Well-defined Sites Research Articles

Cyberknife treatment for different types of tumor

CyberKnife radiosurgery is a frameless, image guided robotic stereotactic radiosurgical system (IGR-SRS) which uses potent, ablative radiation dose with limited treatment volume. The main aim of this study is to provide knowledge of this treatment for different types of tumor. To deliver radiation treatment from multiple angles, with real time target tracking through a combined orthogonal radiograph imaging and optic motion tracking system cyberKnife is a new and advanced technique. Radiation is delivered to the tumor within a predetermined range, for this an object recognition computer algorithm and a fudicial marker placed within a tumor. CyberKnife treatment cost USD 6,800 (Indian Rupees approx. 4,89,000). In this review you will get idea about different types of tumor get treated with cyberKnife radiosurgery (SRS). CyberKnife have submillimeter accuracy in metastatic brain tumor. By giving appropriate tracking method for stage I lung cancer and lung oligometastases stereotactic radiotherapy is an efficient treatment. So, at well-defined tumor sites cyberKnife is a new stereotactic body radiation therapy which delivers high dose of radiation in short period of time. For head and neck tumor treatment cyberKnife is most widely used. This treatment is helpful for adrenal gland tumor and prostate tumor, spinal metastases.

Pyrolysis-Free Synthesis of Bimetal Phthalocyanine Covalent Organic Polymers/Ordered Mesoporous Carbon Nanocomposites for an Efficient Oxygen Reduction Reaction

The development of non-noble metal–nitrogen ring complex (M–N–C) catalysts with high activity and stability for the oxygen reduction reaction (ORR) is of great significance. However, M–N–C catalysts are generally prepared from precursors under high-temperature pyrolysis, which leads to complex product structures and makes it difficult to identify the catalytic active center. Herein, we have successfully structured a novel bimetal phthalocyanine covalent organic polymer/ordered mesoporous carbon (CoFe-COP/OMC) electrocatalyst through a pyrolysis-free approach. Due to the well-defined active sites (Fe/CoN4), ordered COP structure, and highly conductive carrier materials, the CoFe-COP/OMC nanocomposite exhibits remarkable ORR electrocatalytic activity with a half-wave potential and initial potential of 0.908 and 0.932 V (vs reversible hydrogen electrode (RHE)), respectively, a limiting current density of 5.35 mA cm–2, and a nearly four-electron reduction pathway. In addition, the acquired hybrid catalysts show excellent methanol resistance and electrochemical stability compared with Pt/C. The outstanding performance confirms that the CoFe-COP/OMC nanocomposite is a promising high-efficiency ORR catalyst for metal–air batteries and fuel cells.

Expression of Secretin and its Receptor Along the Intestinal Tract in Type 2 Diabetes Patients and Healthy Controls.

The hormone secretin (SCT) is released from intestinal S cells and acts via the SCT receptor (SCTR). Circulating SCT levels increase after Roux-en-Y gastric bypass surgery and have been associated with massive weight loss and high remission rates of type 2 diabetes (T2D) linked to these operations. Exogenous SCT was recently shown to reduce ad libitum food intake in healthy volunteers. To understand SCT biology and its potential role in T2D pathophysiology, we examined the intestinal mucosal expression profile of SCT and SCTR and evaluated the density of S cells along the intestinal tract of individuals with T2D and healthy controls. Using immunohistochemistry and messenger RNA (mRNA) sequencing, we analyzed intestinal mucosa biopsies sampled along the small intestine at 30-cm intervals and from 7 well-defined anatomical sites along the large intestine (during 2 sessions of double-balloon enteroscopy) in 12 individuals with T2D and 12 healthy controls. Both groups exhibited a progressive and similar decrease in SCT and SCTR mRNA expression and S-cell density along the small intestine, with reductions of 14, 100, and 50 times, respectively, in the ileum compared to the duodenum (used as reference). Negligible amounts of SCTR and SCT mRNA, as well as low S-cell density, were found in the large intestine. No significant differences were observed between the groups. SCT and SCTR mRNA expression and S-cell density were abundant in the duodenum and decreased along the small intestine. Very low SCT and SCTR mRNA levels and S-cell numbers were observed in the large intestine, without aberrations in individuals with T2D compared to healthy controls.

