This study explores the influence of plasma gas flow rate (PGFR) on the defects, microstructure evolution and mechanical properties during plasma arc welding of Ti6Al4V titanium alloy thin sheets using microscopic analysis, spectroscopic analysis, tensile and microhardness tests. The variation in PGFR affects the welding arc in terms of its stability, pressure and constriction which results in transformation of arc from conduction mode to keyhole mode and changes in microstructure as well. All the other welding process parameters were kept constant except for PGFR which was varied to investigate its significance. Excess weld metal was observed under the weld bead is an attribute of keyhole formation. Macrographs exhibits increments in weld geometry measurements whereas weld defects such as lack of penetration and porosity decreased with increased PGFR. The microstructural examination showed a variety of phase formations that includes majorly with acicular α and Widmanstätten α morphologies. Tensile strength and hardness at the weld region of the welded joints increases with increase in PGFR. An oxygen rich brittle subsurface layer called α-case morphology was observed at the weld region of 1 L/min joint causes significant reduction in ductility.