Wheat Rye Research Articles

Development of a new resource-saving technology of rye-wheat bread on liquid rye sourdoughs using recycled bread

Recycling of substandard bread, technological waste and bread from the distribution network is a promising way of waste management and raw materials saving. In order to optimize the old bread recycling process and to improve the quality of finished products, a new resource-saving technology for rye–wheat bread was developed. It was found that use under 25% of bread crumbs instead of flour was possible in a liquid sourdough with a moisture content of 70%. When old bread dosage increased from 50 to 100%, acid accumulation slowed down, the lifting force deteriorated, the viscosity increased, which may makes it difficult or impossible to pump the sourdough in industrial technological process. The liquid sourdough made with 25% of old bread had a better lifting capacity and contained more alcohol and volatile acids than the control. The quality of bread made using sourdough with 25% of old bread was comparable in quality to control bread made using traditional sourdough. Liquid rye sourdough with recycled old bread allowed the bread shelf life extension by slowing down the bread staling. No effect on mold rate was found when using recycled old bread in sourdough.

Feral rye control in quizalofop‐resistant wheat in central Great Plains

AbstractFeral rye (Secale cereale L.) is one of the most troublesome winter annual grass weed species in semi‐arid winter wheat (Triticum aestivum L.) production systems across the central Great Plains (CGP). Lack of effective herbicide options further complicates the selective control of feral rye in winter wheat. The main objectives of this research were: (a) determine the sensitivity response of 10 feral rye populations collected from Kansas wheat fields to quizalofop‐p‐ethyl in a dose‐response assay, and (b) to determine the effectiveness of various rates and application timings (fall or spring) of quizalofop‐p‐ethyl for feral rye control in quizalofop‐resistant winter wheat in the CGP. Dose‐response assays indicated that all tested feral rye populations were highly sensitive to quizalofop‐p‐ethyl with GR90 values (doses of quizalofop‐p‐ethyl needed for 90% reductions in shoot biomass at 3 wk after treatment) ranging from 33 to 72 g a.i. ha−1. A field study conducted in Colorado, Kansas, and Oklahoma for a total of 5 site‐years indicated that quizalofop‐p‐ethyl applied at ≥77 g a.i. ha−1 in the fall or spring provided season‐long feral rye control at 92–100% in quizalofop‐resistant winter wheat. Irrespective of tested rates and application timings, a quizalofop‐p‐ethyl treatment protected winter wheat yield loss of 17–27% due to reduced feral rye competition compared to the nontreated weedy check in Colorado and Oklahoma. Results suggested that quizalofop‐p‐ethyl at labelled use rates (77–92 g a.i. ha−1) applied in fall or spring can provide effective feral rye control in quizalofop‐resistant winter wheat.

The Effect of Dietary Rye Inclusion and Xylanase Supplementation on Structural Organization of Bone Constitutive Phases in Laying Hens Fed a Wheat-Corn Diet.

Simple SummaryConsumption of diets with a high-level incorporation of rye leads to an increase in the concentration of nonstarch polysaccharides, which cannot be utilized by poultry. The enzyme xylanase degrades arabinoxylans, the most common nonstarch polysaccharides present in both wheat and rye. In this study, the effect of dietary hybrid rye inclusion and enzyme xylanase supplementation to a diet of laying hens on bone quality was evaluated. Bone quality in laying hens is especially important as one of the bone functions is to store calcium and other minerals for eggshell production. The results of our study showed that modern hybrid rye varieties, when supplemented with exogenous xylanase, can be introduced to the diet of laying hens without any adverse effects on bone structure.This study was conducted to examine the effect of dietary rye inclusion and xylanase supplementation on the bone quality of ISA Brown laying hens. Ninety-six laying hens were assigned to four groups: fed with wheat–corn diet or rye–wheat–corn diet (25% of hybrid rye inclusion) or nonsupplemented or supplemented with xylanase (200 mg/kg of feed) for a period of 25 weeks, from the 26th to the 50th week of age. X-ray absorptiometry, X-ray diffraction, and Fourier-transform infrared spectroscopy were used to provide comprehensive information about the structural organization of bone constitutive phases of the tibia mid-diaphysis in hens from all treatment groups. Bone hydroxyapatite size was not affected by diet. Xylanase supplementation influenced the carbonate-to-phosphate ratio and crystallinity index in hens fed with both diets. Xylanase had more pronounced effects on bone mineral density and collagen maturity in hens fed with the rye–wheat–corn diet versus those fed with the wheat–corn diet. The results of this study showed that modern rye varieties, when supplemented with exogenous xylanase, can be introduced to the diet of laying hens without any adverse effects on bone structure.

