Abstract Introduction Personalised Medicine (PM) aims to tailor diagnostic and therapeutic pathways for patients. Despite technological advancements in this field, public awareness of PM remains limited, representing a barrier to its clinical integration. Methods We conducted a survey across eight European Countries (France, Germany, Hungary, Italy, Netherlands, Poland, Romania, Spain) via the private platform YouGov, in April 2023. Our web questionnaire explored knowledge of PM, willingness to embrace it, and trust in information delivered by mass media. Results Of 6,581 respondents, approximately 42% lacked sufficient knowledge of PM and genetic testing, while 36.3% found information about the topic not easily accessible. Healthcare providers were the most trusted source of information (according to 80% of responders), followed by educational institutions (71.4%). Education, age, and gender significantly influenced the acceptance of PM and mass media trust, highlighting the association between high education level, readiness to embrace PM, and high trust in academic sources of information. Additionally, age, gender, and geographical location influenced the perception of PM and genetic tests in various ways across Europe. Despite the geographical differences, 63.2% of respondents want to know more about PM, and 60% support its implementation in clinical practice. Conclusions Although the growing interest in PM genetic, and -omics technologies, large sections of the population still lack access to information and understanding, indicating a disconnection between technological advancements and public awareness. The study emphasizes the importance of providing focused citizen engagement initiatives to implement PM approaches. Citizen participation in defining personalised diagnostic and therapeutic pathways is vital to empower individuals and enable them to make informed health choices. Key messages • Understanding European citizens’ attitudes and needs regarding Personalised Medicine is crucial for creating policies and facilitating access to new technologies and treatments. • Despite limited knowledge, European Citizen recognize the importance of Personalised Medicine for their health, seek further understanding, and place trust in Academic Sources of Information.