Brazilian Wetlands Research Articles

Automated detection of regions with persistently enhanced methane concentrations using Sentinel-5 Precursor satellite data

Abstract. Methane (CH4) is an important anthropogenic greenhouse gas, and its rising concentration in the atmosphere contributes significantly to global warming. A comparatively small number of highly emitting persistent methane sources are responsible for a large share of global methane emissions. The identification and quantification of these sources, which often show large uncertainties regarding their emissions or locations, are important to support mitigating climate change. Daily global column-averaged dry air mole fractions of atmospheric methane (XCH4) are retrieved from radiance measurements of the TROPOspheric Monitoring Instrument (TROPOMI) on board on the Sentinel-5 Precursor (S5P) satellite with a moderately high spatial resolution, enabling the detection and quantification of localized methane sources. We developed a fully automated algorithm to detect regions with persistent methane enhancement and to quantify their emissions using a monthly TROPOMI XCH4 dataset from the years 2018–2021. We detect 217 potential persistent source regions (PPSRs), which account for approximately 20 % of the total bottom-up emissions. By comparing the PPSRs in a spatial analysis with anthropogenic and natural emission databases, we conclude that 7.8 % of the detected source regions are dominated by coal, 7.8 % by oil and gas, 30.4 % by other anthropogenic sources like landfills or agriculture, 7.3 % by wetlands, and 46.5 % by unknown sources. Many of the identified PPSRs are in well-known source regions, like the Permian Basin in the USA, which is a large production area for oil and gas; the Bowen Basin coal mining area in Australia; or the Pantanal Wetlands in Brazil. We perform a detailed analysis of the PPSRs with the 10 highest emission estimates, including the Sudd Wetland in South Sudan, an oil- and gas-dominated area on the west coast in Turkmenistan, and one of the largest coal production areas in the world, the Kuznetsk Basin in Russia. The calculated emission estimates of these source regions are in agreement within the uncertainties in results from other studies but are in most of the cases higher than the emissions reported by emission databases. We demonstrate that our algorithm is able to automatically detect and quantify persistent localized methane sources of different source type and shape, including larger-scale enhancements such as wetlands or extensive oil- and gas-production basins.

Dietary habits of the giant armadillo (Priodontes maximus) in the Brazilian wetlands

Dietary habits of the giant armadillo (Priodontes maximus) in the Brazilian wetlands

Unraveling the biodiversity in the Brazilian wetlands: dung beetle assemblage in different vegetation types in the Araguaia Pantanal

ABSTRACT The Araguaia Pantanal is characterized by a mosaic of vegetation with high biodiversity. However, our understanding of this biodiversity remains limited and understudied. Therefore, the objective of this study was to evaluate the distribution patterns of dung beetles in different vegetation types (Deciduous Forest, Savanna, Riparian Forest, Murundu Fields, and Exotic Pastures). In addition, we analyzed how forest cover influence the species richness, abundance, and species composition of dung beetles. A total of 12,746 dung beetles representing 67 different species were captured and examined. Our findings revealed that the Murundu Fields exhibited lower species richness and abundance of dung beetles, indicating the influence of distinct vegetation types directly associated with flood pulses in the region. Furthermore, the species composition of the Murundu Fields was found to be a subset of the adjacent vegetation, primarily composed of Savanna and Deciduous Forest species. Additionally, our results demonstrated that tree cover played a significant role in driving dung beetle species turnover among the studied sites, highlighting the species’ response to the existing gradient of vegetational changes within the region. We believe that our study contributes valuable insights into the distribution and ecological dynamics of dung beetles in the Araguaia Pantanal.

