Horse Welfare Research Articles

A synopsis of wearable commercially available biometric‐monitoring devices and their potential applications during gallop racing

SummaryAmidst discourse surrounding the racing industry's social licence to operate, the welfare of racehorses and jockeys is often at the forefront of discussions. Specifically, catastrophic events and the sudden death of horses on race days tend to capture media attention and often place the racing industry under public scrutiny. While such occurrences are not frequent, they have negative ramifications on the welfare of both racehorses and jockeys. The development and application of advanced monitoring technology could provide greater insight into the physiological precursors that immediately precede sudden death on race days and enable retrospective analyses of catastrophic events that take place. Most importantly, the application of such technology could ultimately aid racetrack personnel in identifying and intervening with horses at increased risk of catastrophic events, right up until the start of a race. This would inevitably improve the safety and welfare of horses and jockeys. Thus, the current review details key cardiovascular, respiratory and thermometry biometrics for racehorses and examines the currently available commercial devices with potential applications during racing. This review also highlights the importance of gathering biometric data for retrospective analyses of catastrophic events and their implications on the welfare of racehorses and jockeys.

Evaluation of thermoregulation of horses (Equus caballus) submitted to two methods of post-exercise cooling, in hot and humid climate conditions, in the Eastern Amazon.

In Brazil, the study on equine thermoregulation has not shown considerable advances, moreover, in the Amazon, this is a little explored aspect. This study aims to evaluate the thermoregulation of horses submitted to two methods of post-exercise cooling, in the climatic conditions of the Eastern Amazon. The experiment was conducted at Centro Hipico, Ananindeua, Para, for 15 days. Ten male horses, castrated of the Brazilian breed, aged 13 years and with an average weight of 482.3 kg were used. Equestrianism, which was exercised with pre-established protocol in the arena and track, for 30 min. After the exercise, the animals were divided into two groups for application of the treatments, which were two cooling methods, such as a bath with water at room temperature (about 25°C) and a hiper cooling method (6-9°C). During the experimental period, air temperature (AT) and relative humidity (RH) data were recorded and the Temperature and Humidity Index (THI) was calculated. Data from the rectal temperature (RT), heart rate (HR), respiratory rate (RR) and Body surface temperature (BST) of the animals were also measured at three moments: before exercise, after exercise and after applying the cooling methods. The adaptability index used was the Benezra Thermal Comfort Index (BTCI). The BST was performed with the help of infrared thermograph, which were the left side of the neck, thorax, rump, and right side to the armpi, before the exercise, after the exercise and after applying the cooling methods. The statistical design was completely randomized. Analysis of variance was performed using the GLM Procedure of SAS 9.1.3. After the application of the cooling techniques in the animals, the AT and THI were higher and the highest RH values were found before the exercise (87.21%). The highest RT, RR, HR, BST, and BTCI values were observed after exercise. There was no significant dierence (P > 0.05) between cooling methods. There was a highly significant and positive correlation (P < 0.01) between all physiological variables (RT, RR, HR, and BST) and the Iberian Index with AT and THI and negative with RH (P < 0.01), thus demonstrating the influence of the environment on the thermoregulation of animals. It is concluded the evaluation of stress, welfare and thermoregulation of horses submitted to two methods of post-exercise cooling, in the climatic conditions of the Eastern Amazon, demonstrated that the studied cooling methods showed to reduce the rectal temperature, the respiratory rate, the heart rate and the body surface temperature with equal efficiency. However, in terms of practical ease of use, the room temperature water cooling method has proven to be more practical.

The Effect of Insect Bite Hypersensitivity on Movement Activity and Behaviour of the Horse.

