Wether Lambs Research Articles

Apparent Digestibility and Nitrogen Balance in Lambs Fed Matua or Matua-Alfalfa Hay

<h2>ABSTRACT</h2> The nutritive value of Matua prairie grass (Matua), <i>Bromus willdenowii</i> Kunth, for ruminants has not been documented. Therefore, the objective of this study was to investigate the effects of Matua and Matua-alfalfa (<i>Medicago sativa</i>) hay diets on apparent digestibility and N balance in lambs. Fourteen crossbred wether lambs (BW = 38 ± 4.3 kg) were used in a metabolism study. Apparent digestibilities of DM, NDF, ADF, and CP, and N balance were determined. The CP content of Matua-alfalfa mixed hay and Matua hay were11.26 and 10.88%, respectively. Regarding fiber components, NDF was 70.52 and 73.37%, and ADF was 40.99 and 41.94% in the Matua-alfalfa mixed and Matua hays, respectively. Apparent digestibilities of DM, NDF, and ADF were not affected by diet. Apparent digestibility of CP in lambs was 35.24% higher (<i>P</i> < 0.05) for the Matua-alfalfa mixed hay than the Matua hay. Fecal and urinary N excretion increased (<i>P</i> < 0.05) in the lambs fed Matua versus Matua-alfalfa hay by 28.35 and 7.29%, respectively. Nitrogen retention was 92.27% higher (<i>P</i> < 0.05), also as a result of feeding Matua-alfalfa versus Matua hay to lambs. Animal performance may be improved because of improved N utilization and nutritive value through incorporation of alfalfa into Matua stands.

Effects of Feeding Different Protein Supplements on Digestibility, Nitrogen Balance and Calcium and Phosphorus Utilization in Sheep

Two metabolism trials were conducted with 24 wether lambs to investigate the effects of feeding crab meal and other protein supplements on N utilization, digestibility and Ca and P balance in sheep. The lambs (avg. BW, 25 kg) were randomly allotted to eight diets in each of two trials. The supplements were: i) none, negative control (NC); ii) soybean meal (SBM), control; iii) supplement based on industrial byproducts of both plant and animal origin (IPA); iv) experimental supplement based on byproducts of animal origin (ESA); v) hydrolyzed supplement No 4. (HESA); vi) commercial supplement based on animal protein (CS), Pro-Lak®; vii) crab meal (CM); and viii) urea (U). The supplements supplied 33% of the total dietary N (CP, 9.8%; DM basis). Lambs fed the NC diet had lower (p<0.05) DM and OM digestibility. Lower (p<0.05) apparent absorption of N was. recorded for the lambs fed the HESA and NC diets. Sheep fed CM had lower Ca absorption compared to SBM. Highest (p<0.05) P absorption was observed for lambs fed CS and CM and lowest for U and NC diets. Sheep fed CM had higher (p<0.05) total VFA concentration (65.7 μmol/ml), compared to those fed ESA, CS, and NC diets (47.3, 49.8, and 49.5 pmol/ml, respectively). Highest (p<0.05) ruminal NH 3 N (29.6 mg/dl) was observed in lambs fed the U diet, while those fed the NC diet had the lowest (p<0.05) average value (7.66 mg/dl). Lambs fed the U diet had the highest (p<0.05) blood urea N (10.67 mg/dl). The present study showed that N utilization of diets supplemented with experimental supplements based on feather meal and blood meal; commercial supplement based on animal protein, Prolak®; supplement based on plant protein and blood meal; and crab meal are comparable with that of soybean meal.

Effect of dietary cation-anion difference on urinary pH, feedlot performance, nitrogen mass balance, and manure pH in open feedlot pens1