Determination of FODMAP contents of common wheat and rye breads and the effects of processing on the final contents

The benefits of a diet, low in fermentable oligo-, di- and monosaccharides and polyols (FODMAP) for patients suffering from irritable bowel syndrome (IBS) has been well established. Thus, the exact knowledge of the amount and composition of FODMAPs in foods is of vital importance for these patients. This study investigated the possibilities of FODMAP reduction by adjusting the processing parameters, which are feasible in practise, while still producing marketable breads. Therefore, the impact of prolonged proofing and the addition of sourdough on the FODMAPs in the final products was evaluated. High performance anion exchange chromatography was used for qualitative and quantitative analysis. A prolonged proofing time resulted in reduction of the fructan content and in consequence of the total FODMAPs. In contrast, the addition of sourdough only altered the FODMAP composition, by reducing the fructan content but increasing the mannitol content. While all the breads produced from refined wheat flour meet the low-FODMAP criteria, the breads from rye and whole meal wheat flour have to be considered as high-FODMAP, regardless of the processing conditions investigated. Breads produced from rye flour exceeded the limits for fructans and mannitol, while whole meal wheat breads were found to exceed the threshold for excess fructose. Overall, the production of low-FODMAP rye breads was identified as the biggest challenge for future research, since it could not be achieved using conventional processing parameter.

Biologically active compounds contained in grape pomace

A healthy lifestyle and gastronomic trends based on traditional and local foods accompanied by waste-free technologies are currently in the primary focus. One of the raw materials with properties in alignment with such requirements is grape pomace. This paper evaluates the antioxidant activity of grape pomace (which is homogenized into a brown powder) and selected commonly available commercial flours – wheat bread, rye plain, and rye whole grain flour – using DPPH (2,2‑diphenyl-1-picryl-hydrazyl-hydrate) and total polyphenol content method, where was used Folin-Ciocalteaure agent and then it was determined by spectrophotometric method (the measure of absorbance). The total amount of polyphenols in grape pomace was measured of 47.94 mg GAE.g-1, but the value 0.27 mg GAE.g-1 was measured in wheat bread flour. Grape pomace performed the antioxidant activity of 57.45 mg AAE.g-1, whereas wheat bread flour of only 0.21 mg AAE.g‑1 Compared to selected commercial flours, the total amount of polyphenols in grape pomace was up to 150 times higher and the ratio of antioxidant activity between grape pomace and wheat bread flour was even more than 280 times higher. This makes it possible to fortify commercial, commonly available flours with different amount of grape pomace so that products with a higher amount of biologically active substances can be prepared. Another benefit could be a reduction in health risks and a contribution to improving consumer health.

Assessments and perspectives on stem rust resistance in South African malting barley

The production of malting barley under dryland and irrigated environments in South Africa (SA) has increased recently, making it the second most important winter crop after wheat. To assess the potential vulnerability of adapted barleys from SA to wheat stem rust (caused by Puccinia graminis f. sp. tritici, Pgt, race PTKST) and rye stem rust (Puccinia graminis f. sp. secalis, Pgs, isolate UVPgs1), 50 accessions were evaluated as seedlings and as adult plants. The same barley germplasm was tested against Pgt and Pgs cultures from the USA that have proven useful for detecting the stem rust resistance genes Rpg1 and the rpg4/Rpg5 complex. The possible presence of these resistance genes was assayed using molecular markers and sequence analysis. Barley accessions exhibited mostly resistant infection types (ITs) to the South African cultures, whilst field data revealed that several of the barley varieties displayed high severities in combination with more compatible host response types to race PTKST. The barley accessions exhibited mostly high ITs to cultures from the USA. None of the seedling IT data was sufficiently distinct to reliably postulate the presence of possible resistance genes. This result was corroborated from the molecular assays for Rpg1 and rpg4/Rpg5. Except for one line that tested heterozygous for the Rpg1 gene, none of the test entries contained this gene or a functional rpg4/Rpg5 complex due to the prevalence of mutations in the rpg4/Rpg5 + carriers. Susceptibility among barley varieties to wheat stem rust race PTKST under field conditions in the present study is of concern.