Insect bite hypersensitivity (IBH) associated with Culicoides biting midges is a common allergic skin disease in horses, reducing the welfare of affected horses. This study investigated the effect of IBH on animal welfare and behaviour and assessed a new prophylactic insect repellent. In total, 30 horses were recruited for a prospective cross-over and case-control study. Clinical signs of IBH, inflammatory markers in skin biopsies and behavioural data (direct observations, motion index) were scored longitudinally during two consecutive summers. No differences were observed in the total number of itching behaviours or motion index between IBH-affected horses and controls, but higher numbers of itching behaviours were observed in the evening. IBH-affected horses showed both clinical and histopathological signs of inflammatory skin lesions, with even short periods of scratching being associated with moderate/severe inflammatory skin lesions. In order to improve the welfare of the IBH-affected horses, they should be stabled/given extra protection in the evening and even short-term exposure to Culicoides should be avoided. Preliminary results showed that the repellent tested can be used as a safe and non-toxic prophylactic to potentially reduce allergen exposure in horses with IBH, but further studies are needed to determine its efficacy.

Modulatory effects of ergothioneine on behavioural responses of Arabian stallions following a 2,000 m race during the rainy and hot-dry seasons in a tropical environment

Abstract Experiments were performed to determine the effects of ergothioneine (ERG) on behavioural responses of stallions to exercise during hot-dry and rainy seasons. Eighteen healthy stallions were divided into three groups of six horses each. Group I (ERG + Exercise) was treated with ERG before exercise, group II was treated after exercise (Exercise + ERG), and group III (Exercise only) was not treated but subject to exercise. The stallions were subjected to a race of 2,000 m at maximum speed. The time spent standing by the horses in the ERG + Exercise and Exercise + ERG groups (1430.31 ± 30.73 s/30 min and 1403.32 ± 32.43 s/30 min, respectively) were higher (P &lt; 0.0001) than the time spent standing in the Exercise-only group (1033.26 ± 21.19 s/30 min). The number of times the stallions bit their cribs per 30 min in the ERG + Exercise (0.38 ± 0.02/30 min) and Exercise + ERG (0.67 ± 0.0530 min) groups was lower (P &lt; 0.0001) than that recorded in the Exercise-only group (3.51 ± 1.7730 min). It was concluded that ERG modulates behavioural responses to exercise in stallions, and the agent may be beneficial in alleviating exercise-induced stress responses and improving the welfare of horses post-exercise.

The Effect of Stabling Routines on Potential Behavioural Indicators of Affective State in Horses and Their Use in Assessing Quality of Life.

Increasing interest in equine welfare has emphasised the need for objective and reliable behavioural indicators of horses' affective state. However, research has yielded mixed results regarding behaviours suited for industry use largely because they are subject to anthropomorphic interpretation. Stabling is commonly used to manage domesticated horses despite research indicating that it can negatively impact horse welfare, but its effect on their affective state is yet to be quantified. Ten adult horses (11.8 ± 4.4 years) were observed either on a day- (DS) or night-stabling (NS) schedule over two consecutive 24 h periods. NS horses were kept confined for significantly longer (13.60 ± 0.04 h) than DS horses (7.73 ± 0.07; t7 = 5.70; p = 0.0004). Eight behaviours occurred significantly more often during NS than DS: forward ears (t7 = 3.32; p = 0.001), neutral ears (t7 = 3.47; p = 0.001), stepping forward (t7 = 2.62; p = 0.001), stepping laterally (t7 = 2.39; p = 0.001), sternal recumbency (t7 = 2.64; p = 0.001), yawning (t7 = 2.69; p = 0.001), non-nutritive chewing (t7 = 2.49; p = 0.001), and closing eyes (t7 = 2.71; p = 0.001). These behaviours may be candidates for indicators that can be used to determine the affective state in horses and subsequently be used to assess equine quality of life and to optimise individual horse welfare.