Six experiments were conducted to evaluate dietary cation-anion difference (DCAD) in concentrate diets on urinary pH, feedlot performance, and N mass balance. In Exp. 1, 15 wether lambs (33.5 ± 3.0 kg) in five 3 × 3 Latin squares were fed a basal diet of 82.5% dry-rolled corn (DRC), 7.5% alfalfa hay, 5% molasses, and 5% supplement with different proportions of anionic and cationic salts. The DCAD was -45, -24, -16, -8, 0, +8, +16, +24, +32, and +40 mEq per 100 g of DM with the control basal diet (DCAD = +8) included in each square. Urinary pH increased (cubic, P < 0.01) as DCAD increased and DMI increased linearly (P < 0.01) with increasing DCAD. In Exp. 2 and 3, 8 Holstein steers (312 ± 24 kg) were used in 2 consecutive 4 × 4 Latin squares. Steers were fed either the same basal diet as Exp. 1 or a basal diet with 20% wet distillers grains (WDGS) replacing DRC. In Exp. 2, DCAD was adjusted to -2, -12, and -22 mEq per 100 g of DM from the basal diet (DCAD = +8) and DCAD was adjusted in Exp. 3 to -12, -22, and -32 mEq per 100 g of DM from the basal WDGS diet (DCAD = -2). Urinary pH decreased linearly as DCAD decreased (P < 0.01) in both experiments, whereas DMI decreased linearly in Exp. 2 (P = 0.02) but not Exp. 3 (P = 0.96). In Exp. 4, 6 crossbred steers (373 ± 37 kg) were used in a 2-period crossover design. Steers were fed the same basal diet as Exp. 3 with DCAD of -16 (NEG) and +20 (POS) mEq per 100 g of DM. Urinary pH and DMI (P < 0.05) were less for cattle fed the NEG diet compared with POS. In 2 experiments, steers (n = 96 each) were fed NEG or POS as calves (260 ± 22 kg of BW) for 196 d from November to May (Exp. 5) or as yearlings (339 ± 32 kg of BW) for 145 d from June to October (Exp. 6). Final BW, DMI, ADG, and HCW were not different (P > 0.11) among treatments in either experiment. Efficiency of BW gain was increased (P = 0.05) for steers fed NEG compared with POS in Exp. 5 but was not different (P = 0.11) in Exp. 6. Amount of N intake, retention, excretion, and manure N (kg/steer) were not different (P > 0.11) among treatments in either experiment. Manure pH (soil, feces, and urine) was decreased (P < 0.01) in pens fed NEG compared with POS in both experiments. Amount of N lost (kg/steer) was not different (P = 0.44) in Exp. 5, but tended (P = 0.09) to be 10.6% greater for POS compared with NEG in Exp. 6. Urinary pH was decreased by reducing DCAD, but this had minimal effect on N losses in open feedlot pens in these experiments.

Lack of response to garlic fed at different dose rates for the control of Haemonchus contortus in Merino wether lambs

With the increased incidence of parasite resistance to chemical anthelmintics worldwide novel approaches to manage parasite infection, such as medicinal plants and their extracts, are being investigated by the scientific community. The current study tested the effect of three rates of garlic (0.9, 1.8 and 3.6%) in a pelleted ration on Haemonchus contortus in sheep. Thirty-nine Merino wether lambs aged 6 months were divided into five treatment groups, including three garlic dose rates and two control groups that received no garlic. All animals were infected with 4000 L3 H. contortus larvae 3 weeks after allocation to treatments. A positive control group was drenched with abamectin 28 days after infection. The synthetic drench was effective in controlling the parasites, but there was no reduction in either worm egg counts (WEC) or total worm count due to the garlic. The 3.6% garlic treatment had significantly lower (P &lt; 0.05) liveweight, feed intake, body condition score and feed conversion ratio than any of the other treatment groups, suggesting that this level of garlic had a low level of anti-nutritional properties. There was an interaction between faecal WEC and voluntary feed intake over time, with the animals with higher voluntary feed intake having lower WEC over time.

Influence of vitamin E supplementation and basal diet on the vitamin E status, performance and tissue fatty acid concentration in lambs

In order to determine the effect of dietary vitamin E level and basal diet on vitamin E status, performance and tissue fatty acid content, five groups of eight Suffolk × Charollais wether lambs with an initial live weight of 28.4 (s.d. 1.6) kg were allocated to one of five concentrate-based diets supplemented with all-rac-α-tocopheryl acetate to contain 30 mg (C-30), 60 mg (C-60), 120 mg (C-120), 250 mg (C-250) or 500 mg (C-500) α-tocopheryl acetate/kg dry matter (DM), for 63 days. Two additional groups of eight lambs entered the study at 31.2 (s.d. 3.3) kg and were fed grass silage and 400 g/day concentrate for 56 days, with the whole diet providing the equivalent of 60 mg (S-60) or 500 mg (S-500) α-tocopheryl acetate/kg DM. Lambs were weighed and blood samples obtained by venipuncture weekly. Dietary vitamin E level did not affect performance (P > 0.05), but lambs fed grass silage grew more slowly (P < 0.001) and had a higher (P < 0.001) feed conversion ratio (kg feed/kg gain) than those fed concentrates. At day 0 plasma α-tocopherol concentrations were 0.8 μg/ml and did not differ between treatments (P > 0.05). Plasma α-tocopherol concentrations then decreased in all lambs except for those fed S-500, which increased, and at slaughter were (μg/ml) 0.07, 0.23, 0.39, 0.76 and 1.57 in C-30, C-60, C-120, C-250 and C-500 and 1.18 and 1.93 in S-60 and S-500, respectively. At slaughter, muscle and liver α-tocopherol concentrations were in the deficiency range for lambs fed C-30, C-60 or C-120, whereas plasma creatine kinase and tissue polyunsaturated fatty acids were unaffected by dietary vitamin E level, but creatine kinase levels were higher (P < 0.05) and glutathione peroxidise levels lower (P < 0.001) in lambs fed grass silage than concentrates alone. Muscle and liver α-tocopherol concentrations were 1.8- and 4.1-fold higher in lambs fed S-60 than C-60, but there was less of a difference between lambs fed S-500 or C-500 with muscle and liver differences of 0.4- and 0.7-fold, respectively. Tissue n-3 polyunsaturated fatty acid concentrations were higher (P < 0.05) and n-6 fatty acids lower in lambs receiving the grass silage compared to concentrate-based diets, but were not affected by dietary vitamin E level. It is concluded that lower plasma and tissue levels of α-tocopherol are present in lambs supplemented with all-rac-α-tocopheryl acetate on a concentrate compared to a mixed diet of silage and concentrates, and that normal growth can be achieved at tissue levels previously considered to represent deficiency.

