It is generally admitted that opioids can stimulate the release of both prolactin (PRL), the opiates induced hyperprolactinemia via dopamine receptor blockade. Prolactin hormone (PRL) plays an important role in conjunction with some hormones such as GH, PTH, ACTH, and T4 to stimulating body growth. The prolactin hormone has an important role in feeding behavior, as its role is evidenced by its effect on metabolism. In migratory birds, fat is stored in its body before migration, and the hormone prolactin activates the process of building and storing fats lipogenesis and Deposition of Fats. The hypothesis of this study is to investigate the role of natural stimuli such as sound in stimulating peptide hormones in the body and thus weight gain in chickens. 100 birds of 1 day old were used it was divided into two treatments, each treatment with 5 replications. The chicks heard different sounds and it was as follows: the first the control without sound CS, the second hen sound (mother sound) HS. The sound was heard from the one old day to 14 days, at a rate of 3 times a day, in the morning, in the afternoon and at night, for an hour each time. Chicks were raising to 42 day. The hormone was estimated in three stages, before exposure to sound 30 minutes during exposure directly after exposure for 30 minutes, and the prolactin hormone concentration was calculated each time, the results show: Significant increase (P<0.05) in live body weight cumulative, weight gain cumulative, relative growth rate cumulative, and total feed conversion ratio to HS compared with CS. Significant improvement (P<0.05) in the concentration of the hormone prolactin of HS in the period after exposure to sound 30 minutes during exposure directly after exposure for 30 minutes at the age of 14 days (the end of the exposure to the sound of the period). As mentioned above, the opioids stimulate prolactin, but the question here who is responsible for stimulating the opioids? Opioids secretion is linked to the body’s sense of well-being and well-being, as it is secreted naturally to aid in rest and relaxation, and this is often noticed on the organism when it feels safe and comfortable. Chicks, when it hearing her mother’s sound, gives it a powerful stimulus, and they become more active and more and less fearful and amazement. This will release the opioid, which in turn stimulates prolactin. PRL and GH are secreted from the anterior pituitary gland and affect a wide variety of physiological functions in birds. Similarly, a relationship between plasma GH concentration and body growth is well documented. There is a chemical similarity between prolactin hormone and growth hormone, so there is a similarity in biological functions, as prolactin stimulates physical growth and weight gain in birds, reptiles and mammals due to its similarity with growth hormone as the injection of prolactin causes a significant increase in body weight.