Wheat Extracts Research Articles

Chemical, rheological and sensory characteristics of wheat bread enriched with chia (Salvia hispanica L.) seed gum

Bread is a staple food in many countries worldwide. The aim of this study is the presentation of a novel type of bread prepared of wheat flour and extract of chia seed gum (CSG). The CSG was included in the wheat flour mixture in a concentration from 2 to 10%. The flour mixture was characterized by its rheological properties through amylography, farinography, and extensography tests. The bread was also subjected to texture, color, and tactile analysis. The highest grades of the bread in terms of appearance, flavor, aroma, texture, and color were shown for flour mixture that contained 10% of CSG (p < 0.05); namely, this wheat mixture showed an improved value of volume, specific size, external and internal characteristics of bread.

Effect of chitosan and natural materials on properties of polyvinylchloride for blood bags applications

Abstract Polyvinyl chloride is the most common material used in the manufacture of blood bags, and chitosan and wheat extract were used to improve the biological, physical, mechanical and optical properties of the manufactured blood bag. Chitosan was added at three concentrations (1, 2, and 3%) to the PVC matrix sample, and the wheat extract was supplemented to the sample having the highest properties to study its effect. It was found that the addition of chitosan led to an increase in tensile strength, as well as a significant increase in inhibition against the bacterial activity of two types of bacteria (S. aureus and C. albicans), and the ability to absorb water, but the addition of wheat extract led to a very small decrease in absorbency. Also, the supplement of chitosan and wheat extract reduced the transparency of the manufactured blood bag. All samples were characterized as not having the ability to non-hemolytic in the hemolysis test. All the results of polymer blends and polymer composite were compared with the pure PVC and commercial blood bags as reference samples.

Estimation the Properties of Polymer Matrix Reinforced with Plants Extract Used for Medical Fluid Bags

Abstract The utilize of polyvinyl chloride in the manufacturing of blood bags responsible for preserving blood. It attains an extensive range of performance and processing requirements such as flexibility, transparency, physical, antibacterial and non-hemolysis properties. So, in the present flax-seed gel was incorporated in PVC matrix at various concentration (1, 2 and 3) %. Then wheat extract was added to samples has better properties in two percentage (0.5 and 1) %. The obtained results show, a significant change in the properties of the composite produce, where the tensile strength increased with increasing concentration of extract from flax seed gel, but it decreased when wheat extract was added, and when both extracts were added, the inhibition zone increased against bacterial activity for two types of bacteria (S. aureus and C. albicans). Also, the ability to absorb water and wettability increased with increasing concentration of plant extracts. On the other hand, the transparency decreased with increasing concentration of flaxseed gel, but when wheat gel was added to it increase of transparency. All samples were non-hemolytic in hemolysis test.

Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform.

Climate change poses a significant threat to global agriculture, necessitating innovative solutions. Plant synthetic biology, particularly chloroplast engineering, holds promise as a viable approach to this challenge. Chloroplasts present a variety of advantageous traits for genetic engineering, but the development of genetic tools and genetic part characterization in these organelles is hindered by the lengthy time scales required to generate transplastomic organisms. To address these challenges, we have established a versatile protocol for generating highly active chloroplast-based cell-free gene expression (CFE) systems derived from a diverse range of plant species, including wheat (monocot), spinach, and poplar trees (dicots). We show that these systems work with conventionally used T7 RNA polymerase as well as the endogenous chloroplast polymerases, allowing for detailed characterization and prototyping of regulatory sequences at both transcription and translation levels. To demonstrate the platform for characterization of promoters and 5' and 3' untranslated regions (UTRs) in higher plant chloroplast gene expression, we analyze a collection of 23 5'UTRs, 10 3'UTRs, and 6 chloroplast promoters, assessed their expression in spinach and wheat extracts, and found consistency in expression patterns, suggesting cross-species compatibility. Looking forward, our chloroplast CFE systems open new avenues for plant synthetic biology, offering prototyping tools for both understanding gene expression and developing engineered plants, which could help meet the demands of a changing global climate.

