Weak Modulation Research Articles

Development by Genetic Immunization of Monovalent Antibodies Against Human Vasoactive Intestinal Peptide Receptor 1 (VPAC1), New Innovative, and Versatile Tools to Study VPAC1 Receptor Function.

Multi-membrane spanning proteins, such as G protein-coupled receptors (GPCRs) and ion channels, are extremely difficult to purify as native proteins. Consequently, the generation of antibodies that recognize the native conformation can be challenging. By combining genetic immunization, phage display, and biopanning, we identified a panel of monovalent antibodies (nanobodies) targeting the vasoactive intestinal peptide receptor 1 (VPAC1) receptor. The nine unique nanobodies that were classified into four different families based on their CDR3 amino acid sequence and length, were highly specific for the human receptor and bind VPAC1 with moderate affinity. They all recognize a similar epitope localized in the extracellular N-terminal domain of the receptor and distinct from the orthosteric binding site. In agreement with binding studies, which showed that the nanobodies did not interfere with VIP binding, all nanobodies were devoid of any functional properties. However, we observed that the binding of two nanobodies was slightly increased in the presence of VPAC1 agonists [vasoactive intestinal polypeptide (VIP) and pituitary adenylate cyclase-activating polypeptide-27 (PACAP-27)], but decreased in the presence of VPAC1 antagonist. As no evidence of allosteric activity was seen in VIP binding studies nor in functional assays, it is, therefore, possible that the two nanobodies may behave as very weak allosteric modulators of VPAC1, detectable only in some sensitive settings, but not in others. We demonstrated that the fluorescently labeled nanobodies detect VPAC1 on the surface of human leukocytes as efficiently as a reference mouse monoclonal antibody. We also developed a protocol allowing efficient detection of VPAC1 by immunohistochemistry in paraffin-embedded human gastrointestinal tissue sections. Thus, these nanobodies constitute new original tools to further investigate the role of VPAC1 in physiological and pathological conditions.

Synchronization of a self-sustained cold-atom oscillator

Nonlinear oscillations and synchronisation phenomena are ubiquitous in nature. We study the synchronization of self oscillating magneto-optically trapped cold atoms to a weak external driving. The oscillations arise from a dynamical instability due the competition between the screened magneto-optical trapping force and the inter-atomic repulsion due to multiple scattering of light. A weak modulation of the trapping force allows the oscillations of the cloud to synchronize to the driving. The synchronization frequency range increases with the forcing amplitude. The corresponding Arnold tongue is experimentally measured and compared to theoretical predictions. Phase-locking between the oscillator and drive is also observed.

Fibre multi-wave mixing combs reveal the broken symmetry of Fermi–Pasta–Ulam recurrence

In optical fibres, weak modulations can grow at the expense of a strong pump to form a triangular comb of sideband pairs, until the process is reversed. Repeated cycles of such conversion and back-conversion constitute a manifestation of the universal nonlinear phenomenon known as Fermi–Pasta–Ulam recurrence. However, it remains a major challenge to observe the coexistence of different types of recurrences owing to the spontaneous symmetry-breaking nature of such a phenomenon. Here, we implement a novel non-destructive technique that allows the evolution in amplitude and phase of frequency modes to be reconstructed via post-processing of the fibre backscattered light. We clearly observe how control of the input modulation seed results in different recursive behaviours emerging from the phase-space structure dictated by the spontaneously broken symmetry. The proposed technique is an important tool to characterize other mixing processes and new regimes of rogue-wave formation and wave turbulence in fibre optics.

Theory of coherent quantum phase slips in Josephson junction chains with periodic spatial modulations

We study coherent quantum phase-slips which lift the ground state degeneracy in a Josephson junction ring, pierced by a magnetic flux of the magnitude equal to half of a flux quantum. The quantum phase-slip amplitude is sensitive to the normal mode structure of superconducting phase oscillations in the ring (Mooij-Sch\"on modes). These, in turn, are affected by spatial inhomogeneities in the ring. We analyze the case of weak periodic modulations of the system parameters and calculate the corresponding modification of the quantum phase-slip amplitude.