Nutrient digestion and performance by lambs and steers fed thermochemically treated crop residues

Five studies were conducted to determine nutrient digestibility and performance of lambs and steers fed thermochemically treated crop residues and distillers dried grains with solubles (DDGS) as a corn replacement pellet (CRP; 75% residue:25% DDGS, DM basis). Fifteen Hampshire, Suffolk, or Dorset wethers (BW 33.3 +/- 5.0 kg) were utilized to evaluate nutrient digestibility of the unprocessed native (NAT) and CRP [Exp. 1: wheat straw (WS); Exp. 2: corn stover (CS); Exp. 3: switchgrass (SWG) and corn fiber:wheat chaff (CFWC)] when limit fed (Exp. 1 and 2: 1.8% of BW daily; Exp. 3: 2.5% of BW daily) compared with a 60% corn diet. In Exp. 4, 56 individually fed Dorset-cross wether lambs (BW 32.0 +/- 1.4 kg) were utilized to compare performance and digestibility of WS, wheat chaff (WC), corn fiber (CF), a 3:1 blend of corn fiber:wheat straw (CFWS), a 3:1 blend of CFWC, and SWG-CRP fed for ad libitum intake compared with a 45% corn diet. In Exp. 5, 32 individually fed Holstein steers (BW 185.2 +/- 0.9 kg) were used to evaluate performance and digestibility of diets containing corn, WS-CRP, CFWC-CRP, or NAT-WS fed for ad libitum intake. Crop residues were processed with 5% calcium oxide (DM basis) and 35% water in a double-shaft enclosed mixer (Readco Kurimoto Continuous Processor, York, PA) and subsequently pelleted with DDGS to form CRP. Feeding lambs WS-CRP (Exp. 1) or CS-CRP (Exp. 2) increased digestion of DM, NDF, and ADF compared with NAT (P < 0.05). In Exp. 3, feeding CFWC-CRP increased total tract NDF digestibility and ADF digestibility (P < 0.05). Experiment 4 final BW were greatest for control lambs and least for lambs fed CFWS-CRP or SWG-CRP. Body weight gains for lambs fed CRP averaged 15.9% less than control (P < 0.05). Lambs fed CRP diets had greater (P < 0.05) NDF and ADF intake and output. In Exp. 5, steers fed the corn or 2 CRP diets gained similarly and faster (P < 0.05) than those fed the NAT-WS diet. Steers fed the control corn diet were more efficient (P < 0.05) than steers fed other treatments. Steers fed the corn diet, CFWC-CRP, and WS-CRP had greater (P < 0.01) DM digestibility than NAT. The results confirm benefits for nutrient digestion and subsequent animal performance when crop residues are thermochemically processed. Processed crop residues may be fed in combination with DDGS to partially replace corn in ruminant diets.

Effect of protein supplementation on expression and distribution of urea transporter-B in lambs fed low-quality forage1