A disposable immunosensor array using cellulose paper assembled chemiresistive biosensor for simultaneous monitoring of mycotoxins AFB1 and FB1

Due to the common contamination of multiple mycotoxins in food, which results in stronger toxicity, it is particularly important to simultaneously test for various mycotoxins for the protection of human health. In this study, a disposable immunosensor array with low-cost was designed and fabricated using cellulose paper, polydimethylsiloxane (PDMS), and semiconducting single-walled carbon nanotubes (s-SWCNTs), which was modified with specific antibodies for mycotoxins AFB1 and FB1 detection. The strategy for fabricating the immunosensor array with two individual channels involved a two-step protocol starting with the form of two kinds of carbon films by depositing single-wall carbon nanotubes (SWCNTs) and s-SWCNTs on the cellulose paper as the conductive wire and sensing element, followed by the assembly of chemiresistive biosensor with SWCNTs strip as the wire and s-SWCNTs as the sensing element. After immobilizing AFB1-bovine serum albumin (AFB1-BSA) and FB1-bovine serum albumin (FB1-BSA) separately on the different sensing regions, the formation of mycotoxin-BSA-antibody immunocomplexes transfers to electrochemical signal, which would change with the different concentrations of free mycotoxins. Under optimal conditions, the immunosensor array achieved a limit of detection (LOD) of 0.46 pg/mL for AFB1 and 0.34 pg/mL for FB1 within a wide dynamic range from 1 pg/mL to 20 ng/mL. Furthermore, the AFB1 and FB1 spiked in the ground corn and wheat extracts were detected with satisfactory recoveries, demonstrating the excellent practicality of this established method for simultaneous detection of mycotoxins.

Селективные сорбенты для микотоксинов на основе импринтированных структур полианилина

The constant increase in contamination of agricultural products with mycotoxins makes it urgent to develop new methods for their sorption, isolation, and determination. Molecular imprinting is a promising method for recognizing and isolating target molecules based on specific sorption mechanism. The work considers the possibility of obtaining and practical use of mycotoxin–specific selective sorbents based on imprinted polyaniline structures using a structural analogue of the mycotoxin zearalenone – 4-hydroxycoumarin. The optimal carrier for the synthesis of molecularly imprinted polymers has been selected. The specificity of 4-hydroxycoumarin sorption from model solutions has been studied. The practical possibility of sorption and extraction of ZEA from an artificially contaminated wheat extract has been demonstrated.

Effect of gastrointestinal digestion on the stability, antioxidant activity, and Caco-2 cellular transport of pigmented grain polyphenols.

Grain polyphenols are known to possess several health properties. However, their digestive stability and intestinal absorption have not been fully elucidated. This study investigated the fate of pigmented grain polyphenols in the digestive system. Purple rice, purple barley, purple wheat, and blue wheat extracts were subjected to simulated gastric and intestinal phase digestion, followed by Caco-2 cellular transport. Phenolic profiling and antioxidant activity were determined using benchtop assays and an ultra-high-performance liquid chromatography-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid free radical) (UHPLC-ABTS•+) system. The results demonstrated a decrease in the total phenolic content of extracts after digestion, with purple rice extract retaining the highest phenolic content (79%) and ABTS•+antioxidant activity (31%). Antioxidant activity was retained the most during the gastric phase; however, dominant antioxidant compounds were not detected after intestinal digestion. Significant variations in phenolic composition and radical scavenging activity were detected after digestion. Protocatechuic acid, vanillic acid, apigenin, and chrysoeriol were all transported across the intestinal barrier. The findings of this study provide novel insights into the in vitro stability and antioxidant activity of cereal grain polyphenols after simulated digestion.

Simultaneous determination of total ergot alkaloids in wheat flour by Orbitrap mass spectrometry

The optimization screening methods for total ergot alkaloids in wheat extracts involve transforming them into a single compound, which is then analyzed via high-resolution Orbitrap mass spectrometry (Orbitrap MS). Orbitrap MS provides highly sensitive and accurate mass measurements, enhancing the selectivity and sensitivity of the analysis. Various hydrolysis and reduction methods have been investigated, and the use of superhydrides has emerged as the most effective method for transforming ergopeptine alkaloids. This study also focused on the epimerization of ergot alkaloids, particularly the differences between R- and S-epimers and their impact on the mass spectra. We validated our method by assessing the linearity, sensitivity, recovery, matrix effects, repeatability, and stability. The limits of detection and quantitation were set at 0.43 and 1.30 μg LSA/kg wheat, respectively. The proposed method offers a robust analytical approach for screening and quantifying total ergot alkaloids in wheat samples, addressing important concerns about their presence in food and feed.

