Waterborne Concentrations Research Articles

Toxic effects of chromium chloride on hematology and histopathology of major carp (Labeo rohita)

Heavy metal contamination caused by human activities is a major threat to aquatic organisms. Therefore, a 28-day trial was designed to evaluate the hematological and histological changes in Labeo rohita exposed to chromium chloride. For this purpose, 240 fish (Labeo rohita) were randomly selected and divided into four groups. Three groups were treated with a sublethal waterborne concentration of chromium (37, 22, and 11 mgL−1); the fourth group was chromium-free control (0 mgL−1). Results demonstrated that noted values of hematological parameters, such as mean cell hemoglobin, red blood cells, hematocrit, platelets, and hemoglobin, were decreased significantly (P < 0.05) in experimental groups compared with control, while mean cell volume and white blood cells were increased. On the 28th day, a decline was observed in white blood cell values. The severity of histological lesions in the liver, kidney, and gills increased as the concentration and duration of chromium exposure increased. No histological change was observed in the control (T0), while T1 showed more severe changes than T2 and T3. Those results suggest that long-term exposure to chromium chloride causes adverse effects on the hematology and histology of major carp, Labeo rohita.

Novel DGT Configurations for the Assessment of Bioavailable Plutonium, Americium, and Uranium in Marine and Freshwater Environments.

Plutonium, americium, and uranium contribute to the radioactive contamination of the environment and are risk factors for elevated radiation exposure via ingestion through food or water. Due to the significant environmental inventory of these radioelements, a sampling method to accurately monitor their bioavailable concentrations in natural waters is necessary, especially since physicochemical factors can cause significant temporal fluctuations in their waterborne concentrations. To this end, we engineered novel diffusive gradients in thin-film (DGT) configurations using resin gels, which are selective for UO22+, Pu(IV + V), and Am(III) among an excess of extraneous cations. In this work, we also report an improved synthesis of our in-house ion-imprinted polymer resin, which we used to manufacture a resin gel to capture Am(III). The effective diffusion coefficients of Pu, Am, and U in agarose cross-linked polyacrylamide were determined in freshwater and seawater simulants and in natural seawater, to calibrate these configurations for environmental deployments.

Biological responses and toxicopathic effects elicited in Solea senegalensis juveniles by waterborne exposure to benzo[a]pyrene

Polycyclic aromatic hydrocarbons (PAHs) are priority contaminants in coastal and estuarine ecosystems under anthropogenic pressure. Although PAHs tend to accumulate in the sediment, toxicity for benthic flat fish such as soles may be caused by PAHs released from the sediment to the water column. Within this context, the present investigation aims at recognizing toxicopathic effects elicited after waterborne exposure to benzo[a]pyrene B[a]P, a model individual PAH compound, in juvenile Solea senegalensis. Sole juveniles were exposed to various concentrations of waterborne B[a]P for 3 and 7 days. Brain, liver, gills and gonad were the target tissues selected to determine biochemical and lysosomal biomarkers, and histopathology. Biological responses and toxicopathic effects were consistent with B[a]P concentration and exposure time. From day 3, hepatic catalase inhibition indicated potential oxidative effects of B[a]P. At day 7, contaminant exposure produced hepatic glutathione-S-transferase induction at low concentrations and inhibition at higher levels, evidencing a bell-shaped response. A clear gradient in lysosomal membrane destabilisation was observed in relation with B[a]P concentrations. Histopathological lesions were more frequent at day 7 and at higher contaminant levels. It seems that environmentally relevant waterborne concentrations of B[a]P (1000 ng/l) would suffice to cause toxicopathic effects on sole juveniles in relatively short exposure times. In agreement, the Integrative Biological Response index (IBR/n) indicated a dose-dependent decline in health condition upon exposure to B[a]P (IBR/nHighB[a]P > IBR/nMidB[a]P > IBR/nLowB[a]P > IBR/nDMSO > IBR/nControl). Overall, changes in antioxidant enzymes activity, lysosomal biomarkers and gill and liver histopathology are responsive early-warning signs of health disturbance in sole juveniles exposed to waterborne PAHs.

Chemically and Green Synthesized ZnO Nanoparticles Alter Key Immunological Molecules in Common Carp (Cyprinus carpio) Skin Mucus.

