Background: Job satisfaction is a quality assessment standard measure used in evaluation of employers output and outcomes. Increase in job satisfaction correlates positively with employees output. This study assessed the predictors of intern pharmacists job satisfaction ina tertiary hospital in southeast Nigeria. Methods: The study was a questionnaire web and paper-based prospective cross-sectional survey among eligible intern pharmacists who gave their informed consent to participate in the study. Data was summarized with descriptive statistics. Linear regression analysis were used to predict factors affecting intern pharmacist’s job satisfaction while correlation coefficient was used to determine the strength of association. Reliability estimation was done using the Cronbach alpha. All tests for differences between subgroup distributions were regarded as significant if p-values equal to or less than (0.05) (two-tailed test) was reported. Results: Overall, (168) intern pharmacists participated in the study out of which (87) (51.8%) were females. Majority of the respondents were resident outside the hospital premises (136) (86%) while (21) (12.5%) were also locum practitioners. Most of the interns have spent (5-9) months out of the mandatory (12) months (139) (82.7%). Accomodation within the hospital and choice of hospital educational funding had a p-value of (0.864) in the linear regression analysis while the choice of hospital was 0.003. Conclusion: Interns accommodation within the hospital and choice of hospital for inernship training were the major predictors of job satisfaction among the respondents. Job satisfaction is an essential determinant of work output and outcomes.