Scale development and utilization of universal PCR-based and high-throughput KASP markers specific for chromosome arms of rye (Secale cereale L.)

BackgroundRye (Secale cereale L., 2n = 2x = 14, RR), a relative of common wheat, is a large gene resource pool for wheat improvement. Accurate and convenient identification of the rye chromatin in wheat background will facilitate the transfer and utilization of elite genes derived from rye in wheat breeding.ResultsIn the present study, five rye cultivars including Imperial, German White, Jingzhouheimai, Baili and Guyuan were sequenced by specific-locus amplified fragment sequencing (SLAF-seq) to develop large-scale rye-specific markers. Based on SLAF-seq and bioinformatics analyses, a total of 404 universal PCR-based and a whole set of Kompetitive allele-specific PCR (KASP) markers specific for the 14 individual rye chromosome arms were developed and validated. Additionally, two KASP markers specific for 1RS and 2RL were successfully applied in the detection of 1RS translocations in a natural population and 2RL chromosome arms in wheat-rye derived progenies that conferred adult resistance to powdery mildew.ConclusionThe 404 PCR-based markers and 14 KASP markers specific for the 14 individual rye chromosome arms developed in this study can enrich the marker densities for gene mapping and accelerate the utilization of rye-derived genes in wheat improvement. Especially, the KASP markers achieved high-throughput and accurate detection of rye chromatin in wheat background, thus can be efficiently used in marker-assisted selection (MAS). Besides, the strategy of rye-specific PCR-based markers converting into KASP markers was high-efficient and low-cost, which will facilitate the tracing of alien genes, and can also be referred for other wheat relatives.

Effects of Alkalinity and Acidity of the Root Medium on Defense Systems in Triticum aestivum and Secale cereale

The leaves of spring wheat (Triticum aestivum L.) and winter rye (Secale cerealе L.) were used to examine the dynamics of peroxidase activity and the content of hydrogen peroxide, proline, flavonoids, and organic acids (oxalic, succinic, malic, and citric) at early stages of alkaline (pH 10) and acidic (pH 3) stresses imposed by the pH change in the root medium. The results are discussed on the basis of oxidative stress theory and the mechanisms of intracellular pH homeostasis. The dynamics of hydrogen peroxide content and the increased peroxidase activity provided evidence for the enhancement of redox processes. The accumulation of low-molecular-weight polyfunctional compounds, such as proline and flavonoids, indicated the mobilization of plant defense systems. At the same time, stress-related adaptive changes differed qualitatively in different treatments: the leaves of wheat plants transferred to acidic media accumulated proline, while alkaline-treated plants accumulated predominantly flavonoids. The peroxidase activation and proline accumulation after pH changes in the root medium were more strongly pronounced in rye than in wheat plants. A comparatively stable trend to the decrease in the total amount of malic, citric, and succinic acids was observed within 24 h after the onset of stress treatment; in rye leaves, this trend was apparent both with acidic and alkaline root media, while it was evident in wheat leaves under acidic pH of the solution. The observed changes in the content of organic acids that are involved in the Krebs cycle are regarded as a manifestation of the pH-stat mechanism.

Torrefaction of Agricultural Residues: Effect of Temperature and Residence Time on the Process Products Properties

Abstract To date, few studies on the potential utilization of agricultural residue torrefaction products have been performed. Thus, torrefaction product characterization aimed at its potential utilization was performed. Wheat–barley straw pellets and wheat–rye chaff were used in the study. The impact of the torrefaction temperature (280–320 °C) on polycyclic aromatic hydrocarbons (PAHs) content in the biochar and noncondensable gas (noncondensables) composition was investigated. The impact of the torrefaction time (30–75 min) on the composition of the condensable volatiles (condensables) and their toxicity were also studied. The torrefaction process was performed in a batch-scale reactor. The PAH contents were measured using high-performance liquid chromatography (HPLC), and the noncondensables composition was measured online using a gas analyzer and then gas chromatograph with flame ionization detector (GC-FID). The condensables composition and main compound quantification were determined and quantified using gas chromatography–mass spectrometry (GC/MS). Three toxicity tests, for saltwater bacteria (Microtox® bioassay), freshwater crustaceans (Daphtoxkit F magna®), and vascular plants (Lemna sp. growth inhibition test), were performed for the condensables. The PAHs content in the biochar, regardless of the torrefaction temperature, allows them to be used in agriculture. The produced torgas shall be co-combusted with full-caloric fuel because of its low calorific value. Toxic compounds (furans and phenols) were identified in the condensable samples, and regardless of the processing time, the condensables were classified as highly toxic. Therefore, they can be used either as pesticides or as an anaerobic digestion substrate after their detoxification.

