The 4th edition of the Pre-Participation Physical Evaluation (PPE) recommends functional testing for the musculoskeletal portion of the examination. However, limitations exist concerning currently recommended functional test components and the feasibility of implementing this test battery in the secondary school setting. In particular, test-retest reliability for recommended functional tests across secondary school settings has yet to be established. PURPOSE: Determine test-retest reliability of three functional tests utilized in the Functional Pre-Participation Physical Evaluation (FPPE) project. METHODS: A convenience sample of four high schools currently enrolled in the FPPE project participated. Prior to the start of their competitive sports seasons, high school athletes completed a weight-bearing lunge to assess ankle dorsiflexion range of motion (DF) as well as the single leg anterior reach (SLAR) and anterior single leg hop for distance (SLHOP) tests as part of the FPPE project. Athlete testing was conducted by the head Certified Athletic Trainer (AT) at each high school and was repeated one week after the initial test date. Intraclass correlation coefficients (ICC) using a two-way mixed effects model and an absolute agreement definition were calculated for each functional test. Separate ICCs were calculated for each AT. ICC(3,1) values were interpreted as: excellent (>0.75), fair to good (0.40-0.75), and poor (<0.40). RESULTS: 40 athletes (m/f= 23/17, 16.4±1.1 y, 1.78±0.11 m, 70.1±13.1 kg) participated in this study (10 athletes per high school). Test-retest reliability was excellent for all raters for both the DF (ICC(3,1) range: 0.817-0.975) and SLHOP tests (ICC(3,1) range: 0.832-0.963). Test-retest reliability of the SLAR was found to be excellent for two raters (ICC(3,1): 0.813, 0.876) and fair to good for two raters (ICC(3,1): 0.583,0.693). CONCLUSIONS: Test-retest reliability for functional tasks utilized in the FPPE project was generally excellent across a sample of high school ATs, supporting the utility of these tasks in longitudinal assessments in secondary school settings. Supported by NIH Grant 5R01-AR062578-02.