SMALL WETLANDS IN THE BRAZILIAN SAVANNA(CERRADO): AN UNDERESTIMATED FRESHWATER RESOURCE

In Brazil, in scientific research and public policy have largely ignored small wetlands, they are not specifically covered by current environmental laws and reference standards. Furthermore, the lack of information about, small wetlands has made their protection and management difficult. Large-scale land use changes that modify the hydrology of low-order streams often lead to the accelerated degradation and loss of small wetlands. The aim of this study conducted in Brazilian Cerrado Biome area, was to map the type, size, and distribution of small wetlands in ancatchment area). Areas represented in 12 high-resolution multispectral images were individually classified in a geographic object-based image analysis (GEOBIA), resulting in a sensitivity or recall 0.872 (~87%). The 7,132 mapped wetlands had a total area of 163km2, corresponding to a density of 4.27wetlands km−2, with smallest wetlands smaller than 4.0 hectares (90.5%). The following descriptors were applied to map small wetlands: feature: asymmetry, rectangular fit, mean nir band, compactness, density, and length. Seven types of natural wetlands and four types of man-made wetlands were accordingly identified in the study area. Their classification within a system that follows the hierarchy of the Brazilian wetland classification system is proposed. Recommendations for the future study and protection of wetlands was are also provided.

Morphological-anatomical and chemical features of Copernicia alba fruits and seeds, a palm from Brazilian Pantanal.

Copernicia alba (Arecaceae) is a palm tree regionally known as carandá that forms large populations and produces abundant fruits, an important food source for the local fauna in Brazilian wetlands. The fruits present morphological variations regarding color, shape and dimensions. In this study fruits of different shapes were collected and processed following routine techniques in plant morphology, and biochemistry analysis of endosperm. (hemicellulose) The fruits isdark, of the berry type, with partially fibrous pericarp, rich in phenolic compounds; the ruminated seed coat also contains phenols; the endosperm, formed of cells with highly thickened, not lignified hemicellulosic walls, stores xyloses, proteins and lipids. The embryo is short and straight. Xylose is the leading sugar of xylan, which can be liberated by hydrolysis with specific enzymes, such as xylanases. This sugar is of interest in several industrial sectors, such as the production of biofuels and xylitol for foods. Excepting depth of seed rumination, C. alba fruits do not have relevant differences in anatomy and classes of substances detected. The fruit yield showed differences associated with its shape, indicating the best utilization. Considering fruit anatomical features and tissue composition, we highlight that the seeds of C. alba have the potential as a new functional food source.

Mineralogy, elemental composition, and organic matter prediction of wetland soils in the Cerrado biome, Brazil

Context The Cerrado, an area of savannah and wetlands in Brazil, is an important wet ecosystem characterised by organic-rich soils that has important environmental functions. In this work, we hypothesised that the total elemental composition obtained via portable X-ray fluorescence spectrometry (pXRF) could be useful a tool to predict soil organic matter. Aims We aimed to predict the total organic carbon (TOC) content and its fractions (carbon fulvic acid, C-FA; carbon humic acid, C-HA; carbon humin, C-HU) of soils occurring in representative Veredas biome from the Cerrado, Brazil. Methods We collected 144 soil samples from surface (0–20 cm) and subsurface (40–70 cm) layers from six representative Veredas environment located in the Cerrado bome, Triângulo Mineiro region, Brazil. These samples were analysed using a pXRF to obtain the total elemental composition. TOC, C-HA, C-FA, and C-HU fractions were determined by wet-oxidation and titration methods. The pXRF data was correlated with TOC and humic substances and later subjected to simple and multiple linear regression (MLR). Key results The major elements corroborated the mineralogy of wetland soils. TOC and humic substances, mainly C-HU, were reasonably predicted using pXRF data. All humic substances and TOC were strongly correlated to Pb. Thus, by parsimony, TOC could be reasonable predicted exclusively based on Pb concentration. Conclusions We demonstrated that pXRF can be an efficient tool to assess the elemental composition of the Cerrado wetlands soils contributing to still scarce inventory. Further, the elements germane to soil organic matter compounds can be used to predict TOC and its fractions. Implications A detailed characterisation of the Cerrado wetland soils in situ can be successfully performed using pXRF in further studies.

