Fluorescence Emission Wavelength Research Articles

An AIE-TICT fluorescence probe cascade responsive to H2S, polarity and viscosity to track microenvironment changes in cellular model of ischemia-reperfusion injury

BackgroundIschemia-reperfusion injury is a common cause of cardiovascular and cerebrovascular diseases. The reoxygenation during reperfusion leads to an overproduction of reactive oxygen species (ROS). As an antioxidant, H2S can scavenge ROS to inhibit oxidative stress and inflammatory reaction, thus attenuating ischemia-reperfusion injury. In this process, the changes of cellular microenvironment (polarity or viscosity) have not been fully discussed. In order to real-time track the changes of cellular microenvironment during the treatment of ischemia-reperfusion injury with H2S. It is necessary to develop highly selective and sensitive probes that can cascade response to hydrogen sulfide and cellular microenvironment. ResultsWe designed and synthesized a fluorescent probe TPEC-DNBS which can produce cascade response to H2S and microenvironment. An intermediate TPEC-OH is produced after highly selective and sensitive response to H2S, which can further respond to polarity and viscosity. In addition, due to the aggregation-induced emission (AIE) and twisted intramolecular charge transfer (TICT) effects, polarity can promote the fluorescence emission wavelength and intensity of TPEC-OH to produce double response characteristics, and its change trend (from weak green fluorescence at low polarity to strong red fluorescence at high polarity) is opposite to that of traditional polar probes (from strong green fluorescence at low polarity to weak red fluorescence at high polarity). Viscosity can only induce the change of fluorescence intensity. By constructing the cardiomyocyte model and hepatocyte model of ischemia-reperfusion, we further prove that after ischemia-reperfusion injury, the cells are in an environment of low polarity, and the microenvironment can be recovered after H2S treatment. SignificanceAn AIE-TICT fluorescence probe capable of cascading responses to H2S, polarity and viscosity was constructed by using tetraphenylethylene and coumarin moieties. This probe provides a more intuitive and convenient condition for real-time tracking the changes of cellular microenvironment (polarity or viscosity) before and after H2S treatment of ischemia-reperfusion injury.

Donor–Acceptor Type Solvatochromic Flavonoid Materials Fluorphores for Polarity Sensing and Real‐Time Temperature Monitoring

AbstractElectron donor–acceptor (D–A) molecules are emerging tools for designing sensitive luminogens, attracting significant research attention. However, due to poor quantum yields under certain conditions, current D–A fluorophores often suffer from fluorescence quenching in practical applications. Developing new D–A dyes and functional composites is crucial for expanding their applications. Here, a novel donor–acceptor type flavonoid dye molecule, namely “AFL”, exhibiting both polarity‐dependent fluorescence emission wavelength shift and viscosity‐dependent intensity change, for polarity measurement and temperature sensing is designed and synthesized. Because the intramolecular charge transfer (ICT) can be enhanced by a decrease of excited state energy caused in high‐polarity organic solvents, AFL undergoes fluorescence emission wavelength shift that shows fluorescence color response to solvents of different polarities and hence differentiates between them. Moreover, the actual temperature can be monitored by the as‐designed AFL@tetradecanoic acid (AFL@TA) sensor. By combining with TA, viscosity sensitively changes upon the temperature change of insoluble composites, thus realizing the fluorescence intensity response to temperature. Remarkable recyclable performance and satisfactory mechanical properties are integrated. This study provides a promising approach to developing a new D–A fluorescent probe and furnishes perspectives on the potential of such D–A type flavonoid material in stimuli‐responsive systems with a focus on visualization sensing technology.

Green hydrothermal approach for the synthesis of carbon quantum dots from waste tea bags for acrylamide detection in drinking water: A fluorescence assay validated by HPLC-PDA analysis

The study focused on converting tea bag waste into strong fluorescence carbon quantum dots (TBW-CQDs) for the detection of acrylamide in drinking water, antimicrobial activity, and photocatalytic degradation. The TBW-CQDs exhibited blue luminescence and maximum absorbance at 287 nm under UV light and distinctive fluorescence emission and excitation wavelengths at 425 nm and 287 nm, respectively. TBW-CQDs revealed a particle size of 8.12 ± 0.06 nm with a spherical morphology followed by an abundance of 59.29 % carbon and 39.82 % oxygen. For acrylamide extraction from water, the QuEChERS method was established, which exhibited a recovery rate of 97 to 99 %. The fluorescence-based sensor exhibited a low limit of detection of 0.35376 ppm, which was validated by HPLC-PDA (LOD 0.300688 ppm). TBW-CQDs degraded 90.62 % of indigo carmine and 93.19 % of methylene blue under bright sunlight. In conclusion, the fabricated TBW-CQDs provide a promising, cost-effective, and precise approach to acrylamide detection in drinking water.