This study was conducted to compare the effects of commercially available (C) and green synthesized (GS) Zinc oxide nanoparticles (ZnO-NPs) on immunological responses of common carp (Cyprinus carpio) skin mucus. GS ZnO-NPs were generated using Thymus pubescent and characterized by UV–vis diffuse reflectance spectroscopy (DRS), Fourier-transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), scanning electron microscope (SEM), and energy-dispersive X-ray spectroscopy (EDX). Fish (n = 150) were randomly allocated into five groups in triplicate and received a waterborne concentration of 0% (control), 25%, and 50% of LC50 96 h of commercially available (C1 and C2) and green synthesized ZnO-NPs (GS1 and GS2) for 21 days. Results from XRD displayed ZnO-NPs with 58 nm in size and UV-vis DRS, EDX, and FT-IR analysis showed that some functional groups from plant extract bonded to the surface of NPs. The SEM images showed that ZnO-NPs have conical morphology. Acute toxicity study showed a higher dose of LC5096h for green synthesized ZnO-NPs (78.9 mg.L−1) compared to the commercial source (59.95 mg.L−1). The highest activity of lysozyme and alternative complement activity (ACH50) were found in control and GS1 groups. A significant decrease in alkaline phosphatase activity (ALP) was found in C1 and C2 groups compared to other treatments. Protease activity (P) was significantly decreased in the C2 group compared to the control and GS groups. Total immunoglobulin (total Ig) content was the highest in the control. In addition, total Ig in the GS1 group was higher than GS2. The exposure to ZnO-NPs lowered total protein content in all experimental groups when compared to control. Present findings revealed lower induced immunosuppressive effects by green synthesized ZnO-NPs on key parameters of fish skin mucus.

Acute mercury toxicity and bioconcentration in shrimp Litopenaeus vannamei juveniles: Effect of low salinity and chemical speciation

This study evaluated low salinity effect on acute Hg toxicity and bioconcentration capacity in L. vannamei juveniles (8.4 ± 0.7 g), because this species is frequently exposed to hypo-osmotic environments in its natural habitat, and in farming ponds. Hg LC50 values were 1723, 1272, 983 and 536 μg L−1 at salinity of 5 ppt (parts per thousand); 2203, 1740, 1340 and 873 μg L−1 at 10 ppt; and 7013, 5693, 1759 and 1534 μg L−1 at 25 ppt for 24, 48, 72 and 96 h, respectively. After 96 h, acute Hg toxicity in shrimp was significantly higher in salinity of 5 ppt than in 25 ppt; likewise, Hg bioconcentration in shrimp exposed to different Hg treatments was statistically greater in salinity of 5 ppt than in 25 ppt. The chemical speciation calculated in experimental waters suggested that neutral chemical Hg species (HgCl2 and HgClOH) were the most bioavailable because their fractions (51–62%) increased when salinity decreased. Therefore, the inverse relationship between Hg toxicity and salinity was due to osmotic stress and the neutral chemical Hg species fractions that increased at lower salinities. Hg bioconcentration factors indicated that the higher Hg waterborne concentrations were the most saturated uptake and storage mechanisms in shrimp. Thus, Hg concentrations in organisms did not increase in proportion to waterborne Hg concentrations in the three salinities. These results support the hypothesis of an effect of low salinity on Hg toxicity and bioconcentration capacity in L. vannamei. The safe Hg concentrations 5.4, 8.7 and 15.3 μg L−1 were proposed for shrimp exposed to salinity of 5, 10 and 25 ppt, respectively. This information allows recognizing risky environments for both wild and cultured healthy growth of these shrimp, which can help decision makers on coastal management and shrimp pond managers to have better water quality.

Organic contaminants sorbed to microplastics affect marine medaka fish early life stages development

The role of polyethylene microplastics 4–6 μm size (MPs) in the toxicity of environmental compounds to fish early life stages (ELS) was investigated. Marine medaka Oryzias melastigma embryos and larvae were exposed to suspended MPs spiked with three model contaminants: benzo(a)pyrene (MP-BaP), perfluorooctanesulfonic acid (MP-PFOS) and benzophenone-3 (MP-BP3) for 12 days. There was no evidence of MPs ingestion but MPs agglomerated on the surface of the chorion. Fish ELS exposed to virgin MPs did not show toxic effects. Exposure to MP-PFOS decreased embryonic survival and prevented hatching. Larvae exposed to MP-BaP or MP-BP3 exhibited reduced growth, increased developmental anomalies and abnormal behavior. Compared to equivalent waterborne concentrations, BaP and PFOS appeared to be more embryotoxic when spiked on MPs than when alone in seawater. These results suggest a relevant pollutant transfer by direct contact of MPs to fish ELS that should be included in the ecotoxicological risk assessment of MPs.