Dietary zinc source impacts intestinal morphology and oxidative stress in young broilers

Zinc is an essential nutritional trace element for all forms of life as it plays an important role in numerous biological processes. In poultry, zinc is provided by in-feed supplementation, mainly as zinc oxide or zinc sulfate. Alternatively zinc can be supplemented as organic sources, which are characterized by using an organic ligand that may be an amino acid, peptide, or protein to bind zinc and have a higher bioavailability than inorganic zinc sources. There are limited number of studies directly comparing the effects of inorganic vs. organic zinc sources on performance and intestinal health in broilers. Therefore, a digestibility and a performance study were conducted to evaluate and compare the effect of an amino acid-complexed zinc source vs. an inorganic zinc source on intestinal health. The experiment consisted of 2 treatments: either a zinc amino acid complex or zinc sulfate was added to a wheat–rye based diet at 60 ppm Zn, with 10 replicates (34 broilers per pen) per treatment. Effects on performance, intestinal morphology, microbiota composition, and oxidative stress were measured. Supplementing zinc amino acid complexes improved the zinc digestibility coefficient as compared to supplementation with zinc sulfate. Broilers supplemented with zinc amino acid complexes had a significantly lower feed conversion ratio in the starter phase compared to birds supplemented with zinc sulfate. A significantly higher villus length was observed in broilers supplemented with zinc amino acid complexes at days 10 and 28. Supplementation with zinc amino acid complexes resulted in a decreased abundance of several genera belonging to the phylum of Proteobacteria. Plasma malondialdehyde levels and glutathione peroxidase activity showed an improved oxidative status in broilers supplemented with zinc amino acid complexes. In conclusion, zinc supplied in feed as amino acid complex is more readily absorbed, potentially conferring a protective effect on villus epithelial cells in the starter phase.

The ritual and festive cuisine of the Czechs of the South of Ukraine as a marker of ethnic identification (based on ethnographic research materials of the Zaporozhye region)

The article analyzes features of the ceremonial and festive cuisine of modern descendants of Czech immigrants living in р. Novgorodkovka of Melitopol District of Zaporozhia Region. Ethnographic materials of the author, recorded from Czech respondents in 2000, 2011-2013, became a source for scientific work. For the Czechs, as for most Slavic peoples, cooking was typical: boiled, stewed, fried and baked. Of particular importance in this case acquires the design of the furnace. The main body of the furnace was in the living room. In the summer they also used ovens in the open air. Until the end of the twentieth century, electric ovens entered the peasant life. Folk ritual food (festive, ritual, ceremonial) was not only a wayto meet the vital needs of a person, but became an important form of social intercourse. By comparing results of the author’s field studies and published data on the immigrants’ cuisine in the motherland, it was established that, on the one hand, the folk ritual and festive food has saved its basic elements and methods of preparation. On the other hand, because of close interethnic Czech-Russian-Ukrainian contacts, it has gained new characteristics. In particular, in the memorial kitchen, the rye wheat porridge with raisins was replaced with boiled rice with cherries; baked apples with sugar were replaced with dried butter and ghee. Wedding dishes complemented the new version of baking − the cow, which served as the ceremonial attribute. The food of the calendar cycle of holidays was characterized by the preservation of Czech ethnic traditions. However, there were: Easter ceremonial bread (Pasca), dumplings, sea fish. In after the birth rite, nutritional elements were most important in the visiting the mother and child.

