Hispidin is a secondary metabolite with pharmacological properties that is produced by the medicinal mushroom Phellinus linteus. In this study, we evaluated the effect of metal ion addition to grain media while determining the optimal illumination and temperature conditions for hispidin production in P. linteus 03 using solid-state fermentation. Six metal ions were added to these media and P. linteus 03 grown in these media were for hispidin production under optimal incubation temperature and illumination conditions. The highest yield and specific productivity of hispidin, and total hispidin content were achieved at 25 °C in a dark environment which was maintained throughout the entire fermentation period for brown rice and pearl barley media. Addition of Zn2+ ions to brown rice medium resulted in the highest yield and specific productivity of hispidin and total hispidin content at 6 weeks of fermentation; these were 2.53-, 2.62-, and 2.43-fold, respectively higher than those observed in the control. This study shows that hispidin production by P. linteus 03 can be enhanced by the addition of Zn2+ ions to the grain media.