Efficiency of some inhibitors on alpha amylase of the peach fruit fly larvae Bactrocera zonata (Saunders) (Diptera: Tephritidae)

Alpha amylase inhibitor (α-AI) extracted from wheat grains (Triticum sp.) as possible new agent in pest control, and some other organic inhibitors are evaluated against α-amylase of the fourth larval instar of the peach fruit fly (PFF) larvae; Bactrocera zonata (Saund.). 4.2 mg protein ml−1 of the inhibitor extracted by 70 % ammonium sulphate inhibited, in vitro, 97.9 % of amylolytic activity of the larvae. Percent inhibition efficiency (Ei%) of the crude inhibitor was directly proportional with its concentration in the range from 4.2 to 0.21 mg protein ml-1. The calculated IC50 was 1 mg protein ml-1. Increasing of the surrounding temperature from 10 to 35 °C not enhanced inhibitor efficiency, and the inhibitor could inhibit the enzyme efficiently at a relatively low temperature (10 °C). On the other hand, wheat extract showed quick binding affinity to α-amylase of PFF larvae. Incubation of the inhibitor with the enzyme for 10 min was sufficient for wheat α- AI to exert its maximum inhibition. Screening of other possible organic compounds act as α-AI such as citric, gallic and acetic acids showed that gallic acid was the most potent inhibitor, and it was able to inhibit α-amylase of the larvae, at a relatively, low concentration (10−4M). It could be concluded that there are natural potent inhibitors of PFF α-amylase which act as a key enzyme for carbohydrates digestion. This might be useful as an alternative method for controlling B. zonata population. Additional experiments are necessary to make these alternative compounds applicable in the field.

Omega-5-gliadin-specific immunoglobulin E-positive, but wheat-specific immunoglobulin E-negative wheat allergy dependent on augmentation factors—a frequent presentation

Aim: Most patients with wheat allergy dependent on augmentation factors (WALDA) show specific immunoglobulin E (sIgE) to ω5-gliadin. However, some WALDA patients may show negative results when testing for sIgE to total wheat extract. This is the first study to investigate potential clinical and serological differences in patients with ω5-gliadin-positive, challenge-confirmed WALDA dependent on their sensitization to total wheat extract. Methods: Clinical and serological characteristics of patients with challenge-confirmed, ω5-gliadin-positive WALDA were analyzed based on the absence or presence of sIgE to wheat (cut-off 0.35 kUA/L). Results: Thirty-six patients with challenge-confirmed WALDA were included (19 female; median age 50.5 years; median sIgE to ω5-gliadin 6.5 kUA/L). SIgE levels to grass pollen were related to the presence of any atopic comorbidity (P &lt; 0.001) and showed a correlation with sIgE to wheat (P = 0.003), but not to the gluten-related allergens [all not significant (ns)]. Thirty-nine percent of patients (n = 14) showed sIgE levels to wheat lower than 0.35 kUA/L; in 19.4% (n = 7) levels were even below the detection limit of 0.01 kUA/L. WALDA patients without sIgE to wheat showed lower levels of total immunoglobulin E (IgE) and sIgE to wheat gluten, gliadins, and ω5-gliadin (all P &lt; 0.001) as well as to grass pollen (P = 0.03). No significant differences in clinical characteristics like delay until diagnosis, the presence of an atopic condition, reaction severity, or threshold in the oral challenge test were observed. Conclusions: SIgE to wheat extract was associated not only with sensitization against gluten allergens but also reflected total IgE production and concomitant grass pollen allergy, making it an insensitive and unspecific biomarker for WALDA. There were no clinical divergences between WALDA patients without or with sIgE to wheat. SIgE to total wheat extract does not appear to be clinically relevant and remains negative in a significant proportion of WALDA patients.

Potential Biopesticides from Seed Extracts: A Sustainable Way to Protect Cotton Crops from Bollworm Damage

In the current study, the inhibitory effect of extracts from Gramineae (wheat, barley, and corn) and Leguminosae (sophora, bean, and pea) seeds was studied on the digestive alpha-amylase activity in cotton bollworm (Helicoverpa armigera Hubner). The insect was bred on artificial food based on Vigna unguiculata in the greenhouse condition (26 ± 2 °C, 60 ± 10% (Relative Humidity), R.H., 16:8 (Light: Darkness), L: D). The extracts of wheat (95.2%), barley (84.6%), corn (73.8%), sophora (77%), Vigna unguiculata (52%), and pea (56.7%) significantly inhibited the alpha-amylase activity in H. armigera. Studying the impact of different fractions (obtained via deposition at various concentrations of ammonium sulfate salt) on the alpha-amylase enzyme activity demonstrated that in 0–30% fractions, wheat, barley, and sophora have the highest effect (95.26%, 94.65%, and 94.73%, respectively) compared to the other fractions. The inhibitory activities of 0–30% fractions of corn, bean, and pea were 83.3, 56.94, and 50.92%, respectively. In 30–50% fractions, the most effective ones were those of wheat and barley with the inhibitory activity of 79.7% and 82.9%, respectively. In addition, bean and pea fractions inhibited 25.2% and 27.5%, in that order. No significant inhibitory impact was detected in 50–70% or higher fractions. The investigation of the impact of pH values (i.e., 2, 4, 8, and 10) on the inhibition of the alpha-amylase enzyme activity introduced 8–10 as the optimum pH in H. armigera. Nanotechnology offers several ways to enhance plant-based pesticides, which are a solution for making plant extract usage more efficient. The exploration of plant-based pesticides, in conjunction with the incorporation of nanotechnology and other scientific fields, offers a wide range of prospects for further investigation.