Contribution of woody plants to horses’ diets in Mediterranean rangelands

In Mediterranean countries, shrubby and woody areas provide herds with cheap fodder. However, some horse farmers are reluctant to use woody rangelands, which they associate with low-quality feed. In this article, we explore some aspects of the feeding behavior of horses on Mediterranean shrubby and woody rangelands, in order to document their pastoral interest for horses. We observed the feeding choices of young horses grazing on typical peri-Mediterranean rangelands. Observations were repeated at two sites, with four horses per site, over four days with two observers. At each site, two horses were fitted with GPS (positioning system) collars to determine their presence in different areas of the paddocks. Ingested plants were classified in a coding grid. The chemical characteristics of each feed item were estimated by near-infrared spectroscopy (NIRS). A hierarchical classification of NIRS spectra defined four classes. Leaves, twigs, and flowers of several woody species were ingested (1‒18% of recorded bites), although herbaceous species were preferred (&gt; 80% of bites). Horses explored the entire paddocks but grazed preferentially in open areas. The differences observed between sites suggested a strong effect of local grazing conditions. The chemical composition of feed from shrubs and trees was close to that of herbaceous forbs but quite different from that of grasses. Three out of four classes associated woody plants and forbs. These classes had interesting nutritional characteristics (protein &gt; 12%). These results confirm the positive contribution of woody plants to horses' nutrition, in addition to the other roles of woody plants in the well-being of horses on pasture.

Untangling the Complex Relationships between Horse Welfare, Rider Safety, and Rider Satisfaction

ABSTRACT Securing the horse industry’s long-term future is becoming increasingly urgent due to community concerns for horse welfare; human behavior change may offer novel solutions to this challenging problem. Self-determination theory (SDT), a well-established theory in human behavior change, assumes humans are intrinsically motivated by three universal psychological needs: competence, autonomy, and relatedness. These align well with horse–human interaction. To explore the potential for human behavior change to improve horse welfare, this study investigated the relationship between horse welfare, rider safety, and rider satisfaction. An online survey asked equestrians about their horse-keeping and training practices, their riding accidents and injuries, and their level of satisfaction with their horse. Relative horse welfare and rider satisfaction were significantly related (r = 0.34, p < 0.001). Hyperreactive horse behavior (defined in this study as bucking, bolting, rearing, and spooking) and rider accidents and injuries were negatively related to rider satisfaction (r = −0.23, p < 0.001 and r = −0.16, p = 0.001, respectively). Further, goal achievement (r = 0.57, p < 0.001), rider control (r = 0.49, p < 0.001), and horse–human partnership (r = 0.62, p < 0.001) were all significantly related to rider satisfaction. A regression analysis testing the proposed SDT-based rider satisfaction model was significant (p < 0.001), with goal achievement, rider control, and horse–human partnership accounting for 63% of the variance of rider satisfaction scores. We found that horse welfare, rider safety, and rider satisfaction were related, with equestrian goal achievement (competence), horse control (autonomy), and horse-rider partnership (relatedness) all significantly contributing to rider satisfaction, suggesting that SDT may offer a useful framework for developing behavior change strategies. While more work is needed to develop and evaluate such strategies, a better understanding of intrinsic rider motivation represents an important first step.

Ensuring Animal Well-Being in Animal-Assisted Service Programs: Ethics Meets Practice Learning at Green Chimneys

Abstract This paper illustrates the complexity of meeting a high and constant standard of animal welfare at Green Chimneys, a residential treatment facility and therapeutic day school for children with psychiatric diagnosis ages K-12. Green Chimneys incorporates over 300 animals of various species in animal-assisted education and therapy programs. Ensuring the well-being of horses, farm animals, camels, reptiles, dogs, and wild birds in captivity demands a practical, systemic, and most of all, flexible philosophical animal welfare framework. Implementing a consistent animal care program, in a larger community of students and adults who often have divergent beliefs about the treatment of animals, is challenging. Examples of ethical struggles that impact practical, real life animal care and treatment decisions are included. The authors also describe their ongoing communication process regarding animal care with their team, with the goal of encouraging other programs to engage in similar conversations and recognize that ethically caring for and working with animals in a human-service setting is an ongoing journey.

