Wet Meadows Research Articles

Soil nitrogen-related functional genes undergo abundance changes during vegetation degradation in a Qinghai-Tibet Plateau wet meadow.

Our research investigates how the degradation of meadows affects the tiny organisms in soil that help plants use nitrogen, which is essential for their growth. In the Qinghai-Tibet Plateau, a region known for its unique ecosystems, we found that as meadows deteriorate-due to climate change and human activities-the number of these beneficial organisms significantly decreases. This decline could reduce soil fertility, impacting plant life and the overall health of the ecosystem. Understanding these changes helps us grasp how environmental pressures influence soil and plant health. Such knowledge is crucial for developing strategies to preserve these vulnerable ecosystems and ensure they continue to sustain biodiversity and provide resources for local communities.

Home range sizes and nychthemeral habitat uses by the Northern Shoveler (Spatula clypeata) on prenuptial stopovers in Vendée marshes, western France

The wetlands of Marais breton (MB) and Marais poitevin (MP) on the French Atlantic coast are commonly used by several duck species, especially as stopover sites during the prenuptial migration. Understanding the ecological requirements of Anatidae at spring stopover sites is important to define appropriate management actions that might have a carry over effect on the subsequent reproduction success. This study focused on the Northern Shoveler (Spatula clypeata), a species that regularly visits the two marshes during spring and fall migrations and is highly dependent on freshwater invertebrates as the food resource. Fifteen Northern Shovelers were equipped with GPS/GSM tags and monitored during their stopover in both marshes in 2020 and 2021. The aims of the study were to understand the habitat use on stopover sites and relate home range (HR) size with characteristics of the feeding habitats (such as freshwater invertebrates’ density and diversity). The HR area of the studied individuals was mainly constituted of ponds in MB (83% of the HR) and wet meadows in MP (71% of the HR). The Northern Shovelers equipped with tag spent more than 72 consecutive hours in 31 wetlands, using them during the day, at night or all day. The diurnal visited sites were deep ponds that were sparsely vegetated and dominated by microcrustaceans, whereas the nocturnal visited sites were wet meadows or ponds with high aquatic vegetation cover and high invertebrate taxonomic diversity. The 31 described sites appeared to be rich in freshwater invertebrates, with no significant difference in invertebrate densities between the diurnal and nocturnal sites. HR sizes were highly homogenous between the two study sites (MB and MP), between sexes or between age classes. In conclusion, according to this study, an appropriate HR for the Northern Shoveler at spring stopover is 8.49 ± 5.95 km² (mean ± standard error).

Timing mowing for maximal energy gain - Managing foraging habitat of wintering geese under extreme drought conditions

The availability of high-quality food resources is a critical determinant of wildlife fitness. Over the past two decades, phenological mismatch - the temporal misalignment between animals’ peak nutrient demand and optimal resource availability - has emerged as a significant conservation challenge. This issue is particularly worrisome for migratory birds, which must accumulate energy reserves to meet the elevated metabolic demands of migration between breeding and wintering grounds. In Poyang Lake, a crucial wintering ground along the East Asian-Australasian flyway, increasing asynchrony between vegetation growth and the migration of herbivorous waterbirds significantly impedes conservation efforts and presents a major management challenge for this Ramsar wetland. This study evaluates the efficacy of mowing, a grassland management measure, in regulating plant growth processes and restoring food resources for geese. In-situ mowing experiments were conducted with varying timings in Carex wet meadows, the primary foraging habitats of geese. Optimal mowing times were identified, and the maximum goose carrying capacity was assessed by comparing Carex growth and nutritional dynamics with goose dietary requirements. The results reveal that mowing effectively slows down the aging process of Carex, and protein content is identified as a critical limiting factor for geese foraging. Different mowing timings extend the suitable foraging period by 11–25 days. Estimates suggest varying carrying capacities with different mowing timings, supporting goose populations ranging from 133 to 2,046 in Changhuchi Lake during wintering. The optimal mowing window is early October, avoiding dates before late September and after late November. Moreover, multiple-stage mowing is recommended to accommodate different wintering stages. The study highlights mowing as a potential habitat restoration approach for goose conservation, effectively mitigating the challenges imposed by phenological mismatch directly and indirectly caused by anthropogenic activities.