Genotoxic risk assessment of solar UV radiation in tadpoles from Brazilian wetlands

Solar ultraviolet (UV) radiation is an environmental genotoxic factor linked to amphibian decline. Here we assessed the genotoxic risk of UVB and UVA exposure for tadpoles from open ponds in southern Brazil, a mid-latitude region influenced by stratospheric ozone depletion. Daily UV doses were measured on the surface of a pond in Taim Ecological Station (TAIM; 32°49’24’’S; 52°38’31’’W) on a cloudless summer day to predict the worst-case scenario for UV-induced DNA damage. Pond descriptors were related to the use of microhabitats by Boana pulchella tadpoles in two ponds over the climate seasons of 2013 and 2014. Our results indicate that shaded microhabitats were more frequent than unshaded ones in autumn, winter, and spring but not in summer. Hence, the penetration of UV radiation into the water of unshaded microhabitats was evaluated through laboratory experiments with artificial UV sources and pond water samples. Physical and biological sensors were applied in the experiments to measure the incident UV radiation and its genotoxic action. By integrating field and laboratory data, we demonstrate that low doses of biologically effective UV radiation reached the tadpoles in autumn, winter, spring, and early summer due to a high proportion of shaded microhabitats and a high concentration of solids in unshaded microhabitats. However, the relative reduction of shaded microhabitats jointly with a declining water level in late summer may have exposed tadpoles to high UV doses. Our experiments also indicate that solar UVB radiation, but not UVA, is primarily responsible for the induction of DNA pyrimidine dimers in organisms living under the surface of aquatic ecosystems. The present work highlights the determinant role of wetland descriptors for minimizing the genotoxic potential of UV radiation and its consequences for amphibians.

Human pressure drives biodiversity-multifunctionality relationships in large Neotropical wetlands.

Many studies have shown that biodiversity regulates multiple ecological functions that are needed to maintain the productivity of a variety of ecosystem types. What is unknown is how human activities may alter the 'multifunctionality' of ecosystems through both direct impacts on ecosystems and indirect effects mediated by the loss of multifaceted biodiversity. Using an extensive database of 72 lakes spanning four large Neotropical wetlands in Brazil, we demonstrate that species richness and functional diversity across multiple larger (fish and macrophytes) and smaller (microcrustaceans, rotifers, protists and phytoplankton) groups of aquatic organisms are positively associated with ecosystem multifunctionality. Whereas the positive association between smaller organisms and multifunctionality broke down with increasing human pressure, this positive relationship was maintained for larger organisms despite the increase in human pressure. Human pressure impacted multifunctionality both directly and indirectly through reducing species richness and functional diversity of multiple organismal groups. These findings provide further empirical evidence about the importance of aquatic biodiversity for maintaining wetland multifunctionality. Despite the key role of biodiversity, human pressure reduces the diversity of multiple groups of aquatic organisms, eroding their positive impacts on a suite of ecological functions that sustain wetlands.

Human Activity Behind the Unprecedented 2020 Wildfire in Brazilian Wetlands (Pantanal)

The Pantanal natural region in South America is the largest wetland worldwide. Due to floods and a diverse lithology, this wetland area exhibits high plant and animal biodiversity levels and encompasses more than 185,000 km2. In 2020, the Pantanal experienced the worst recorded fire episode in history. Hundreds of thousands of hectares burned, threatening habitats and corresponding ecosystem functions. The fire reached regions never burned before, including national conservation units. Although federal authorities have blamed climate change and cultural indigenous practices, environmentalists, scientists, and non-governmental organizations have questioned this foregone conclusion. To better understand this historical event, we examined the burn severity in conservation units most affected by fire and the association with human and climate factors (represented by the fire occurrence distance to roads/waterways/railways and drought severity, respectively) and analyzed fire spatial patterns and durations. Via a comparison of these two factors, we demonstrated that 60% of the fire outbreaks was concentrated at distances less than 5 km from roads, waterways, and railways and that 80% was concentrated at distances less than 10 km from areas with human activities. Since values of the SPEI (drought index) < −2.6 were critical to the spread of fire, a combination of more favorable climate conditions enabled the rapid and irreversible spread of fire. The observed association between fire occurrence and distance to roads makes the ongoing plans for road expansion of great concern, considering the 2020 fire episode, which greatly affected natural vegetation and conservation units across the Pantanal. The determination of trends in fire hot spot regions in the Pantanal can help environmental surveillance and fire control.

