Abstract: Soil is a well-studied ecological niche for the discovery of beneficial biologically active chemicals like therapeutically relevant antibiotics. Actinobacteria have been recognised as one of the largest microbial groups in soil that are capable of producing a wide range of beneficial secondary metabolites and compounds with varying characteristics. Therefore, the current study aimed to assess antimicrobial and antioxidant activities of Streptomyces rimosus ACW9 isolated from wheat farm soil. Molecular identification (16S rRNA ribotyping) was used to identify strain ACW9. Ethyl acetate crude extract displayed promising antibacterial activity against clinically isolated uropathogens, Enterobacter sp. (20 mm) and Bacillus subtilis (14 mm). In addition, the extract showed 62.3% DPPH scavenging with IC50= 1.829 mg/ml. The characterization of the purified metabolites from strain ACW9 was done by GCMS, FTIR, and HPLC techniques. The profound metabolites of ethyl acetate extract were identified by GCMS were cyclohexane, (-2-Nitro-2-Propyl), hexadecane, and pentanoic acid. It may be concluded that actinobacteria isolated from wheat farm soil is a promising source for medicinally important bioactive compounds
Read full abstract