Consequences of Spiraea tomentosa invasion in Uropodina mite (Acari: Mesostigmata) communities in wet meadows

Vegetation cover has been consistently reported to be a factor influencing soil biota. Massive spreading of invasive plants may transform native plant communities, changing the quality of habitats as a result of modification of soil properties, most often having a directional effect on soil microorganisms and soil fauna. One of the most numerous microarthropods in the litter and soil is Acari. It has been shown that invasive plants usually have a negative effect on mites. We hypothesized that invasive Spiraea tomentosa affects the structure of the Uropodina community and that the abundance and species richness of Uropodina are lower in stands monodominated by S. tomentosa than in wet meadows free of this alien species. The research was carried out in wet meadows, where permanent plots were established in an invaded and uninvaded area of each meadow, soil samples were collected, soil moisture was determined and the mites were extracted. We found that Uropodina mite communities differed in the abundance of individual species but that the abundance and richness of species in their communities were similar. S. tomentosa invasion led primarily to changes in the quality of Uropodina communities, due to an increase in the shares of species from forest and hygrophilous habitats. Our results suggest that alien plant invasion does not always induce directional changes in mite assemblages, and conclude that the impact of an alien species on Uropodina may cause significant changes in the abundance and richness of individual species without causing significant changes in the abundance and diversity of their community.

De Novo Hybrid Assembly Unveils Multi-Chromosomal Mitochondrial Genomes in Ludwigia Species, Highlighting Genomic Recombination, Gene Transfer, and RNA Editing Events.

Biological invasions have been identified as the fifth cause of biodiversity loss, and their subsequent dispersal represents a major ecological challenge. The aquatic invasive species Ludwigia grandiflora subsp. hexapetala (Lgh) and Ludwigia peploides subsp. montevidensis (Lpm) are largely distributed in aquatic environments in North America and in Europe. However, they also present worrying terrestrial forms that are able to colonize wet meadows. To comprehend the mechanisms of the terrestrial adaptation of Lgh and Lpm, it is necessary to develop their genomic resources, which are currently poorly documented. We performed de novo assembly of the mitogenomes of Lgh and Lpm through hybrid assemblies, combining short reads (SR) and/or long reads (LR) before annotating both mitogenomes. We successfully assembled the mitogenomes of Lgh and Lpm into two circular molecules each, resulting in a combined total length of 711,578 bp and 722,518 bp, respectively. Notably, both the Lgh and Lpm molecules contained plastome-origin sequences, comprising 7.8% of the mitochondrial genome length. Additionally, we identified recombinations that were mediated by large repeats, suggesting the presence of multiple alternative conformations. In conclusion, our study presents the first high-quality mitogenomes of Lpm and Lgh, which are the only ones in the Myrtales order found as two circular molecules.

Phosphorus Interactions with Iron in Undisturbed and Disturbed Arctic Tundra Ecosystems.

Phosphorus (P) limitation often constrains biological processes in Arctic tundra ecosystems. Although adsorption to soil minerals may limit P bioavailability and export from soils into aquatic systems, the contribution of mineral phases to P retention in Arctic tundra is poorly understood. Our objective was to use X-ray absorption spectroscopy to characterize P speciation and associations with soil minerals along hillslope toposequences and in undisturbed and disturbed low-lying wet sedge tundra on the North Slope, AK. Biogenic mats comprised of short-range ordered iron (Fe) oxyhydroxides were prevalent in undisturbed wet sedge meadows. Upland soils and pond sediments impacted by gravel mining or thermokarst lacked biogenic Fe mats and were comparatively iron poor. Phosphorus was primarily contained in organic compounds in hillslope soils but associated with Fe(III) oxyhydroxides in undisturbed wet sedge meadows and calcium (Ca) in disturbed pond sediments. We infer that phosphate mobilized through organic decomposition binds to Fe(III) oxyhydroxides in wet sedge, but these associations are disrupted by physical disturbance that removes Fe mats. Increasing disturbances of the Arctic tundra may continue to alter the mineralogical composition of soils at terrestrial-aquatic interfaces and binding mechanisms that could inhibit or promote transport of bioavailable P from soils to aquatic ecosystems.