Wetlands in Brazil: classification, floristic composition and biological Nitrogen fixation

Wetland ecosystems represent about 20% of South America, and are classified according to the flood regime, which also influences on vegetation. Despite the value of ecosystem services provided by this environment, those areas are close to eradication in several parts of Brazil. These environments are extremely fragile. Flooded areas are subject to nitrogen losses (N) by leaching, becoming dependent on the N increases from biological nitrogen fixation (BNF). However, little is known about this process on wetlands. Understanding the adaptative strategies of these microorganisms and plants is essential for the maintenance and preservation of these ecosystems. The objective of this work is to present a literature review discussing aspects of floristic composition, biological nitrogen fixation, and morphophysiological adaptations that occur in the rhizobium-leguminous system in wetlands. For the bibliographic survey, articles and other academic works relevant to the topic were selected, in order to enrich the proposed discussion.

Uso de plantas para nidificação e estação reprodutiva de duas espécies de curicacas (Theristicus) no Pantanal, Brasil

ABSTRACT Six species of the Family Threskiornithidae are found in the Pantanal. Despite being abundant and conspicuous birds, information on aspects of their breeding biology in this wetland remains scarce. The aim of this study was to examine breeding aspects (nest support plants and breeding season) of the two species of the genus Theristicus Wagler, 1832 that are found in the Brazilian wetland. Field work occurred in 2008, and a search for records (photographs) with evidences of breeding activities was done in the WikiAves and eBird databases in March 2020. A total of 49 records were obtained between 2007 and 2019 in four municipalities, mostly in Poconé. Records (n = 37) involving breeding Plumbeous Ibis, Theristicus caerulescens (Vieillot,1817), were obtained between March and November, and incubation has been documented mainly between May and September. Records (n = 12) of the Buff-necked Ibis, Theristicus caudatus (Boddaert, 1783), occurred between April and December, and nests with an incubating adult or nestlings were mainly found between September and December. Breeding activities of T. caudatus were delayed in relation to those of T. caerulescens. Nests of both species were platforms built with large amounts of branches. Most nests of T. caudatus were built in palms, while nests of T. caerulescens were found mainly in non-palm trees. Both species nested in tree species that are commonly found in areas where they forage.

Feeding Ecology of the Chilean Flamingo Phoenicopterus chilensis (Aves: Phoenicopteridae) in a coastal wetland in southern Brazil

ABSTRACT This study describes the diet of the Chilean Flamingo Phoenicopterus chilensis in Lagoa do Peixe lagoon, a contranuptial area. The diet was evaluated through the analyses of undigested structures present in 91 droppings collected in feeding sites around the lagoon. It was possible to identify 268 prey items that belonged to six taxa (Calanoida, Laeonereis acuta, Alitta succinea, Nereis sp., Diptera, and Ostracoda). The most frequent prey in the faeces samples of the Chilean Flamingo were Calanoida, Laeonereis acuta, Alitta succinea, and Nereis sp. Additionally, through the analyses of sediment and water samples, we characterised the potential prey available for the Chilean Flamingo in the lagoon. All available taxa that were confirmed as prey items in the faecal samples of the Chilean Flamingo were observed in high abundances during the spring, summer, and the beginning of autumn in the environmental samples. Despite the effectiveness of the microscopical faecal analysis, the method may underestimate soft-body prey and reduce the number of identified taxa in the faecal samples. Therefore, the use of complementary methodologies, such as stable isotope analysis and molecular techniques, are recommended.