Electrochemical Synthesis of Nanostructured Ordered Intermetallic Materials under Ambient Conditions.

ConspectusThe enhanced catalytic properties of alloy nanostructures have made them a focus of extensive research in the field of catalysis. Alloy nanostructures can be classified into two types: disordered alloys (also known as solid solutions) and ordered intermetallics. The latter are of particular interest as they possess long-range atomic scale ordering, which leads to well-defined active sites that can be used to accurately assess structure-property relationships and their impact on (electro)catalytic performance.While many ordered intermetallics (OICs) have been synthesized and evaluated as electrocatalysts, there is still a lack of understanding on how the local structure of atoms controls their catalytic performance. Ordered intermetallics are difficult to synthesize and often require high-temperature annealing for the atoms to equilibrate into ordered structures. High temperature processing results in aggregated structures (usually >30 nm) and/or contamination from the support, which can decrease their performance and preclude these materials from being used as model systems for elucidating insight into structure and electrochemical properties. Therefore, alternative methods are required to enable more efficient atomic ordering while maintaining some level of morphological control.This Account delves into the potential of electrochemical methods as a practical alternative for synthesizing ordered intermetallics at lower temperatures. Specifically, it explores the viability of electrochemical dealloying and electrochemical deposition to synthesize Pd-Bi and Cu-Zn intermetallics at room temperature and atmospheric pressure. These methods have proven useful in synthesizing phases that are typically inaccessible under ambient conditions. The high homologous temperatures at which these materials are synthesized provide the necessary atomic mobility required for equilibration and formation of ordered phases, thus making the direct synthesis of ordered intermetallic materials at room temperature by electrochemical means a reality.Beyond synthesis, the electrocatalytic performance of these intermetallics was assessed for the oxygen reduction reaction (ORR), which is an important process employed in fuel cells. The OICs displayed increased performance with respect to commercial Pd/C and Pt/C benchmarks because of lower coverages of spectator species. Furthermore, these materials exhibited improved methanol tolerance.This Account provides valuable insights into the electrochemical synthesis of ordered intermetallics and their potential use as highly effective catalysts for electrocatalytic reactions. By using electrochemical methods, it is possible to obtain ordered intermetallics with unique atomic arrangements and tailored properties, which can be optimized for specific catalytic applications. With further research, electrochemical synthesis methods may enable the development of new and improved ordered intermetallics with even higher catalytic activity and selectivity, making them ideal candidates for use in a wide range of industrial processes. Furthermore, the ability to access intermetallics under milder conditions may accelerate the ability to use these materials as model systems for revealing fundamental insight into electrocatalyst structure and function.

Isolated Single-Atom Cobalt in the ZnIn2S4 Monolayer with Exposed Zn Sites for CO2 Photofixation

Constructing highly active photocatalysts with rich reactive sites for photofixation of CO2 with organic compounds implements an environmentally friendly route for CO2 valorization and carbon neutralization. Herein, we report the isolated cobalt (Co) single-atom (SA)-decorated two-dimensional (2D) single-layer ZnIn2S4 (ZIS) nanosheet composites (Co-sZIS) with dual active centers of Co and Zn for CO2 fixation with epoxides under visible light. The unique ensemble of Co-sZIS optimizes light harvesting, promotes charge carrier separation, and enriches the bifunctional active sites for the efficient adsorption and activation of the reactants of epoxides and CO2. Consequently, the Co-sZIS exhibits significantly improved CO2-epoxide cycloaddition performance compared to bulk ZIS. In addition, the synergetic photoredox manner of simultaneously exploiting photogenerated electrons and holes efficiently facilitates the activation of CO2 and epoxides, thereby further reducing the energy barrier of CO2 fixation. This work not only provides a paradigm of rationally interfacial engineered SA-onto-2D semiconductors for CO2 valorization but also highlights the structure–activity relationship by the well-defined active sites in this SA-based hybrid platform.