A Novel Near-Infrared Tricyanofuran-Based Fluorophore Probe for Polarity Detection and LD Imaging.

In this paper, LD-TCF, a targeting probe for lipid droplets (LDs) with a near-infrared emission wavelength and large Stokes shift, was fabricated for polarity detection by assembling a donor-π-acceptor (D-π-A) molecule with typical twisted intramolecular charge transfer (TICT) characteristics. Surprisingly, the fluorescence emission wavelength of the newly constructed probe LD-TCF was stretched to 703 nm, and the Stokes shift was amplified to 126 nm. Furthermore, LD-TCF could specifically answer the change in polarity efficiently and did not experience interference from other biologically active materials. Importantly, LD-TCF exhibited the ability to target lipid droplets, providing valuable insights for the early diagnosis and tracking of pathophysiological processes underlying LD polarity.

Sensing the Small Change of Intermolecular Distance in Supramolecular Assembly by Using the Tunable Emission Wavelength of AIE-Active Luminogens.

The distinct molecular states - single molecule, assembly, and aggregate - of two ionic macromolecules, TPPE-APOSS and TPE-APOSS, are easily distinguishable through their tunable fluorescence emission wavelengths, which reflect variations in intermolecular distances. Both ionic macromolecules contain aggregation-induced emission (AIE) active moieties whose emission wavelengths are directly correlated to their mutual distances in solution: far away from each other as individual molecules, maintaining a tunable and relatively long distance in electrostatic interactions-controlled blackberry-type assemblies (microphase separation), or approaching van der Waals close distance in aggregates (macrophase separation). Furthermore, within the blackberry assemblies, the emission wavelength decreases monotonically with increasing assembly size, indicative of shorter intermolecular distances at nanoscale. The emission changes of TPPE-APOSS blackberry assemblies can even be visually distinguishable by eyes when their sizes and intermolecular distances are tuned. Molecular dynamics simulations further revealed that macromolecules are confined in various conformations by controllable intermolecular distances within the blackberry structure, thereby resulting in fluorescence emission with tunable wavelength.

Enhancing Two-Photon Excited Fluorescence of Metal-Organic Framework Single Crystals through Modulation of Inorganic Nodes.

Regulation of the two-photon excited fluorescence (TPEF) emission intensity and wavelength of metal-organic framework (MOF) crystals with similar constitutions presents a significant challenge. In this study, two MOFs, Zn-BTPPA and Cd3-BTPPA, were constructed using tetrakis(1,1'-biphenyl-4-carboxylic acid)-1,4-benzenediamine (H4BTPPA) as the organic ligand and mononuclear Zn and trinuclear Cd3 inorganic nodes, respectively. The incorporation of H4BTPPA within the MOF structures enables effective TPEF emission in both Zn-BTPPA and Cd3-BTPPA. The TPEF results show that Zn-BTPPA and Cd3-BTPPA exhibited strong emissions at 523 and 463 nm, respectively, when excited with a 780 nm laser. Moreover, Zn-BTPPA and Cd3-BTPPA exhibited much higher two-photon absorption cross sections, approximately 4.9 and 5.2 times higher than that of the reported dinuclear MOF, Cd2-BTPPA, with a similar composition, respectively. With different inorganic nodes, the stacking of chromophores, π···π interactions, and ligand geometry were found to correlate with the enhanced TPEF in Cd3-BTPPA and the blue-shifted TPEF in Zn-BTPPA. This work serves as an inspiration for designing efficient TPEF MOF materials based on the structure-property relationship.

Synthesis of Indole-Fused Pyrazino[1,2-a]quinazolinones by Copper(I)-Catalyzed Selective Hydroamination-Cyclization of Alkynyl-tethered Quinazolinones.