Two experiments were conducted to determine the effects of ruminal protein degradability, supplementation frequency, and increasing dietary protein on the expression and distribution of urea transporter-B (UT-B) in lambs fed low-quality forage (mature crested wheatgrass hay; 4.2 to 4.7% CP). In Exp. 1, 15 Dorset wether lambs (initial BW=45.8+/-1.3 kg) were blocked by initial BW and assigned to 1 of 3 treatments within a randomized complete block design for 28 d, with supplements fed to achieve 7, 10, or 13% total dietary CP. In Exp. 2, 13 Dorset wether lambs (initial BW=34+/-4 kg) were used in a completely randomized design and given 1 of 4 isonitrogenous supplements: 1) ruminally degradable protein (RDP) fed daily (n=3), 2) RDP fed on alternate days (n=3), 3) ruminally undegradable protein (RUP) fed on alternate days (n=3), or 4) a 50:50 mixture of RDP and RUP fed on alternate days (n=4) for 18 d. Alternate-day treatments were fed at twice that of daily supplementation. On the last day of both experiments, lambs were killed and samples taken for Western blot analyses for UT-B. Immunoblotting using a rabbit polyclonal antibody to UT-B confirmed the presence of distinct 32-kDa (consistent with a nonglycosylated UT-B protein) and 47-kDa (probable N-glycosylated form of UT-B) protein bands in all 9 tissues analyzed. In both experiments, the liver, dorsal rumen, reticulum, and ventral rumen displayed strong bands at 32 kDa and lighter bands at 47 kDa, whereas the cecum, large colon, spiral colon, and parotid salivary gland displayed slight 32-kDa bands and stronger, more visible bands at 47 kDa. Both protein bands were apparent in the kidney at similar visual intensities in Exp. 1, whereas the relative intensities of the 2 UT-B bands in the kidney were variable, and appeared somewhat reciprocal among animals in Exp. 2. Although the abundance of the 47-kDa UT-B band in the ventral rumen was greater (P=0.03) in lambs fed RDP daily in Exp. 2, no other treatment differences (P >or= 0.15 to 0.99) in the abundance of the 32- or 47-kDa UT-B proteins within tissues were observed in either experiment. Although protein supplementation strategy had little effect on UT-B expression in tissues other than the ventral rumen, differences in the degree of glycosylation of UT-B across tissues may provide insight into its regulation.

Effect of Including Alfalfa Hays That Were Harvested in the Morning or Evening in Diets of Newly Received Sheep

<h2>ABSTRACT</h2> In previous experiments, ruminants preferred evening-harvested (PM-alfalfa) over morning-harvested (AM-alfalfa) harvested alfalfa hay when given a choice. Therefore, could strategic use of PM-alfalfa fed as a pellet or included as a component of receiving diets stimulate naive sheep to increase DMI early in the receiving period? Experiments were conducted to 1) determine preferences of naive sheep for pellets prepared from PM- and AM-alfalfa, 2) measure performance of wether lambs fed pellets prepared from PM- and AM-alfalfa, and 3) measure performance and productivity of ewe lambs fed receiving diets formulated with PM- and AM-alfalfa. Pelleting PM-alfalfa resulted in pellets having similar (P > 0.05) palatability to naive sheep as pellets prepared from AM-alfalfa. Inclusion of PM-alfalfa in receiving diets fed to ewe lambs resulted in greater consumption of alfalfa stem fractions (P < 0.01). However, this effect did not result in greater DMI or gain over a 35-d feeding period (P > 0.76). Based on data from this study, strategic dietary use of pellets or hay prepared from evening-harvested alfalfa did not stimulate naive sheep to increase DMI. Lack of commonality between experiments reported herein and other research may be because 1) sheep cannot discriminate as well as other livestock between AM- and PM-alfalfa, or 2) pelleting or including in mixed diets masks sheep-preferred characteristics of PM-alfalfa. Based on the alfalfa hay used in these experiments, it was not advantageous to use pellets or hay prepared from evening-harvested alfalfa for the specific purpose of encouraging naive sheep to increase DMI.

Limiting amino acids for growing lambs fed a diet low in ruminally undegradable protein1

Ruminally cannulated Rambouillet wether lambs were used in three 6 x 6 Latin square experiments (n = 6/experiment) to determine which essential AA limit N retention. Lambs (BW = 36.9 +/- 1.9 kg for Exp. 1, 35.1 +/- 1.4 kg for Exp. 2, and 46.0 +/- 1.3 kg for Exp. 3) were housed in metabolism crates and limit-fed (DMI = approx. 1.8% of BW daily) twice daily a soybean hull-based diet low in ruminally undegradable protein. Treatments for Exp. 1 were continuous abomasal infusions of a solution (500 mL/d) containing 1) no AA (CON), 2) a mixture of 10 essential AA and 2 nonessential AA (10EAA), 3) 10EAA with Met removed, 4) 10EAA with Lys removed, 5) 10EAA with His removed, and 6) 10EAA with Thr removed. Treatments for Exp. 2 were abomasal infusions of 1) CON, 2) 10EAA, 3) 10EAA with Leu, Ile, and Val removed (-BCAA), 4) 10EAA with Arg removed, 5) 10EAA with Phe removed, and 6) 10EAA with Trp removed. Treatments for Exp. 3 were abomasal infusions of 1) CON, 2) 10EAA, 3) -BCAA, 4) 10EAA with Leu removed, 5) 10EAA with Ile removed, and 6) 10EAA with Val removed. All lambs received continuous infusions of acetate and propionate into the rumen and dextrose into the abomasum to supply additional energy. Periods were 7 d: 3 d for adaptation to abomasally infused treatments and 4 d for fecal and urinary collections. Blood samples were collected 3 h after feeding on d 7. In all 3 experiments, N retention was greater (P < 0.10) for lambs receiving 10EAA vs. CON, demonstrating that the basal AA supply from CON was limiting. Removal of each of the essential AA from 10EAA decreased (P < 0.10) their concentrations in plasma (except for Trp), indicating that 10EAA supplied these AA in excess of the animal's requirement. In Exp. 1, N retention (g/d) decreased (P < 0.10) in response to the removal of Met and Thr, but was not affected by removal of Lys and His from 10EAA. In Exp. 2, N retention decreased (P < 0.10) in response to removal of all 3 branched-chain AA, Arg, and Trp, whereas the removal of Phe from 10EAA did not affect N retention. In Exp. 3, N retention decreased (P < 0.10) in response to removal of branched-chain AA and Val, but was not affected by the omission of Leu and Ile from 10EAA. The results of this research demonstrated that Met, Thr, Arg, Trp, and Val limited N retention of lambs fed a diet low in ruminally undegradable protein.