A common fungicide impairs stream ecosystem functioning through effects on aquatic hyphomycetes and detritivorous caddisflies

Fungicides can reach streams through runoff or adhered to leaf litter, and have the potential to adversely affect processes such as litter decomposition and associated communities. This study investigated the effects of chlorothalonil, a widely used fungicide, on litter decomposition, detritivorous invertebrates (larvae of the insect Sericostoma pyrenaicum) and aquatic hyphomycetes (AHs), using stream microcosms. We considered the single and combined effects of two exposure modes: waterborne fungicide (at two concentrations: 0.125 μg L−1 and 1.25 μg L−1) and litter previously sprayed with the fungicide (i.e., pre-treated litter, using the application dose concentration of 1250 μg L−1). We also assessed whether fungicide effects on invertebrates, AHs and decomposition varied among litter types (i.e., different plant species), and whether plant diversity mitigated any of those effects. Invertebrate survival and AH sporulation rate and taxon richness were strongly reduced by most combinations of fungicide exposure modes; however, invertebrates were not affected by the low waterborne concentration, whereas AHs suffered the highest reduction at this concentration. Total decomposition was slowed down by both exposure modes, and microbial decomposition was reduced by litter pre-treatment, while the waterborne fungicide had different effects depending on plant species. In general, with the exception of microbial decomposition, responses varied little among litter types. Moreover, and contrary to our expectation, plant diversity did not modulate the fungicide effects. Our results highlight the severity of fungicide inputs to streams through effects on invertebrate and microbial communities and ecosystem functioning, even in streams with well-preserved, diverse riparian vegetation.

Intra-Annual Changes in Waterborne Nanophyetus salmincola.

An analysis of daily water samples collected from an index site on Big Soos Creek, Washington indicated intra-annual differences in the concentrations of waterborne Nanophyetus salmincola. Waterborne concentrations, quantified as gene copies/L, peaked during the fall (October-November 2016), decreased to very low concentrations over the winter (January-March 2017), and then increased in the spring and throughout the summer. High waterborne concentrations of N.salmincola DNA (2 × 106 gene copies/L) corresponded with live N.salmincola cercariae (mean=3 cercariae/L) that were detected in companion water samples. Spikes in waterborne N.salmincola concentrations in October and November typically coincided with increases in streamflow; this combination resulted in elevated infection pressures during high water events in the fall. The peak in waterborne N.salmincola concentrations corresponded with an accompanying peak in tissue parasite density (metacercariae/posterior kidney) in Coho Salmon Oncorhynchus kisutch that were reared in the untreated water.

Mancozeb exposure results in manganese accumulation and Nrf2-related antioxidant responses in the brain of common carp Cyprinus carpio.

Manganese (Mn)-containing dithiocarbamates such as Mancozeb (MZ) have been shown to induce oxidative stress-related toxicity in rodents and humans. However, little is known about the neurotoxic effects induced by MZ in fish. In this study, carp (Cyprinus carpio) were exposed to non-lethal waterborne concentrations of MZ, and oxidative stress parameters as well as metal accumulation in fish brains were evaluated. The experimental groups were as follows: control, MZ 5mg/L, and MZ 10mg/L. Fish were exposed for 7days, and then brain was removed and prepared for subsequent analysis of antioxidant enzymes, reactive oxygen species (ROS), and expression of Nrf2 and phosphoNrf2. In parallel, manganese (Mn) levels were evaluated in blood and brain tissues. Mn levels were significantly increased in blood and brain of MZ-exposed carps. In addition, a concentration-dependent increase (p < 0.05) in ROS levels was observed in parallel to increments (p < 0.05) in the activity of major antioxidant enzymes, such as GPx, GR, and GST. On the other hand, significant decreases (p < 0.05) in CAT and SOD activities were observed. The expression of total and phosphorylated forms of Nrf2 was significantly (p < 0.05) upregulated in the brain of carps exposed to Mz when compared to the control, indicating an activation of the Nrf2 antioxidant pathway. Our study showed for the first time the activation of the Nrf2/ARE pathway and bioaccumulation of Mn induced by MZ exposure in fish species, highlighting important mechanisms of action and its toxicological impacts to aquatic organisms.