Diversity and distribution of the genus Hyalella (Crustacea: Amphipoda: Hyalellidae) in temporary wetlands from the southern Brazilian Coastal Plain, with a taxonomic key to the species in the region

ABSTRACT The knowledge of the diversity and distribution of Hyalella species is scarce in the subtropical regions of Brazil. The present study investigated the diversity and distribution of Hyalella species in subtropical temporary wetlands in the southern Brazilian Coastal Plain. Six species were registered in the study region, including three new species of the genus. The spatial distribution of the species in the study region suggests that the hydrographic region (or freshwater ecoregion) is an important driver of the geographic distribution of the species. Thus, the present study increases to 19 the diversity of species with distribution in the southern region, with one new record for the state of Santa Catarina, one for the state of Rio Grande do Sul, and one for both states. We also expanded the distribution ranges of H. bonariensis, H. castroi, and H. kaingang. In addition, we elaborated a taxonomic key to the species of Hyalella known to southern Brazil. Thus, we demonstrate the need for more taxonomic studies to better understand the diversity of macroinvertebrates in Brazilian wetlands, in order to protect our fauna and contribute to the development of future conservation policies.

Macrohabitat classification of wetlands as a powerful tool for management and protection: The example of the Paraná River floodplain, Brazil

The construction of four large reservoirs for hydroelectric power generation in the main channel of the Paraná River and many others in the tributaries has eliminated important wetlands, except over an area of ∼2000 km2 between the reservoirs of the UHE Engenheiro Sérgio Motta (Porto Primavera) and the Itaipu. The up-river reservoirs created large changes in the river's hydrology and in its loads of suspended, bed and dissolved material. A detailed assessment of the impact of these changes requires a description and classification of the floodplain's different subunits, called macrohabitats, according to hydrological, hydromorphological, and vegetational parameters. The classification system presented herein is hierarchical and is embedded in the classifications of Brazilian wetlands. The system differentiates the Paraná River floodplain into 6 functional units, 13 subclasses, and 23 macrohabitats, which are presented in this paper. Changes in hydrology have reduced the flood amplitude, frequency and permanence, and thus confined the macrohabitats to an area currently subjected to a regular flood pulse of ∼3 m rather than the previous ∼6 m. The gradual terrestrialization of macrohabitats at the upper end of the flood gradient is expected, accompanied by a loss of macrohabitats at its lower end because of long-term flooding. The retention of sediments and dissolved nutrients in the reservoirs will lead to the oligotrophication of aquatic macrohabitats, the degradation of near-channel hydromorphic structures and their vegetation, and a general reduction of overall primary production in the floodplain, which may extend also to higher trophic levels.

Late Pleistocene-Holocene diatomites from the coastal plain of southern Brazil: Paleoenvironmental implications

The southern Brazilian coastal area harbors one of the largest wetland systems of South America, important for its plant and animal biodiversity and ecosystems services provided for human populations. As an attempt to understand the development of those wetlands, samples from a diatom ooze and indurated diatomites found in the coastal plain of the state of Rio Grande do Sul were analyzed. The fragmented state of the frustules found in the samples allowed identification of most diatoms only to the genus level, nevertheless the assemblages are represented by 16 genera reported today in freshwater bodies in the studied region. The identified taxa include the planktonic Aulacoseira and Cyclotella and several benthic genera, mostly Eunotia, Pinnularia and Rhopalodia. The diatomites consist mostly of corroded irregular-shaped silica grains from frustules dissolved by alkaline water, but five diatom genera were identified. These diatomites accumulated in ephemeral ponds developed on loess sediments in dry environments and seasonal climate between the latest Pleistocene and Middle Holocene. The diatom ooze and associated organic-rich silty sediments accumulated in shallow lakes with abundant catchment vegetation and considerable dissolved organic carbon, and contain fragmented but slightly dissolved frustules as a result of hydrodynamics and low pH, and also megascleres of Porifera, cysts of Chrysophyceae, and abundant phytoliths of Poaceae. The sediment underlying the diatom ooze was dated as of 4.6–4.4 cal 14C ka BP and contains a diatom assemblage dominated by benthic epiphytic and acidophilous forms such as Eunotia, Pinnularia, and Gomphonema. The ooze and sediment overlying it contain an assemblage dominated by the planktonic Aulacoseira and Cyclotella, indicating ≥1 m-deep water column subject to moderate wind-driven hydrodynamics and variable water table. This succession shows that the development of the modern wetlands during the Middle Holocene, accompanied by the expansion of vegetation, resulted from a combination of high sea-level and increased rainfall and productivity driven by the wetter and warmer climate of the Holocene Hypsithermal, although the dense accumulation of frustules in the diatom ooze suggests a drier episode around ~4 ka BP. The results show that diatom assemblges preserved as diatomites can be used to understand the origins and response of the southern Brazilian wetlands to environmental changes, which may help address the extent of anthropogenic influence and response to future climate change, thereby contributing for the conservation and management of the coastal ecosystems.