Distribution of plant species Iris sibirica and its vegetation affinity in Slovakia

Iris sibirica is a threatened plant species of the Central European flora, but its distribution and vegetation ecology in Slovakia have not been studied so far. Therefore, we aimed to compile a complex chorology of this species and to analyse phytosociological plots with the species occurrence. Our results suggest that Iris sibirica occurs almost in the whole Slovakia, with two distributional centres situated in the south-western region (Pannonian bioregion) and in the southern regions of central Slovakia (Carpathian bioregion), but a higher proportion of recent records was identified in the Carpathians. Vegetation classification using the TWINSPAN algorithm divided 80 vegetation plots into 5 clusters. They were interpreted syntaxonomically as follows: i) Calthion palustris alliance, ii) Deschampsion cespitosae alliance, iii) Molinion caeruleae alliance, iv) vegetation of the successional stages and transitional status between wet meadows (Calthion palustris and Molinion caeruleae) and fens (Scheuchzerio palustris-Caricetea fuscae), and v) vegetation with a transitional status between hygrophilous, fen vegetation of the alliances Molinion caeruleae, Caricion davallianae, Caricion fuscae and mesic habitats. Variation in the species composition of vegetation plots, analysed using detrended correspondence analysis (DCA) and interpreted using Ellenberg indicator values for vascular plants, showed a shift along the first DCA axis from taxa typical for nutrient-rich and shaded but thermophilic sites to taxa of open and relatively cold habitats on nutrient-poor soils. The second DCA axis corresponded to the moisture gradient, which controlled the pattern of plant species richness.

Using Relationships Between Vegetation and Surface Soil Biogeochemical Properties to Assess Regional Soil Carbon Inventories for South Baffin Island, Nunavut, Canada

AbstractAs Arctic regions warm rapidly, it is unclear whether high‐latitude soil carbon (C) will decrease or increase. Predicting future dynamics of Arctic soil C stocks requires a better understanding of the quantities and controls of soil C. We explore the relationship between vegetation and surface soil C in an understudied region of the Arctic: Baffin Island, Nunavut, Canada. We combined soil C data for three vegetation types—polar desert, mesic tundra, and wet meadow—with a vegetation classification to upscale soil C stocks. Surface soil C differed significantly across vegetation types, and interactions existed between vegetation type and soil depth. Polar desert soils were consistently mineral, with relatively thin organic layers, low percent C, and high bulk density. Mesic soils exhibited an organic‐rich epipedon overlying mineral soil. Wet meadows were consistently organic soil with low bulk density and high percent C. For the top 20 cm, polar desert contained the least soil C (2.17 ± 0.48 kg m−2); mesic tundra had intermediate C (8.92 ± 0.74 kg m−2); wet meadow stored the most C (13.07 ± 0.69 kg m−2). Extrapolating to the top 30 cm, our results suggest that approximately 44 Tg C is stored in the study region with a mean landscape soil C stock of 2.75 kg m−2 for non‐water areas. Combining vegetation mapping with local soil C stocks considerably narrows the range of estimates from other upscaling approaches (27–189 Tg) for soil C on South Baffin Island.

Excellent phenol removal ability of Acinetobacter guillouiae BY18 under different inorganic nitrogen conditions

Phenol and inorganic nitrogen generally exist in industrial wastewater and contaminated soil. In this study, Acinetobacter guillouiae BY18, which can simultaneously remove high concentrations of phenol and inorganic nitrogen, was isolated from a wet meadow in Weining County, Guizhou Province, China. The optimal remediation conditions for strain BY18 were 25 °C and pH 7.20–8.20, with a C/N ratio of 10, rotation speed of 150 r/min, and inoculation amount of 0.2×108 CFU. When phenol and ammonium were both present, strain BY18 could remove 982.86 mg/L of phenol and 64.76 mg/L of ammonium within 18 h. Strain BY18 was able to eliminate 979.71 mg/L, 55.84 mg/L, and 49.20 mg/L of phenol, nitrite, and nitrate, respectively, within 36 h when ammonium was substituted by nitrate or nitrite. In the simultaneous nitrification and denitrification reaction system, the maximum removal rates of phenol and ammonium were 92.53 mg/(L·h) and 5.7 mg/(L·h), respectively. Overall, strain BY18 had a remarkable phenol removal ability under different inorganic nitrogen conditions.