Exploring the Social Reinforcing Value of Brushing in Equine-Assisted Services: A Comparative Study with Food Reinforcement

Abstract Brushing is one of the most common human-horse interactions in Equine-Assisted Services (EAS). Previous research has shown both positive and negative effects of brushing on horses’ behavior and physiological states. One of the primary reasons horses are included in services such as mental and behavioral healthcare is to provide human clients with positive relational experiences; however, more empirical research is needed to determine if horses also receive socially derived benefits from their interactions with human clients. Therefore, we set out to determine whether brushing can function as a social reinforcer for horses utilized in EAS in comparison to food reinforcement. Six EAS horses from a university-based Premier Accredited Professional Association of Therapeutic Horsemanship, International center in the Southwestern Region of the United States participated in a single-subject reversal ABCABC design experiment, in which stimuli (A: Brushing, B: Food, and C: Control [Extinction]) were provided contingent on an arbitrary behavior (nose touch to the experimenter’s closed fist). All horses exhibited maximal or near-maximal responding in the food condition, but low responding in the brushing and control conditions. Data indicated that brushing was not an effective reinforcer for horses compared to food, and this could suggest brushing is a low-value reinforcer. Alternatively, it is possible to access to food at relatively short intervals in time during the experiment may have impacted the reinforcing value of brushing. Human-animal interaction proponents are increasingly calling attention to the need to ensure that equine-inclusive healthcare services address horse welfare, including the promotion of positive welfare experiences. Results from this study support the need for more research on identifying optimal interactions that can promote mutual benefits to both humans and horses participating in EAS.

Longitudinal Training and Workload Assessment in Young Friesian Stallions in Relation to Fitness, Part 2-An Adapted Training Program.

Young Friesian stallions have to complete a 10-week training program (70-day test) for acceptance as a breeding stallion. Part one of this study showed that the 70-day test was too intense and led to reduced fitness. In the present (part two) study, the effects of an adapted training program were studied. Training frequency and minutes of cantering per week were lowered compared with part one. The external workload (EW) of sixteen stallions (3.4 ± 0.8 years) was monitored during the 6-weeks before testing (preparation period) as well as during the 70-day test. Standardized exercise tests (SETs) were performed in week 1 (SET-I), 6 (SET-II), and 10 (SET-III) of the 70-day test, measuring heart rate (HR) and plasma lactate concentration (LA). Linear mixed effect models were used to analyze the EW, and the HR and LA related to the SETs. The EW increased from the preparation period to the 70-day test; this increase in the EW was lower compared with the original training program. The horses showed lower HR and LA levels in SET-III compared with SET-I. The young Friesian horses were at risk to be overtrained in part one, but the adapted training program resulted in increased fitness. This study shows that a carefully chosen program is necessary to improve the performance and welfare of (young) horses.

Equine osteoarthritis: Strategies to enhance mesenchymal stromal cell-based acellular therapies.

Osteoarthritis (OA) is a degenerative disease that eventually leads to the complete degradation of articular cartilage. Articular cartilage has limited intrinsic capacity for self-repair and, to date, there is no curative treatment for OA. Humans and horses have a similar articular cartilage and OA etiology. Thus, in the context of a One Health approach, progress in the treatment of equine OA can help improve horse health and can also constitute preclinical studies for human medicine. Furthermore, equine OA affects horse welfare and leads to significant financial losses in the equine industry. In the last few years, the immunomodulatory and cartilage regenerative potentials of mesenchymal stromal cells (MSCs) have been demonstrated, but have also raised several concerns. However, most of MSC therapeutic properties are contained in their secretome, particularly in their extracellular vesicles (EVs), a promising avenue for acellular therapy. From tissue origin to in vitro culture methods, various aspects must be taken into consideration to optimize MSC secretome potential for OA treatment. Immunomodulatory and regenerative properties of MSCs can also be enhanced by recreating a pro-inflammatory environment to mimic an in vivo pathological setting, but more unusual methods also deserve to be investigated. Altogether, these strategies hold substantial potential for the development of MSC secretome-based therapies suitable for OA management. The aim of this mini review is to survey the most recent advances on MSC secretome research with regard to equine OA.