Electronic and Coordination Effect of PtPd Nanoflower Alloys for the Methanol Electrooxidation Reaction

Platinum-based electrocatalysts usually exhibit limited and quickly vanished activity for the methanol oxidation reaction (MOR) due to active site occupation and surface intermediate poisoning, especially CO. The attendant synergistic effect of the alloy strategy is often executed to improve these flaws. Here, PtPd nanoflower alloys uniformly anchored on the carbon paper were successfully synthesized by electrodeposition to construct well-defined active sites, over which high MOR activity and tolerance were realized. The homogeneous alloy structure of PtPd nanoflowers was confirmed by TEM and XAFS. Impressively, the Pt1Pd1-N NFs/CP exhibited an excellent mass activity of 9.25 A mgPt+Pd–1 and specific activity of 13.2 mA cmECSA–2 of MOR in the alkaline electrolyte, and 87.8% of the initial activity was still retained after 500 cycles. In situ infrared spectroscopy revealed that Pt1Pd1-N NFs/CP ensured the complete oxidation of methanol following a CO-free path. The investigation of the electronic and coordination structure demonstrated that the Pt–Pd adjacent sites with a zero valence are the highly active center for MOR, and the synergistic effect between Pt and Pd greatly improved the MOR electrocatalytic performance. This work offers an effective strategy to precisely control the number and structure of decorating species for constructing alloy nanocatalysts to enhance the electroactivity toward MOR.

Cure-PROs: Next-generation targeted protein degraders.

e15101 Background: Many proteins, including transcription factors and scaffolding proteins, are not amenable to targeting by traditional small molecule inhibitors due to the lack of a well-defined binding pocket or active site. Proteolysis-Targeting Chimeras (PROTACs) are a new class of hetero-bifunctional molecules that bind both a target protein and an E3 ubiquitin ligase, bringing the two into proximity for appending ubiquitin, and subsequently marking the target protein for proteasomal degradation. Currently, thirteen PROTACs are in clinical trials for oncology indications. However, the clinical utility of PROTACs is challenged by their large size and long development timelines. Also, resistance mutations in the E3 ligase or transporter overexpression inevitably evolve. Thus, a new platform for small-molecule degraders that enables ultra-rapid drug development timelines, efficient cellular uptake, and can be developed to overcome innate and acquired drug resistance is needed. Methods: Coferons, developed in our laboratory, are small molecules that self-assemble upon binding to a target, where they form reversible covalent dimers through bio-orthogonal linker chemistries. We have combined features of the Coferon platform and PROTACs to generate CURE-PROs (Combinatorial Ubiquitination REal-time PROteolysis), consisting of one target protein ligand and one E3 ligase ligand that form reversible heterodimers that lead to targeted protein degradation within cells. By modifying known ligands for BRD4, and the E3 ubiquitin ligases Cereblon, VHL, and MDM2, with linkers able to reversibly join the BRD4 to the ligase ligands, we synthesized libraries of CURE-PRO monomers that can be combined to create thousands of CURE-PRO dimer combinations. We explored whether this platform could yield meaningful BRD4 degradation in vitro and in vivo. Results: Rapid combinatorial cell-based screening identified several BRD4-E3 ligase CURE-PRO combinations that induced greater than 50% BRD4 degradation, with the most promising CURE-PRO pairs achieving more than 95% protein degradation. Consistent with a PROTAC mechanism-of-action, successful CURE-PRO combinations confirmed significant protein degradation which was inhibited by proteasome inhibitors or competition with parent ligands. Significant BRD4 degradation was also observed in mice bearing bilateral human xenograft tumors, confirming CURE-PRO proof-of-mechanism in vivo. Conclusions: The combinatorial nature of our platform has the potential to significantly reduce synthesis time and effort to identify the optimal linker length and E3 ligase for each target protein. The CURE-PRO platform consists of expanding libraries of monomers for both additional oncoprotein targets as well as E3 ligases, which can be redeployed to shorten lead development timelines.