We described a copper(I)-catalyzed atom economic and selective hydroamination-cyclization of alkynyl-tethered quinazolinones to prepare a variety of indole-fused pyrazino[1,2-a]quinazolinones in good to excellent yields ranging from 39 %-99 % under mild reaction conditions. Control experiments revealed that coordination-directed method of quinazolinone moiety with copper(I) was important for the selective hydroamination-cyclization of alkynes at the N1-atom instead of N3-atom of quinazolinone. The reaction could be easily performed at gram scales and some prepared indole-fused pyrazino[1,2-a]quinazolinones with donating groups on the indole moiety showed a distinct fluorescence emission wavelength with blue shift under the acid conditions.

A long-wavelength fluorescent probe based on carbazole with AIE characteristics for the detection of ONOO− and its application

Peroxynitrite (ONOO−) is an important reactive oxygen species, and excess ONOO− in the body can lead to a range of diseases. Addressing the need for sensitive detection ONOO−, in this work, a highly sensitivity and selectivity long-wavelength fluorescent probe (named CIS) was designed for tracking ONOO− in biological cells. Probe CIS featured carbazole as the fluorophore and the CC double bond with two electron withdrawing –CN groups as the recognition site. The probe CIS exhibited the typical aggregation-induced emission (AIE) and intramolecular charge transfer (ICT) characteristics with the bright red fluorescent emission in high water fraction (fw ≥ 50 %) and negligible emission in low water fraction. The fluorescence emission wavelength of probe CIS is 631 nm, with a large Stokes shift (151 nm), which can sensitively detect ONOO− in DMSO: H2O (7:3, v/v) environment. Notably, the probe CIS exhibited a wide pH detection range (pH = 3–10), a low detection limit of 36.66 nM, high selectivity and minimal interference. Furthermore, probe CIS demonstrated excellent biocompatibility and low cytotoxicity, and can be used for the detection of exogenous ONOO− in 4T1 cells (Mouse breast cancer cells). These research results indicate that probe CIS has potential application prospects in the biological aspects.

“Silver effect” enhanced fluorescence for sensitive detection of crystal violet utilizing long wavelength emission bimetallic gold-silver nanoclusters

Crystal violet (CV), widely used as a fungicide in aquaculture, is susceptible to enrichment in fish and thus poses a threat to human health. Therefore, there is an urgent need for a simple, sensitive and cost-effective method to detect CV. To this need, duck egg white-stabilized gold-silver nanoclusters (DEW-AuAgNCs) with long wavelength fluorescence emission were synthesized via microwave method in 1 min. The raw materials used were easily available and environmentally friendly. And based on the Fluorescence resonance energy transfer (FRET) between DEW-AuAgNCs and CV, a fluorescence quenching method was established for CV detection. The detection limit of this method was 1.7 × 10−8 mol/L, which was lower than most of the reported methods. In addition, prominent performance of DEW-AuAgNCs with long wavelength emission can be applied for the detection of CVs in real organism.

Exploiting Mixed Valence Charge Transfer for Electrochromic and Electrofluorochromic Use.

An asymmetric mixed valence fluorophore with two different electron rich termini was investigated as a dual-role active material for electrochromism and electrofluorochromism. The fluorescence quantum yield (Φfl) and emission wavelength of the fluorophore were dependent on solvent polarity. The quantum yield of the material in an electrolyte gel, on a glass substrate and in a device was 40 %, 20 % and 13 % respectively. The fluorophore further underwent two near-simultaneous electrochemical oxidations. The first oxidation resulted in a 1000 nm red shift in the absorption to broadly absorb in the NIR, corresponding to the intervalence charge transfer (IVCT). Whereas the second oxidation led to a perceived green color at 715 nm with the extinction of the NIR absorbing IVCT. Owing to the dissymmetry of the fluorophore along with its two unique oxidation sites, the IVCT gives rise to a mixed valence transfer charge (MVCT). The coloration efficiency of the fluorophore in both solution and a device was 1433 and 200 cm2 C-1, respectively. The fluorescence intensity could be reversibly modulated electrochemically. The photoemission intensity of the fluorophore was modulated with applied potential in an operating electrochromic/electrofluorochromic device. Both the dual electrochromic and the electrofluorochromic behavior of the fluorophore were demonstrated.

