing Crown Rot of Winter Wheat in Xinjiang Uygur Autonomous Region, China.

Fusarium pseudograminearum is an important plant pathogen that invades many crops (Zhang et al. 2018). Since it was first discovered in Australia in 1951, F. pseudograminearum has been reported in many countries and regions and caused huge economic losses (Burgess et al. 2001). In 2012, crown rot of wheat caused by F. pseudograminearum was discovered for the first time in Henan Province, China (Li et al. 2012). Wheat (Triticum aestivum L.) is one of the most important food crops in Xinjiang Uygur Autonomous Region (XUAR), with 1.07 million hectares cultivated in 2020. In June 2023, a survey of crown rot disease was carried out in winter wheat cv. Xindong 20 in Hotan area, XUAR, China (80.148907°E, 37.051474°N). About 5% of wheat plants showed symptoms of crown rot such as browning of the stem base and white head. The disease was observed in 85% of wheat fields. In order to identify the pathogens, 36 pieces of diseased stem basal tissue, 0.5 cm in length, were collected and sterilized with 75% alcohol for 30s and 5% NaOCl solution for 2 min, then rinsed three times with sterile water and placed on potato dextrose agar (PDA) medium at 25°C. A total of 27 isolates with consistent morphological characteristics were obtained using single-spore technique (Leslie and Summerell. 2006), and the isolation rate was 75%. The isolates grew rapidly on PDA, produced large numbers of fluffy white hyphae, and pink pigment accumulated in the medium. The isolates were grown on 2% mung bean flour medium and identified by morphological and molecular methods. Macroconidia were abundant, relatively slender, curved to almost straight, commonly two to seven septate, and averaged 22 to 72 × 1.8 to 4.9 μm. Microconidia were not observed. The morphological characters are consistent with Fusarium (Aoki and O'Donnell. 1999). Two isolates (LP-1 and LP-3) were selected for molecular identification. Primers EF1/EF2 (5'-ATGGGTAAGGARGACAAGAC-3'/5'-GGARGTACCAGTSATCATG-3') were used to amplify a portion of the EF-1α gene (O'Donnell et al. 1998). The two 696 bp PCR products were sequenced and submitted to GenBank. The EF-1α gene sequences (GenBank Accession No: PP062794 and PP062795) shared 99.9% identity (695/696) with published F.pseudograminearum sequences (e.g., OP105187, OP105184, OP105179, OP105173). The identification was further confirmed by F. pseudograminearum species-specific PCR primers Fp1-1/Fp1-2 (Aoki and O'Donnell. 1999). The expected PCR products of 518 bp were produced only in F. pseudograminearum. Pathogenicity tests of LP-1 and LP-3 isolates were performed on 7-day-old seedlings of winter wheat cv. Xindong 20 using the drip inoculation method with a 10-μl of a 106 macroconidia ml-1 suspension near the stem base (Xu et al. 2017). The experiment was repeated five times in a 20 to 25°C greenhouse. Control seedlings were treated with sterile water. After 4 weeks, wheat seedling death and crown browning occurred in the inoculated plants with over 90% incidence. No symptoms were observed in the control plants. The pathogen was reisolated from the inoculated plants by the method described above and identified by morphological and PCR amplification using F. pseudograminearum species-specific primers Fp1-1/Fp1-2. No F. pseudograminearum was isolated from the control plants, fulfilling Koch's postulates. To our knowledge, this is the first report of F. pseudograminearum causing crown rot of winter wheat in XUAR of China. Since F. pseudograminearum can cause great damage to wheat, one of the most important food crops in China, necessary measures should be taken to prevent the spread of F. pseudograminearum to other regions.