Identification and Categorization of Yellow Rust Infection in Wheat through Deep Learning Techniques

The global wheat industry faces significant challenges due to yellow rust disease, This is induced by fungus Puccinia striiformis, as it leads to substantial crop losses and economic impacts. Timely detection and classification of the disease are essential for its effective management and control. In this study, we investigate the potential of DL and ML techniques for detecting and classifying yellow rust disease in wheat. We utilize three state-of-the-art CNN models, namely ResNet50, DenseNet121, and VGG19, to analyze wheat leaf images and extract relevant features. These models were developed and refined using a large dataset of annotated wheat photos. Encompassing both healthy plants and those affected by yellow rust disease. Furthermore, we examine the effectiveness of data augmentation and transfer learning in enhancing classification performance. Our findings reveal that the DL-based CNN models surpass traditional machine learning techniques in detecting and classifying yellow rust disease in wheat. Among the tested CNN models, EfficientNetB3 demonstrates the best performance, emphasizing its suitability for large-scale and real-time monitoring of wheat crops. This research contributes to the development of precision agriculture tools, laying the groundwork for prompt intervention and management of yellow rust disease, ultimately minimizing yield loss and economic impact on wheat production.

The Impact of Distillers Dried Yeast on the Production Performance and Fecal Microbiome of Broiler Chickens

The aim of this research was to assess the influence of partially substituting post-extraction soybean meal with distillers dried yeast on the efficiency of broiler chicken fattening and the intestinal microflora.I Inactive distillers dried yeast Saccharomyces cerevisiae, cultivated on wheat extract, was employed in the study. The research was conducted on 112 Ross 308 line cockerels subjected to a 35-day fattening period, during which they were fed Starter (days 1–10), Grower (days 11–27), and Finisher (days 28–35) diets. Broiler chickens were randomly divided into four groups: control and three experimental groups with varying levels of distillers dried yeast—3%, 6%, and 9%. Cockerels in the control group (Starter diet) exhibited higher feed consumption compared to those receiving 3% yeast in their diet. Cockerels in the group receiving 3% yeast (Grower diet) demonstrated better feed conversion compared to those receiving 9% yeast in their diet. The study revealed that regardless of the broiler chicken fattening period, there was a decrease in the overall number of isolated microorganisms in the feces with an increase in the proportion of distillers dried yeast in the diet. This was confirmed by the total number of bacteria, anaerobic bacteria, Enterobacteriaceae, E. coli, Enterococcus sp., and S. aureus. Based on cluster analysis (dendrogram), it was observed that only a 9% addition of yeast to the diet significantly influenced the quantitative and qualitative composition of the fecal microbiota of broiler chickens. In summary, a properly balanced feed with distillery yeast can be used as a good source of energy and protein in feed mixtures for broilers, serving as a cost-effective alternative to soybean meal. However, for optimal production results and feed costs, the inclusion of yeast in the feed should not exceed 3%.

Valorizing Shrimp Shell as a Source of Chitin with Crude Enzymes from Germinated Winter Wheat and Buckwheat