Effects of Dietary Copper Source and Level on Performance, Carcass Characteristics and Lipid Metabolism in Lambs

An experiment was conducted to determine the effect of dietary copper (Cu) on performance, carcass characteristics and lipid metabolism in lambs. Fifty DorperxMongolia wether lambs (approximately 3 month of age; 23.8±0.6 kg of body weight) were housed in individual pens and were assigned randomly to one of five treatments. Treatments consisted of 1) control (no supplemental Cu), 2) 10 mg Cu/kg DM from Cu-lysine, 3) 20 mg Cu/kg DM from Cu-lysine, 4) 10 mg Cu/kg DM from tribasic copper chloride (Cu 2 (OH) 3 Cl; TBCC), 5) 20 mg Cu/kg DM from tribasic copper chloride. The Cu concentration was 6.74 mg/kg DM in the basal diet. Body weight was measured on two consecutive days at the start and the end of the 60-day experimental period. Blood samples were collected and then the lambs were slaughtered on d 60. Performance was not affected (p>0.05) by dietary Cu treatment. Cu-supplemented and control lambs had similar hot carcass weight, dressing percentage and longissimus muscle area, but Cu supplementation, regardless of source and level, reduced (p 0.05) by dietary treatment. Fatty acid profile of longissimus muscle was similar across treatments. These results indicate that Cu-lysine and TBCC are of similar availability in lambs. Cu supplementation given to DorperxMongolia wether lambs altered lipid metabolism. The reduction in backfat depth may be due to copper altering TNF-a metabolism in lambs. Supplementation of 10 or 20 mg Cu/kg DM showed similar effects on lipid metabolism in lambs.

Intake and digestion of wheat forage by stocker calves and lambs

Because wheat forage contains high concentrations of N, NPN, digestible DM, and water, beef cattle and sheep require an adaptation period before positive BW are seen. The objective of the present experiment was to determine the impact of length of exposure of lambs and steers to wheat forage on BW gains, N retention, and forage digestibility. Sixteen steer calves (average BW = 210 +/- 12 kg) and 20 wether lambs (average BW = 31.5 +/- 2.0 kg) were randomly assigned to 1 of 2 treatment groups. Group 1 grazed a wheat pasture for 120 d during the winter, whereas group 2 was wintered on dormant warm-season grass pastures plus warm-season grass hay and plant-based protein supplements. In the spring (April 5), all lambs and steers grazed wheat pasture for 14 d and were then housed in metabolism stalls and fed freshly harvested wheat forage to determine forage digestibility and N metabolism. Data were analyzed for lambs and steers separately as a completely randomized design, using the individual animal as the experimental unit. Lambs and steers grazing wheat pasture for the first time in the spring had less ADG during the first 14 d than lambs (80 vs. 270 g, respectively; P = 0.01) and steers (1.06 vs. 1.83 kg, respectively; P = 0.09) that had grazed wheat pastures all winter. Digestibility of DM, NDF, and ADF fractions and N metabolism of freshly harvested wheat forage by lambs and steers were not different (P > 0.10) between the 2 treatment groups. Less ADG during the first 14 d of wheat pasture grazing is most likely the result of less DMI by nonadapted animals and is not due to diet digestibility or N metabolism.

Fumaric acid supplements have no effect on methane emissions per unit of feed intake in wether lambs

Recent reports suggest that supplementing the diet of ruminants with up to 10% fumaric acid (FA) can produce large reductions in methane (CH4) emissions, but the results have been equivocal. This trial evaluates the potential of FA to reduce enteric CH4 emissions from 18-month-old wethers fed lucerne. On two occasions, methane emissions were measured on 20 wethers fed a diet of dried ground lucerne with FA added to the diet at 0, 4, 6, 8 and 10 g FA/100 g dry matter (n = 4 wethers/treatment). Individual daily CH4 emissions were estimated using the sulfur hexafluoride (SF6) tracer gas technique together with measurements of daily dry matter intake (DMI) and total faecal output. Rumen samples were collected twice during the treatment periods in order to evaluate the effect of FA on rumen pH. There was a significant negative regression between FA% in the diet and CH4 emissions/day. However, the addition of FA did not affect CH4 emissions/kg DMI because high levels of FA supplementation reduced DMI. Rumen pH increased linearly with increasing level of FA supplementation. In conclusion, the effect of FA on CH4 emissions in this trial was associated with the resulting reduction in DMI and there were no decreases in CH4 emissions/kg DMI.