Precisely manipulated π–π stacking of catalytic exfoliated iron polyphthalocyanine/reduced graphene oxide hybrid via pyrolysis-free path

Metal macrocycles with well-defined molecular structures are ideal platforms for the in-depth study of electrochemical oxygen reduction reaction (ORR). Structural integrity of metal macrocycles is vital but remain challenging since the commonly used high-temperature pyrolysis would cause severe structure damage and unidentifiable active sites. Herein, we propose a pyrolysis-free strategy to precisely manipulate the exfoliated 2D iron polyphthalocyanine (FePPc) anchored on reduced graphene oxide (rGO) via π–π stacking using facile high-energy ball milling. A delocalized electron shift caused by π–π interaction is firstly found to be the mechanism of facilitating the remarkable ORR activity of this hybrid catalyst. The optimal FePPc@rGO-HE achieves superior half-wave potential (0.90 V) than 20 % Pt/C. This study offers a new insight in designing stable and high-performance metal macrocycle catalysts with well-defined active sites.

The Many Ways to Deal with STING.

The stimulator of interferon genes (STING) is an adaptor protein involved in the activation of IFN-β and many other genes associated with the immune response activation in vertebrates. STING induction has gained attention from different angles such as the potential to trigger an early immune response against different signs of infection and cell damage, or to be used as an adjuvant in cancer immune treatments. Pharmacological control of aberrant STING activation can be used to mitigate the pathology of some autoimmune diseases. The STING structure has a well-defined ligand binding site that can harbor natural ligands such as specific purine cyclic di-nucleotides (CDN). In addition to a canonical stimulation by CDNs, other non-canonical stimuli have also been described, whose exact mechanism has not been well defined. Understanding the molecular insights underlying the activation of STING is important to realize the different angles that need to be considered when designing new STING-binding molecules as therapeutic drugs since STING acts as a versatile platform for immune modulators. This review analyzes the different determinants of STING regulation from the structural, molecular, and cell biology points of view.

UiO-66 framework equipped with cadmium-thiocatecholato moieties as photocatalyst bearing stable active sites and intrinsic photosensitizing units for hydrogen evolution reaction

Photocatalytic hydrogen evolution reaction (HER) represents one of the most promising technologies for sustainable development. Even though metal-organic framework (MOF), comprising rich topologies and tunable functionalities, is getting attention as a new generation of photocatalyst, a majority of them only provide unclear active sites along with the use of external noble-metal based photosensitizers and environmentally unfriendly scavengers. Therefore, it is urged to develop MOFs possessing structurally unambiguous active sites along with inherent photosensitizing units and execute photocatalytic HER with greener sacrificial reagents. Herein, we report a UiO-66-type framework, namely UiO-66-dcbdt-Cd (dcbdt2− = 1,4-dicarboxylatebenzene-2,3-dithiol), bearing Cd-thiocatecholato moieties as intrinsic photosensitizing units and structurally well-defined active sites for photocatalytic HER in H2O. UiO-66-dcbdt-Cd gave the best HER yield of 5.29 mmol g−1 and rate of 1.32 mmol g−1 h−1, outperforming the negligible HER performance of pristine metal-free UiO-66-dcbdt. This work provides insight to manipulation of thiocatecholate functionalities inside MOFs to construct inherent photosensitizing units as well as stable structurally unambiguous active sites for sustainable photocatalytic applications.

A pyrolysis-free Ni/Fe bimetallic electrocatalyst for overall water splitting

Catalysts capable of electrochemical overall water splitting in acidic, neutral, and alkaline solution are important materials. This work develops bifunctional catalysts with single atom active sites through a pyrolysis-free route. Starting with a conjugated framework containing Fe sites, the addition of Ni atoms is used to weaken the adsorption of electrochemically generated intermediates, thus leading to more optimized energy level sand enhanced catalytic performance. The pyrolysis-free synthesis also ensured the formation of well-defined active sites within the framework structure, providing ideal platforms to understand the catalytic processes. The as-prepared catalyst exhibits efficient catalytic capability for electrochemical water splitting in both acidic and alkaline electrolytes. At a current density of 10 mA cm−2, the overpotential for hydrogen evolution and oxygen evolution is 23/201 mV and 42/194 mV in 0.5 M H2SO4 and 1 M KOH, respectively. Our work not only develops a route towards efficient catalysts applicable across a wide range of pH values, it also provides a successful showcase of a model catalyst for in-depth mechanistic insight into electrochemical water splitting.