Plasma flow around and charge distribution of a dust cluster in a rf discharge

We employ a particle-in-cell Monte Carlo collision/particle-particle particle-mesh simulation to study the plasma flow around and the charge distribution of a three-dimensional dust cluster in the sheath of a low-pressure rf argon discharge. The geometry of the cluster and its position in the sheath are fixed to the experimental values, prohibiting a mechanical response of the cluster. Electrically, however, the cluster and the plasma environment, mimicking also the experimental situation, are coupled self-consistently. We find a broad distribution of the charges collected by the grains. The ion flux shows on the scale of the Debye length strong focusing and shadowing inside and outside the cluster due to the attraction of the ions to the negatively charged grains, whereas the electron flux is characterized on this scale only by a weak spatial modulation of its magnitude depending on the rf phase. On the scale of the individual dust potentials, however, the electron flux deviates in the vicinity of the cluster strongly from the laminar flow associated with the plasma sheath. It develops convection patterns to compensate for the depletion of electrons inside the dust cluster.

Anabolic Therapy for the Treatment of Osteoporosis in Childhood.

Numerous forms of osteoporosis in childhood are characterized by low bone turnover (for example, osteoporosis due to neuromuscular disorders and glucocorticoid exposure). Anti-resorptive therapy, traditionally used to treat osteoporosis in the young, is associated with further reductions in bone turnover, raising concerns about the long-term safety and efficacy of such therapy. These observations have led to increasing interest in the role of anabolic therapy to treat pediatric osteoporosis. While growth hormone and androgens appears to be relatively weak anabolic modulators of bone mass, emerging therapies targeting bone formation pathways (anti-transforming growth factor beta antibody and anti-sclerostin antibody) hold considerable promise. Teriparatide remains an attractive option that merits formal study for patients post-epiphyseal fusion, although it must be considered that adult studies have shown its effect is blunted when administered following bisphosphonate therapy. Mechanical stimulation of bone through whole body vibration therapy appears to be much less effective than bisphosphonate therapy for treating osteoporosis in children. New anabolic therapies which target important pathways in skeletal metabolism merit further study in children, including their effects on fracture risk reduction and after treatment discontinuation.

Periodic, complexiton solutions and stability for a (2+1)-dimensional variable-coefficient Gross-Pitaevskii equation in the Bose-Einstein condensation

This paper presents an investigation of a (2 + 1)-dimensional variable-coefficient Gross-Pitaevskii equation in the Bose-Einstein condensation. Periodic and complexiton solutions are obtained. Solitons solutions are also gotten through the periodic solutions. Numerical solutions via the split step method are stable. Effects of the weak and strong modulation instability on the solitons are shown: the weak modulation instability permits an observable soliton, and the strong one overwhelms its development.

Mining the Synergistic Core Allosteric Modules Variation and Sequencing Pharmacological Module Drivers in a Preclinical Model of Ischemia.

Identifying the variation of core modules and hubs seems to be critical for characterizing variable pharmacological mechanisms based on topological alteration of disease networks. We first identified a total of eight core modules by using an approach of multiple modular characteristic fusing (MMCF) from different targeted networks in ischemic mice. Interestingly, the value of module disturbance intensity (MDI) increased in drug combination group. Second, we redefined a weak allosteric module and a strong allosteric module. Then, we identified 15 pharmacological module drivers (PMDs) by leave‐one‐out screening with a cutoff of two folds, which were at least, in part, validated by expression and variation of topological contribution. Finally, we revealed the fusional and emergent variation of PMD in core modules contributing to multidimensional synergistic mechanism in ischemic mice and rats. Our findings provide a new set of drivers that might promote the pharmacological modular flexibility and offer a potential avenue for disease treatment.