Effect of herbicides on mixed weed flora in wheat (Triticum aestivum L.) and weed control efficiency

Effect of herbicides on mixed weed flora in wheat (Triticum aestivum L.) and weed control efficiency

Proteomics profiling reveals the detoxification and tolerance behavior of two bread wheat (Triticum aestivum L.) varieties under arsenate stress

Arsenic (As) contaminated irrigation water caused a significant buildup of its level in the agricultural soil and edible tissues of wheat. High levels of As negatively impact grain quality, wheat yield, and human health consequently. It is still unknown how wheat varieties respond to As stress at the proteomic level. Therefore, the present study aimed to deliver mechanistic details regarding the role of proteins in tolerance mechanism against As stress. Two wheat varieties (BARANI-70 and NARC-2009) were exposed to arsenate treatment (200µM) in a hydroponic experiment to evaluate the impacts of arsenic stress using proteomics. Roots of both varieties were used for protein extraction, digestion, and LC-MS/MS analysis to identify differentially abundant proteins (DAPs). Furthermore, various bioinformatic tools were used to study subcellular localization, functional categories, and pathway analysis. The findings identified 67 DAPs in BARANI-70 (up-regulated=56; down-regulated=11) and 89 DAPs in NARC-2009 (up-regulated=34; down-regulated=55). Subcellular localization revealed significant up-regulation of DAPs in mitochondria and chloroplast for BARANI-70 but down-regulation in NARC-2009 under As stress. Functional analysis revealed significant upregulation of DAPs for glutathione, peroxidase, glutathione peroxidase, lactoylglutathione lyase, and glutathione-s-transferase in BARANI-70. Pathway analysis showed regulation of DAPs involved in tetrapyrrole biosynthesis and glycolysis-TCA particularly in BARANI-70 compared to NARC-2009. Furthermore, BARANI-70 showed better growth response under As stress compared to NARC-2009. Thus, better synthesis of stress-responsive DAPs and important pathways in BARANI-70 contributed towards its tolerance against As stress. The present research supports the use of tolerant varieties and identified protein biomarkers to enhance crop resilience against As stress.

Co-expression network analysis and identification of core genes in the interaction between wheat and Puccinia striiformis f. sp. tritici.

The epidemic of stripe rust, caused by the pathogenPuccinia striiformisf. sp.tritici(Pst), would reduce wheat (Triticum aestivum) yields seriously. Traditional experimental methods are difficult to discover the interaction between wheat and Pst. Multi-omics data analysis provides a new idea for efficiently mining the interactions between host and pathogen. We used 140 wheat-Pst RNA-Seq data to screen for differentially expressed genes (DEGs) between low susceptibility and high susceptibility samples, and carried out Gene Ontology (GO) enrichment analysis. Based on this, we constructed a gene co-expression network, identified the core genes and interacted gene pairs from the conservative modules. Finally, we checked the distribution of Nucleotide-binding and leucine-rich repeat (NLR) genes in the co-expression network and drew the wheat NLR gene co-expression network. In order to provide accessible information for related researchers, we built a web-based visualization platform to display the data. Based on the analysis, we found that resistance-related genes such as TaPR1, TaWRKY18 and HSP70 were highly expressed in the network. They were likely to be involved in the biological processes of Pst infecting wheat. This study can assist scholars in conducting studies on the pathogenesis and help to advance the investigation of wheat-Pst interaction patterns.

TESTING OF WHEAT GENOTYPES FOR SALT TOLERANCE AND LEAF RUST DISEASE CAUSED BY PUCCINIA

Parameters that show a significant genotypic variation and are associated with salt tolerance may be used as rapid and economic screening criteria in breeding programs. The objective of this study was to test growth and yield components for evaluating the salt tolerance of wheat genotypes. Five genotypes of winter wheat (Triticum aestivum L.) were used in this study, that differ from their salt tolerance, which were grown in 28 dS/m saline soil, and irrigated by well water with a salinity 7.5 dS/m. The results showed that salt concentration in the soil was reduced with plant growth stages from 28 dS/m before sowing to 8, 7.5, and 7.6 dS/m for N1, N2, and N3 genotypes, respectively. Whereas approached 16 and 17 dS/m for Tumos2 and Mexipak, cultivars, respectively, at the maturity stage. Concerning germination percentage under saline conditions, wheat genotypes N1, N2, and N3 showed the highest percentages of 89, 90, and 90%, respectively, which was significantly different than wheat cultivars Tumos2 and Mexipak 79 and 83%, respectively. Statistical analysis of the data revealed that genotype N2 required a maximum days for germination 14 days, whereas cultivarTumos2 required less days for germination 12 days. For spikes formation duration growth the genotype N3 was the late 119 days, whereas for physiological maturity N1 genotype was the latest 153 days. The number of spikes per 6 m2, grains spike-1, and grain weight were reduced significantly in sensitive cultivars Tumos2 and Mexipak. Higher grain yield with N2 genotype 2739.43 g with no significant differences with the genotypes N1 and N2, and with significant differences with the rest sensitive cultivars Tumos2 and Mexipak 346.61 and 242.98 g, respectively. Therefore, we conclude that the measurements of growth and yield components may be effective criteria for screening wheat genotypes for salt tolerance. Moreover, N1, N2, and N3 genotypes were identified as the most salt-tolerant genotypes in this study, they can be utilized through appropriate selection and breeding programs for further improvement in salt tolerance of Iraqi wheat genotypes.

