AbstractRiverine water temperature (WT) is a crucial factor affecting habitat quality and ecological effect of aquatic ecosystems. To accurately quantify and classify WT variation features caused by climate change and reservoir construction and operation, a framework was developed that integrates multivariate vine copula model for accurately reconstructing the WT process and general evaluation indicators for comprehensively characterizing of WT variation. In this framework, month‐wise R‐vine copula models were employed to depict the multivariate dependence structure between WT and related hydrometeorological factors, and the change of WT process in the fluctuation range and thermal deviation was analogized as the change of simple harmonic wave in amplitude and phase. A testing‐oriented application of this framework in Yichang section of the Yangtze River highlighted that climate change and the Three Gorges Reservoir (TGR) dominated or participated in the fluctuation range changing and phase deviation of different monthly WT processes, as the ratios of affected months were 1.08:1 and 1.25:1 during the construction phase, and 1:2 and 1:1.28 during the operation phase. WT process also exhibited diverse monthly variation trends during construction and operation phases of the TGR. Therefore, it is inappropriate to neglect the impact of the TGR construction phase and climate change on WT variation. The proposed framework achieved systematic quantification and attribution analysis of WT variation, thereby providing an enhanced understanding of the variation characteristics of river thermal regimes under the individual and combined effects of climate change and artificial reservoir. Considering the significant influence of WT variation on aquatic organism reproduction, the identification of the sources and categories of monthly WT variation can also serve as a foundation for future targeted thermal and hydrological regime regulation, aiming to protecting aquatic species and preventing biodiversity loss.