Hydrophobicity Tailoring of Ferric Covalent Organic Framework/MXene Nanosheets for High-Efficiency Nitrogen Electroreduction to Ammonia.

Electrocatalytic nitrogen reduction reaction (NRR) represents a promising sustainable approach for NH3 synthesis. However, the poor NRR performance of electrocatalysts is a great challenge at this stage, mainly owing to their low activity and the competitive hydrogen evolution reaction (HER). Herein, 2D ferric covalent organic framework/MXene (COF-Fe/MXene) nanosheets with controllable hydrophobic behaviors are successfully prepared via a multiple-in-one synthetic strategy. The boosting hydrophobicity of COF-Fe/MXene can effectively repel water molecules to inhibit the HER for enhanced NRR performances. By virtue of the ultrathin nanostructure, well-defined single Fe sites, nitrogen enrichment effect, and high hydrophobicity, the 1H,1H,2H,2H-perfluorodecanethiol modified COF-Fe/MXene hybrid shows a NH3 yield of 41.8µg h-1 mgcat. -1 and a Faradaic efficiency of 43.1% at -0.5V versus RHE in a 0.1m Na2 SO4 water solution, which are vastly superior to the known Fe-based catalysts and even to the noble metal catalysts. This work provides a universal strategy to design and synthesis of non-precious metal electrocatalysts for high-efficiency N2 reduction to NH3 .

Rational design of heterogenized molecular phthalocyanine hybrid single-atom electrocatalyst towards two-electron oxygen reduction

Single-atom catalysts supported on solid substrates have inspired extensive interest, but the rational design of high-efficiency single-atom catalysts is still plagued by ambiguous structure determination of active sites and its local support effect. Here, we report hybrid single-atom catalysts by an axial coordination linkage of molecular cobalt phthalocyanine with carbon nanotubes for selective oxygen reduction reaction by screening from a series of metal phthalocyanines via preferential density-functional theory calculations. Different from conventional heterogeneous single-atom catalysts, the hybrid single-atom catalysts are proven to facilitate rational screening of target catalysts as well as understanding of its underlying oxygen reduction reaction mechanism due to its well-defined active site structure and clear coordination linkage in the hybrid single-atom catalysts. Consequently, the optimized Co hybrid single-atom catalysts exhibit improved 2e− oxygen reduction reaction performance compared to the corresponding homogeneous molecular catalyst in terms of activity and selectivity. When prepared as an air cathode in an air-breathing flow cell device, the optimized hybrid catalysts enable the oxygen reduction reaction at 300 mA cm−2 exhibiting a stable Faradaic efficiency exceeding 90% for 25 h.

A Priori Design of Dual-Atom Alloy Sites and Experimental Demonstration of Ethanol Dehydrogenation and Dehydration on PtCrAg.

Single-atom catalysts have received significant attention for their ability to enable highly selective reactions. However, many reactions require more than one adjacent site to align reactants or break specific bonds. For example, breaking a C-O or O-H bond may be facilitated by a dual site containing an oxophilic element and a carbophilic or "hydrogenphilic" element that binds each molecular fragment. However, design of stable and well-defined dual-atom sites with desirable reactivity is difficult due to the complexity of multicomponent catalytic surfaces. Here, we describe a new type of dual-atom system, trimetallic dual-atom alloys, which were designed via computation of the alloying energetics. Through a broad computational screening we discovered that Pt-Cr dimers embedded in Ag(111) can be formed by virtue of the negative mixing enthalpy of Pt and Cr in Ag and the favorable interaction between Pt and Cr. These dual-atom alloy sites were then realized experimentally through surface science experiments that enabled the active sites to be imaged and their reactivity related to their atomic-scale structure. Specifically, Pt-Cr sites in Ag(111) can convert ethanol, whereas PtAg and CrAg are unreactive toward ethanol. Calculations show that the oxophilic Cr atom and the hydrogenphilic Pt atom act synergistically to break the O-H bond. Furthermore, ensembles with more than one Cr atom, present at higher dopant loadings, produce ethylene. Our calculations have identified many other thermodynamically favorable dual-atom alloy sites, and hence this work highlights a new class of materials that should offer new and useful chemical reactivity beyond the single-atom paradigm.

