Aims: This study was conducted to determine the attitudes and behaviors of intensive care nurses towards end-of-life care and their psychological well-being levels. Methods: This descriptive study was conducted using a face-to-face interview technique with 101 nurses working at Aksaray University Training and Research Hospital between March and July 2023. Data were collected using the Nurse Introductory Information Form, Intensive Care Nurses’ Attitudes and Behaviors Towards End-of-Life Care Scale, and Psychological WellBeing Scale. The relationship between the variables was investigated with the Spearman correlation test. Results: It was determined that 74.3% of the nurses were women, 60.4% were single, and 61.4% had worked in the profession for 1-5 years. It was determined that nurses’ attitudes and behaviors towards end-of-life care scale scores showed a significant difference depending on whether they received information about end-of-life care and that there was a significant difference between the psychological well-being scale score and satisfaction with the department they worked in (p<0.05). There is a moderate and positive relationship between the behavioral scale and the psychological well-being scale mean scores (r=0.368, p<0.001). Conclusion: The study found that as nurses’ attitudes and behavior levels towards end-of-life care increased their psychological well-being levels also increased. It is recommended that nurses evaluate their psychological well-being and quality of life within the scope of holistic care and that studies be carried out to provide training and consulting services to nurses in this direction.