Immunological Factors Associated with Adult Asthma in the Aseer Region, Southwestern Saudi Arabia

Background: The prevalence of asthma is on the rise in Saudi Arabia. Data regarding the immunological profile of asthma in adults in the Aseer region, in southwestern Saudi Arabia, have not been well studied. Objectives: Our aim was to study the immunological factors associated with sensitization to asthma among adults in the Aseer region. Methods: A cross-sectional study with a nested case control design in a 1:1 ratio was conducted on a sample of adults attending primary health care centers in the Aseer region. The study used a validated Arabic version of the International study of asthma and allergies in childhood (ISAAC) questionnaire. The presence of wheezing in the past 12 months was used as a proxy for bronchial asthma. Matched age and sex controls were selected. Both groups were tested for complete blood count (CBC), total and differential white blood cell (WBC) count including eosinophils, total immunoglobulin E (IgE) measurement, allergen-specific immunoglobulin E (IgE), and cytokine levels. Results: The present study included 110 cases and 157 age- and sex-matched controls. Rye wheat was found to be a significant outdoor sensitizing agent ((odds ratio) OR = 5.23, 95% CI: 1.06–25.69). Indoors, house dust mites Dermatophagoides petronyssinus (OR = 2.04, 95% CI: 1.04–3.99) and Dermatophagoides farinae (OR = 2.50, 95% CI: 1.09–5.75) were significant. Higher total IgE (OR = 1.84, 95% CI: 1.10–3.06) and eosinophil levels (OR = 2.85, 95% CI: 1.14–7.15) were significantly associated with adult bronchial asthma in Aseer. On the other hand, the role of cytokines was not significant. Conclusions: In the present study, certain environmental agents were found to be important with regards to sensitization to bronchial asthma in adults. Knowledge about these sensitization agents should be disseminated to health providers and treating physicians in order to enhance preventive environmental control measures and asthma management. Asthma-treating physicians in the region should be alerted to the use of targeted biological therapies in selected asthmatics with difficult-to-control courses.

Effective introgression of wheat D-genome chromosomes into hexaploid triticale (× Triticosecale Wittm.) using trigeneric hybrids

Given the narrow genetic variability of hexaploid triticale, introgressive hybridization is an efficient means to enrich the available genetic pool. The wheat D-genome chromosomes harbor valuable genes for triticale improvement, such as enhancement of biotic and abiotic stress resistance, bread baking quality, and agronomically useful characters. Introducing the wheat D-genome chromosome into triticale may thus improve its agronomic performance and quality. In the present study, we successfully developed the six hexaploid triticale D-genome chromosome introgression lines derived from wheat–rye–Psathyrostachys huashanica trigeneric hybrids. Genomic and fluorescence in situ hybridization (GISH and FISH) karyotyping revealed that K16-4173-3, K16-1565-6, K16-1565-7, and K16-601-1 were 2D(2R), 3D(3B), 3D, 5D(3B, 5R), and 5D(5B) chromosome substitution lines, respectively. K16-4121-3 was found to be a disomic chromosome addition line having a pair of 6D chromosomes. In addition, line K16-1566-4 carried 28 A- and B-genome, 12 R-genome (lacking two 4Rs), two 4D, and two 7D chromosomes. Analysis of these lines using wheat simple sequence repeats (SSRs) and rye specific molecular markers confirmed that corresponding triticale B or R chromosomes had been replaced by D chromosomes of wheat, an observation perfectly consistent with the FISH and GISH results. Furthermore, these lines except for K16-601-1 were highly resistant to the stripe rust pathogen prevalent in China, than Zhongsi828 plants at the adult stages. Our data suggest that the use of trigeneric hybrids as a bridge between triticale and amphiploid wild-wheat forms is an effect strategy for transferring D-genome chromatin into triticale from other breeding programs. The triticale D-genome introgression lines created in this study may serve as new bridging germplasm for hexaploid triticale breeding and should likewise be useful for enhancing triticale genetic diversity.

LUNASIN AND ITS VERSATILE HEALTH-PROMOTING ACTIONS

The present review focuses on the potential health benefits of lunasin reported to date. Epidemiological studies on human and animals have demonstrated lower incidence of various types of cancer and other chronic diseases related to consumption of diets containing high amounts of soybean products. Naturally present in soybean, lunasin is the most isolated bioactive peptide with 43 amino acid residues, some of them creating an RGD motif, chromatic-binding helical region and an aspartic acid tail. Due to the three multiple functional domains, the intriguing peptide serves as promising candidate in exerting many-sided health-beneficial actions, such as antioxidant capacity, anti-inflammatory, hypocholesterolemic, and anticancerous activities. Depending on the soybean genotype, the lunasin content in the crop varies from 0.5 - 8.1 mg/g of seed. Besides soybean, it has also been identified in wheat Solanum nigrum, rye, barley, amaranth seeds, triticale and quinoa. In the current review, the structure, bioavailability, and molecular mechanisms involved in health-promoting effects of lunasin have also been summarized.