Effects of sodium chloride and betaine on hydration status of lambs at slaughter

Under commercial conditions in Australia, lambs are often dehydrated at slaughter despite access to water at the abattoir. Dehydration results in reduced fluid content of meat, which causes liveweight and carcass weight loss. Two experiments evaluated hydration status and carcass characteristics in lambs fed increased levels of sodium chloride (salt) and/or betaine before slaughter. Both experiments were 2 × 2 factorial designs with two levels of salt added [0 and 50 g/kg dry matter (DM)] and two levels of betaine added (0 and 6.7 g/kg DM) to the diets. The treatment diets were introduced to sheep over a 7–8-day period and then fed at 1.5 kg/day for the following 7 days. In experiment 1, 96 Poll Dorset–Merino cross wether lambs (45.9 ± 0.17 kg) were fed in individual pens and in experiment 2, 204 Merino wether lambs (43.1 ± 0.35 kg) were fed in group pens. In the first experiment, water and feed were withheld from the end of the treatment feeding period until slaughter 48 h later. In the second experiment, feed and water were withheld for the first 24 h after treatment but water was available for the next 24 h immediately before slaughter. In both experiments, consumption of the high salt diet decreased urine specific gravity and osmolarity at the end of the feeding period and after 24 h without access to water. This indicates an improved hydration status at this time. However, the difference in hydration status related to feeding salt declined during the 48-h lairage period and there were no significant differences in hot carcass weight or muscle DM among groups at slaughter. It could be concluded from this result that the addition of salt has little commercial value; however, this may not be the case. The lambs in experiment 2 did not behave according to industry expectations. They all drank water in the last 24 h in lairage and none of the groups showed severe dehydration. Further research is justified to determine if the lambs fed the diets with no added salt drunk in lairage as a learned response from the lambs fed salt and, if so, whether this can be developed into a commercial strategy. Feeding betaine did not result in any changes in hydration status or carcass characteristics.

The effect of level of intake and forage quality on methane production by sheep

In an experiment to determine the effect of level and quality of forage intake on methane (CH4) emissions, 16 wether lambs were allocated over two periods to two dietary treatments consisting of ryegrass at two stages of physiological maturity: an advanced stage of flowering and seeding (reproductive phase) and before flowering (vegetative phase). Additionally, in each period the lambs were divided into four groups and fed differing levels of food, from three-quarters maintenance to twice maintenance, to ensure a range of dry matter intakes amongst lambs. Apparent in vivo digestibility was measured and the mean values were 62.5% and 75.3% (s.e.d. = 0.84) for reproductive and vegetative ryegrass, respectively. Methane emissions were measured with the sulfur hexafluoride tracer technique. Daily methane emission was highly correlated with the amount of dry matter intake (DMI) (R2 = 0.83) and the regression was similar for both types of feed. Mean CH4 emissions per unit of DMI were 23.7 and 22.9 g/kg DMI (s.e.d. = 0.59) for reproductive and vegetative phases of ryegrass, respectively. The CH4 emissions per unit of DMI were not related to either level of DMI or diet quality.

The impact of clean fleece weight and bodyweight selection in Merinos on meat traits in the progeny

The carcasses of 136 Merino wether lambs from a single bloodline were studied to determine the impact of phenotypic selection for clean fleece weight (CFW) and bodyweight (BWT) on meat quality characteristics. The lambs were the progeny of sires and dams that were selected for high or low CFW and high or low BWT using hogget phenotypic data, where the dams were managed at 10 or 15 dry sheep equivalent/ha during gestation and from marking to weaning. Maternal stocking rate was found to have no significant effect on any meat traits, except to reduce fatness in high BWT lambs. High CFW selection led to increased glycolytic activity in muscle reflected by lactate dehydrogenase (LDH) activity. Phenotypic BWT selection increased hot carcass weight and decreased fatness, lowered temperature at pH 6.0, and showed considerable variation in LDH activity and the ratio of LDH to isocitrate dehydrogenase. It is clear from this study that the stocking rate of the dam during gestation and lactation, and her selection on the basis of CFW or BWT phenotype had no large negative impact on the meat traits of her male progeny.