Selection indices and character association studies in wheat (Triticum aestivum L.) under late sown condition

Selection indices and character association studies in wheat (Triticum aestivum L.) under late sown condition

Selectivity of the Premixtures Flufenacet, Diflufenican and Flufenacet, Diflufenican, Metribuzin on Bread Wheat (Triticum aestivum L.) and Barley (Hordeum vulgare L.) and Efficacy on ALS/ACCase-Resistant Populations of Lolium rigidum Gaudin

The premixtures flufenacet plus diflufenican and flufenacet plus diflufenican plus metribuzin are two herbicides recently registered in Greece for weed control in bread wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) with application early post-emergence to the crop (1st–3rd leaf growth stage). To evaluate the selectivity of these new herbicides, pot experiments were conducted by applying flufenacet plus diflufenican at 240 + 120 g ai ha−1 and flufenacet plus diflufenican plus metribuzin at 119.7 + 119.7 + 44.8 g ai ha−1 to bread wheat and barley, at 1st (BBCH-11), 2nd (BBCH-12) and 3rd (BBCH-13) leaf growth stage. The efficacy of the herbicides at the above-mentioned rates in pre-emergence application was also tested on three ALS/ACCase herbicide-resistant populations of Lolium rigidum Gaudin in comparison with the pre-emergence herbicides prosulfocarb and chlorotoluron plus diflufenican at 3200 g ai ha−1 and 1380 + 92 g ai ha−1, respectively. The results revealed decreased selectivity of both premixtures when applied at BBCH-11 for both winter cereals, with flufenacet plus diflufenican being less selective compared to flufenacet plus diflufenican plus metribuzin. Both herbicides highly controlled the three herbicide-resistant L. rigidum populations. The results indicated that both premixtures are effective chemical options for the management of herbicide resistant L. rigidum. To ensure crop safety and optimize efficacy, application at BBCH-12 is recommended.

Genome-wide identification and examination of the wheat glycosyltransferase family 43 regulation during Fusarium graminearum infection

In Arabidopsis and rice, the glycosyltransferase (GT) 43 family is involved in xylan synthesis. However, there have been limited reports on the study of the TaGT43 family in wheat. In this study, 28 TaGT43 family members were identified in wheat (Triticum aestivum L.) and clustered into three major groups based on the similarity of amino acid sequences. The results of the TaGT43 family's conserved motif and gene structure analyses agree with this result. Collinearity analysis revealed segmental duplications mainly promoted TaGT43 family expansion. cis-Acting element analysis revealed that the TaGT43 genes were involved in the light response, phytohormone response, abiotic/biotic stress response, and growth and development. The TaGT43 family showed a tissue-specific expression pattern, primarily expressed in roots and stems. Besides, the transcriptional and expression levels of multiple TaGT43 genes were upregulated during the infection of F. graminearum. According to metabolomics studies, F. graminearum infection affected the phenylpropanoid biosynthesis pathway in wheat, a critical factor in cell wall construction. Furthermore, GO enrichment analysis indicated that the TaGT43 genes play a significant role in cell wall organization. Based on these results, it may be concluded that the TaGT43 family mediates cell wall organization in response to F. graminearum infection.