Bit use and its relevance for rider safety, rider satisfaction and horse welfare in equestrian sport

Using a bit to communicate with and control the horse while riding is one of the longest standing traditions in horse riding. However, horses’ aversion to bits and high levels of bit-induced trauma have led some to question whether this practice is commensurate with modern understanding of animal welfare. This study represents one of the first attempts to compare horses ridden with and without a bit in terms of horse welfare, rider safety and rider satisfaction. Australian recreational and sport horse riders (n = 399, 94.4% female; Mdn age=46.0 years) from a range of riding disciplines were anonymously surveyed online. Only n = 20 (5.0%) participants reported riding without a bit, however, this is likely consistent with the recreational horse-riding population, given riding with a bit is mandated in most equestrian sports. A whole sample analysis and a case-matched analysis were performed where cases were matched based on horse breed and age, discipline, and rider age. Whole sample analyses found horses ridden without a bit have better relative welfare scores for management, and during riding and handling compared to horses ridden with a bit (Mdn 13.0, IQR 2.0 and Mdn 11.0, IQR 5.0; Mdn 35.0, IQR 9.0 and Mdn 32.0, IQR 6.0; and Mdn 24.0, IQR 3.25 and Mdn 23.0 IQR 4.0 respectively, p < 0.05), but did not differ in relation to health. Fewer ridden hyperreactive behaviours (bucking, spooking, rearing and bolting) were reported for bitless horses compared with bitted horses (Mdn 0.0, IQR 1.0 and Mdn 1.0, IQR 4.0, respectively, p = 0.04), however, rider safety and perceived control were similar for both groups. Riders of bitless horses were more satisfied than riders of bitted horses (Mdn 16.0, IQR 2.0 and Mdn 14.0, IQR 4.0, respectively, p = 0.01) and reported better horse-rider partnerships (Mdn 4.0, IQR 0.0 and Mdn 3.0, IQR 1.0, respectively, p < 0.001). More riders of bitless horses had knowledge of learning theory than riders of bitted horses (55.0% and 23.0% respectively, p = 0.003). Results from the case-matched analysis were consistent with the whole sample results, except for rider satisfaction and knowledge of learning theory which were not statistically significant. These findings suggest that bitless riding is not related to reduced horse control or rider safety, but is related to greater rider satisfaction and improved horse-rider partnership. Considering current challenges to the industry’s social licence to operate due to poor horse welfare, increased adoption of bitless riding may present a valuable opportunity to improve horse welfare.

Sustainability and the Thoroughbred Breeding and Racing Industries: An Enhanced One Welfare Perspective.

As society debates the use of animals in sport, entertainment, and leisure, there is an increasing focus on the welfare, social, and ecological impacts of such activities on the animals, human participants, people close to them, and the physical environment. This article introduces the "Enhanced One Welfare Framework" to reveal significant costs and benefits associated with Thoroughbred breeding and racing globally. In addition, relative to calls to ban horseracing and similar activities as part of sustainability approaches that focus chiefly on animals, the "Enhanced One Welfare Framework" is better positioned politically to guide discussions that renegotiate the conditions under which horses are used for sport and the impact racing has on humans and the planet. In 2020, the International Federation of Horseracing Authorities issued its minimum horse welfare standards based on the Five Domains model, positioning lifelong horse welfare as "fundamentally important to the viability and sustainability of the industry". In this article, we critique the One Welfare framework's historic lack of focus on sport and enhance it by including sport, leisure, and entertainment and framing it within the Five Domains model. We offer a novel extension of the Five Domains model beyond animal welfare to consider human welfare and the physical environmental impacts of the sport, leisure, and entertainment industries and propose innovations that may help thoroughbred breeding and racing assure a sustainable future.