Development and Characterization of Wheat-Rye Hybrids Produced by Meiotic Restitution

The nature of the formation of fertile progenies of F2 and F3 generations of wheat–rye hybrids obtained as a result of meiotic restitution using the Saratovskaya 29 variety (C29 x R) and the wheat–rye substituted line 1Rv(1A) (1Rv (1A) × R) was studied. The karyotype analysis of the F2Triticum aestivum L. cv. Saratovskaya 29 × Secale cereale L. var. Onochoyskaya (S29 × R) hybrid using C-banding revealed 56 chromosomes; among them were 42 wheat chromosomes and 14 rye chromosomes. The karyotype of the F2 1Rv(1A) × R hybrid contained 46 chromosomes, of which three pairs of rye chromosomes 1R1R4R4R2RL2RL, 1R1R replaced the chromosomes 1A1A, and 2RL2RL and 4R4R were additional to the wheat chromosomes. In the F3 generation of S29 × R hybrids, the octoploid number of chromosomes with aneuploidy of single rye and wheat chromosomes was preserved, while in the 1Rv(1A) × R hybrids the number of chromosomes varied from 42 to 49, but in most plants 2n = 46 was retained. Meiosis of the F3 1Rv(1A) × R hybrids was more stable than that of the S29 × R hybrids. The average number of univalents in 1Rv(1A) × R hybrids was 1.54 ± 0.17, which was significantly lower than in S29 × R hybrid progenies (2.56 ± 0.23 and 2.07 ± 0.27). The F3 С29 × R and 1Rv(1A) × R hybrids were characterized by the variability in the seed set and 1000-grain weight. On average, С29 × R plants produced a smaller number of grains with but larger size than 1Rv(1A) × R plants, forming more grains with lower 1000-grain weight. The obtained results indicate the influence of the substitution 1Rv(1A) on the reorganization of hybrid genomes.

Identification of novel C-repeat binding factor (CBF) genes in rye (Secale cereale L.) and expression studies

Although rye is one of the most cold-tolerant species among temperate cereals, its huge and complex genome has prevented us from identifying agronomically useful genes. However, advances in high-throughput sequencing technology are making it increasingly possible to investigate its genome. The C-repeat binding factor (CBF) gene family controls cold tolerance in plants and its members are well conserved among eudicots and monocots, among which there are diverse homologs. Despite its large genome, only a small number of CBF genes have been identified in rye. In this study, we explored high-throughput sequencing data of the rye genome and identified 12 novel CBF genes. Sequence analyses revealed that these genes contain signature sequences of the CBF family. Chromosomal localization of the genes by PCR using wheat–rye addition lines showed that most of these are located on the long arm of chromosome 5, but also on the long arm of chromosomes 2 and 6. On the basis of comparative analyses of CBF family members in the Triticeae, CBF proteins were divided into several groups according to phylogenetic relationship and conserved motifs. Light is essential to fully induce CBF gene expression and there is specificity in the response to different types of abiotic stresses in ScCBF genes. The results of our study will assist investigations of CBF genes in the Triticeae and the mechanism of cold tolerance through the CBF-dependent pathway in plants.