Competition between dynamic and structural disorder in a doped triangular antiferromagnet RbFe(MoO4)2

Magnetisation measurements and electron spin resonance (ESR) spectra of a doped quasi two dimensional (2D) antiferromagnet on a triangular lattice Rb1−xKxFe(MoO4)2 reveal a crucial change of the ground state spin configuration and a disappearance of a characteristic 1/3-magnetisation plateau at x = 0.15. According to theory for triangular antiferromagnets with a weak random modulation of the exchange bonds, this is a result of the competition between the structural and dynamic disorders. The dynamic zero-point or thermal fluctuations are known to lift the degeneracy of the mean field ground state of a triangular antiferromagnet and cause the spin configuration to be the most collinear, while the static disorder provides another selection of the ground state, with the least collinear structure. Low-level doping (x ≤ 0.15) was found to decrease the Néel temperature and saturation field by only few percent, while the magnetisation plateau disappears completely and the spin configuration is drastically changed. ESR spectra confirm an impurity-induced change of the so-called Y-type structure to an inverted Y-structure for x = 0.15. For x = 0.075 the intermediate regime with the decrease of width and weakening of flattening of 1/3-plateau was found.

Vocalizations, tadpole, and natural history of Crossodactylus werneri Pimenta, Cruz Caramaschi, 2014 (Anura: Hylodidae), with comments on distribution and intraspecific variation.

Crossodactylus werneri was described based on specimens collected in the 1970's at Parque Nacional do Itatiaia, being also reported for nearby localities. We collected specimens that we assigned to C. werneri, and recorded calls of the species during fieldworks at Serra das Cabras (Campinas, state of São Paulo). In this paper, we describe for the first time the vocalizations, tadpole, coloration in life, and comment on aspects of the natural history of C. werneri. Besides, the examination of specimens in zoological collections allowed us to extend the geographic range for this species. We also make remarks on morphological/chromatic variation and provide 16S rDNA sequences for the species. Adults were found along a slow-flowing streamlet with sandy/muddy bottom within a small fragment of secondary forest. Males called between sunset and first hours of the night. Advertisement call consisted of series of pulsed notes. Call duration lasted around 3 s, emitted at the highest rate of 17 calls per minute and six notes per second. Note duration lasted around 18 ms. Notes had poorly defined pulses (irregular and/or weak amplitude modulations along the note). The dominant frequency was about 3380 Hz. Territorial call had a long, well-defined pulsed portion followed by a higher-amplitude "squeak". The dominant frequency was around 3400 Hz. Tadpoles were essentially similar to those of other Crossodactylus species, except by not having nostril ornamentation. Our record of C. werneri in Serra das Cabras might be regarded a rediscovery of this species since C. werneri had not been recorded for more than 30 years until our first record of C. werneri in the field from 2011 and subsequent years. Our record is approximately 100 km west, and Mococa 200 km northwest, from Santo Antônio do Pinhal, the westernmost previous record for C. werneri up to date. Gene sequences (16S rRNA) give insights into the genetic divergence between C. werneri and some congeners.

Fusion that matters: convolutional fusion networks for visual recognition

In recent years, deep learning has been successfully applied to diverse multimedia research areas, with the aim of learning powerful and informative representations for a variety of visual recognition tasks. In this work, we propose convolutional fusion networks (CFN) to integrate multi-level deep features and fuse a richer visual representation. Despite recent advances in deep fusion networks, they still have limitations due to expensive parameters and weak fusion modules. Instead, CFN uses 1 × 1 convolutional layers and global average pooling to generate side branches with few parameters, and employs a locally-connected fusion module, which can learn adaptive weights for different side branches and form a better fused feature. Specifically, we introduce three key components of the proposed CFN, and discuss its differences from other deep models. Moreover, we propose fully convolutional fusion networks (FCFN) that are an extension of CFN for pixel-level classification applied to several tasks, such as semantic segmentation and edge detection. Our experiments demonstrate that CFN (and FCFN) can achieve promising performance by consistent improvements for both image-level and pixel-level classification tasks, compared to a plain CNN. We release our codes on https://github.com/yuLiu24/CFN. Also, we make a live demo (goliath.liacs.nl) using a CFN model trained on the ImageNet dataset.

