An assemblage of cylindrical trace fossils that occur in the Late Jurassic Missão Velha Formation is presented and interpreted to be the casts of lungfish burrows. These small-sized casts are interpreted as passive siliciclastic sedimentation into abandoned excavations made by lungfish, suggesting their presence in the Missão Velha Formation paleofauna. The identification is based on their morphological similarity to previously identified and extant lungfish burrows. The cross-section of the burrows is very similar to the modern burrow morphology of the African lungfish Protopterus along its full vertical length, indicating lungfish aestivation, and supplemented by spatial clustering reflecting gregarious behavior. The burrow fillings occur in siltstone lithofacies intercalated with cross-stratified fluvial sandstones. These strata were deposited in a continental environment that includes braided fluvial channels, floodplains, and paleosols. Lungfish burrows have been used to infer the presence of seasonal droughts in the geologic past, and the occurrence of lungfish burrows in the Missão Velha Formation adds new insights into the Late Jurassic climate of the Araripe Basin. This climate provided sufficient moisture punctuated by seasonally dry periods, affording a habitat that supported lungfish aestivation behavior.