Impact of soil press work on crop yield in the forest steppe of Western Siberia

Soil compaction after sowing reduces overall porosity, increases thermal conductivity and heating of the upper layer, which increases the contact of seeds with the soil, accelerates water consumption, swelling and germination of seedlings of crops. Currently, the most part of drills, including seeding systems, press the soil by the rolls of different modifications simultaneously with sowing. After sowing crops, the upper (0-10 cm) soil layer has insufficient compaction (less than 1.00-1.05 g/cm3) and increased non-capillary porosity (more than 50%), which leads to moisture losses from the upper layer. The researchers found out that rolling-up contributes to increasing of the top layer clumpiness on 5.8% on average and reduces erodability of the field surface on14.4% with weak soil response to deflation (less than 50 g). The experimental results on effectiveness of additional pressrolling after sowing SKP-2.1 on the fall variants of the steam field treatment to a depth of 20-22 cm showed that seed germination was increased on 11.3%, and grain increase was 0.09-0.15 t/ha or 4.2-7.2%. On minimal steam treatment, (depth of 10-12 cm) additional soil press rolling was not sufficiently effective. On the black-earth soils of the forest-steppe of Western Siberia, press-rolling of the soil after sowing SKP-2,1 contributes to additional grain yield of winter rye and durum wheat to 0,14-0,23 t/ha (6,1-11,0 %). Additional rolling after sowing by SKP-2,1 and John Deere 1820 with pointed tines for autumn backgrounds increases grain yield in the second wheat after steam from 2.52 to 2.74 t/ha, or 8.7%.

Leguminous pre-crops improved quality of organic winter and spring cereals

ABSTRACTThe aim of this research was to assess the effect of leguminous pre-crop species on quality characteristics of subsequent spring and winter cereals. The experiment was carried out in an organic crop rotation in north-eastern Europe. The influence of biomass dry matter yield, carbon and nitrogen content of perennial red clover (Trifolium pratense L.), alsike clover (Trifolium hybridum L.), Washington lupin (Lupinus polyphyllus Lindl.), biennial white sweet clover (Melilotus albus L.), annual crimson clover (Trifolium incarnatum L.) and Alexandria clover (Trifolium alexandrinum L.) on protein concentration, bulk density and thousand grain weight of subsequent cereals were assessed. Barley, oats, winter rye, spring and winter wheat were grown in the first post-legume year and barley and oats in the second post-legume year. In the first year after perennial and biennial pre-crops, the protein concentration of the cereals, except for rye, increased by 0.8–2.6 percentage points compared with the control. The largest increases in protein concentration of the cereals were after red and alsike clovers. The legumes increased the bulk density of all of the cereals, while thousand kernel weights were increased only for barley, spring and winter wheat. All the legume species had a positive second-year after-effect on the protein concentration of barley and oats, with the largest effect after red and alsike clover at 1.0–1.3 percentage points. The results showed that all of the leguminous pre-crops were suitable for increasing the quality of cereals. The effect was greater after perennial and biennial species compared with the annual species.

Two Rye Genes Responsible for Abnormal Development of Wheat⁻Rye Hybrids Are Linked in the Vicinity of an Evolutionary Translocation on Chromosome 6R.

The post-zygotic reproductive isolation (RI) in plants is frequently based on the negative interaction of the parental genes involved in plant development. Of special interest is the study of such types of interactions in crop plants, because of the importance of distant hybridization in plant breeding. This study is devoted to map rye genes that are incompatible with wheat, determining the development of the shoot apical meristem in wheat–rye hybrids. Linkage analysis of microsatellite loci, as well as genes of embryo lethality (Eml-R1) and hybrid dwarfness (Hdw-R1) was carried out in hybrids of Chinese Spring wheat with recombinant inbred lines as well as interline rye hybrids. Eml-R1 and Hdw-R1 could be mapped proximal and distal of two closely linked EST-SSR markers, Xgrm902 and Xgrm959, on rye chromosome 6R. Both rye genes are located on a segment of chromosome 6R that contains a breakpoint of evolutionary translocation between the ancestral chromosomes of homeologous groups 6 and 3. The obtained results are discussed in relation to genes interacting in developmental pathways as a class of causal genes of RI.

Assessment of dietary exposure to inorganic chloride, phosphate, and sulphate bread additives by ion chromatography with conductometric detection

Bread is one of basic human dietary items. Bread products commercially available usually contain flour, water, yeast or sourdough, and numerous functional additives, among them inorganic chlorides (mainly NaCl), phosphates, and sulphates, modifying physicochemical properties of final products to be attractive for consumers. Various kinds (whole-wheat, rye, and wheat rye) of Polish commercial breads were examined for contents of chlorides, phosphates, and sulphates by combination of water extraction and ion chromatography with conductometric detection. The evaluated amounts of the analytes corresponded to 0.58–1.06 g of chlorides (1–1.8 g NaCl), 100–300 mg of phosphates, and 10–130 mg of sulphates in 100 g (ca. two slices) of bread, which means that bread can be an important source of inorganic ions for humans, in particular in case of high consumption.