Non-reciprocal optical mirrors based on spatio-temporal acousto-optic modulation

Here, we investigate a scheme to realize free-space isolators and highly non-reciprocal mirrors with weak modulation imparted by an acoustic wave. We propose a strategy to dramatically break time-reversal symmetry by exploiting resonant interactions between a travelling acoustic wave and highly resonant Fabry–Pérot modes, inducing total reflection of an optical beam at a given angle, and no reflection at the negative angle. Different from conventional acousto-optic isolators, which are based on non-resonant frequency conversion and filtering, our proposal operates at the frequency of the optical signal by tailoring the resonant properties of the structure as well as the acoustic wave frequency and intensity, enabling 50 dB isolation with modest modulation requirements. Operation in the reflection mode allows for close-to-zero insertion loss, enabling disruptive opportunities in our ability to control and manipulate photons.

Distributed Measurement of Fermi–Pasta–Ulam Recurrence in Optical Fibers

We experimentally demonstrate the distributed measurement of Fermi–Pasta–Ulam (FPU) recurrence in an optical fiber. Square-shaped pulses with weak modulation are injected into a single-mode fiber to exhibit FPU recurrence. Rayleigh backscattering signals of various sidebands are recorded to measure the power vibration along the optical fiber. The validity of the distributed method is confirmed both by the numerical simulation and the cutting-back method. Compared with the traditional cutting-back method, the distributed method has significant advantages, such as short measurement time, low cost, and good repeatability. We also believe that the distributed method has a great potential for the measurement of other nonlinear effects in optical fiber.

A HOMOLOGICAL CHARACTERIZATION OF KRULL DOMAINS

In this article, we introduce a new type of projective modules, called the weak w-projective module. By using this type of modules, we give a homological characterization of Krull domains. More pre...

Quantum noise spectra for periodically driven cavity optomechanics

A growing number of experimental set-ups in cavity optomechanics exploit periodically driven fields. However, such set-ups are not amenable to analysis using simple, yet powerful, closed-form expressions of linearized optomechanics, which have provided so much of our present understanding of experimental optomechanics. In the present paper, we formulate a new method to calculate quantum noise spectra in modulated optomechanical systems, which we analyze, compare, and discuss with two other recently proposed solutions: we term these (i) frequency-shifted operators (ii) Floquet and (iii) iterative analysis. We prove that (i) and (ii) yield equivalent noise spectra, and find that (iii) is an analytical approximation to (i) for weak modulations. We calculate the noise spectra of a doubly-modulated system describing experiments of levitated particles in hybrid electro-optical traps. We show excellent agreement with Langevin stochastic simulations in the thermal regime and predict squeezing in the quantum regime. Finally, we reveal how experimentally inaccessible spectral components of a modulated system can be measured in heterodyne detection through an appropriate choice of modulation frequencies.

Signature of tilted Dirac cones in Weiss oscillations of8−Pmmnborophene

Polymorph of $8\ensuremath{-}Pmmn$ borophene exhibits anisotropic tilted Dirac cones. In this work, we explore the consequences of the tilted Dirac cones in magnetotransport properties of a periodically modulated borophene. We evaluate modulation-induced diffusive conductivity by using linear response theory in low temperature regime. The application of weak spatial modulation (electric, magnetic or both) gives rise to the magnetic-field-dependent nonzero oscillatory drift velocity which causes Weiss oscillation in the longitudinal conductivity at low magnetic field. The Weiss oscillation is studied in the presence of a weak spatial electric, magnetic, and both modulations individually. The tilting of the Dirac cones gives rise to an additional contribution to the Weiss oscillation in longitudinal conductivity. Moreover, it also enhances the frequency of the Weiss oscillation and modifies its amplitude too. Most remarkably, it is found that the presence of both out-of-phase electric and magnetic modulations can cause a sizable valley polarization in diffusive conductivity. The origin of valley polarization lies in the opposite tilting of the two Dirac cones at two valleys.

