This article presents a bi-level non-uniform cellular automata (CA) algorithm for the solution of sizing, topology and layout optimization of truss structures. The non-uniform CA was successfully used in a previous study to solve the weight optimization problem of truss structures for topology and sizing (El Bouzouiki, Sedaghati, and Stiharu 2021. Computers & Structures 242: 106394). In this article, an extended version of the non-uniform CA algorithm is proposed, based on the fully stressed design approach and the distribution of strain energy within the structure, to find the optimal position of the cell’s (joint’s) coordinates. The proposed non-uniform CA algorithm can solve the minimum weight optimization problem of truss structures subjected to both stress and displacement constraints. Several benchmark problems are presented to demonstrate the efficiency and accuracy of the proposed methodology.