Feed efficiency for meat and wool production by Merino and F1 Dohne×Merino lambs fed pelleted diets of different nutritive value

There is anecdotal evidence from Western Australian breeders that Dohne sheep maintain a higher level of meat and wool production than Australian Merinos. Feed efficiency, carcass and wool attributes are moderately heritable so we hypothesised there would be differences in these traits between Merino and F1 Dohne × Merino lambs. Two groups of 15 Merino and 15 F1 Dohne × Merino wether lambs (29–40 kg) were fed a pelleted diet of either moderate or high energy and protein content for 48 days. Ad libitum pellet intake and liveweight gain were measured and the feed conversion ratio (FCR) for both wool growth and liveweight were calculated. Wool growth and quality were measured and the lambs were slaughtered to measure carcass and meat quality attributes. The F1 Dohne lambs were heavier at the start of the study and grew faster than the Merinos (P &lt; 0.05) irrespective of diet. Consequently, the carcasses of the F1 Dohne lambs were heavier than the Merinos (P &lt; 0.001), but the differences in FCR were not significant. Merino lambs produced more clean wool of lower fibre diameter from less feed than the F1 Dohne lambs (P &lt; 0.05). These results suggest that F1 Dohne × Merino lambs may have an economic advantage in terms of meat production, but this is traded-off against wool production.

Effects of supplemental ruminally degradable protein versus increasing amounts of supplemental ruminally undegradable protein on site and extent of digestion and ruminal characteristics in lambs fed low-quality forage1

Four ruminally and duodenally cannulated Suffolk wether lambs (34.5 +/- 2 kg initial BW) were used in a 4 x 4 Latin square designed experiment to compare effects of supplemental ruminally degradable protein (RDP) vs. increasing amounts of supplemental ruminally undegradable protein (RUP) on ruminal characteristics and site and extent of digestion in lambs. Lambs were fed a basal diet of crested wheatgrass hay (4.2% CP) for ad libitum consumption, plus 1 of 4 protein supplements: isolated soy protein (RDP source) fed to meet estimated RDP requirements assuming a microbial efficiency of 11% of TDN (CON) or corn gluten meal (RUP source) fed at 50, 100, or 150% of the supplemental N provided by CON (C50, C100, and C150, respectively). Neither NDF nor ADF intake was affected (P >/= 0.18) by protein degradability, but they increased or tended to increase (P </= 0.07) with increasing level of RUP. Total OM and N intakes were similar (P >/= 0.26) for CON and C100, but increased (P </= 0.01) as level of RUP increased. True ruminal OM and ruminal digestibilities of NDF and ADF were not affected (P >/= 0.33) by protein degradability. However, true ruminal N digestibility was greater (P = 0.03) for CON compared with C100. Ruminal ammonia concentrations were greater (P = 0.002) for CON compared with C100 lambs, and increased (P = 0.001) with increasing RUP. Microbial N flows were not affected (P >/= 0.12) by protein degradability or increasing RUP. Likewise, neither ruminal urease activity (P >/= 0.11) nor microbial efficiency (P >/= 0.50) were affected by protein degradability or level of RUP. Total tract OM, NDF, and ADF digestibility was greater (P </= 0.05) for C100 compared with CON. Likewise, total tract N digestibility was greater (P = 0.03) for C100 than for CON, and increased linearly (P = 0.001) with increasing RUP. Lambs fed C100 consumed approximately 69% less supplemental RDP (31% less total RDP) than CON, but were able to maintain forage intake and digestion. This lack of response in forage intake would suggest that lambs supplemented with RUP were recycling sufficient N to compensate for an apparent RDP deficiency. Although ruminal degradability of protein has little effect on forage intake or ruminal digestion of nutrients, there is potential to enhance total tract digestion of nutrients by decreasing the ruminal degradability of supplemental protein.

Effects of different selenium sources on tissue selenium concentrations, blood GSH-Px activities and plasma interleukin levels in finishing lambs