Toxicokinetic and toxicodynamic of depleted uranium in the zebrafish, Danio rerio

This study investigated the accumulation pattern and biological effects (genotoxicity and histopathology) to adult zebrafish (male and female) exposed to a nominal waterborne concentration of 20 μg L−1 of depleted uranium (DU) for 28 days followed by 27 days of depuration. Accumulation pattern showed that (i) DU accumulated in brain, (ii) levels in digestive tract were higher than those measured in gills and (iii) levels remained high in kidney, brain and ovary despite the 27 days of depuration period. Genotoxicity, assessed by comet assay, was significant not only during DU exposure, but also during depuration phase. Gonads, in particular the testes, were more sensitive than gills. The histology of gonads indicated severe biological damages in males. This study improved knowledge of ecotoxic profile of uranium, for which a large range of biological effects has already been demonstrated.

In vivo exposure of the marine sponge Chondrilla nucula Schmidt, 1862 to cadmium (Cd), copper (Cu) and lead (Pb) and its potential use for bioremediation purposes

The study aimed to test the cadmium (Cd), copper (Cu) and lead (Pb) bioaccumulation capacity of the marine sponge Chondrilla nucula and to measure the expression of metallothioneins (MTs) by a western blotting assay to explore metal tolerance and its potential use as a bioremediator in contaminated coastal areas. C. nucula was sampled in a protected marine area in order to perform experiments on organisms living in a healthy environment.Under laboratory conditions, the sponges were exposed to increasing concentrations of Cd, Cu and Pb in tanks filled with artificial seawater set up for the experiments. For each metal, three waterborne concentrations (mgL−1) of Cd (0.02–0.04-0.08), Cu (0.1–0.2-0.4) and Pb (0.2–0.4-0.8) were considered separately. SPSS 20.0 was used for statistical analysis. Significant differences in bioaccumulation across levels of each metal were highlighted. Moreover, the amount accumulated in sponges increased proportionately with increasing levels of exposure for each metal as well as MT expression after Cd and Pb exposure.The results demonstrate the efficiency of C. nucula in accumulating high levels of Cu, but a low tolerance to progressively higher concentrations for Cd and Pb. The study suggests the suitability of the use of this species in bioremediation plans for moderately contaminated environments. However, the biological response in the field should be verified by the use of transplanted organisms from a control site to a polluted site.

Endocrine disrupting effects of waterborne fluoxetine exposure on the reproductive axis of female goldfish, Carassius auratus

Evidence suggests that pharmaceuticals and personal care products reach urban watersheds, bioconcentrate in fish, and potentially disrupt physiological homeostasis. These impairments often affect hormone functions. Selective serotonin reuptake inhibitors (SRRIs) are increasingly studied with regards to their endocrine disrupting effects on teleost physiological processes, including reproduction. To examine whether FLX effects on the endocrine regulation of reproductive physiology in goldfish are sex-specific, we exposed sexually recrudescent female goldfish to two waterborne concentrations of FLX (0.54μg/L and 54μg/L) using an experimental design previously used for sexually mature male goldfish. To evaluate possible endocrine disrupting effects, we quantified the gonadosomatic index, circulating hormone concentrations (luteinizing hormone, LH; growth hormone, GH; 17-β estradiol, E2; and testosterone, T), and the expression of isotocin and vasotocin in the telencephalon, gonadotropin subunits and GH in the pituitary, and gonadotropin receptors, GH receptors, and aromatase in the ovary. Female goldfish exposed to 0.54μg/L FLX exhibited a significant decrease in circulating E2, and conversely, a significant increase in circulating LH and ovarian aromatase mRNA levels, suggesting disruption of E2-mediated feedback on LH release. These results, when compared with those previously observed in males, reveal that waterborne exposure to environmentally relevant levels of FLX sex-specifically disrupts the reproductive endocrine axis in goldfish, characterized by a decrease in E2 in females, and conversely, estrogen-like effects in males. These data emphasize the importance of studying the effect of endocrine disrupting chemicals on both sexes.