Terrain units, land use and land cover, and gross primary productivity of the largest fluvial basin in the Brazilian Amazonia/Cerrado ecotone: The Araguaia River basin

Integrity of most of tropical wetlands is threatened because they are often considered freely available resources of land and water. The Bananal, in the Araguaia River Basin, and the Pantanal in the Paraguai River Basin, are the two largest Brazilian wetlands influenced by the tropical seasonal floods. The purpose of this study is to integrate landscape structural and functional factors with the GPP dynamics over different terrain units present in the Araguaia River Basin. The study was conducted through the integration of different databases, including the topographic domains, protected areas and indigenous lands; land use and land cover map (year 2016); time series of GPP derived from the Moderate Resolution Imaging Spectroradiometer (MODIS) sensor (period of 2000–2015); Shuttle Radar Topography Mission (SRTM) digital elevation models (DEM); and precipitation data produced by the WorldClim version 2 dataset. GPP time series were processed using statistical methods of time series decomposition. The results show that, in the alluvial domains, we find grasslands (mostly), alluvial forests with the highest levels of GPP, and the largest legally protected areas. Spatial distribution of pasturelands across the entire basin is broad while croplands are concentrated mainly in geologically old and high plateaus. The time series analysis revealed the presence of intra- and inter-annual vegetation patterns and a continuous increase of GPP over time. The results contribute to the understanding of the linkages between land use changes and global carbon cycles by considering land use as a response of how the landscape is organized. The results may provide subsidies for public policies for the carbon budget equilibrium, conservation of biodiversity, and rational use of natural resources.

Age-associated hemogram and ultrastructural leukocyte morphology in Pygocentrus nattereri (Characiformes: Serrasalmidae) from the Brazilian Pantanal

ABSTRACT Pygocentrus nattereri is a carnivorous fish widely distributed in the Brazilian wetland and occupies different river strata. Based on the sexual maturity, the standard length (SL) has been used to categorize age and physiological attributes. However, hematological parameters and their relationship with age and growth features are unknow. Here, the aim was to evaluate the hematological profile and leukocyte data of juvenile and adult specimens, associating it with SL. Specimens of both sexes were weighted, and SL were measured for classify as juveniles (SL ≤16.0 cm) and adults (SL ≥16.1 cm). Blood was collected to perform the hemogram, for leukocyte quantification and ultrastructural descriptions. In general, hemogram values and leukocyte, lymphocyte, and monocyte counts were high in juveniles. Hemogram variables were negatively correlated with SL. Thrombocyte and neutrophil counts were higher in adults than in juveniles. SL was correlated with leukogram variables. Ultrastructural analysis showed that granular leukocyte count in P. nattereri was similar another fishes. PAS-positive granular leukocytes are slightly smaller than neutrophils and are characterized by dense elongated and semi-lunar shapes in the cytoplasm. Our results suggest that age-based SL may be useful for the analysis of the relationship between P. nattereri health status and wetland aquatic environments.