Chitin is a valuable resource found in shrimp shells. Typically, the industrial process for isolating chitin from crustacean shells involves using chemical methods that require large amounts of acid and alkaline. While commercial protease application is a more environmentally friendly method, it can be costly. Germinated grains can be a source of low-cost protease for chitin isolation. This study aimed to assess the effectiveness of crude enzymes extracted from germinated winter wheat (Triticum aestivum) and buckwheat (Fagopyrum esculentum) to isolate crude chitin from white shrimp shells. The proteolytic and chitinolytic activities of the crude enzyme extracts were determined and applied in the deproteinization step during chitin isolation from shrimp shells. The deproteinization rate was assessed, the crude chitin yield was compared, and the isolated chitins were characterized through Fourier Transform Infrared (FTIR) spectroscopy analysis and x-ray diffraction (XRD) analysis. Crude enzymes extracted from germinated winter wheat and buckwheat exhibited proteolytic activity of approximately 0.49 U/mL and 0.46 U/mL, respectively. Using winter wheat extract and buckwheat extract acted on demineralized shrimp shells exerted a deproteinization rate of 53.6 ± 1.0% and 58.0 ± 2.3% and yielded 30.3 ± 5.31% and 29.2 ± 3.99% crude chitin, respectively. FTIR spectra of the isolated chitins showed amide I, amide II, amide III, asymmetric stretching of the C-O-C bridge, O-H stretching, N-H stretching and asymmetric C-H stretching, typical functional groups for chitin. The crystallinity index for commercial chitin, chemical-isolated chitin, winter wheat extract-isolated chitin and buckwheat extract-isolated chitin was 86.49%, 88.74%, 88.82% and 75.87%, respectively. Buckwheat extract-isolated chitin, deacetylated with lower crystallinity, warrants further investigation. Crude enzymes from winter wheat and buckwheat to remove protein from demineralized shrimp shells can be a more environmentally friendly method to valorize shrimp shells as a chitin source.

Effects of the Complex of Panicum miliaceum Extract and Triticum aestivum Extract on Hair Condition.

Proso millet (Panicum miliaceum L.) and common wheat (Triticum aestivum L.) have been used as major crops in multiple regions since ancient times, and they contain various nutrients that can affect human hair health. This study investigated the various biological effects of a complex of millet extract and wheat extract (MWC) on hair health. Human immortalized dermal papilla cells (iDPCs) for an in vitro study and an anagen-synchronized mouse model for an in vivo study were employed. These findings revealed that the application of the MWC in vitro led to an increase in the mRNA levels of antioxidant enzymes (catalase and SOD1), growth factors (IGF-1, VEGF, and FGF7), and factors related to hair growth (wnt10b, β-catenin) while decreasing inflammatory cytokine mRNA levels (IL-6 and TNFα). The mRNA levels of hair follicles (HFs) in the dorsal skin of the mouse model in the early and late telogen phases were also measured. The mRNA levels in the in vivo study showed a similar alteration tendency as in the in vitro study in the early and late telogen phases. In this model, MWC treatment elongated the anagen phase of the hair cycle. These findings indicate that the MWC can suppress oxidative stress and inflammation and may elongate the anagen phase by enhancing the growth factors involved in the wnt10b/β-catenin signaling pathway. This study suggests that the MWC might have significant potential as a functional food for maintaining hair health.

Understanding the impact of water-extractable cereal flour constituents on network formation in gluten-starch dough

Water-extractable cereal flour constituents influence bread loaf specific volume, suggesting a contribution to gas cell (de)stabilization in dough. However, the underlying mechanism remains largely unexplored. We studied the impact of incorporating five cereal flour aqueous extracts in model gluten-starch bread (doughs) on specific volume, dough extensional rheology, and protein extractability changes during bread making. Incorporating wheat, rye, or triticale flour aqueous extracts in gluten-starch doughs increased bread specific volume by 12–17%. There were no changes in dough rheology that could explain this specific volume increase, suggesting that other mechanisms relating to gas cell stabilization might be at play. Incorporating oat flour aqueous extracts decreased bread specific volume by 50%. This negative impact was related to poor dough strain hardening behavior, in turn probably explained by disruption of intermolecular glutenin disulfide bonds, as revealed by chromatography. While this study has provided a novel perspective on the mechanisms by which water-extractable cereal flour constituents influence bread volume, it also demonstrates that the compositional heterogeneity of the flour aqueous extracts needs to be addressed to allow an even better understanding of the role of water-extractable cereal flour constituents in stabilizing gas cells in bread dough.

Treatment of Difficult Lesions with Rigenase® and Polyhexanide: Case Series

The present study aims to evaluate the efficacy and tolerability of Rigenase® and polyhexanide in the treatment of difficult lesions at risk of infection. Rigenase® is a specific wheat extract with antioxidant properties that promote tissue regeneration. Polyhexanide is a latest generation antiseptic that does not give antimicrobial resistance either in vitro or in vivo.

Silver ion chromatography enables the separation of 2-methylalkylresorcinols from alkylresorcinols.

