Karen A. , P. Kovacs, J .F. Beckers , O. Szenci : Pregnancy Diagnosis in Sheep: Review of the Most Practical Methods. Acta Vet. Brno 2001, 70: 115-126. Various practical methods have been used for pregnancy diagnosis in sheep. Both pregnancy and fetal numbers are accurately diagnosed by using radiography after day 70 of gestation. Rectal abdominal technique detects pregnancy with an accuracy of 66 to 100% from d 49 to 109 of gestation, however, it has a low (17 to 57%) accuracy for determining multiple fetuses. Progesterone assays have a high sensitivity (88% to 100%) and a low specificity (60% to 72%) at d 16 to 18. Estrone sulphate assay accurately detects pregnant ewe at d 30 to 35. Ovine pregnancy specific protein B (PSPB) assay accurately (100%) detects pregnancy from d 26 after breeding onwards. The accuracy of progesterone, estrone sulphate and oPSPB assays for determining fetal numbers is relatively low. A-mode and Doppler ultrasonic techniques accurately detect pregnancy during the second half of gestation. Fetal numbers cannot be determined by A-mode ultrasound, while the Doppler technique needs experience to achieve high accuracy. Transrectal B-mode, real time ultrasonography identifies the embryonic vesicles as early as d 12.8 after mating, but the sensitivity of the technique for pregnancy is very low (12%) earlier than 25 d after mating. Transabdominal B-mode ultrasonography achieved high accuracy for pregnancy diagnosis (94% to 100%) and the determination of fetal numbers (92% to 99%) on d 29 to 106 of gestation. Realtime, B-mode ultrasonography appears to be the most practical and accurate method for diagnosing pregnancy and determining fetal numbers in sheep. Pregnancy diagnosis, ewe, radiography, rectal-abdominal palpation, hormonal assays, pregnancy proteins, ultrasonography Early detection of pregnancy is of considerable economic value to sheep industry. Nonpregnant ewes could be sold, reducing feed expenses, while non-pregnant lambs could be marketed at higher price than they would bring as mature ewes (Gearhart et al. 1988). Separation of the sheep herd into pregnant and non-pregnant ewes might reduce reproductive and production losses in form of abortions, stillbirths and production of weak lambs (Wani et al. 1998). Predictions of the number of fetuses would allow appropriate nutritional management of the ewes in late gestation that will prevent pregnancy toxemia (Ford 1983), minimize prelambing feeding costs, optimize birth weight, weaning weight and survivability of lambs and reduce the incidence of dystocia (Gearhart et al. 1988). In addition, the accurate information on the stage of gestation would be useful to dry off lactating females at adequate period and to monitor the females near term (Doize et al. 1997). Methods of pregnancy diagnosis Various methods have been used to diagnose pregnancy in sheep. These methods can be classified as less practical such as the management method (non-return to estrus), abdominal ACTA VET. BRNO 2001, 70: 115–126 Address for correspondence: Aly Karen Department of Obstetrics and Reproduction, Faculty of Veterinary Science, Szent Istvan University, Istvan ut., H-1400 Budapest, P. O. Box 2, Hungary Phone: +361-4784206 Fax : +361-478-4207 e-mail: akaren@univet.hu
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