Ecotoxicity of water-soluble lectin from Moringa oleifera seeds to zebrafish (Danio rerio) embryos and larvae

The evaluation of ecotoxicity of mosquito larvicidal agents (such as the water-soluble lectin from Moringa oleifera seeds, WSMoL) is an essential step to establish the guidelines for their use. In this sense, this work evaluated the toxicity of WSMoL to Danio rerio embryos and larvae. Embryos were exposed to waterborne WSMoL (0.0125–0.2 mg mL−1) for 96 h and lethal and sub-lethal effects were observed every 24 h. In the bioassays with larvae, the individuals were exposed to the WSMoL (0.025–0.2 mg mL−1), mortality was recorded daily, and larval swimming velocities were analyzed after 72 h and 168 h of exposure. Additionally, acetylcholinesterase (AChE) activity of larvae was determined after 168 h of exposure. WSMoL LC50 values to embryos were 0.190, 0.133 and 0.049 mg mL−1 after 48, 72 and 96 h, respectively. No toxic endpoint was observed after exposure for 24 h. In addition, hatching was delayed and larval length at 96 h was reduced compared to the control. WSMoL LC50 to larvae were 0.21 and 0.135 mg mL−1, after 24 h and 96 h, respectively. Larvae exposed to 0.1 and 0.2 mg mL−1 showed a decrease in swimming speed and a significant reduction in AChE activity. In conclusion, WSMoL at waterborne concentrations needed for its use as a larvicide to A. aegypti causes lethal and sublethal effects to zebrafish embryos and larvae. Therefore, its use in waterbodies where there are non-target organisms is not recommended.

De novo assembly and analysis of changes in the protein-coding transcriptome of the freshwater shrimp Paratya australiensis (Decapoda: Atyidae) in response to acid sulfate drainage water.

BackgroundThe atyid shrimp Paratya australiensis occurs in surface freshwater habitats throughout eastern Australia and has been used to study the ecotoxicology of contaminants such as pesticides and metals. The acidification of surface water that can occur after acid sulfate material in soils and sediments is oxidised and subsequently re-wetted is a serious environmental issue in coastal regions and inland riverine floodplains worldwide. Solubilisation of soil-associated minerals can result in high waterborne concentrations of mineral salts and dissolved metals, which together with low pH represent a potential threat to aquatic ecosystems in affected regions. The aims of the present study were to gain insight into stress responses induced by exposure to acid drainage water (ADW) in P. australiensis by determining changes in the abundance of protein-coding transcripts and to generate a comprehensive transcriptomic resource to facilitate further research into gene regulation or protein structure and function in this species. Adult P. australiensis were exposed for 24 h to undiluted ADW, 50 % ADW diluted in river water, or to river water as control, and high-throughput mRNA sequencing (RNA-Seq) conducted on whole-body tissues. A reference transcriptome was generated using de novo assembly and putative protein-coding regions were identified and annotated. Changes in transcript abundance in response to ADW exposure were determined by aligning reads to the reference transcriptome and quantifying coverage.ResultsA high proportion of arthropod benchmarking universal single-copy orthologues were present in the reference transcriptome. Functions associated with cuticle biosynthesis and oxidative stress were significantly enriched in the lists of transcripts exhibiting differential abundance in either direction after exposure to 50 % or 100 % ADW. Transcripts involved in osmoregulation exhibited decreased abundance following exposure to ADW. The transcriptome contained full-length coding sequences for numerous proteins known to be involved in environmental response pathways, including two putative metallothioneins, four glutathione peroxidases and 19 nuclear receptors.ConclusionsThe results of the present study provide insight into stress response pathways induced in crustaceans by short-term exposure to multiple stressors present in ADW such as low pH, high salinity and dissolved metals, and represent a resource for future toxicogenomics and protein functional studies in P. australiensis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3208-y) contains supplementary material, which is available to authorized users.

Intersex and liver alterations induced by long-term sublethal exposure to 17α-ethinylestradiol in adult male Cnesterodon decemmaculatus (Pisces: Poeciliidae).

The aim of the present study was to assess the responses of the gonopodium morphology and the gonadal and liver histology of adult male Cnesterodon decemmaculatus to sublethal long-term exposure concentrations of 17α-ethinylestradiol (EE2). Two experiments were conducted exposing the fish to waterborne concentrations of EE2 ranging from 20 ng/L to 200 ng/L for 8 wk, 12 wk, and 16 wk. Intersex gonads were observed after 8 wk and 16 wk in fish exposed to 200 ng EE2/L and 100 ng EE2/L, respectively. Oocytes' development from testis germ cells and replacement of the efferent duct periodic acid-Schiff-positive secretion surrounding spermatozeugmata by parenchymal tissue and duct structure alterations were the major observed changes in the gonads. In contrast, no response was observed in the gonopodium morphology. Liver histology was also altered, showing increasing steatosis, single-cell necrosis to generalized necrosis, and disruption of acinar organization from 100 ng EE2/L to 200 ng EE2/L. In summary, the present results showed that although EE2 was not able to alter the morphology of a developed gonopodium, it was capable of inducing development of testicular oocytes in adult male C. decemmaculatus at environmentally relevant concentrations. Thus, externally normal but intersex C. decemmaculatus males would be expected in the wastewater-receiving streams that the species inhabits. According to the literature, the present study would be the first indicating estrogen-induced intersex in adult male poeciliid. Environ Toxicol Chem 2017;36:1738-1745. © 2016 SETAC.

