Signal detection has a wide range of practical applications. Compared with traditional weak signal detection, the nonlinear effect of noise in the bistable system is typical and easy to extend, so bistable stochastic resonance detection technique has a wider applicability. In this paper, the multi-frequency weak signals detection based on under-sampling bistable stochastic resonance is studied. Based on the theory of bistable stochastic resonance, a simulation model is established to simultaneously detect multiple low-frequency weak signals. The spectrum of input and output signals is obtained through numerical simulation calculation, and the system characteristics are analyzed. When the large parameter signal does not meet the adiabatic approximation theory, the appropriate sampling coefficient can be selected to directly under-sample the input signal to make it conform to the conditions, and then the scale inverse transformation can be carried out. Finally, combined with the simulation analysis, the correctness of the under-sampling bistable stochastic resonance system to detect the multi-frequency weak signals is verified, the sampling rate is reduced, and the complexity is effectively decreased.