Alkylresorcinols (∑ARs) is the generic term for a highly varied class of lipids found mainly in cereals. These bioactive compounds consist mainly of 5-alkylresorcinols (ARs), which differ in length, unsaturation, and substituents on the alkyl side chain on C-5. In addition, 2-methyl-5-alkylresorcinols (mARs) are scarcely studied minor compounds that are supposed to exist with the same structural diversity. In the first step, ∑ARswere enriched by solid-phase extraction from wheat grain and quinoa seed extracts. The subsequent application of silver ion chromatography (SIC), silica gel, coated with 20% AgNO3 , then deactivated with 1% water) enabled an unprecedented full separation of saturated mARs from conventional ARs. Specifically, saturated mARs were eluted with n-hexane/ethyl acetate (92:8, v/v), and conventional ARs with n-hexane/ethyl acetate (80:20, v/v). The unpreceded separation indicated that the SIC method could be useful not only for separations according to the degree of unsaturation, but also in the case of steric hindrance by additional (alkyl) substituents. Continued fractionation enabled the collection of unsaturated ARs in wheat and quinoa extracts. In this way, 35 ∑ARs (including five mARs) were detected by gas chromatography/mass spectrometry analysis in wheat and 45 ∑ARs (including 21 mARs) in quinoa. These included several low abundant and partly unknown ∑ARs such as 1,3-dihydroxy-5-tricosadienylbenzene.

Activated carbon@silver nanoparticles conjugates as SERS substrate for capturing malathion analyte molecules for SERS detection

AbstractMalathion is one of the commonly used organophosphate pesticides known to attack the central nervous system, posing a risk to humans and other animals upon exposure. The surface enhanced Raman spectroscopy (SERS) has been identified as an indispensable tool for chemical and biomolecular sensing. In this work, the facile fabrication of activated carbon (AC)‐based colloidal SERS active substrate dubbed AC@AgNPs was designed by trapping AgNPs on the surface of AC for detection of varying concentrations of malathion. Apart from the higher concentrations of malathion, the rest of the normal Raman spectra of malathion standard solutions exhibited weak Raman signals. The intensity of peaks for 0.47 mg L−1 were nearly non‐existent which is an indication that the malathion pesticide could only be detected up to 0.95 mg L−1 when using silica wafer. On the contrary, all the SERS spectra of malathion in wheat extracts adsorbed on AC@AgNPs substrate exhibited strong Raman signals. Quantitative analysis of malathion was performed by regression models developed using PLSR built with the raw spectra (no pretreatment), SNV‐PLSR, and SNV‐CARS‐PLSR. The model with the most remarkable performance was established by using SNV‐PLSR with r = 0.9869 and RPD = 4.61. This research shows that the proposed method can rapidly detect malathion residues in wheat, suggesting that it could be adopted for production process monitoring of other related food products to guarantee their safety for human and animal consumption.

Black Wheat Extracts (Arriheuk) Regulate Adipogenesis and Lipolysis via Adenosine Monophosphate (AMP) Activated Protein Kinase (AMPK)/Sirtuin 1 (SIRT1) Signaling Pathways.

Polyphenols and other compounds with antioxidant properties are found in plants and are one of the main antioxidants proven to reduce body weight and the risk of insulin resistance. Still, the mechanism behind the protective effects against obesity remains unclear. Thus, the study aims to assess the impact of flavonoid-rich arriheuk extract, a purple wheat extract, on mitochondrial function using 3T3-L1 adipocytes and investigate the molecular mechanism behind its protective effects against adipogenesis and lipolysis. The study findings strongly indicate that arriheuk significantly suppressed triglyceride levels and inhibited the expression of transcription factors like C/EBPα and PPARγ in 3T3-L1 adipocytes. Furthermore, treatment with arriheuk suppressed the expression of SREBP1c and FAS proteins linked to lipogenesis. In addition, treatment with arriheuk extract decreased the mRNA levels of adipogenic transcription factors, increased glycerol release, and inhibited adipocyte differentiation. Interestingly, the arriheuk-mediated PGC-1α expression triggered mitochondrial biogenesis by promoting the AMPK phosphorylation and SIRT1 expression in adipocytes. Also, arriheuk suppressed adipogenesis and elicited browning through the AMPK- and SIRT1-associated pathways. Collectively, these findings strongly suggest that arriheuk extract regulates browning in 3T3-L1 white adipocytes by triggering the AMPK/SIRT1 pathway, indicating the prospective applications of arriheuk as a functional food to control obesity.