Effects of vegetation extreme degradation on soil hydrothermal processes in alpine wet meadow on the central Qinghai–Tibet Plateau

Ongoing climate warming and humidification have triggered a series of environmental responses, including vegetation succession, significant permafrost degradation, hydrological shifts, alterations in water resources, and increased frequency of freeze-thaw events. Notably, vegetation modulates the water cycle, regulates soil temperatures, and sustains permafrost stability. However, the extent to which the degradation of alpine vegetation impacts soil hydrothermal processes in permafrost regions is unclear. Therefore, we measured the soil moisture and temperature of the alpine wet meadow (AWM) and extremely degraded alpine wet meadow (EDAWM) ecosystems within the permafrost regions of the Qinghai–Tibet Plateau in situ. The objectives of this study were to explore the freeze-thaw cycles and hydrothermal dynamics within the active layer and to understand the mechanisms behind the effects of extreme alpine vegetation degradation. The results revealed that the AWM ecosystem had a longer soil freezing duration and a higher soil freezing rate than those of the EDAWM ecosystem. Additionally, the freezing index was higher in EDAWM than that in AWM, while differences in the thawing index were insignificant. The variance in the thaw-freeze ratios between the two ecosystems indicated that extreme vegetation degradation in AWM altered soil heat absorption and dissipation in the plant root zone and the deeper active layer. Moreover, EDAWM exhibited a decrease in soil bidirectional freezing processes, particularly from the permafrost table upwards. The extreme degradation in AWM changed soil physical properties and organic matter content, reducing ground temperatures in the active and permafrost layers of EDAWM, particularly during winter. The reduced heat transfer in EDAWM resulted in an active layer depth 9 cm shallower than that in AWM. Without vegetation cover, soil moisture in EDAWM was more prone to evaporation or deeper infiltration, leading to lower soil moisture content than that in AWM. Furthermore, an increase in soil moisture content decreased temperature in shallow soils in AWM but increased it in shallow soils in EDAWM. In summary, extreme vegetation degradation impaired air-heat exchange in AWM soil. These insights provide a scientific and theoretical basis for predicting permafrost evolution in the Qinghai–Tibet Plateau, highlighting the complex interactions among vegetation degradation, soil hydrothermal processes, and climatic factors.

Ecological Observations along the River Banks at Allahabad, U. P.

This paper embodies the ecological observations made along the exposed riverbeds and the immediate banks of the rivers Jamuna and Ganga at Allahabad during thé months of April to June in 1962 and 1963.The physical features, climate and soil of the area are discussed.Botanical composition of the vegetation and the relative abundance, preference for habitat, types, and phenology of the important species are given.The vegetation is characterised by the preponderance of annual plants and consists of the species of the wet meadow and dry meadow stages of Dudgeon (1920). The thorn scrub Stage is represented by Acacia arabica and Zizyphus jujuba.