Ligand vacancy channels in pillared inorganic-organic hybrids for electrocatalytic organic oxidation with enzyme-like activities

Simultaneously achieving abundant and well-defined active sites with high selectivity has been one of the ultimate goals for heterogeneous catalysis. Herein, we construct a class of Ni hydroxychloride-based inorganic-organic hybrid electrocatalysts with the inorganic Ni hydroxychloride chains pillared by the bidentate N-N ligands. The precise evacuation of N-N ligands under ultrahigh-vacuum forms ligand vacancies while partially retaining some ligands as structural pillars. The high density of ligand vacancies forms the active vacancy channel with abundant and highly-accessible undercoordinated Ni sites, exhibiting 5-25 fold and 20-400 fold activity enhancement compared to the hybrid pre-catalyst and standard β-Ni(OH)2 for the electrochemical oxidation of 25 different organic substrates, respectively. The tunable N-N ligand can also tailor the sizes of the vacancy channels to significantly impact the substrate configuration leading to unprecedented substrate-dependent reactivities on hydroxide/oxide catalysts. This approach bridges heterogenous and homogeneous catalysis for creating efficient and functional catalysis with enzyme-like properties.

Revealing substrate-induced structural changes in active site of human CYP51 in the presence of its physiological substrates

The human sterol 14α-demethylases (CYP51, CYP is an abbreviation for cytochrome P450) catalyze three-step oxidative removal of 14α-methyl group of lanosterol by first forming an alcohol, then an aldehyde, and finally conducting a CC bond cleavage reaction. This present study utilizes a combination of Resonance Raman spectroscopy and Nanodisc technology to probe the active site structure of CYP51 in the presence of its hydroxylase and lyase substrates. Ligand-binding induced partial low-to-high-spin conversion is observed by applying electronic absorption spectroscopy and Resonance Raman (RR) spectroscopy. This low degree of spin conversion of CYP51 is contributed by the retention of the water ligand coordinated to the heme iron as well as direct interaction between the hydroxyl group of lyase substrate and the iron center. No significant changes in active site structure are found between detergent-stabilized CYP51 and nanodisc-incorporated CYP51, nevertheless, it is demonstrated that nanodisc-incorporated assemblies provide much more well-defined active site RR spectroscopic responses, which induces a larger conversion from low-to-high-spin state in presence of the substrates. Moreover, a positive polar environment around the exogenous diatomic ligand is detected, providing insight into the mechanism of this essential CC bond cleavage reaction.

Conceptual and Practical Aspects of Metal-Organic Frameworks for Solid-Gas Reactions.

The presence of site-isolated and well-defined metal sites has enabled the use of metal-organic frameworks (MOFs) as catalysts that can be rationally modulated. Because MOFs can be addressed and manipulated through molecular synthetic pathways, they are chemically similar to molecular catalysts. They are, nevertheless, solid-state materials and therefore can be thought of as privileged solid molecular catalysts that excel in applications involving gas-phase reactions. This contrasts with homogeneous catalysts, which are overwhelmingly used in the solution phase. Herein, we review theories dictating gas phase reactivity within porous solids and discuss key catalytic gas-solid reactions. We further treat theoretical aspects of diffusion within confined pores, the enrichment of adsorbates, the types of solvation spheres that a MOF might impart on adsorbates, definitions of acidity/basicity in the absence of solvent, the stabilization of reactive intermediates, and the generation and characterization of defect sites. The key catalytic reactions we discuss broadly include reductive reactions (olefin hydrogenation, semihydrogenation, and selective catalytic reduction), oxidative reactions (oxygenation of hydrocarbons, oxidative dehydrogenation, and carbon monoxide oxidation), and C-C bond forming reactions (olefin dimerization/polymerization, isomerization, and carbonylation reactions).

