This paper presents a dual-band on-chip antenna-coupled high- Tc superconducting (HTS) Josephson-junction subterahertz (THz) fourth-harmonic mixer. The antenna utilizes a couple of different structured twin slots to enable the resonant radiations at two frequencies, and integrates a well-designed coplanar waveguide network for achieving good radiation coupling and signal isolation characteristics. The electromagnetic simulations show that coupling efficiencies as high as −4 and −3.5 dB are achieved for the 160- and 640-GHz operating frequency bands, respectively. Based on this dual-band antenna, a 640-GHz HTS fourth-harmonic mixer is developed and characterized in a range of operating temperatures. The mixer exhibits a measured conversion gain of around −18 dB at 20 K and −22 dB at 40 K, respectively. The achieved intermediate frequency bandwidth is larger than 23 GHz. These are the best results reported for HTS harmonic mixers at comparable sub-THz frequency bands to date.