Genotoxic Effects Induced by Cd(+2), Cr(+6), Cu(+2) in the Gill and Liver of Odontesthes bonariensis (Piscies, Atherinopsidae).

Genotoxic effects of Cd(+2), Cr(+6), and Cu(+2) on the gill and liver of the Argentinean Silverside (Odontesthes bonariensis) were studied using the comet assay and in relation with the metal tissue accumulation. Fish were exposed to three waterborne concentrations of each metal for 2 and 16 days. Genotoxicity was assessed by the single cell gel electrophoresis (comet assay). After 2 days, significant increase of the genetic damage index (GDI) was only observed in the gill of fish exposed to Cr(+6) and Cu(+2), and the LOECs were 2160 nM and 921.1 nM, respectively. The gill LOEC for Cd(+2) by 16 days was 9.4 nM. In the liver, LOECs were obtained only for Cd(+2) and Cr(+6) and were 9.4 and 2160 nM, respectively. The three metals were able to induce genotoxic effects at environmentally relevant concentrations and the gill was the most sensitive organ.

Critical Review: Toxicity of Dietborne Metals to Aquatic Organisms

This paper reviews dietborne toxicity of 13 metals (Ag, Al, As, B, Cd, Co, Cr, Cu, Mo, Ni, Pb, V, Zn) to aquatic biota. Of those, Ag, As, Cd, Cu, Ni, and Zn have caused dietborne toxicity in laboratory exposures when the dietborne concentrations resulted from exposure of the food to waterborne concentrations near toxicity thresholds. To facilitate merging this laboratory-based effects information with realistic exposure scenarios, concentrations of metals in water and food items should be surveyed in a variety of real-world freshwater and saltwater systems to determine dietborne:waterborne metal ratios and the chemical forms in which the metals occur.

Reach specificity in sediment E. coli population turnover and interaction with waterborne populations

Sediment-borne Escherichia coli can elevate waterborne concentrations through sediment resuspension or hyporheic exchange. This study sought to correlate hydrological, sediment transport, and water quality variables with: (i) the temporal stability of sediment E. coli populations [concentrations, strain richness and similarity (Raup–Crick index)]; and (ii) the contribution of sediment E. coli to the water column as defined through a library-dependent microbial source tracking approach that matched waterborne E. coli isolates to sediment E. coli populations. Three monitoring locations differing in their hydrological characteristics and adjacent upland fecal sources (dairy operation, low-density residential, and tile-drained cultivated field) were investigated. Sediment E. coli population turnover was influenced by sediment transport at upstream, high-energy reaches, but not at the downstream low-energy reach. Sediment contributions to the water column averaged 13% and 18%, and fecal sources averaged 17% and 21% at the upstream sites adjacent to dairy operations and low-density residential areas, respectively. Waterborne E. coli at the downstream site had low matches to E. coli from reach sediments (1%), higher matches to the upstream sediments (27% and 12%), and an average of 14% matches to the tile drained field. The percentage of waterborne E. coli matching sediment-borne E. coli at each stream reach varied in correlations to hydrological and sediment transport variables, suggesting reach-specific differences in the role of sediment resuspension and hyporheic exchange on E. coli transport.