A class of states supporting diffusive spin dynamics in the isotropic Heisenberg model

The spin transport in the isotropic Heisenberg model in the sector with zero magnetization is generically super-diffusive. Despite that, we here demonstrate that for a specific set of domain-wall-like initial product states it can instead be diffusive. We theoretically explain the time evolution of such states by showing that in the limiting regime of weak spatial modulation they are approximately product states for very long times, and demonstrate that even in the case of larger spatial modulation the bipartite entanglement entropy grows only logarithmically in time. In the limiting regime we derive a simple closed equation governing the dynamics, which in the continuum limit and for the initial step magnetization profile results in a solution expressed in terms of Fresnel integrals.

Asymmetric W-shaped and M-shaped soliton pulse generated from a weak modulation in an exponential dispersion decreasing fiber**Project supported by the National Natural Science Foundation of China (Grant No. 11475135), the Fund from Shaanxi Province Science Association of Colleges and Universities (Grant No. 20160216), and Guangxi Provincial Education Department Research Project, China (Grant No. 2017KY0776).

We study localized waves on continuous wave background in an exponential dispersion decreasing fiber with two orthogonal polarization states. We demonstrate that asymmetric W-shaped and M-shaped soliton pulse can be generated from a weak modulation on continuous wave background. The numerical simulation results indicate that the generated asymmetric soliton pulses are robust against small noise or perturbation. In particular, the asymmetric degree of the asymmetric soliton pulse can be effectively controlled by changing the relative frequency of the two components. This character can be used to generate other nonlinear localized waves, such as dark–antidark and antidark–dark soliton pulse pair, symmetric W-shaped and M-shaped soliton pulse. Furthermore, we find that the asymmetric soliton pulse possesses an asymmetric discontinuous spectrum.

Experimental evidence for weaker endogenous inhibition of trigeminal pain than extra-trigeminal pain in healthy individuals.

Background and objectives The prevalence of pain syndromes that affect the territories innervated by the trigeminal nerve, such as headaches, is one of the highest and ranks second only to low back pain. A potential mechanism underlying this high prevalence may be a relatively weak endogenous pain modulation of trigeminal pain. Here, we sought to systematically compare endogenous pain modulation capabilities in the trigeminal region to those of extra-trigeminal regions in healthy subjects. Methods Healthy, pain free subjects (n = 17) underwent a battery of quantitative sensory testing to assess endogenous pain inhibition and pain enhancement efficiencies within and outside the trigeminal innervated region. Measurements included conditioned pain modulation (CPM), temporal summation of pain (TSP) and spatial summation of pain (SSP). Results Testing configurations that included trigeminal-innervated body regions displayed significantly weaker CPM when compared to extra-trigeminal innervated areas. SSP magnitude was smaller in the ophthalmic trigeminal innervation when compared to other body regions. TSP magnitude was not different between the different body regions tested. Conclusions Our findings point to regional differences in endogenous pain inhibition and suggest that in otherwise healthy individuals, the trigeminal innervation is subjected to a weaker inhibitory pain control than other body regions. Such weaker endogenous pain control could play, at least in part, a role in mediating the high prevalence of trigeminal-related pain syndromes, including primary headaches and TMD pain.

CL-duo modules

In this paper, we introduce and study a new concept (up to our knowledge) named CL-duo modules, which is bigger than that of duo modules, and smaller than weak duo module which is given by Ozcan and Harmanci. Several properties are investigated. Also we consider some characterizations of CL-duo modules. Moreover, many relationships are given for this class of modules with other related classes of modules such as weak duo modules, P-duo modules.