Dermatobiosis in Panthera onca: first description and multinomial logistic regression to estimate and predict parasitism in captured wild animals.

Dermatobia hominis is a parasite widely distributed in neotropical regions. The parasitic phase of the cycle is characterized by the formation of a subcutaneous nodule in the host, which can promote infestation by other dipterans and skin infections. The aim of this report is to register parasitism by D. hominis in free-ranging Panthera onca captured in the Brazilian wetland and to determine significant biological and meteorological factors that are likely to influence the presence of larval parasitism in captured wild jaguars. Between 2011 to 2020, 34 jaguars were captured and examined manually by searching for lesions characteristic of myiasis. By manual compression in the subcutaneous nodules, larvae morphologically identified as D. hominis (first and third instars) were collected from 13 jaguars. A multinomial logistic regression showed that adult jaguars had 16.49-fold higher odds of being parasitized than subadults. Thus, jaguars captured in the season of July-September have 34.01- and 11.42-fold higher odds of being parasitized compared to the seasons of October-December and April-June, respectively, which is associated with high total monthly precipitation in the previous season. The present study is the first to describe parasitism by D. hominis larvae in jaguars.

Free-living Microturbellarians (Platyhelminthes) from Wetlands in Southern Brazil, with the Description of Three New Species.

The Taim strict nature reserve (ESEC Taim) is a protected wetland (Ramsar site) in southern Brazil. Although the ESEC Taim harbours a broad conspicuous flora and fauna, this area lacks studies on free-living Platyhelminthes. Here, we describe three new species to science, one belonging to Macrostomorpha (Macrostomum itai n. sp.) and two belonging to Dalytyphloplanoida (Gieysztoria duopunctata n. sp. and G. hermes n. sp.). Macrostomum itai n. sp. differs from their congeners for its distinct stylet in "J"-shaped. Gieysztoria duopunctata n. sp. is distinguishable based on the crown-like penis stylet, this species possesses 12-18 spines of similar sizes and two smaller spines (one a bit longer than the other). Gieysztoria hermes n. sp. clearly differs from its congeners owing to its fibrous girdle, which bears two groups of complexes of spines and three different-in-length ventral spines. Moreover, we identified twenty-three species, eleven belonging to Catenulida, three to Macrostomorpha, seven to Dalytyphloplanoida, one to Kalyptorhynchia, and one to Prorhynchida. We recorded three species for the first time in Brazil (Myostenostomum vanderlandi, Macrostomum quiritium and Strongylostoma elongatum). This new information on these free-living Platyhelminthes makes this protected area a more valuable and reinforces the actions that are being done to protect it.

Diversity of insect galls from Mato Grosso State, Brazil: North Pantanal

Abstract: The Pantanal Biome occupies 20% of the Brazilian territory extending its distribution over two Brazilian States, Mato Grosso and Mato Grosso do Sul. This Biome is one of Brazil's poorly known regions concerning insect gall and their interactions with host plants. In this study, we characterized for the first time the gall morphology, identified host plants and the gall makers from an area of Brazilian wetlands from Mato Grosso State, known as Pantanal Matogrossense. We sampled Pantanal Biome areas in Poconé municipality, along the Transpantaneira Road, Mato Grosso State, Brazil, in two expeditions, July 2012 and January 2013, with a total effort of 2 hours. We characterized 91 morphotypes of insect galls in 54 host plant species; 28 gall makers in 24 host plant species; the richest host plant families are Fabaceae, Myrtaceae, and Sapindaceae. Psidium guineense Sw. is the super host species. This area in Pantanal Matogrossense is the second in the richness of gall morphotypes (N=91) and average morphotypes/plant species (1.7), comparing phytophysiognomies. Additionally, 15 plant species are new record as host in galler-host plant interaction in the world. This number represents 30% of the total of host plant species sampled in Poconé. This inventory is new knowledge to the Pantanal Matogrossense and representing a unique testimony of insect-plant interactions consumed by the unprecedented fire that occurred in Pantanal Biome in the dry season of 2020.