Within the domain of macronutrients, nitrogen has garnered significant recognition as a pivotal element due to its profound influence on both the vegetative growth and quality attributes of a crop. An abundance of scientific research has consistently demonstrated that attaining optimal productivity necessitates the meticulous management of soil moisture availability, complemented by the timely provision of nitrogen during critical growth stage intervals. The study aimed to optimize the nitrogen concentration in a hydroponic solution by testing a range of concentrations. The objective was to systematically examine the effects of different nitrogen levels and determine the optimal concentration for the desired outcomes. The assessment was carried out in a meticulously controlled greenhouse environment from October 2019 to April 2021, with the primary objective of systematically investigating the impact on quality characteristics specific to the rosa variety of cherry tomato. To investigate the effects of different nitrogen concentrations, four distinct levels of 4 mM, 6 mM, 8 mM, and 10 mM per litre of nutrient solution were employed. The nitrogen source consisted of a combination of NO3- and NH4+, with a ratio of 40:60, respectively. The nutrient solution with an 8 mM nitrogen concentration exhibited a significantly augmented effect on increasing the equatorial and polar diameter of fruits, average fruit weight, pericarp thickness, as well as the accumulation of total chlorophyll, ascorbic acid, lycopene, β-carotene, and total soluble solids (TSS) content. These findings demonstrate the pronounced and positive influence of the 8 mM nitrogen concentration on the aforementioned quality characteristics.