Chronic nickel bioaccumulation and sub-cellular fractionation in two freshwater teleosts, the round goby and the rainbow trout, exposed simultaneously to waterborne and dietborne nickel

Rainbow trout and round goby were exposed for 30 days to waterborne and dietary Ni in combination at two waterborne concentration ranges (6.2–12μmol/L, 68–86μmol/L), the lower of which is typical of contaminated environments. The prey (black worms; Lumbriculus variegatus) were exposed for 48h in the effluent of the fish exposure tanks before being fed to the fish (ration=2% body weight/day). Ni in gills, gut, and prey was fractionated into biologically inactive metal [BIM=metal-rich granules (MRG) and metallothionein-like proteins (MT)] and biologically active metal [BAM=organelles (ORG) and heat-denaturable proteins (HDP)]. Gobies were more sensitive than trout to chronic Ni exposure. Possibly, this greater sensitivity may have been due to the goby's pre-exposure to pollutants at their collection site, as evidenced by ∼2-fold greater initial Ni concentrations in both gills and gut relative to trout. However, this was followed by ∼2–16× larger bioaccumulation in both the gills and the gut during the experimental exposure. On a subcellular level, ∼3–40× more Ni was associated with the BAM fraction of goby in comparison to trout. Comparison of the fractional distribution of Ni in the prey versus the gut tissue of the predators suggested that round goby were more efficient than rainbow trout in detoxifying Ni taken up from the diet. Assessing sub-cellular distribution of Ni in the gills and gut of two fish of different habitat and lifestyles revealed two different strategies of Ni bioaccumulation and sub-cellular distribution. On the one hand, trout exhibited an ability to regulate gill Ni bioaccumulation and maintain the majority of the Ni in the MT fraction of the BIM. In contrast goby exhibited large Ni spillovers to both the HDP and ORG fractions of the BAM in the gill. However, the same trend was not observed in the gut, where the potential acclimation of goby to pollutants from their collection site may have aided their ability to regulate Ni spillover to the BAM more so than in trout. Overall, chronic mortality observed in goby may be associated more with Ni bioaccumulation in gills than in gut; the former at either 4-d or 30-d was predictive of chronic Ni toxicity. BIM and BAM fractions of the goby gills were equally predictive of chronic (30-d) mortality. However, critical body residue (CBR50) values of the BIM fraction were ∼2–4× greater than CBR50 values of the BAM fraction, suggesting that goby are more sensitive to Ni bioaccumulation in the BAM fraction. There was insufficient mortality in trout to assess whether Ni bioaccumulation was predictive of chronic mortality.

Effects on Development, Growth Responses and Thyroid-Hormone Systems in Eyed-Eggs and Yolk-Sac Larvae of Atlantic Salmon (Salmo salar) Continuously Exposed to 3,3′,4,4′-Tetrachlorobiphenyl (PCB-77)

Thyroid hormones (triiodothyronine, T3; and thyroxine, T4) play significant roles in development, metamorphosis, metabolism, homeostasis, cellular proliferation, and differentiation, for which the effects are mediated through thyroid hormone receptors (TRα and TRβ). Similarly, the insulin-like growth factor (IGF) is involved in growth and development through regulation of somatic growth. This study was designed to examine the effects of the dioxin-like 3,3’,4,4’-tetrachlorobiphenyl (PCB-77) on responses related to growth and thyroid hormone system in eyed eggs and yolk-sac larvae of Atlantic salmon. Salmon eggs were continuously exposed to two waterborne concentrations of PCB-77 (1 or 10 ng/L) over a period of 50 d covering hatching and through yolk-sac absorption stages. Sampling was performed regularly throughout the exposure period and at different time intervals. Gene expression patterns were performed on whole-body homogenate at age 500, 548, 632, 674, and 716 dd (dd: day degrees) using quantitative polymerase chain reaction (PCR). Total T3 (TT3) and total T4 (TT4) were measured using radioimmunoassay (RIA). Data showed that 10 ng PCB-77 increased dioiodinase 2 (Dio2) at 500 dd and both PCB-77 concentrations decreased dio2 expression at 548 dd. PCB-77 elevated cellular TT3 at 500 dd and was lowered at 548 dd only at 10 ng. Otherwise, time-related reduction was not affected by PCB-77 exposure as observed for the rest of the exposure period. For TT4, 1 ng PCB-77 produced a rise at 500 dd, and an apparent concentration decrease at 548 dd, before a total inhibition at 632 dd. The IGF-1 and IGF-1R were variably affected by PCB-77. For IGF-2, PCB-77 produced a concentration-dependent increase at 548 dd, and thereafter an elevation (1 ng) and fall (10 ng) at 632 dd. TRβ mRNA demonstrated PCB-77 related increases during the exposure period, and this effect returned to control levels at 716 dd. For TRα, a rise was noted only after exposure to 10 ng PCB-77 at 500 dd. Overall, the present study demonstrates some possible growth and developmental consequences following exposure to PCB-77 during early life stages of Atlantic salmon.