Thirty-two wether lambs of Tan sheep were randomly assigned into four dietary treatment groups (eight per group) for an 8-wk study and then fed a basal diet deficient in Se (0.06 mg/kg) or diets supplemented to provide 0.10 mg/kg Se from sodium selenite, selenized yeast, and seleniumenriched probiotics, respectively. Blood samples were collected at d 0, 28, and 56 of the experiment and tissue samples were collected at experiment termination. Tissue and blood Se concentrations, blood glutathione peroxidase (GSH-Px) activities, and plasma interleukin levels were analyzed. The results showed that the concentrations of Se in the kidney, liver, and muscle increased in all of the supplemented groups (p < 0.01) compared with the control group. However, the Se concentrations in the kidney, liver, and muscle in the groups supplemented with Se yeast and Se-enriched probiotics were higher than those in the group supplemented with sodium selenite (p < 0.01). The activities of GSH-Px and the concentrations of Se in blood also increased in all of the supplemented groups during the period of supplementation (p < 0.01) compared with the control group. The activities of GSH-Px and the concentrations of Se in the whole blood of the lambs fed with selenized yeast and Se-enriched probiotics were higher than those of lambs fed with sodium selenite (p<0.01 or p<0.05). The concentrations of interleukin-1 and interleukin-2 in plasma significantly increased in all of the supplemented groups during the entire period of experiment (p<0.01) compared with the control group, but had no significant differences among all of the supplemented groups. In conclusion, a diet supplemented with Se for finishing lambs was able to increase the concentrations of Se in tissue and blood, activities of GSH-Px in blood, and levels of interleukins in plasma. Organic Se sources (selenized yeast and Se-enriched probiotics) were more effective than the inorganic Se source (sodium selenite) in increasing tissue and blood Se concentrations and blood GSH-Px activities of lambs. However, there were no significant differences in plasma interleukin levels of lambs between organic and inorganic Se sources.

Effects of selenium supplementation on polyunsaturated fatty acid concentrations and antioxidant status in plasma and liver of lambs fed linseed oil or sunflower oil diets

Thirty-two, 17 weeks old, wether lambs (Chinese Poll Dorset × Small Tailed Han × Charolais), weighing 26.3 ± 1.2 kg, were used to evaluate effects of different Se levels and polyunsaturated fatty acid (PUFA) sources in the diet on fatty acid composition and antioxidant status in plasma and liver. Lambs were divided into four groups and each was fed one of the following diets: 950 g basal diet/kg DM and 50 g linseed oil/kg DM (LO); LO plus 2 mg Se/kg DM (as sodium selenite) (LOSe); 950 g basal diet/kg DM and 50 g sunflower oil/kg DM (SO); SO plus 2 mg Se/kg DM (SOSe). The experiment lasted 50 days and lambs were slaughtered at the end of it. Concentrations of C18:2 trans-10, cis-12 in liver, and C18:1 trans-11 in plasma, increased with Se supplementation, which also increased concentrations of C20:4 in lambs fed the sunflower oil diet, but not in those fed the linseed oil diet, in both plasma and liver. Fatty acid composition in plasma and liver reflected the dietary fatty acid source. Lambs fed the linseed oil diet had higher concentrations of C18:3n-3, C20:5n-3 and C22:6n-3. In contrast, lambs fed the sunflower oil diet had higher concentrations of conjugated linoleic fatty acid, C18:2n-6 and C20:4n-6. Antioxidant status increased with Se supplementation, but was not affected by PUFA source in the diets. Results indicate the possibility of increasing the PUFA deposition by combining Se with PUFA in lamb diets, and that Se increased concentrations of C20:4n-6 more efficiently when combined with the sunflower oil diet versus the linseed oil diet.

The effect of supplementation of a white clover or perennial ryegrass diet with grape seed extract on indole and skatole metabolism and the sensory characteristics of lamb

AbstractCondensed tannin in the form of a grape seed extract (GSE) was dosed to weaned wether lambs fed white clover (WC) or perennial ryegrass (PRG) over a 9‐week period to determine whether the ‘pastoral’ flavour and odour of meat could be altered. The concentrations of the pastoral flavour compounds indole and skatole were determined in the rumen fluid, blood plasma and intermuscular fat. The odour and flavour of fat and meat from the slaughtered lambs was assessed by a trained panel. The rumen fluid and blood plasma concentrations of indole and skatole were higher in those lambs fed WC compared to PRG (P &lt; 0.05) and the overall meat flavour intensity was greater when feeding WC (P &lt; 0.01). The observed concentration of indole and skatole in the fat between WC and PRG feeding treatments was not statistically different. Power analysis indicated that increasing the number of lambs per treatment group from 20 to 65 would result in a higher fat skatole concentration (P &lt; 0.05) being detected in lambs fed WC compared to PRG. Dosing with GSE gave a small reduction in skatole concentration in the rumen fluid and reduced plasma concentration of indole and skatole (P &lt; 0.001). Odour and flavour scores of the fat and meat samples were not particularly high however, dosing with GSE lowered the overall and sweet odour and the sheepy, camphor, faecal and barnyard flavour (P &lt; 0.05). Although the plasma concentration of indole and skatole suggests that GSE reduced indole and skatole formation, the intermittent supply of the GSE to the rumen environment was not sufficient to reduce their concentration in the fat. Hence, the small difference in the scores for pastoral odour and flavour attributes associated with GSE treatment may arise from other unknown factors. From a primary investigation, there was no difference in the concentration of indole and skatole in fat samples collected from carcasses before and after chilling. Further investigations into meat pastoral flavour are warranted through feeding condensed tannin‐containing forages. Copyright © 2007 Society of Chemical Industry