Long‐term effects of meadow management on seed bank diversity and composition

AbstractAimsOligotrophic grasslands are habitats that host among the most diverse plant communities in Europe. Altering management regimes by either intensifying or ceasing management is known to decrease plant diversity. Yet, despite its importance for the recovery of plant communities after disturbances, little is known about whether seed banks are also affected by changes in management. Here, we investigate the effect of management practices on a meadow seed bank using a long‐term manipulative experiment. We focus on the response of the seed bank to the treatments, and the relationship between the seed bank and the vegetation response.MethodsThe study was conducted in a species‐rich wet meadow. The experiment consists of a factorial combination of fertilization, mowing, and removal of the dominant species. After 20 years of management, the seed bank was sampled seasonally at two soil layer depths. Standing vegetation was recorded in June at the peak of vegetation.ResultsAll seed bank characteristics varied between soil layers. Mowing decreased seed density and diversity, while fertilization significantly affected the species composition. Dominant removal had no effect on the seed bank. While seed bank diversity was not correlated to vegetation diversity, individual species' responses to mowing and fertilization were positively correlated in the seed bank and the vegetation.ConclusionsOur results show that long‐term management influences the seed bank down to 10 cm of soil depth. Whereas mowing apparently reduced seed density and diversity, the effects of fertilization on these characteristics were harder to interpret. After 20 years, most species had concordant responses to both mowing and fertilization, indicating a low legacy of previous management regimes on the seed bank. Our study reveals that the intensification of grassland management has a profound effect on plant diversity by directly affecting plant communities and their seed‐bank‐driven recovery potential.

Actual and reference evapotranspiration for a natural, temperate zone fen wetland – Upper Biebrza case study

Evapotranspiration is the key and predominant component of the water balance in wetlands. Direct evapotranspiration measurements are challenging in wetlands due to their remoteness and high surface water level. This article describes the actual (ETa and reference evapotranspiration (ET0) from a cultivated wet meadow located in the Biebrza National Park – the largest national park in north-east Poland, Central Europe. The data were sourced from a micrometeorological station equipped with an eddy covariance system to measure heat and vapour fluxes and such meteorological elements as radiation balance components, air temperature and humidity. The values of directly measured ETa were presented daily in the context of available energy and ET0. Daily sums of ETa ranged from below 0.2 mm in winter to 6.5 mm in summer. The share of daily sums of ETa in the ET0 usually ranged from 50 to 60%, with extreme values from 10 to 170%. Aside from giving more insight into Biebrza wetlands’ functioning, the actual data produced in this study may be used instead of indirect methods, which were used the most in modelling wetlands areas.

Topographic Drivers of Permafrost Organic Carbon Accumulation on the Northern Qinghai–Tibet Plateau

ABSTRACTLarge amounts of soil organic carbon (SOC) have accumulated over thousands of years in the northern permafrost regions. However, the SOC accumulation rates and the effects of topography, vegetation, and soil properties on SOC storage remain poorly constrained, leading to large uncertainties in the estimation of SOC storage and its response to warming. Here, we examined SOC stocks, densities, and accumulation rates using field sampling and radiometric dating in areas with different topographic parameters on the northern Qinghai–Tibet Plateau. Results show that SOC density and stock increases with elevation on south‐facing slopes but decreases with elevation on north‐facing slopes. The carbon accumulation rates in the upper 6 m soil are highest (16.32 g C m−2 year−1) in the north‐facing alpine wet meadow and lowest (1.07 g C m−2 year−1) in the south‐facing alpine steppe. Elevation‐, slope‐, and slope aspect‐induced variations in soil properties and vegetation type are the main factors controlling the spatial SOC distribution. These results imply that the topography strongly affects the spatial heterogeneity of SOC in mountainous permafrost regions. Overall, our findings highlight that topographic factors should be further integrated into carbon models of carbon responses to climate change in permafrost‐affected soils.

Response of species dominance and niche of plant community to wetland degradation along alpine lake riparian.