Liquid Nitrogen Sources Assisting Gram-Scale Production of Single-Atom Catalysts for Electrochemical Carbon Dioxide Reduction.

Developing metal-nitrogen-carbon (M-N-C)-based single-atom electrocatalysts for carbon dioxide reduction reaction (CO2 RR) have captured widespread interest because of their outstanding activity and selectivity. Yet, the loss of nitrogen sources during the synthetic process hinders their further development. Herein, an effective strategy using 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4 ]) as a liquid nitrogen source to construct a nickel single-atom electrocatalyst (Ni-SA) with well-defined Ni-N4 sites on a carbon support (denoted as Ni-SA-BB/C) is reported. This is shown to deliver a carbon monoxide faradaic efficiency of >95% over a potential of -0.7 to -1.1V (vs reversible hydrogen electrode) with excellent durability. Furthermore, the obtained Ni-SA-BB/C catalyst possesses higher nitrogen content than the Ni-SA catalyst prepared by conventional nitrogen sources. Importantly, only thimbleful Ni nanoparticles (Ni-NP) are contained in the large-scale-prepared Ni-SA-BB/C catalyst without acid leaching, and with only a slight decrease in the catalytic activity. Density functional theory calculations indicate a salient difference between Ni-SA and Ni-NP in the catalytic performance toward CO2 RR. This work introduces a simple and amenable manufacturing strategy to large-scale fabrication of nickel single-atom electrocatalysts for CO2 -to-CO conversion.

Understanding Synergistic Catalysis on Cu-Se Dual Atom Sites via Operando X-ray Absorption Spectroscopy in Oxygen Reduction Reaction.

The construction and understanding of synergy in well-defined dual-atom active sites is an available avenue to promote multistep tandem catalytic reactions. Herein, we construct a dual-hetero-atom catalyst that comprises adjacent Cu-N4 and Se-C3 active sites for efficient oxygen reduction reaction (ORR) activity. Operando X-ray absorption spectroscopy coupled with theoretical calculations provide in-depth insights into this dual-atom synergy mechanism for ORR under realistic device operation conditions. The heteroatom Se modulator can efficiently polarize the charge distribution around symmetrical Cu-N4 moieties, and serve as synergistic site to facilitate the second oxygen reduction step simultaneously, in which the key OOH*-(Cu1 -N4 ) transforms to O*-(Se1 -C2 ) intermediate on the dual-atom sites. Therefore, this designed catalyst achieves satisfied alkaline ORR activity with a half-wave potential of 0.905 V vs. RHE and a maximum power density of 206.5 mW cm-2 in Zn-air battery.

Demystifying the Druggability of the MYC Family of Oncogenes.

The MYC family of oncogenes (MYC, MYCN, and MYCL) encodes a basic helix-loop-helix leucine zipper (bHLHLZ) transcriptional regulator that is responsible for moving the cell through the restriction point. Through the HLHZIP domain, MYC heterodimerizes with the bHLHLZ protein MAX, which enables this MYC-MAX complex to bind to E-box regulatory DNA elements thereby controlling transcription of a large group of genes and their proteins. Translationally, MYC is one of the foremost oncogenic targets, and deregulation of expression of the MYC family gene/proteins occurs in over half of all human tumors and is recognized as a hallmark of cancer initiation and maintenance. Additionally, unexpected roles for this oncoprotein have been found in cancers that nominally have a non-MYC etiology. Although MYC is rarely mutated, its gain of function in cancer results from overexpression or from amplification. Moreover, MYC is a pleiotropic transcription factor possessing broad pathogenic prominence making it a coveted cancer target. A widely held notion within the biomedical research community is that the reliable modulation of MYC represents a tremendous therapeutic opportunity given its role in directly potentiating oncogenesis. However, the MYC-MAX heterodimer interaction contains a large surface area with a lack of well-defined binding sites creating the perception that targeting of MYC-MAX is forbidding. Here, we discuss the biochemistry behind MYC and MYC-MAX as it relates to cancer progression associated with these transcription factors. We also discuss the notion that MYC should no longer be regarded as undruggable, providing examples that a therapeutic window is achievable despite global MYC inhibition.