Push-pull biphenyl and tolane derivatives as novel luminescent liquid crystals: synthesis and properties

ABSTRACT Addressing the current challenge of high melting points and long π-conjugated skeletons in luminescent nematic liquid crystals (LCs), this study explores the incorporation of push-pull dyes with biphenyl and tolane skeletons to extend fluorescence emission wavelengths into the visible light region. We synthesised push-pull-type biphenyl and tolane derivatives incorporating alkylamino groups as donors and cyano groups as acceptors. The thermal behaviour was investigated using optical polarisation microscopy, differential scanning calorimetry and X-ray diffraction. Notably, some molecules exhibited a nematic phase. The photophysical properties of the solution and solid states were also investigated. Furthermore, we formulated a room-temperature luminescent LC by blending several LCs. Importantly, the fluorescence in the nematic phase exhibited a red-shift compared to that observed in the solution and solid states. This study demonstrates the efficacy of the push-pull structure in the design of luminescent LCs. The findings of this study hold considerable implications for the development of advanced optoelectronic devices, particularly those requiring luminescent materials with tailored properties.

Development of a Pyrone-Fused Tricyclic Scaffold-based Ratiometric Fluorescent Probe for Al3+ Detection.

Aluminum (Al3+) is environmentally abundant and can harm living organisms in various ways, such as by inhibiting root growth, damaging faunal nervous systems, and promoting tumor cell proliferation. However, the dynamics of Al3+ in living organisms are largely unknown; thus, detecting Al3+ in the environment and organisms is crucial. Fluorescent probes are useful tools for the selective detection of metal ions. In particular, ratiometric fluorescent probes exhibit a detection response at two different maximum fluorescence emission wavelengths; which is advantageous for avoiding the influence of background fluorescence. A novel pyrone-fused tricyclic scaffold-based ratiometric fluorescent probe for detecting Al3+, ethyl 11-imino-1-oxo-3-phenyl-1H,11H-pyrano[4,3-b] quinolizine-5-carboxylate (PQ), was developed in this study. The PQ fluorescence blue shifted from 505 to 457nm upon the addition of Al3+. The blue shift was accompanied by a change in the fluorescence color of the PQ solution from green to blue. Fluorescence titration experiments demonstrated that the fluorescence intensity ratio at the two peaks of interest (457/505nm) increased in a concentration-dependent manner upon the addition of Al3+. Moreover, this study demonstrated that a PQ-soaked paper displays a visible color change under ultraviolet light upon exposure to Al3+. The above results suggest that PQ is an effective ratiometric probe for the detection of Al3+ in the environment. Future studies will be conducted to introduce various substituents and develop fluorescent probes by leveraging the fluorescence property of a pyrone-fused tricyclic scaffolds.

Cryo-Fluorescence Tomography as a Tool for Visualizing Whole-Body Inflammation Using Perfluorocarbon Nanoemulsion Tracers.

We explore the use of intravenously delivered fluorescent perfluorocarbon (PFC) nanoemulsion tracers and multi-spectral cryo-fluorescence tomography (CFT) for whole-body tracer imaging in murine inflammation models. CFT is an emerging technique that provides high-resolution, three-dimensional mapping of probe localization in intact animals and tissue samples, enabling unbiased validation of probe biodistribution and minimizes reliance on laborious histological methods employing discrete tissue panels, where disseminated populations of PFC-labeled cells may be overlooked. This methodology can be used to streamline the development of new generations of non-invasive, cellular-molecular imaging probes for in vivo imaging. Mixtures of nanoemulsions with different fluorescent emission wavelengths were administered intravenously to naïve mice and models of acute inflammation, colitis, and solid tumor. Mice were euthanized 24 h post-injection, frozen en bloc, and imaged at high resolution (~ 50 µm voxels) using CFT at multiple wavelengths. PFC nanoemulsions were visualized using CFT within tissues of the reticuloendothelial system and inflammatory lesions, consistent with immune cell (macrophage) labeling, as previously reported in in vivo magnetic resonance and nuclear imaging studies. The CFT signals show pronounced differences among fluorescence wavelengths and tissues, presumably due to autofluorescence, differential fluorescence quenching, and scattering of incident and emitted light. CFT is an effective and complementary methodology to in vivo imaging for validating PFC nanoemulsion biodistribution at high spatial localization, bridging the resolution gap between in vivo imaging and histology.

Enhancement of non-covalent interaction between soy protein isolate and quercetin by sodium alginate

Effects of sodium alginate (SA) on the non-covalent interaction between soybean protein isolate (SPI) and quercetin (Que) were investigated by multispectral technology, molecular docking and dynamics simulation technology. Structural alterations of the binary complexes were observed after SA addition, characterized by a red shift of maximum fluorescence emission wavelength. The introduction of 0.1% (w/v) SA led to a reduction of 12.3% in the α-helix and β-sheet structures, accompanied by 12.6% increase in the β-turn and random coil conformations. The binding of SA to SPI provided electrostatic interactions and facilitated the subsequent binding of SPI to Que. Molecular docking confirmed that hydrophobic interactions and electrostatic interactions were also the main driving force. Molecular dynamics simulation emphasized that the ternary complexes with SA exhibited greater stability compared to the binary ones. The foaming and emulsifying properties of SPI-Que complexes were enhanced by 33.76% and 68.28%, respectively, due to the addition of SA.