Alpine wetland degradation threatens riparian biodiversity and ecological balance. Our study, conducted in July 2020 along the northern and eastern shores of Qinghai Lake, seeks to unravel the impacts of such degradation on plant species dominance and ecological niches, using advanced network analysis methods to explore the dynamics and survival strategies of plant species. We applied a space-to-time method to delineate three wetland degradation stage: a healthy swamp wetland, a slightly degraded wet meadow, and a degraded dry meadow. Six representative sampling points were chosen. At each point, three sample lines were randomly established, radiating outward from the center of the lake wetland, with each stage of degradation meticulously examined through three replicates to assess the plant communities in terms of species composition, plant height, coverage, and abundance. The results indicated: Species such as Kobresia tibetica and Leymus secalinus exhibit remarkable abundance across various stages of wetland degradation, indicating a robust tolerance to these conditions. This observation, coupled with the complexity of plant community structures in degrading wetlands, suggests that such intricacy cannot be solely attributed to the dominance of particular species. Instead, it is the result of a diverse array of species adapting to fluctuating water levels, which promotes increased species richness. Despite the prominence of species that exhibit rapid growth and reproduction, the ecological significance of less abundant species in contributing to the community's complexity is also notable. Changes in habitat conditions due to wetland degradation facilitate both competitive and cooperative interactions among species, highlighting the dynamic nature of these ecosystems. Our analysis shows no significant linear relationship between the ecological niche overlap values and niche widths of plant species. However, the strategies employed by dominant species for competition and resource acquisition, as observed in the ecological niche overlap networks, underscore the adaptive capacity of plant communities. These insights underscore the need for tailored restoration strategies to conserve the biodiversity of alpine lake riparian ecosystems. This research not only sheds light on the resilience and adaptability of ecosystems in the Qinghai-Tibetan Plateau but also offers valuable lessons for the conservation of similar habitats worldwide. Our findings underscore the need for tailored restoration strategies to conserve the biodiversity of alpine lake riparian ecosystems. This research not only sheds light on the resilience and adaptability of ecosystems in the Qinghai-Tibetan Plateau but also offers valuable lessons for the conservation of similar habitats worldwide.

Měkkýši národní přírodní rezervace Jazevčí v CHKO Bílé Karpaty [Molluscs of Jazevčí National Nature Reserve in the White Carpathians PLA

The Jazevčí National Nature Reserve is located in the southern part of the White Carpathians PLA near the Javorník nad Veličkou village. The reserve protects a heterogeneous landscape with meadows, groves, springs, and creeks. This diversity is reflected in the mollusc assemblages, which contain nine out of ten ecological groups. In total, 58 mollusc species are known from the reserve, 56 being terrestrial gastropods with one aquatic snail and one bivalve. Most diversity consists of forest species, followed by the euryvalent and hygrophilous species. Three species are listed in the Red List of Threatened Species: Daudebardia brevipes (VU), Bythinella austriaca (NT), and Vertigo angustior (VU), the latter also being internationally protected under Annex II of the EU Habitats Directive. Vertigo angustior was found in a deciduous forest, a habitat highly unusual for this species. Trochulus villosulus, a rare species, was found in a wet meadow near the Velička stream. As most species diversity is found in groves and forests, a non-intervention regime is suggested to retain their species diversity, while meadows should be managed extensively by kettle grazing or mowing.

Mapping wing morphs of Tetrix subulata using citizen science data: Flightless groundhoppers are more prevalent in grasslands near water

Abstract The slender groundhopper (Orthoptera: Tetrigidae) Tetrix subulata (Linnaeus, 1758), like several other taxa of winged insects, has a long‐winged and a short‐winged form, the latter incapable of flight. The optimal habitat of T. subulata consists of wet meadows and flat shores, as they feed mostly on algae and mosses in damp, sun‐exposed, open patches. Contrasting hypotheses predict either short‐ or long‐winged morphs to be relatively more prevalent in optimal habitats. Previous investigations were limited in their spatial extent. To strongly increase the number of study locations, we obtained, through the Global Biodiversity Information Facility (GBIF), geo‐referenced observations made by citizen scientists in the Netherlands. We determined individuals' wing form based on photographs accompanying these observations and related wing‐form patterns to large‐ and medium‐scale landscape characteristics. Our analyses revealed that in grasslands near the major rivers, relatively more short‐winged individuals were found. In drier landscapes consisting of coniferous forests and heath on the higher sand areas, more long‐winged individuals were observed. Built‐up areas had no significant effect on the wing length. These patterns might be caused by the oogenesis‐flight syndrome or small‐scale spatial sorting, but the exact mechanism is yet unknown, as size‐dependency might also play a role. Although opportunistic observations are biased by where and when observers prefer to go, and by reporting probabilities varying between species groups, these biases are not likely to have affected our conclusions. Citizen science data appear to be suitable for analyses of spatial distribution and sorting of morphological types within species.