Does a Working Knowledge of Learning Theory Relate to Improved Horse Welfare and Rider Safety?

ABSTRACT Training and riding directly effects horse welfare, highlighting the potential for training methods to improve both horse welfare and human safety. Learning theory is considered the most appropriate scientific foundation for horse training methods, yet equestrians’ knowledge of learning theory is reportedly low. The relationship between equestrians’ knowledge of learning theory terminology (LT) and horse welfare and rider safety was investigated to determine if rider knowledge of LT contributes to improved horse welfare and human safety. A sample of 394 Australian recreational and sport horse riders completed an online survey. Ridden horse welfare was assessed using validated husbandry and behavioral indicators. Rider safety was assessed by asking participants about ridden accidents and injuries. Rider knowledge of LT was assessed by asking participants to correctly identify scenarios that depicted three key operant conditioning terms: negative and positive reinforcement, and punishment. Only 24.6% of the sample correctly identified all three terms (the criteria for having a basic knowledge of LT), suggesting knowledge of LT has increased among equestrians but remains low. However, rider knowledge of LT was not significantly related to improved horse welfare or rider safety. It is uncertain why knowledge of LT remains so low among equestrians and why rider knowledge of LT did not translate to welfare and safety benefits as predicted. In facilitating the development of workable solutions to address the dual issues of poor horse welfare and human safety, we explore several possibilities, including a proposed new horse training framework that may enable learning theory to be leveraged more fully and deliver expected benefits.

Cortisol Variations to Estimate the Physiological Stress Response in Horses at a Traditional Equestrian Event.

In many countries, horses remain involved in traditional equestrian events such as those celebrated in Menorca (Balearic Islands, Spain) every year since at least the 14th century. The present study aimed to evaluate the variations in salivary cortisol concentrations to estimate the physiological stress response in horses at the Menorca patronal festivals. Two different editions (years 2016 and 2018) of the festivals in honor of the Virgin of Grace in Maó (Menorca, Spain) were studied. Nineteen and seventeen Pure Breed Menorca stallions were included in the study, respectively. The stallions were aged between seven and twelve years. During celebrations, samples were collected before the start of the festivals between 8-9 a.m. and during the festivals at 8-9 p.m. On the second day of celebrations, the samples were collected at 8-9 a.m. and 3-4 p.m. Finally, on the day after the festivals, one sample was collected at 8-9 p.m. Additionally, a control group was sampled at 8-9 a.m., 3-4 p.m., and 8-9 p.m. Salivary cortisol concentrations were assessed by using a commercial enzyme immunoassay kit specially validated to quantify salivary cortisol in horses. Salivary cortisol concentrations did not show significant differences between sampling hours in the control group (p > 0.05). All the samples collected during festivals were significantly higher than samples of the control group (p < 0.05). Within the twenty-four hours after the end of the celebrations, cortisol concentrations returned to baseline levels and did not differ significantly from the control group (p > 0.05). Hence, the present study describes that the participation of the horses in these particular acts generate an acute and transitory stress response. Overall, the current work provides a reasonable basis for future research on the stress physiology and well-being of horses participating in traditional celebrations or similar events.