Optical characterization of black carbon-derived DOM: Implication for the fluorescence detection of fuel combustion products in marine waters

Among pollutants released from shipping, black carbon (BC), also known as soot carbon, is of great interest due to its impacts on climate, air quality, human health and ecosystems. BC emitted from ships may enter marine waters and partially transfer to the seawater dissolved phase. In this study, we investigated the optical properties (absorbance and fluorescence) of dissolved organic matter (DOM) derived from BC particles (DOMBC) emitted by ships, which were compared to those of DOMBC of other origins (diesel-powered industrial machine, biomass burning, urban dust), and to terrestrial and marine DOM (DOMTER, DOMMAR). Ship and diesel DOMBC displayed higher ratios of fluorescence maximum intensity to dissolved organic carbon concentration (Fmax/DOC), higher specific UV absorbance at 254 nm (SUVA254), and lower fluorescence emission wavelengths than the other tested materials. The parallel factor analysis (PARAFAC)-derived fluorophores of the ship and diesel DOMBC exhibited significant correlations with the concentration of dissolved black carbon (DBC), determined using the benzenepolycarboxylic acid (BPCA) method. Based on these results, we propose the Combustion indeX (COX), to help detecting and tracking ship/fuel combustion pollutions in marine waters.

Cys fluorescent probe precisely modified by fluorine revealing oxidative stress mechanism in Parkinson's disease

Oxidative stress is considered the fundamental pathophysiological mechanism of neurodegenerative diseases such as Parkinson's. Nuclear factor erythroid 2-related factor 2 (Nrf2), as a critical transcription factor for antioxidant response, activating its regulation of biothiol level is deemed an essential pathway for disease mitigation. Cysteine (Cys), an abundant and active biothiol, is a crucial molecule in this antioxidant pathway. To further elucidate these regulatory mechanisms, a specific, rapid, blood-brain barrier-crossing Cys fluorescent probe was demanded. Therefore, we selected isophorone as the parent compound, with bromoacetyl ester as the site responsive to Cys. By introducing fluorine only at the ortho position of bromoacetyl ester, utilizing fluorine's strong electron-withdrawing ability to activate the electrophilicity of methylene and carbonyl groups, probe WSX-F achieved specific and rapid response to Cys. Meanwhile, fluorine lengthened the probe's fluorescence emission wavelength and improved lipophilicity. In situ probe imaging revealed that the activated antioxidant mechanism for Nrf2 will up-regulate the level of Cys cells and the brain in Parkinson's disease, and the differential distribution of Cys in the Parkinson's brain region. The research provided theoretical guidance for preventing and treating Parkinson's disease.

Tunable quantum emitters on large-scale foundry silicon photonics

Controlling large-scale many-body quantum systems at the level of single photons and single atomic systems is a central goal in quantum information science and technology. Intensive research and development has propelled foundry-based silicon-on-insulator photonic integrated circuits to a leading platform for large-scale optical control with individual mode programmability. However, integrating atomic quantum systems with single-emitter tunability remains an open challenge. Here, we overcome this barrier through the hybrid integration of multiple InAs/InP microchiplets containing high-brightness infrared semiconductor quantum dot single photon emitters into advanced silicon-on-insulator photonic integrated circuits fabricated in a 300 mm foundry process. With this platform, we achieve single-photon emission via resonance fluorescence and scalable emission wavelength tunability. The combined control of photonic and quantum systems opens the door to programmable quantum information processors manufactured in leading semiconductor foundries.

A ratiometric fluorescence and colorimetry dual-signal sensing strategy based on o-phenylenediamine and AuNCs for determination of Cu2+ and glyphosate.