Mowing wet meadows reduces the health of their snail communities.

Wet meadows harbor rich biodiversity, making them pivotal ecosystems worldwide. These habitats are commonly used for grazing or hay production for livestock. However, regular mowing can influence these habitats, potentially leading to significant repercussions for the animals residing within them. In order to investigate the effects of land management practices, we conducted an experimental study to compare snail communities in mowed and unmowed wet meadows in northern Hungary. We found that overall, mowing decreases snail populations, as well as species richness and diversity. Thus, our results suggest that routine mowing of wet meadows is deleterious to their snail communities. Based on these results, we suggest that designated patches of meadows that are regularly managed should be left uncultivated in their natural state. These patches can serve as potential colonization sites, facilitating the restoration of the entire meadow's ecological balance.

Vicuña antipredator diel movement drives spatial nutrient subsidies in a high Andean ecosystem.

Large animals could be important drivers of spatial nutrient subsidies when they ingest resources in some habitats and release them in others, even moving nutrients against elevational gradients. In high Andean deserts, vicuñas (Vicugna vicugna) move daily between nutrient-rich wet meadows, where there is abundant water and forage but high risk of predation by pumas (Puma concolor), and nutrient-poor open plains with lower risk of predation. In all habitats, vicuñas defecate and urinate in communal latrines. We investigated how these latrines impacted soil and plant nutrient concentrations across three habitats in the Andean ecosystem (meadows, plains, and canyons) and used stable isotope analysis to explore the source of fecal nutrients in latrines. Latrine soils had higher concentrations of nitrogen, carbon, and other nutrients than did nonlatrine soils across all habitats. These inputs corresponded with an increase in plant quality (lower C:N) at latrine sites in plains and canyons, but not in meadows. Stable isotope mixing models suggest that ~7% of nutrients in plains latrines originated from vegetation in meadows, which is disproportionately higher than the relative proportion of meadow habitat (2.6%) in the study area. In contrast, ~68% of nutrients in meadow latrines appear to originate from plains and canyon vegetation, though these habitats made up nearly 98% of the study area. Vicuña diel movements thus appear to concentrate nutrients in latrines within habitats and to drive cross-habitat nutrient subsidies, with disproportionate transport from low-lying, nutrient-rich meadows to more elevated, nutrient-poor plains. When these results are scaled up to the landscape scale, the amount of nitrogen and phosphorus subsidized in soil at plains latrines was of the same order of magnitude as estimates of annual atmospheric nitrogen and phosphorus deposition for this region (albeit far more localized and patchy). Thus, vicuña-mediated nutrient redistribution and deposition appears to be an important process impacting ecosystem functioning in arid Andean environments, on par with other major inputs of nutrients to the system.

Flowering phenology differs among wet and dry sub-alpine meadows in southwestern China.

The effect of floral traits, floral rewards and plant water availability on plant-pollinator interactions are well-documented; however, empirical evidence of their impact on flowering phenology in high-elevation meadows remains scarce. In this study, we assessed three levels of flowering phenology, i.e. population-, individual- and flower-level (floral longevity), in two nearby but contrasting (wet versus dry) sub-alpine meadows on Yulong Snow Mountain, southwestern China. We also measured a series of floral traits (pollen number, ovule number, and the ratio of pollen to ovule number per flower, i.e. pollen:ovule ratio [P/O]) and floral rewards (nectar availability and pollen presentation) as plausible additional sources of variation for each phenological level. Floral longevity in the wet meadow was significantly longer than that for the dry meadow, whereas population- and individual-flowering duration were significantly shorter. Our results showed a significant positive relationship between flowering phenology with pollen number and P/O per flower; there was no relationship with ovule number per flower. Further, we found a significant effect of flowering phenology on nectar availability and pollen presentation. Our findings suggest that shorter floral longevity in dry habitats compared to wet might be due to water-dependent maintenance costs of flowers, where the population- and individual-level flowering phenology may be less affected by habitats. Our study shows how different levels of flowering phenology underscore the plausible effects of contrasting habitats on reproductive success.