Working horses welfare assessment and their owners perceive in middle and north Darfur states Sudan

Working horses in Low and Middle income countries have a significant impact on people's lives. In spite of their best, the well-being of Sudanese working horses are neglected. There is no information on Sudanese working horse’s welfare. The purpose of this study was to investigate working horse welfare and owners' perceptions in two states. A methodology using owner interviews and direct parameters as well as body condition score and gait abnormality was applied. A total of 150 working horses and their owners were assessed (Middle Darfur =100, North Darfur =50). The results revealed that there was a significant difference between the body condition score of horses from the north and middle state, with 34% and 18% being thin, respectively. There was a significant difference (P = 0.040) in nasal discharge, with 24% and 18% of the horses from the north and middle, respectively. Moreover, there was a significant difference (P < 0.001) in dirty coat conditions, appeared in 62% and 33% of the horses from the north and middle, respectively. Horses that had external parasites differed significantly between both regions (P < 0.0001), with 70% and 23% having parasites in the north and middle, respectively. No significant differences were found between the horses from the north and middle state in terms of skin wounds and kicking behavior. We conclude that the northern state suffers from more direct welfare issues than the middle state. However, indirect parameter measures were worse in the middle state than in north. Owner awareness and additional research are required for both states.

Horse Housing on Prince Edward Island, Canada: Attitudes and Experiences Related to Keeping Horses Outdoors and in Groups.

Limited research has assessed the "human dimension" of horse care. The aims of this study were to (1) understand horse owner attitudes toward horse welfare when kept outdoors versus indoors and in groups versus individually, (2) compare horse owner attitudes toward horse welfare with the ways in which they house their horses, and (3) explore horse owner reasons for and challenges with their horses' housing. Seventy-six horse owners in Prince Edward Island, Canada completed a questionnaire. Non-parametric tests and quantitative content analysis were used for data analysis. Consistent with the way horses were kept, most (82-96%) owners agreed that horses' physical health, mental well-being, and natural living were better when kept outdoors and in groups. Fewer (64-68%) participants agreed that the horses' standard of care was better when kept outdoors or in groups. Results show associations between owners whose attitudes suggest indoor and/or individual housing is better for horse welfare and keeping their horses indoors part-time and/or individually. Two overarching themes were developed from owners' responses regarding their reasons and challenges related to the ways in which horses were housed: horse-centered and owner-centered care. The results indicate that horse owners' choices about their horses' housing correspond to beliefs about improved horse welfare.

Maximising collaboration for equine health: working with livery yard owners and managers

Livery yard owners and managers are important stakeholders in managing the health and wellbeing of horses within their yards, yet their importance is often overlooked. Finding ways to collaborate positively with local livery yards could help veterinary professionals on many levels, from offering optimised preventative health strategies to the horses within local yards, through to ensuring trust and good professional relationships with managers and their clients.

How equestrians conceptualise horse welfare: Does it facilitate or hinder change?

More than ever the welfare of horses in equestrian sport is in the spotlight. In response to this scrutiny, one peak body, the Federation Equestre Internationale (FEI) has created an Equine Ethics and Wellbeing Commission to protect their sport's longevity. However, for welfare-based strategies to be successful, the conceptualisation of horse welfare must align across various stakeholders, including the general public. The value-laden nature of welfare makes agreement on its definition, even among scientists, difficult. Given little is known about how equestrians conceptualise horse welfare, we interviewed 19 Australian amateur equestrians using a semi-structured format. Systems thinking and the Five Domains Model provided the theoretical framework and informed our methods. Using reflexive thematic analysis, three themes were identified: (1) good horse welfare is tangible; (2) owners misinterpret unwanted horse behaviour; and (3) equestrians publicly minimise horse welfare issues but are privately concerned. Our results highlight participants' conceptualisations of horse welfare do not align with the Five Domains Model; participants' ideal of prioritising horse welfare does not align with their practice; and there is inconsistency between what participants share publicly and what they think privately about horse welfare. These findings can inform the development of programmes to improve ridden horse welfare throughout the horse industry. As a starting point, programmes that provide a safe space for equestrians to explore their private horse welfare concerns, and programmes that build a partnership mindset to facilitate knowledge exchange between all stakeholders are needed.

Biomarkers of Stress and Inflammation in Assessing the Quality of Welfare of Conditioned Vaquejada Horses

Biomarkers of Stress and Inflammation in Assessing the Quality of Welfare of Conditioned Vaquejada Horses