A ratiometric fluorescence sensing strategy has been developed for the determination of Cu2+ and glyphosate with high sensitivity and specificity based on OPD (o-phenylenediamine) and glutathione-stabilized gold nanoclusters (GSH-AuNCs). Water-soluble 1.75-nm size GSH-AuNCs with strong red fluorescence and maximum emission wavelength at 682nm were synthesized using GSH as the template. OPD was oxidized by Cu2+, which produced the bright yellow fluorescence oxidation product 2,3-diaminophenazine (DAP) with a maximum fluorescence emission peak at 570nm. When glyphosate existed in the system, the chelation between glyphosate and Cu2+ hindered the formation of DAP and reduced the fluorescence intensity of the system at the wavelength of 570nm. Meanwhile, the fluorescence intensity at the wavelength of 682nm remained basically stable. It exhibited a good linear relationship towards Cu2+ and glyphosate in water in the range 1.0-10 µM and 0.050-3.0µg/mL with a detection limit of 0.547 µM and 0.0028µg/mL, respectively. The method was also used for the semi-quantitative determination of Cu2+ and glyphosate in water by fluorescence color changes visually detected by the naked eyes in the range 1.0-10 µM and 0.30-3.0µg/mL, respectively. The sensing strategy showed higher sensitivity, more obvious color changes, and better disturbance performance, satisfying with the detection demands of Cu2+ and glyphosate in environmental water samples. The study provides a reliable detection strategy in the environment safety fields.

Brightness-constant solvatochromic dye for ratiometric fluorescent imaging of lipid dynamics in developing zebrafish

Lipids play a crucial role in the growth and development of organisms. However, the lack of effective chemical tools for lipid imaging has hindered a comprehensive understanding of the dynamic changes in lipid content and types in organisms. Here, we introduce a brightness-constant, lipid droplet-targeting ratiometric fluorescent probe called PAA for visualizing dynamic lipid changes during zebrafish growth and development. PAA was an ultra-stable dimer composed of two carbonylpyrenes. Despite variations in polarity, its absorption spectra, quantum yield and fluorescence brightness remained consistent. However, only fluorescence emission wavelength underwent redshift with increasing polarity. These properties enabled stable lipid imaging throughout the entire live zebrafish and allowed for the identification of lipid polarity using ratiometric fluorescence. Furthermore, PAA revealed changes of lipid composition in developing zebrafish. From 3–5 days post fertilization (dpf), zebrafish with undeveloped digestive organs exhibited a gradual decrease in overall lipid content and a reduction in lipid polarity. Subsequently, as the digestive organs fully developed (6–8 dpf), zebrafish were fed with paramecium, resulting in a gradual increase in overall lipid content and lipid polarity. Moreover, starvation experiments indicated that overall lipid content and polar lipids increased in zebrafish fed with paramecium. The successful application of PAA in visualizing lipid dynamics in zebrafish provides new avenues for studying lipid physiology and pathophysiology in living systems.

Blue fluorescent waterborne polyurethane‐based transition metal complexes with antibacterial activity

AbstractEfficient and stable blue fluorescent materials are the key components of light‐emitting devices and have received wide attention. Herein, transition metal complexes Zn(5‐AQ)2, Cu(5‐AQ)2, and Cd(5‐AQ)2 are prepared by using 5‐aminoquinoline (5‐AQ) as ligand and Zn2+, Cu2+, and Cd2+ as the central ions, respectively. Then, waterborne polyurethane (WPU)‐based transition metal complexes Zn(5‐AQ)2‐WPU, Cu(5‐AQ)2‐WPU, and Cd(5‐AQ)2‐WPU with different emission wavelengths of blue fluorescence are prepared by bonding Zn(5‐AQ)2, Cu(5‐AQ)2, and Cd(5‐AQ)2 to polyurethane chains as chain extenders, respectively. Noteworthily, with the increase of transition metal ion radius (Zn2+ < Cu2+ < Cd2+), the fluorescence emission wavelengths of the complexes showed different degrees of blue‐shift, and the blue‐shifted wavelengths showed a decreasing trend. Importantly, their fluorescence colors are blue‐shifted from blue‐green to blue with different emission wavelengths after accessing the polyurethane backbone, which has potential applications in the field of tuning white LED materials. The thermal decomposition temperatures of Zn(5‐AQ)2‐WPU, Cu(5‐AQ)2‐WPU, and Cd(5‐AQ)2‐WPU are above 220°C with good thermal stability, and their fluorescence lifetimes are prolonged by 8.817, 8.896, and 6.489 ns, respectively, compared with the complexes. Furthermore, WPU transition metal complexes with water as a dispersive medium have an antibacterial efficiency of up to 100% against Escherichia coli, which is an environmentally friendly antibacterial material with broad application prospects.